Screening and identification of Shigella flexneri 2a virulence-related genes induced after invasion of epithelial cells

~~Screening and identification of Shigella flexneri 2a virulence-related genes induced after invasion of epithelial cells1. Jin, Q., Yuan, Z., Xu, J., Wang, Y., Shen, Y., Lu, W., Wang, J., Liu, H., Yang, J., Yang, P., Zhang, X., Zhang, J., Yang, G, Wu, H., Qu, D., Dong, J., Sun, L., Xue, Y, Zhao, A., Gao, Y., Zhu, J., Kan, B., Ding, K.. Chen, S., Cheng, H., Yao, Z., He, B., Chen, R., Ma, D., Qiang, B., Wen, Y, Hou, Y., Yu, J., Genome sequence of Shigella flexneri 2…     

Identification and expression profiling of putative odorant-binding proteins in the malaria mosquitoes, Anopheles gambiae and A.arabiensis

~~Identification and expression profiling of putative odorant-binding proteins in the malaria mosquitoes, Anopheles gambiae and A. arabiensis1. Curtis, C. F., Introduction 1: An overview of mosquito biology, behaviour and importance, in Olfaction in Mosquito-Host Interactions (eds. Bock, G. R.. Cardew, G.), New York: Wiley, 1996, 3-7. 2. Nighom, A., Hildebrand. J. G.. Dissecting the molecular mechanisms of olfaction in a malaria-vector mosquito, PNAS, 2002, 99(3): 1113-…     

双歧杆菌发酵对豆芽汁中大豆异黄酮含量及组分影响的研究

目的探讨双歧杆菌发酵对豆芽汁中大豆异黄酮含量及组分影响.方法采用HPLC法检测波长260 nm,测定豆芽汁中金省异黄素、大豆黄素的含量.结果表明通过发酵处理后,豆浆中总大豆异黄酮浓度没有明显的变化(P＞0.05);大豆异黄酮甙元的浓度较未经过发酵处理的豆浆组明显增加(P＜0.05);而大豆异黄酮糖甙的浓度却明显降低(P＜0.05).结论豆浆中的大豆异黄酮糖甙在双歧杆菌的β葡萄糖苷酶作用下水解为异黄酮甙元,异常酮甙元的浓度显著增加.     

The angiotensin Ⅱ type 1 receptor and receptor-associated proteins

INTRODUCTIONThe renin-angiotensin system (RAS) is consid-ered to be the major regulator of blood pressure)electrolyte balance and renal, neuronal as well as en-docrine functions related to cardiovascular control.The RAS is the key factor in most cases essential hy-pertension, as indicated by successes in treatment ofhypertensive patients with various angiotensin I con-verting enzyme (ACE) inhibitors and receptor block-ers. Renin was a central subject of intense investigation because of…     

《生物多样性》2000年度优秀论文奖

经我刊2/3以上编委投票,并经编委会讨论评选出2000年度优秀论文奖,公布如下： 　　黄宏文,龚俊杰,王圣梅,何子灿,张忠慧,李建强.猕猴桃属(Actinidia)植物的遗传多样性.2000,8(1)1～12 　　东秀珠,沈德龙,辛玉华.16S rDNA同源性所揭示的双歧杆菌与有关细菌的亲缘关系.2000,8（2）146～152 　　王剑伟,王伟,崔迎松.野生和近交稀有鮈鲫的遗传多样性.2000,8（3）241～247 　　本刊将对以上作者颁发荣誉证书、赠送2001年全年刊物并给予一定的物质奖励。 《生物多样性》编辑部     

The role of nitric oxide in cancer

Nitric oxide (NO) is a pleiotropic regulator, critical to numerous biological processes, including va-sodilatation, neurotransmission and macrophage-mediated immunity. The family of nitric oxide synthases (NOS) comprises inducible NOS (iNOS), endothelial NOS (eNOS), and neuronal NOS (nNOS). Interestingly, various studies have shown that all three isoforms can be involved in promoting or inhibiting the etiology of cancer. NOS activity has been detected in tumour cells of various histogenetic origins and has been associated with tumour grade, proliferation rate and expression of important signaling components associated with cancer development such as the oestrogen receptor. It appears that high levels of NOS expression (for example, generated by activated macrophages) may be cytostatic or cytotoxic for tumor cells, whereas low level activity can have the opposite effect and promote tumour growth. Paradoxically therefore, NO (and related reactive nitrogen species) may have both genotoxic and angiogenic pro     

红腹罗非鱼视网膜色素上皮及光感器的胚后分化

硬骨鱼胚后发育中,边缘生长区及胚胎裂缝代表神经形成的活跃位点,同时对新的神经胶质细胞和光感器(视锥和视杆细胞)的形成有积极作用。此项工作首次研究红腹罗非鱼(硬骨鱼类辐鳍亚纲)视网膜中视网膜色素上皮和光感器的胚后生长及分化。采用光学和透射电子显微镜术分析了四个大小不同的鱼群组。正如至目前为止,在已研究过的大多数其它硬骨鱼中所观察到的结果一样,我们研究的所有样本中,成年红腹罗非鱼的视网膜表现为气球样扩张。此外,尚具有一弯曲的、有开口的胚胎裂缝,表明眼的不对称性胚后生长。在胚后分化过程中,视网膜色素上皮表现为自外周向中央的梯度变化,即边缘生长区周围,该上皮仅含有球形的黑色素体,而在中央部位发育为长形的黑色素体及顶端突起。同样,发育中的光感受器亦表现为由侧面向中央逐步发展变化的程序。光感觉器先形成1内节及其附属的突起,随后内节分化为一椭圆体加一肌样结构,这两种成分与其它已研究过的硬骨鱼视网膜中观察到的胚胎发育程序大致相符。因此提供了另一线索：硬骨鱼胚后视网膜的生长极大地刺激(促进)了胚胎视网膜的发育。本研究表明：双体视锥细胞可能是发育中的单体视锥细胞裂开的结果[动物学报49(6)：813～818,2003]。     

大肠埃希菌性阴道感染小鼠模型的建立

目的 :建立大肠埃希菌定植的阴道感染小鼠模型。方法 :在阿莫西林溶液 (12 5mg/ml)冲洗小鼠阴道冲洗处理下 ,将致病性大肠埃希菌接种到小鼠阴道内。结果 :各组小鼠阴道冲洗液检出的平均对数值比较 ,经过阿莫西林溶液小鼠阴道冲洗处理后再接种大肠埃希菌的阿莫西林 +大肠埃希菌组 ,阴道内定植的大肠埃希菌数量 (8 18± 1 0 9)较经过生理盐水小鼠阴道冲洗处理后再接种大肠埃希菌组 (5 72± 0 6 8)明显增多 (P <0 0 5 ) ,而且小鼠阴道感染的症状明显 (P <0 0 5 )。结论 :通过抗生素处理后 ,再在小鼠阴道内接种大肠埃希菌 ,大肠埃希菌能够在小鼠阴道内得到定植 ,即建立起大肠埃希菌性阴道感染的小鼠模型。