福建沿海地区的景观生态建设途径探讨

景观生态学研究景观和区域尺度的资源、环境经营与管理问题 ,具有综合整体性和宏观区域性特色 ,并以中尺度的景观结构与生态过程关系研究见长[8] 。自 2 0世纪 80年代后期 ,景观生态学逐渐成为资源、环境生态方面研究的一个热点。福建沿海地区是指北从福鼎县南至诏安县的广大沿海县、市 ,行政上包括 33个县市行政单位。该区域是福建省经济最为活跃 ,发展速度最快的地区 ,近 2 0年来经济年均增长率达 18% ,随着经济的发展 ,带来了一系列的环境问题 :城市生活污水、生活垃圾、工业废料和农业化肥等都成为福建省沿海地区严重的污染源。如何协调…     

卷带倍蚤一新亚种(蚤目:角叶蚤科)

记述倍蚤属Amphalius Jordan,1933-新亚种,卷带倍蚤孟达亚种Amphalius spirataenuis mengdaensis ssp.nov。采自青海省循化县孟达自然保护区的藏鼠兔Ochotona thibetana体上。模式标本存于青海省地方病防治研究所。     

蟹板茗荷在三疣梭子蟹体上的附着定向及种群变动

研究蟹板茗荷在三疣梭子蟹鳃及口肢上的定向、附着和种群特征 ,结果显示蟹板茗荷主要附着在三疣梭子蟹鳃的腹面 ,占 67.86% ,以在第五对鳃上的附着量最大。位于鳃腹面的蟹板茗荷主要以 0°方式定向 ,鳃背面的蟹板茗荷则主要以± 90°方式定向 ,鳃室壁上的蟹板茗荷主要以 0°方式定向。蟹板茗荷在不同的附着部位形态上有差异 ,口肢上的个体柄短、壳板厚而硬 ,鳃室里的柄长、壳板薄而软。附着于三疣梭子蟹鳃及口肢上的蟹板茗荷 ,其种群年龄结构和数量组成有明显的季节变化。     

山西历山自然保护区树鹨的繁殖习性

树鹨(Anthus hodgsoni)为中日两国政府协定保护鸟类。1999～2001年的4～10月,在历山保护区（东经111°51’～112°6’,北纬35°16’～35°27’）对该鸟的生态作了观察。选定富玉河8km的河谷阶地,每年4月上旬和10月下旬隔日观察树鹨最早迁来和最晚迁离的个体;每年5月和8月以富玉河（海拔1550～1750m）、后文堂（海拔1750～2050m）、舜王坪（海拔2050～2358m）3条路线统计其个体数量。     

逆转录-聚合酶链式反应检测果树RNA病毒

为调查主要果树携带苹果褪绿叶斑病毒(ACLSV)、苹果茎沟病毒(ASGV)、苹果茎痘病毒(ASPV)和李属坏死环斑病毒(PNRSV)的情况,以指示植物为试材,优化了四种病毒的逆转录-聚合酶链式反应(RT-PCR)检测体系.使用该优化体系检测了苹果树、樱桃树、桃树中携带上述四种病毒的情况.结果:建立了特异性强、灵敏度高、成本低的RT-PCR检测体系;同时又证明主要果树被这几种病毒单独感染和复合感染的程度均较高.     

麦白霉素发酵培养基的改进

研究了淀粉水解液全部替代葡萄糖作为碳源发酵麦白霉素的工艺条件。用正交实验得出摇瓶培养基消毒后的最佳基质浓度为总糖5.0g/100mL,还原糖3.8g/100mL,氨基氮60mg/mL,溶解磷300μg/mL。以50L发酵罐进行实验,得出了麦白霉素的发酵代谢曲线和淀粉的适宜水解条件。经30t发酵罐放大实验结果表明,与葡萄糖作碳源相比,用淀粉水解液作碳源发酵麦白霉素,发酵单位提高23.0％,生产成本降低38.0％。     

Inhibition of protein-tyrosine phosphatase stimulates the dynamin-dependent endocytosis of ROMK1.

We have previously shown that inhibiting protein-tyrosine kinase increased whereas inhibiting protein-tyrosine phosphatase (PTP) decreased renal outer medullary potassium channel 1 (ROMK1) channel activity (1). We have now used confocal microscopy, the patch clamp technique, and biotin labeling to further examine the role of tyrosine phosphorylation in regulating ROMK1 trafficking. Human embryonic kidney 293 cells were cotransfected with c-Src and green fluorescent protein-ROMK1, which has the same biophysical properties as those of ROMK1. Patch clamp studies have shown that phenylarsine oxide (PAO), an inhibitor of PTP, decreased the activity of ROMK1. Moreover, addition of PAO reduced the cell surface localization of green fluorescent protein-ROMK1 detected by confocal microscopy and diminished the surface ROMK1 density by 65% measured by biotin labeling. Also, PAO treatment significantly increased the phosphorylation of ROMK1. The notion that the effect of PAO is mediated by stimulating tyrosine phosphorylation-induced endocytosis of ROMK1 has also been supported by findings that mutating the tyrosine residue 337 of ROMK1 to alanine abolished the effect of PAO. Finally, the inhibitory effect of PAO on ROMK1 was completely blocked in the cells co-transfected with dominant negative dynamin (dynaminK44A). This indicates that the tyrosine phosphorylation-induced endocytosis of ROMK1 is dynamin-dependent. We conclude that inhibiting PTP increases ROMK1 phosphorylation and results in a dynamin-dependent internalization of the channel.     

Preheat treatment for Mycobacterium tuberculosis Hsp16.3: correlation between a structural phase change at 60 degrees C and a dramatic increase in chaperone-like activity.

The in vitro chaperone-like activity of Mycobacterium tuberculosis small heat shock protein Hsp16.3 was found to be dramatically enhanced to the same extent after preheat treatment at or over 60 degrees C. Structural analysis using gel filtration, native pore-gradient PAGE, nondenaturing PAGE, and far-UV CD spectroscopy consistently revealed no significant difference between the native and the preheated Hsp16.3 proteins. However, near-UV CD spectroscopy clearly demonstrated that the tertiary structure of preheated Hsp16.3 is quite similar to its native conformation, with a minor but significant difference. Further analysis using differential scanning calorimetry indicated that Hsp16.3 exhibited a structural transition near 60 degrees C. All these results together indicate that Hsp16.3 suffers a phase change at approximately 60 degrees C, which seem to remove a structural energy barrier for the protein to refold to a conformational status with increased chaperone-like activity.     

不同ES细胞系体定量神经分化的比较

利用视黄酸（RA）诱导小鼠胚胎干细胞通过悬浮类胚培养体外定量分化为神经样细胞,结果显示能够进行种系嵌合的MESPU22细胞系与不能进行种系统嵌合的MESPU13细胞系的体外神经分化比例在统计学上没有明显差异,此结果对于人的胚胎干细胞全能性的鉴定和检测有重要启示。     

三疣梭子蟹胚胎发育早期的组织学研究

对三疣梭子蟹（Portunus trituberculatus)胚胎发育早期（卵裂至原肠胚期）进行了组织学观察。结果发现：卵排至体外约５２h后开始卵裂,卵裂方式为表面卵裂,卵裂至２５６细胞时,胚胎发育进行了囊胚期。囊胚为实囊胚,囊胚后期,１６个排成例嗽叭形的预定内胚层细胞与聚在其附近的其他细胞一起内陷形成原肠。预定内胚层细胞脱离原肠后,进行１次切向分裂,形成卵黄细胞和内胚层细胞,与此同时,胚工细胞不断分裂,产生视叶原基和胸腹原基,不久,２个胞腹原基逐渐愈合形成胸腹突。随胚胎发育,在似桥细胞带上出现大颚原基、大触角原基,随后大大触角原基与视叶原基之间的腹中线上发生口凹,在小触角原基产生后,胚肥发育进入卵内无节幼体期。