Insect-resistant transgenic cabbage plants and their progenies

An insecticidal crystal protein gene of Bacillus thuringiensis was transferred into cabbage genome with the method of Agrobacterium infection. Cotyledons with petioles as explants were cocultivated with Agrobacterial suspension. Calli generated at the basis of petiole were subjected to selection on the MS medium containing 15-30 mg/L kanamycin (Km). About 5% explants produced calli growing continuously on the selective medium. Green shoots appeared on these calli when they were transplanted onto medium with Km and 6-BA for plant differentiation. The shoots were separated and cultivated on medium with kanamycin. About 80% shoots were rooted. Non-transformed control calli could not give normal shoots and roots and brownized and died gradually. Larvae of Pieris rapae showed poisonous symptoms: growth inhibition and mortality when fed with the leaf of the transgenic plants. About 80% of regenerated plants showed positive hybridization bands when their DNA were probed with crystal protein sequence of Bacillu     

单甲脒农药对模型池塘生态系统群落结构的影响

在３ｍ×１ｍ×１ｍ（Ｖ＝３ｍ３）含有底泥的模型池塘生态系统中研究单脒农药对水生生物群落结构的影晌。规定实验浓度力０、１．５、３．０、６．０和１２．０ｍｇ／Ｌ,每１５ｄ加入１次２５％单甲脒农药水剂,连续加入４次,实验进行２个多月。在实验浓度范围内,童甲脒农药对水生生物群落产生不同程度的影响。浮游生物比较敏感：加药后头几天内,种类、数量及多样性指数下降,浓度越大,影响越明显;大约１周以后,各处理组浮游生物群落逐步得到恢复,实验后期其数量甚至可超过对照水平,但群落结构发生改变,敏感种类少或消失,耐污种类增加,生物多样性降低。底栖生物比较耐污：处理槽大型水生植物的叶绿素含量有所减少,但其种类和生物量未见明显差异;底栖动物种类、数量也未见明显变化。微生物最耐污,在处理槽水层及沉积物中好氧异养菌数量有所增加,沉积物中厌气菌数量也有增加的趋势。青鱼对单甲脒农药较敏感,在１．５ｍｇ／Ｌ以下浓度尚能正常存活和繁殖。单甲脒农药水剂明显增加水体氮、磷含量,尤其磷酸盐含量高,使水体氮、磷比例失调,可能导致水体富营养化。根据综合指标分析,在规定单甲脒盆酸盐浓度＜１．５ｍｇ／Ｌ的实验条件下,水生生物群落结构未见明显改变．     

中国短痣蚜科新属新种记述（同翅目：蚜总科）

本文记述了短痣蚜科１新属——环短痣蚜属ＫｒｉｋｏａｎｏｅｃｉａＺｈａｎｇｅｔＱｉａｏ,ｇｅｎ．ｎ．及１新种——环短痣蚜ＫｒｉｋｏａｎｏｅｃｉａｃｉｒｃｕｌａＱｉａｏｅｔＺｈａｎｇ,ｓｐ．ｎ．,并探讨了该属的分类地位。模式标本存于中国科学院动物研究所昆虫标本馆内。环短痣蚜ＫｒｉｋｏａｎｏｅｃｉａＺｈａｎｇｅｔＱｉａｏ,新属模式种：环短痣蚜ＫｒｉｋｏａｎｏｅｃｉａｃｉｒｃｕｌａＱｉａｏｅｔＺｈａｎｇ,ｓｐ．ｎ．根据环短痣蚜新属腹部有缘瘤,有翅蚜前翅中脉分岔一次等特征,该新属应归于短痣蚜亚科内,与伪短痣蚜属ＡｉｃｅｏｎａＴａｋａｈａｓｈｉ,１９２１明显不同,而与短痣蚜属ＡｎｏｅｃｉａＫｏｃｈ,１７７５有较近的亲缘关系,不同则在于：新属次生感觉圈环形（后者卵圆形或椭圆形）;原生感觉圈有睫（无睫）;体背毛少（体背毛多）;体背无大型褐色斑纹（有）;前翅翅痣狭长,近长平行四边形（近三角形或椭圆形）。就次生感觉圈的形状,原生感觉圈有无睫,跗节Ｉ毛数的进化方向（Ｓｈａｐｏｓｈｎｉｋｏｖ,１９８１）,认为新属较短痣蚜属更为进化。分布：甘肃岷县（西寨２３００ｍ）。正模：有翅孤雌蚜Ｎｏ．８７３３－２－１－１,甘肃岷县（西寨２３００     

放射治疗对鼻咽癌患者细胞免疫功能的影响

应用抗人淋巴细胞单克隆抗体检测３２例鼻咽癌患者放疗前、后外周血Ｔ淋巴细胞亚群,并与１２例非癌人群（对照组）进行对比。结果：鼻咽癌患者ＯＫＴ３、ＯＫＴ４及ＯＫＴ４／ＯＫＴ８值与对照组相比明显降低,ＯＫＴ８显著上升（Ｐ＜０．０５）;放疗后ＯＫＴ３、ＯＫＴ４及ＯＫＴ４／ＯＫＴ８值进一步下降,ＯＫＴ８进一步升高（Ｐ＜０．０１）。提示鼻咽癌患者细胞免疫功能低下,放射治疗可进一步抑制鼻咽癌患者的细胞免疫功能。     

大水体池塘培育革胡子鲶稚鱼的研究

本文报道在云南南亚热燕山区大水体池塘中,用人工施肥培养浮游动物,作为革胡子鲶稚鱼的开口饵料及其形成夏花鱼苗的动物性饲料是可行的。对孵出四日龄的稚鲶进行培育,在水温２３．２－３０．３℃时,可使十七日龄稚鲶达到夏花水平,存活率达６０％以上,研究结果表明,四日龄稚鲶口宽为７６０－９００μｍ,开口期及生长前期主要摄取枝角类、桡足类和摇蚊幼虫。稚鲶生长后期,对轮虫、水生昆虫幼体及配合饲料的摄取量增加,而摄食     

小鼠着丝粒DNA探针对流式仪分选微核的染色体组成的初步研究

本文报道用作者建立的流式细胞仪红细胞微核自动检测技术,将染色体断裂剂丝裂霉素C(MMC)和非整倍体毒剂秋水仙碱(COM)诱导的大量微核分选在载玻片上,然后使用小鼠着丝粒γ-卫星DNA探针(约为234bp),对分选微核进行荧光原位杂交(FISH),以显示微核(MN)内着丝粒的情况,进而判定M N是由整条染色体还是由染色体断片组成。结果MN内着丝粒荧光阳性比例为COM50.1%,MMC 22.3%。两者相差显著,藉此方法可以准确有效地将两类毒剂区分开。 Abstract:Basis on auther’s new automatic flow cytometric technique for micronuclei,a lot micronuclei induced by clastogen Mitomycin C and aneugen colcemid were collected on slides using sorting function of flow cytometry,them the centromere Gamma satellite DNA probes of mouse (about 234bp) was used to do in situ hybridization for micronuclei,furthermore,the kinetochores of micronuclei can be showed,and the micronuclei which consist of the whole chromosomes or the chromosome fragments,can also be indicated.The results showed that 50.1% MN induced by COM and 22.3% MN induced by MMC had the positive fluorescent singles.There are significant difference between them,this means it is possible to distinglish clastogens and aneugens exactly and effectively with this method.     

光周期对粘虫生殖与飞翔影响的初步研究

在室内恒温条件下,测定了粘虫在短光照（ＬＤ８：１６）、中等光照（ＬＤ１２：１２）、长光照（ＬＤ１６：８）、光照逐日由长变短（ＬＤ１６：８－１２：１２）和逐日由为长（ＬＤ８：１６－１２：１２）等不同光周期条件下的发育进度,生殖力和飞翔能力。结果表明,光照由长变短使幼虫发育进度比其它处理平均缩短１ｄ以上,短光照、长变知道工换短、中等光照等条件下的成虫产卵前期均比长光照。短换长和短变和等处理蛾显著延长,     

EB病毒BNLF-1基因的分子生物学研究进展

位于EB病毒基因组U₅-TR区内的BNLF-1基因,其转译产物为潜伏膜蛋白(latent membrane protein1, LMP-1),由于LMP-1可以导致细胞转化并在EB病毒致癌过程中具有重要作用,因而成为近年来EB病毒分子生物学及相关肿瘤如人鼻咽癌、伯基特淋巴瘤、何杰金氏病等疾病病因发病学研究的热点,并取得了一批有重要意义的成果,文章从BNLF-1的基因结构及表达调控, LMP蛋白的结构及生化功能, LMP-1的生物学功能和LMP-1研究进行评述.     

Enhanced antitumor effects of tumor antigen-pulsed dendritic cells by their transfection with GM-CSF gene

To investigate the biological characterization and antitumor activitites of GM-CSF gene-transfected dendritic cells, the splenic dendritic cells were infected with GM-CSF recombinant replication-deficient adenoviruses in vitro . Their enhanced expression of B7 was demonstrated by FACS analysis, and more potent stimulatory activity was confirmed by allogeneic MLR. Immunization of dendritic cells pulsed with irradiated B16 melanoma cells induced sig-nificant CTL and enabled host to resist the challenge of wild-type B16 cells. When they were transfected with GM-CSF gene subsequently, the induced CTL activity was higher, and the produced protection against B16 cell challenge and therapeutic effect on the mice with preestablished pulmonary melastases more effective. These data suggest that the dendritic cells pulsed with tumor antigen then transfected with GM-CSF gene can be used as an effective vaccine in tumor immunotherapy.     

Expression of betaine aldehyde dehydrogenase gene and salinity tolerance in rice transgenic plants

Betaine as one of osmolytes plays an important role in osmoregulation of most high plants. Betaine aldehyde dehydrogenase C BADH) is the second enzyme involved in betaine biosynthesis. The BADH gene from a halophite, Atriplex hortensis, was transformed into rice cultivars by bombarment method. Totally 192 transgenic rice plants were obtained and most of them had higher salt tolerance than controls. Among transgenic plants transplanted in the saline pool containing 0.5% NaCl in a greenhouse, 22 survived, 13 of which set seeds, and the frequency of seed setting was very low, only 10% . But the controls could not grow under the same condition. The results of BADH ac-tivity assay and Northern blot showed that the BADH gene was integrated into chromosomes of transgenic plants and expressed.