大蒜中期染色体内的核糖核蛋白物质 |
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引用本文: | 王学,郭世宜.大蒜中期染色体内的核糖核蛋白物质[J].Acta Botanica Sinica,1994,36(7):494-496. |
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作者姓名: | 王学 郭世宜 |
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作者单位: | 南开大学生物系!天津300071 |
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摘 要: | 研究了大蒜(Allium sativum L.)中期染色体的超微结构和RNP物质。常规染色表明,大部分染色体内部有低电子密度区,有的染色体中低电子密度区域较大而似孔洞。银染结果也证明了有大小不等的孔洞存在。Bernhard 染色显示,在染色体周边和染色体内部都有RNP分布。用NaOH 处理证明了Bernhard 染色法所显示的深染区确实含有RNA。RNP量的多少与EDTA 的分化时间呈负相关
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关 键 词: | 染色体 核糖核蛋白 大蒜 |
CHROMOSOMAL RIBONUCLEOPROTEINS AT MITOTIC METAPHASE IN ALLIUM SATIVUM |
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Authors: | Wang Xue Guo Shi yi Song Lin sheng Fan Ting yu Wang Xiu ling and Zhang Zi li |
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Abstract: | Chromosomal ultrastructure and ribonucleoproteins (RNP) at mitotic metaphase in garlic (Allium sativum L.) were studied. Areas of low electron is density were observed in the centre of some chromosomes with double staining with Ag staining technic.These areas of low electronic density were further observed as perferated holes. With Bernhard staining technique RNP could be detected in the holes but not evenly distributed. RNA identification was further ascertained by NaOH treatment. The amount of RNP visualized in Bernhard staining technique gradually faded as the duration of EDTA treatment was in creased. |
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Keywords: | Chromosome Ribonucleoprotein Allium sativum |
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