全文获取类型
收费全文 | 132篇 |
免费 | 6篇 |
国内免费 | 6篇 |
出版年
2022年 | 1篇 |
2021年 | 4篇 |
2020年 | 3篇 |
2019年 | 3篇 |
2018年 | 7篇 |
2017年 | 4篇 |
2016年 | 1篇 |
2015年 | 3篇 |
2014年 | 14篇 |
2013年 | 16篇 |
2012年 | 9篇 |
2011年 | 9篇 |
2010年 | 2篇 |
2009年 | 4篇 |
2008年 | 3篇 |
2007年 | 8篇 |
2006年 | 10篇 |
2005年 | 7篇 |
2004年 | 3篇 |
2003年 | 5篇 |
2002年 | 4篇 |
2000年 | 1篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1988年 | 1篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 3篇 |
1979年 | 1篇 |
排序方式: 共有144条查询结果,搜索用时 218 毫秒
71.
INTRODUCTION: We studied the migration pattern, morphology and viability of cells suspended in five different fibrin glues. Besides this, the behaviour of chondrocytes seeded on porous matrices comprising different collagen types sealed with fibrin glue was investigated. MATERIAL AND METHODS: In an experiment A, cell suspension (0.5x10(6) cells) was incubated with different fibrin glues. Experiment B was set up to evaluate chondrocytes migration either through a collagen I/III (Chondro-Gide, Geistlich Biomaterials, Switzerland) or collagen II matrix sealed with different fibrin glues in a perfusion chamber system. Analysis were performed by lightmicroscopy (Mayer's hematoxylin-eosin; Masson-Goldner; TUNEL test) and by transmission and scanning electron microscopy. All fibrin glues were measured for TGF-beta 1 and 2 with a specific ELISA. RESULTS: After incubation of cell suspension in autologous fibrin glue, the morphology of cells is chondrocyte-like. Spindly, process-bearing cells were seen in commercial fibrin glue. Cells suspended in commercial fibrin glue revealed a significant higher percentage of TUNEL positive cells compared to fibrin tissue adhesives mixed with autologous serum (p=0.006). The TGF-beta 1 and 2 concentration was significantly higher in partial autologous fibrin sealant (PAF) compared to their commercial counterparts (p=0.001). Cells seeded on the collagen I/III matrix retained their chondrocytic morphology, while in the type II collagen matrix the chondrocytes displayed a fibroblastic phenotype. The ratio of TUNEL positive cells for the collagen I/III matrix was significantly surpassed by the values, when a collagen II matrix was used (p=0.008). No ingrowth of cells was seen in any of the experimental conditions. CONCLUSION: Partial autologous fibrin glue and collagen I/III matrices are favourable in respect to migration pattern, morphology and viability, but definitive conclusions can only be drawn after in vivo studies. This will be addressed in future animal studies. 相似文献
72.
For providing their offspring females of the digger wasp species Ampulex compressa hunt cockroaches, paralyze them and attach as a rule one egg to the coxa of one of the mid legs of their prey. We observed the egg-laying behavior and examined with light- and scanning microscopy (i) nearly mature eggs from ovaries of freshly dissected females and (ii) eggs immediately after their deposition on the coxae of their prey. The length of the white bean-shaped eggs varied between 2.2 and 3.0 mm, their diameter between 0.66 and 0.72 mm, and their weight between 345 and 832 μg. The surface of fresh, untreated eggs shows even at higher magnifications (>20.000×) a smooth appearance. However, after conventional fixation, dehydration with ethyl-alcohol and critical-point drying the egg-surface exhibited a little bit texture. The eggs are at two-third of their underside glued to the coxa of the prey. With the naked eye the glue appears as a compact mass. The eggs may be mechanically removed from the substrate (their attachment site); however, in doing so the viscous attachment glue appears in a more fibrous consistence. The polypeptide composition washed off the egg surface and the glue revealed no similarities, whereas the molecular mass of two polypeptides were similar between glue and the Dufour's gland contents. 相似文献
73.
Hiroto Fujita Yusuke Inoue Masayasu Kuwahara 《Bioorganic & medicinal chemistry letters》2018,28(1):35-39
We found for the first time that a thrombin-binding DNA aptamer (TBA) is selectively entrapped in fibrin gels during the gel growth reaction catalyzed by thrombin. Furthermore, using this phenomenon, we successfully demonstrated multiple incorporation of amphiphilic aliphatic groups into fibrin gels via chemically modified TBA. 相似文献
74.
纤维蛋白(原)在动脉粥样硬化发病中的作用及机制 总被引:28,自引:0,他引:28
在高胆固醇饮食诱发的家兔动脉粥样硬化模型上,发现AS早期即出现高纤维蛋白原血症,血浆Fg和胆固醇水平呈正相关。组织免疫化学检查发现,Fg密布于血管内皮下和平滑肌细胞间质。 相似文献
75.
V. Nehls Rita Herrmann Mirja Hühnken Alois Palmetshofer 《Cell and tissue research》1998,293(3):479-488
Angiogenesis and coronary artery collateral formation can improve blood flow and thereby prevent myocardial ischemia. The
role of perivascular fibroblasts in neovascularization remains incompletely understood. Here we investigated the effects of
epicardial and myocardial fibroblasts on angiogenesis in vitro by using a serum-free microcarrier-based fibrin gel angiogenesis
system. To clearly distinguish between different cell types, we either stained endothelial cells or fibroblasts in the living
with 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indocarbocyanine-perchlorate (DiI). In cocultures, low numbers of heart fibroblasts
stimulated endothelial sprouting, and capillary growth was also induced by fibroblast-conditioned media, indicating a paracrine
mechanism. Capillary formation was decreased by increasing the density of fibroblasts in the cocultures, indicating contact-dependent
inhibition. Using time-lapse studies, it turned out that close contacts between fibroblasts and endothelial cells resulted
in rapid retraction of endothelial cells or, rarely, in cell death. Depending on the local ratio of fibroblasts to endothelial
cell numbers, fibroblasts determined the location of capillary growth and the size of developing capillaries and thereby contributed
to capillary network remodeling. In contrast to primary heart fibroblasts, NIH 3T3 fibroblasts did not display contact-dependent
inhibition of endothelial sprouts. NIH fibroblasts were frequently seen in close association with endothelial capillaries,
resembling pericytes. Contact-dependent inhibition of angiogenesis by epicardial fibroblasts could not be reversed by addition
of neutralizing anti-TGF-β1 antibodies, by addition of serum, of medium conditioned by hypoxic tumor cells or myocardium,
by various cytokines or by growing cocultures under hypoxic conditions. Our results implicate a pivotal role of periendothelial
mesenchymal cells for the regulation of microvascular network remodeling and collateral formation.
Received: 15 September 1997 / Accepted: 6 April 1998 相似文献
76.
77.
为构建小鼠噬菌体抗体库 ,以获得对人血纤维蛋白特异的抗体 ,由小鼠脾脏提取 m RNA,经反转录 PCR扩增出抗体重链、轻链可变区基因片段 ,将二者和一段编码十五肽 (Gly4 Ser) 3的 DNA接头借助重组 PCR组装成为单链抗体 (single- chain antibody,Sc Ab)基因 .将单链抗体基因插入噬菌体展示载体 p CANTAB- 5E,通过电击法转化大肠杆菌 TG1细胞 ,用辅助噬菌体 M1 3K0 7超感染 ,构建了库容量在 1 0 8以上的噬菌体单链抗体库 .利用亲和选择方法 (淘选 ) ,从噬菌体抗体库中选得血纤维蛋白特异的单链抗体 .模拟抗体成熟过程 ,用 DNA改组 (DNA shuffling)技术使抗体基因重新组合 ,构建新的改组抗体库 ,并从中选择到提高了亲和力的噬菌体单链抗体 .抗体基因在大肠杆菌中表达 ,表达蛋白经 Sephadex G- 75柱层析分离 ,得到初步纯化的单链抗体蛋白 . 相似文献
78.
纳豆激酶溶解血栓机制 总被引:19,自引:0,他引:19
根据已有文献报道,综述了关于纳豆激酶溶栓机制的研究进展,将纳豆激酶的溶栓机制归纳为以下四点:直接溶栓作用;刺激血管内皮细胞产生内源tPA;激活体内尿激酶原转变为尿激酶;通过降解和失活纤溶酶原激活剂的抑制剂(PAI1)调控纤溶作用 。 相似文献
79.
目的:探讨医用生物蛋白胶在甲状腺次全切除手术治疗中的作用。方法:2003年10月至2005年12月甲状腺次全切手术治疗的186例患者分成两组,其中98例应用生物蛋白胶,不放引流物,另一组组88例放置胶管或胶片引流。两组进行引流量、拆线时间等多项指标对比。结果:第Ⅰ组多指标优于或等于第Ⅱ组。结论:在甲状腺次全切除术应用医用生物蛋白胶后渗血渗液明显减少,可不放引流物,引流物引起的并发症减少。 相似文献
80.
The evolution of stress in coagulating blood is described by aMaxwell-like constitutive model. The evolution is essentiallyaffected by conversion of fibrinogen to fibrin monomer, fibrinpolymerization and its crosslinking. The modifying effects of theprocess result from the active constituents of blood plasma,blood cell concentration and intensity of flow. Interrelationof stress evolution and the kinetics of polymerization species these effects in terms of the order of the kinetics ofpolymerization and degree of fibrin polymerization and values of the constitutive coefficients. The results are presented for a normal blood, averaging the effect of blood constituents and blood platelets on coagulation. 相似文献