全文获取类型
收费全文 | 29704篇 |
免费 | 2730篇 |
国内免费 | 2985篇 |
出版年
2024年 | 50篇 |
2023年 | 373篇 |
2022年 | 524篇 |
2021年 | 1590篇 |
2020年 | 1077篇 |
2019年 | 1349篇 |
2018年 | 1244篇 |
2017年 | 1030篇 |
2016年 | 1300篇 |
2015年 | 1896篇 |
2014年 | 2231篇 |
2013年 | 2406篇 |
2012年 | 2812篇 |
2011年 | 2573篇 |
2010年 | 1630篇 |
2009年 | 1455篇 |
2008年 | 1631篇 |
2007年 | 1409篇 |
2006年 | 1335篇 |
2005年 | 1120篇 |
2004年 | 1003篇 |
2003年 | 917篇 |
2002年 | 848篇 |
2001年 | 601篇 |
2000年 | 493篇 |
1999年 | 433篇 |
1998年 | 279篇 |
1997年 | 232篇 |
1996年 | 224篇 |
1995年 | 184篇 |
1994年 | 229篇 |
1993年 | 144篇 |
1992年 | 145篇 |
1991年 | 107篇 |
1990年 | 106篇 |
1989年 | 90篇 |
1988年 | 85篇 |
1987年 | 53篇 |
1986年 | 50篇 |
1985年 | 50篇 |
1984年 | 35篇 |
1983年 | 27篇 |
1982年 | 23篇 |
1981年 | 5篇 |
1980年 | 3篇 |
1979年 | 5篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1973年 | 2篇 |
1971年 | 2篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
151.
研究了大蒜(Allium sativum L.)中期染色体的超微结构和RNP物质。常规染色表明,大部分染色体内部有低电子密度区,有的染色体中低电子密度区域较大而似孔洞。银染结果也证明了有大小不等的孔洞存在。Bernhard 染色显示,在染色体周边和染色体内部都有RNP分布。用NaOH 处理证明了Bernhard 染色法所显示的深染区确实含有RNA。RNP量的多少与EDTA 的分化时间呈负相关 相似文献
152.
Shukuro Araki Shigehiro Yi Tatsufumi Murakami Susumu Watanabe Shinichi Ikegawa Kiyoshi Takahashi Ken-ichi Yamarnura 《Molecular neurobiology》1994,8(1):15-23
To analyze the pathologic processes of amyloid deposition in type I familial amyloidotic polyneuropathy (FAP), mice were made
transgenic by introducing the human mutant transthyretin (TTR) gene(MT-hMet 30). An inbred strain of mouse, C57 BL/6, was
chosen. Transgenic mice were killed using ether anesthesia at 3-mo intervals up to 24 mo after birth. In these transgenic
mice, amyloid deposition started in the gastrointestinal tract, cardiovascular system, and kidneys and extended to various
other organs and tissues with advancing age. The pattern of amyloid deposition was similar to that observed in human autopsy
cases of FAP, except for its absence in the choroid plexus and in the peripheral and autonomic nervous systems.
We extracted the amyloid fibrils from kidneys of these mice with a human mutant TTR gene and analyzed them immunochemically
and electronmicroscopically. Deposited amyloid was shown to be composed of human mutant TTR and mouse serum amyloid P component.
Amyloid fibril from transgenic mice was morphologically and immunohistochemically similar to that of human FAP.
The most striking pathologic feature of the transgenic mice was the absence of amyloid deposition in the peripheral and autonomic
nervous tissues. Thus, other intrinsic factors may be involved in amyloid deposition in the nervous tissues of human FAP. 相似文献
153.
分布于细胞内线粒体及细胞质中的天门冬氨酸氨基转移酶(AST·EC·2·6·1·1)是人血清中含有的两种同工酶,分别称为AST-m和AST-c同工酶.AST-c电泳迁移率介于血清α-球蛋白与β-球蛋白之间.AST-m电泳迁移率相似于γ-球蛋白,琼脂糖凝胶电泳固蓝B染色法对m-AST检出率较低.用NBT显色法则可得到较好效果. 相似文献
154.
大血藤科植物的分类学研究 总被引:4,自引:0,他引:4
作者对大血藤科植物的花性、叶片内部结构形态、花粉形态、染色体核型及过氧化物酶和脂酶同工酶进行了比较研究,结果表明:1)大血藤科植物外形上的两性花,其雄蕊的形态退化、花药始终不开裂,为功能上的雌花,因此其花为单性,同株,同序或异序;12)首次指出大血藤属植物的染色体数为2n=2x=22,属小型染色体,与木通科植物2n=2x=32、30、28的染色体数明显不同,支持Stapf(1926)将其从木通科分出另立为科的观点;3)单叶和复叶可出现于同一植株上,而且在叶片内部结构、花粉形态、染色体核型、过氧化物酶及脂酶同工酶等性状上,大血藤与单叶血滕间均无实质性的差异,因此将单叶血藤归并于大血藤中。 相似文献
155.
156.
Yong Soo Park Zhong Ping Shi Sumihisa Shiba Cayuela Chantal Shinji Iijima Takeshi Kobayashi 《Applied microbiology and biotechnology》1993,38(5):649-655
Fuzzy reasoning was applied to control both ethanol and glucose concentrations in fed-batch cultures of baker's yeast. This fuzzy controller consisted of three membership functions (concentrations of dissolved oxygen (DO), ethanol and glucose) and 18 production rules. Fuzzy inference was carried out by IF {A is a and B is b,...#x007D;, THEN {C is c} from the on-line measured concentrations of DO, ethanol and glucose. When medium concentrations of ethanol and glucose in fed-batch culture of baker's yeast were set at 2 g/l and 0.2 g/l, both ethanol and glucose concentrations were controlled at 2.67±0.35 g/l and 0.27±0.25 g/l, respectively, ethanol production was reduced from 26 g/l to 34 g/l, cell yield increased from 0.38 to 0.53 g dry cell/g consumed glucose and ethanol yield decreased from 0.50 to 0.14 g ethanol/g consumed glucose, respectively, as compared with those of the glucose only control at 0.2 g/l. 相似文献
157.
鸡马立克氏病病毒B抗原片段在大肠杆菌中表达 总被引:1,自引:0,他引:1
鸡马立克氏病病毒BamHI基因文库I3质粒中含有编码B抗原膜外Domain的DNA序列。经ScaI和SphI双酶酶解I3质粒,分离获得764bpDNA片段,并克隆进M13mp19中。DNA序列测定分析表明克隆片段为MDV-B抗原基因的494-1258bp部分序列。进一步分离NcoI-HindIII部分基因片(530bp),克隆于PLPromoter控制下的含有修饰型cIts857基因的表达载体中, 相似文献
158.
正常细胞转化成癌细胞后,其表型发生了一系列不同于正常细胞的变化,成为肿瘤细胞的标志。Gold和Freeman(1965)用人结肠癌组织的抽提物免疫兔,发现有些用人正常结肠组织吸收后的抗血清能够与肿瘤组织和胚胎肠道抽提物起反应,但不与正常组织抽提物起反应,由于这种抗原最初被发现在胚胎组织,故名为癌胚抗原(embryonic carcinoma antigen,简称CEA)。用敏感的放射免疫或免疫酶标方 相似文献
159.
Yi Sun Mahendra S. Rao Richard E. Zigmond Story C. Landis 《Developmental neurobiology》1994,25(4):415-430
Vasoactive intestinal peptide (VIP) expression increases in sympathetic neurons when they are grown in dissociated cell or explant cultures and when they are axotomized in vivo. In dissociated cell culture, the magnitude of the VIP increase was reduced when nonneuronal cells were removed and medium conditioned by ganglionic nonneuronal cells increased VIP in neuron-enriched cultures. Antiserum Against cholinergic differentiation factor (also leukemia inhibitory factor; CDF/LIF), but not against ciliary neurotrophic factor, immunoprecipitated this activity. Medium conditioned by sympathetic ganglion explants also contained a VIP-stimulatory molecule that was immunoprecipitated by CDF/LIF antiserum, and CDF/LIF antiserum partially blocked VIP induction in explants. CDF/LIF mRNA was increased in dissociated cell cultures, in ganglion explants and in vivo after axotomy. Our results suggest that CDF/LIF released from ganglionic nonneuronal cells plays an important role in regulating VIP after axotomy. 1994 John Wiley & Sons, Inc. 相似文献
160.