超薄平板微型聚丙烯酰胺凝胶的等电聚焦电泳方法--介绍一种高效、快速的生物同工酶分析方法

介绍了一种简易、高效、快速的生物同工酶分析方法——超薄平板微型聚丙烯酰胺凝胶的等电聚焦电泳方法。该方法与目前国内外市场销售的同种用途等电聚焦的产品 (如BIO- RAD公司的 model1 1 1 Mini IEF Cell)相比 ,具有以下优点 :实验操作灵活 ,用途更广 ;谱带清晰 ;每次可实验的样本量大 ;电泳所需时间短 ;实验费用低 ;适用于多种酶系统的同工酶和等位酶分析 ;凝胶干燥不需任何设备且能长期保存 ;样本用量少。     

栗的 PGI遗传和多样性

采用超薄聚丙烯酰４胺平板凝胶等电聚焦电泳和单株后代法,分析了栗属种的ＰＧＩ同工酶的遗传,研究发现Ｐｇｉ位点（Ｐｇｉ－ｉ）主要有３个等位基因并呈共显性遗传。在栗属的自然居群中还检测了出现频率较少的另外２个等位基因。     

三种分析水稻F1代幼苗生长过程中酯酶同工酶变化的电泳方法比较

利用聚酯胶片作为凝胶支持物的超薄等电聚焦电泳(UTLIEF)研究水稻F1种子萌发过程中的酯酶同工酶多态性变化的结果表明,UTLIEF相比非变性不连续聚丙烯酰胺电泳(native-PAGE)和常规等电聚焦电泳(IEF)得到的酯酶同工酶图谱更清晰,酶带数目和强弱的多态性变化更高,用pH 2～9的两性电解质时等电聚焦的电泳效果较好.     

SDS-PAGE、同工酶等电聚焦和RAPD标记鉴定两系杂交水稻F1种子纯度对比研究

目的：探讨利用种子贮藏蛋白SDS—PAGE电泳、酯酶同工酶超薄等电聚焦电泳和RAPD分子标记鉴定五个两系杂交水稻组合F_1种子纯度的可行性。方法和结果：利用SDS—PAGE电泳技术未能找到所试五个组合各自的父本特征蛋白质带;利用酯酶同工酶超薄等电聚焦电泳技术和RAPD分子标记可找到所试五个组合的父母本特征酶带和RAPD标记,但酯酶同工酶的多态性同时也受种子萌发时间的影响。酯酶同工酶超薄等电聚焦电泳和RAPD分子标记可用于所试五个两系杂交水稻组合F_1种子纯度的鉴定。     

一种简单、快速、微量聚丙烯酰胺凝胶等电聚焦电泳方法

Bio-Rad公司的微量聚丙烯酰胺凝胶等电聚焦电泳(ModelⅢ Mini IEF Cell),不需要使用电极缓冲液,聚焦电泳时间仅用1.5小时,效果甚佳,但要使用该公司专有的凝胶支持薄膜,难于推广应用,我们经过试验,建立了一种简单、快速的微量凝胶等电聚焦电泳方法,40min完成等电聚焦过程,采用快速染色、脱色和干燥,将电泳图谱制成透明的薄膜,整个过程可以     

我国不同地区东亚钳蝎（Buthus martensii Karsch）毒在三种…

用ＳＤＳ－不连续聚丙烯酰胺凝胶板电泳、ｐＨ４．５－不连续聚丙烯酰胺凝胶板电泳和聚丙烯酰胺凝胶圆盘等电聚焦电泳,分离我国不同地区东亚钳蝎毒。其图谱表明：不同产地的东亚钳蝎毒蛋白的种类及含量存在一定的差异,地区相近,差异较小,反之亦然。     

一种改进的超薄聚丙烯酰胺等电聚焦电泳实验

目的:介绍一种自制、简易、高效的平板微型聚丙烯酰胺凝胶等电聚焦电泳方法。方法:将载玻片黏合作为制胶模具,剪滤纸制成上样芯子进行等电聚焦电泳。结果:电泳条带清楚,样品容量大,电泳时间短,操作性强。结论:此方法简单、有效、实用,所需凝胶量少,节约成本,值得推广。     

地衣芽孢杆菌β—甘露聚糖酶的纯化及其性质的研究

地衣芽孢杆菌１Ｂａｃｉｕｕｓ Ｌｉｃｈｅｎｉｆｏｒｍｉｓ）ＢＬ－３０６产生的胞外β－甘露聚糖酶经硫酸铵分级盐析,ＤＥＡＥ－纤维素柱层析。Ｓｅｐｈａｄｅｘ－Ｇ１００柱凝胶过滤和ＤＥＡＥ－纤维素柱再层析分离纯化,得到ＳＤＳ－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）均一样品。用ＳＤＳ－ＰＡＧＥ测得纯化后β－甘露聚糖酶分子量为２６０００道尔顿。用凝胶等电聚焦电泳（ＰＡＧＥＩＥＦ）测得等电点ＰＩ为５．０。该酶     

蜈蚣碱性蛋白SSmp—d的分离纯化及其部分理化性质的鉴定

少棘巨蜈蚣经９５％乙醇脱脂后,再经４℃水冷渗,水提液低温旋转浓缩,冻干,得到的冻干粉先后经过Ｓｅｐｈａｄｅｘ Ｇ－２５柱,等电聚焦制备电泳,再经Ｓｅｐｈａｄｅｘ Ｇ－１５０柱,Ｓｅｐｈａｄｅｘ Ｇ－１００柱,最后经ＨＰＬＣ制备得到一个纯的碱性蛋白,命名为ＳＳｍｐ－ｄ。该蛋白经ＨＰＬＣ、超薄等电聚焦电泳检验是均一的。采用ＨＰＬＣ和Ｐｒｏｔｅｉｎ－Ｐａｋ＾ＴＭ１２５柱测定其分子量为２４．６４ｋＤ。ＩＥ     

长沙地区两种常区蚯蚓及太平Ⅱ号蚓的EST,LDH同工酶研究

本文报道了长沙地区两种常见蚯蚓－日本杜拉蚓,舒脉环毛蚓以及人工养殖的太平Ⅱ号蚓为实验材料,应用聚丙烯酰胺凝胶圆盘电泳方法,分析ＥＳＴ,ＬＤＨ两种同工酶。实验结果表明ＥＳＴ同工酶酶谱复杂,ＬＤＨ同工酶具多态现象。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

Synthesis and antimicrobial studies of hydrazone derivatives of 4-[3-(2,4-difluorophenyl)-4-formyl-1H-pyrazol-1-yl]benzoic acid and 4-[3-(3,4-difluorophenyl)-4-formyl-1H-pyrazol-1-yl]benzoic acid

Microbial resistance to antibiotics is an unresolved global concern, which needs urgent and coordinated action. One of the guidelines of the Centers for Disease Control and Preventions (CDC) to combat antibiotic resistance is the development of new antibiotics to treat drug-resistant bacteria. In our effort to find new antibiotics, we report the synthesis and antimicrobial studies of 30 new pyrazole derivatives. These novel molecules have been synthesized by using readily available starting materials and benign reaction conditions. Some of these molecules have shown activity with MIC values as low as 0.78?µg/mL against four bacterial strains; Staphylococcus aureus, methicillin-resistant S. aureus, Bacillus subtilis, and Acinetobacter baumannii. Furthermore, active molecules are non-toxic to mammalian cell line.

     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Novel compounds with potent CDK9 inhibitory activity for the treatment of myeloma

Cyclin-dependent kinases (CDKs) and Polo-like kinases (PLKs) play key role in the regulation of the cell cycle. The aim of our study was originally the further development of our recently discovered polo-like kinase 1 (PLK1) inhibitors. A series of new 2,4-disubstituted pyrimidine derivatives were synthesized around the original hit, but their PLK1 inhibitory activity was very poor. However the novel compounds showed nanomolar CDK9 inhibitory activity and very good antiproliferative effect on multiple myeloma cell lines (RPMI-8226).