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1.
H. F. Ding F. Y. Yao G. X. Li M. S. Jiang R. F. Li X. D. Zhang W. Y. Wang F. Chen Y. Zhang 《Russian Journal of Plant Physiology》2009,56(4):532-539
The fragrance gene plays an important role in high-quality rice varieties and has been widely used in breeding programs. Using
a random sample of 370 individuals from an F2 segregating population developed from a cross between a japonica rice variety 9407 with fragrant flavor and an indica variety IRBB60, the fgr locus was mapped on chromosome 8 between SSR markers, PSM465 and RM1109, with genetic distances of 0.3 cM and 0.1 cM to respective
markers. These mapping efforts confirmed the previous mapping results. A large F3 mapping population with 7300 individuals was then developed from F2 plants, in which a small chromosomal region defined by the SSR markers, PSM465 and RM1109, was heterozygous. The analysis
of recombinants in the fgr region anchored the gene locus to an interval of 28 kb flanked by the left marker NS9 and the right marker L06. Sequence
analysis of this fragment predicted three open reading frames encoding putative 3-methylcrotonyl-CoA carboxylase, putative
isoleucyl-tRNA synthetase, and betaine aldehyde dehydrogenase (BADH2). The latter was presumed to be the candidate gene for
fragrance. This result will be very useful in molecular cloning of the fgr gene and marker-assisted transfer of the fgr gene in rice breeding programs.
Published in Russian in Fiziologiya Rastenii, 2009, vol. 56, No. 4, pp. 587–595.
This text was submitted by the authors in English. 相似文献
2.
Identification of microsatellite markers for fragrance in rice by analysis of the rice genome sequence 总被引:18,自引:0,他引:18
Cordeiro Giovanni M. Christopher Mandy J. Henry Robert J. Reinke Russell F. 《Molecular breeding : new strategies in plant improvement》2002,9(4):245-250
Several chemical constituents are important to the fragrance of cooked rice. However, the chemical compound 2-acetyl-1-pyrroline (AP) is regarded as the most important component of fragrance in the basmati- and jasmine-style fragrant rices. AP is found in all parts of the plant except the roots. It is believed that a single recessive gene is responsible for the production of fragrance in most rice plants. The detection of fragrance can be carried out via sensory or chemical methods, although each has their disadvantages. To overcome these difficulties, we have identified an (AT)40 repeat microsatellite or simple sequence repeat (SSR) marker for fragrant and non-fragrant alleles of the fgr gene. Identification of this marker was facilitated through use of both the publicly available and restricted access sequence information of the Monsanto rice sequence databases. Fifty F2 individuals from a mapping population were genotyped for the polymorphic marker. This marker has a high polymorphism information content (PIC = 0.9). Other SSR markers linked to fragrance could be identified in the same way of use in other populations. This study demonstrates that analysis of the rice genome sequence is an effective option for identification of markers for use in rice improvement. 相似文献
3.
Discovery of a new fragrance allele and the development of functional markers for the breeding of fragrant rice varieties 总被引:3,自引:0,他引:3
Weiwei Shi Yi Yang Saihua Chen Mingliang Xu 《Molecular breeding : new strategies in plant improvement》2008,22(2):185-192
The recessive fgr gene on chromosome 8 is associated with rice fragrance. It has been reported that this gene is a non-functional badh2 allele and that the functional Badh2 allele encoding putative betaine aldehyde dehydrogenase (BADH2) could render rice non-fragrant. Here we report the discovery
of a new badh2 allele and the development of functional markers for the badh2 locus. A total of 24 fragrant and ten non-fragrant rice varieties were studied and sequenced for their Badh2/badh2 loci. Of the 24 fragrant rice varieties, 12 were found to have the known badh2 allele (badh2-E7), which has an 8-bp deletion and three single nucleotide polymorphisms (SNPs) in exon 7; the others had a novel null badh2 allele (badh2-E2), which has a sequence identical to that of the Badh2 allele in exon 7, but with a 7-bp deletion in exon 2. Both null badh2 alleles are responsible for rice fragrance. Based on sequence divergence amongst the functional Badh2 and two null badh2 alleles, we developed functional markers which can be easily used to distinguish non-fragrant from fragrant rice and to differentiate
between two kinds of fragrant rice. These functional markers will find their usefulness in breeding for fragrant rice varieties
via marker-assisted selection.
Weiwei Shi and Yi Yang contributed equally to this work. 相似文献
4.
Molecular mapping of gene Gm-6(t) which confers resistance against four biotypes of Asian rice gall midge in China 总被引:2,自引:0,他引:2
S. K. Katiyar Y. Tan B. Huang G. Chandel Y. Xu Y. Zhang Z. Xie J. Bennett 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(6-7):953-961
The Chinese rice cultivar Duokang #1 carries a single dominant gene Gm-6(t) that confers resistance to the four biotypes of Asian rice gall midge (Orseolia oryzae Wood-Mason) known in China. Bulked segregant analysis was performed on progeny of a cross between Duokang #1 and the gall
midge-susceptible cultivar Feng Yin Zhan using the RAPD method. The RAPD marker OPM06(1400) amplified a locus linked to Gm-6(t). The locus was subsequently mapped to rice chromosome 4 in a region flanked by cloned RFLP markers RG214 and RG163. Fine
mapping of Gm-6(t) revealed that markers RG214 and RG476 flanked the gene at distances of 1.0 and 2.3 cM, respectively. Another gall midge
resistance gene, Gm-2, mapped previously to chromosome 4, is located about 16 cM from Gm-6(t), to judge by data from a segregating population derived from a cross between Duokang #1 and the Indian cultivar Phalguna
that carries Gm-2. We developed a PCR-based marker-assisted selection kit for transfer of the Gm-6(t) gene into Ming Hui 63 and IR50404, two parental lines commonly used in hybrid rice production in China. The kit contains
PCR primer pairs based on the terminal sequences of the RG214 and RG476 clones. Polymorphism between Duokang #1 and the hybrid
parental lines was found at these markers after digestion of the PCR products with specific restriction endonucleases. The
kit will accelerate introduction of gall midge resistance into hybrid rice in China.
Received: 18 May 2000 / Accepted: 9 March 2001 相似文献
5.
STS markers linked to Phoma resistance genes of the Brassica B-genome revealed sequence homology between Brassica nigra and Brassica napus 总被引:7,自引:0,他引:7
J. Plieske D. Struss 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(4):483-488
The RFLP and AFLP techniques are laborious and expensive and therefore of limited use for marker-assisted selection, demanding
a high throughput of samples in a short time. But marker-assisted selection is most useful for traits which are hard to score
on single plants and influenced by environmental factors. Four RFLP and three AFLP markers have been found to be linked to
genes of the B-genome of Brassica mediating resistance against Phoma lingam in oilseed rape. One RFLP and one AFLP marker were converted into three PCR-based STS markers: one of dominant, as well as
one of codominant inheritance separated in a standard agarose gel and a third one of codominant inheritance to be separated
in a polyacrylamide gel on an automated sequencer. As expected, the STS markers mapped at the same position as the original
RFLP and AFLP markers. The STS markers are efficient in marker-assisted backcross programs of the resistant B-genome/Brassica napus recombinant lines with most of the tested oilseed rape varieties and breeding lines. More than 90% of the tested oilseed
rape varieties and breeding lines exhibited no resistance marker alleles. The mapping results obtained with the markers, as
well as comparative sequencing of the marker alleles, indicate synteny and homology between the B-genome resistance gene donors
and B. napus in the region of the resistance genes. The location of the resistance genes in the B-genome/B. napus recombinant lines is most likely on the A genome. Thus the transfer of the B-genome resistance genes into Brassica campestris is also possible.
Received: 9 December 1999 / Accepted: 21 June 2000 相似文献
6.
S. Brunner B. Keller C. Feuillet 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(5-6):783-788
In many temperate areas of the world, leaf rust is becoming an important disease of barley. In the last decade, new races
of Puccinia hordei G. Otth have emerged which are virulent against the so-far most-effective race-specific resistance genes, such as Rph7. Marker-assisted selection greatly facilitates the pyramidization of two or more resistance genes in a single variety in
order to achieve a more comprehensive resistance. Such a strategy requires the development of efficient and reliable markers.
Here, we have developed a linkage map and found RFLP markers closely linked to the Rph7.g resistance gene on chromosome 3HS of barley. The receptor-like kinase gene Hv3Lrk that maps at 3.2 cM from Rph7.g was used to develop a PCR-based marker by exploiting a single nucleotide polymorphism. This marker was detected in 11 out
of 12 (92%) barley lines having Rph7 and represents a valuable tool for marker-assisted selection. In addition, the identification of markers flanking Rph7.g provides the basis for positional cloning of this gene.
Received: 1 December 1999 / Accepted: 28 February 2000 相似文献
7.
Drozd O. V. Strelnikov V.V. Babenko O. V. Zemlyakova V. V. Nemtsova M. V. Zaletaev D. V. 《Russian Journal of Genetics》2003,39(3):351-355
A PCR-based survey of allelic polymorphism of three microsatellite markers, DXS998, DXS548, and FRAXAC1, mapped to chromosome region Xq27.3, and two microsatellite markers, DXS8091and DXS1691 located on Xq28 was carried out using a series of DNA samples obtained from 98 unrelated individuals from Russia. The number of alleles detected on electrophregrams for each marker tested was 4, 6, 4, 5, and 3, respectively. The values of heterozygosity index for the markers examined were 0.65, 0.27, 0.38, 0.70, and 0.29, respectively. The observed distribution of the allelic frequencies for each microsatellite marker examined fitted Hardy–Weinberg expectations. The values of individualization potential determined for each marker were 0.24, 0.53, 0.43, 0.12, and 0.52, respectively. In the sample tested the genotype distribution with regard to above loci was determined. The perspectives of using the analyzed allelic polymorphisms for indirect DNA diagnostics of the monogenic diseases located in this chromosome region (X-linked mental retardations, FRAXA and FRAXE) as well as for human population genetics and personal identification is discussed. 相似文献
8.
Introgression of the Vf source of scab resistance and distribution of linked marker alleles within the Malus gene pool 总被引:1,自引:0,他引:1
G. J. King S. Tartarini L. Brown F. Gennari S. Sansavini 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(6):1039-1046
A chromosomal region originating from Malus floribunda 821 confers Vf scab resistance to many isolates of Venturia inaequalis. Twelve DNA markers located in this region were used to scan the equivalent of 31 cM in 98 Malus accessions. This allowed a molecular diagnosis of a source of resistance in apple germplasm with the aid of pedigree information,
and in the context of a limited marker survey representing other chromosomes. At least five marker alleles were present in
all scab-resistant breeding selections or varieties arising from M. floribunda. The validity of findings based on RAPD markers was confirmed with SCAR assays and Southern-hybridisation experiments. The
order of markers determined in previous mapping studies was confirmed and sets of recombinants identified that establish reliable
fine-mapping orders within 0.7 cM of the resistance locus. None of the marker alleles were present in the accessions that
are either susceptible or possess weak polygenic resistance to scab. The presence of some alleles corresponding to those present
at least 5.3 cM from Vf in M. floribunda was detected in some accessions. Other major sources of scab resistance do not appear to possess alleles in common with the
Vf region, which will simplify future allelism tests. The results are discussed in the context of the introgression of resistance
loci together with marker-assisted selection. The use of breeding pedigrees to assist in fine-scale mapping and map-based
cloning is discussed.
Received: 16 February 1999 / Accepted: 11 March 1999 相似文献
9.
Opportunities of marker‐assisted selection for rice fragrance through marker–trait association analysis of microsatellites and gene‐based markers 下载免费PDF全文
F. S. Golestan Hashemi M. Y. Rafii M. Razi Ismail M. T. M. Mohamed H. A. Rahim M. A. Latif F. Aslani 《Plant biology (Stuttgart, Germany)》2015,17(5):953-961
Developing fragrant rice through marker‐assisted/aided selection (MAS) is an economical and profitable approach worldwide for the enrichment of an elite genetic background with a pleasant aroma. The PCR‐based DNA markers that distinguish the alleles of major fragrance genes in rice have been synthesised to develop rice scent biofortification through MAS. Thus, the present study examined the aroma biofortification potential of these co‐dominant markers in a germplasm panel of 189 F2 progeny developed from crosses between a non‐aromatic variety (MR84) and a highly aromatic but low‐yielding variety (MRQ74) to determine the most influential diagnostic markers for fragrance biofortification. The SSRs and functional DNA markers RM5633 (on chromosome 4), RM515, RM223, L06, NKSbad2, FMbadh2‐E7, BADEX7‐5, Aro7 and SCU015RM (on chromosome 8) were highly associated with the 2AP (2‐acetyl‐1‐pyrroline) content across the population. The alleles traced via these markers were also in high linkage disequilibrium (R2 > 0.70) and explained approximately 12.1, 27.05, 27.05, 27.05, 25.42, 25.42, 20.53, 20.43 and 20.18% of the total phenotypic variation observed for these biomarkers, respectively. F2 plants harbouring the favourable alleles of these effective markers produced higher levels of fragrance. Hence, these rice plants can be used as donor parents to increase the development of fragrance‐biofortified tropical rice varieties adapted to growing conditions and consumer preferences, thus contributing to the global rice market. 相似文献
10.
Identification of AFLP fragments linked to seed coat colour in Brassica juncea and conversion to a SCAR marker for rapid selection 总被引:12,自引:0,他引:12
M. S. Negi M. Devic M. Delseny M. Lakshmikumaran 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(1-2):146-152
A Brassica juncea mapping population was generated and scored for seed coat colour. A combination of bulked segregant analysis and AFLP methodology
was employed to identify markers linked to seed coat colour in B. juncea. AFLP analysis using 16 primer combinations revealed seven AFLP markers polymorphic between the parents and the bulks. Individual
plants from the segregating population were analysed, and three AFLP markers were identified as being tightly linked to the
seed coat colour trait and specific for brown-seeded individuals. Since AFLP markers are not adapted for large-scale application
in plant breeding, our objective was to develop a fast, cheap and reliable PCR-based assay. Towards this goal, we employed
PCR-walking technology to isolate sequences adjacent to the linked AFLP marker. Based on the sequence information of the cloned
flanking sequence of marker AFLP8, primers were designed. Amplification using the locus-specific primers generated bands at
0.5 kb and 1.2 kb with the yellow-seeded parent and a 1.1-kb band with the brown-seeded parent. Thus, the dominant AFLP marker
(AFLP8) was converted into a simple codominant SCAR (Sequence Characterized Amplified Region) marker and designated as SCM08.
Scoring of this marker in a segregating population easily distinguished yellow- and brown-seeded B. juncea and also differentiated between homozygous (BB) and heterozygous (Bb) brown-seeded individuals. Thus, this marker will be
useful for the development of yellow seed B. juncea cultivars and facilitate the map-based cloning of genes responsible for seed coat colour trait.
Received: 2 October 1999 / Accepted: 11 November 1999 相似文献
11.
Shao Y Jin L Zhang G Lu Y Shen Y Bao J 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(5):1005-1016
Phytochemicals such as phenolics and flavonoids in rice grain are antioxidants that are associated with reduced risk of developing
chronic diseases including cardiovascular disease, type-2 diabetes and some cancers. Understanding the genetic basis of these
traits is necessary for the improvement of nutritional quality by breeding. Association mapping based on linkage disequilibrium
has emerged as a powerful strategy for identifying genes or quantitative trait loci (QTL) underlying complex traits in plants.
In this study, genome-wide association mapping using models controlling both population structure (Q) and relative kinship
(K) were performed to identify the marker loci/QTLs underlying the naturally occurring variations of grain color and nutritional
quality traits in 416 rice germplasm accessions including red and black rice. A total of 41 marker loci were identified for
all the traits, and it was confirmed that Ra (i.e., Prp-b for purple pericarp) and Rc (brown pericarp and seed coat) genes were main-effect loci for rice grain color and nutritional quality traits. RM228, RM339,
fgr (fragrance gene) and RM316 were important markers associated with most of the traits. Association mapping for the traits
of the 361 white or non-pigmented rice accessions (i.e., excluding the red and black rice) revealed a total of 11 markers
for four color parameters, and one marker (RM346) for phenolic content. Among them, Wx gene locus was identified for the color parameters of lightness (L*), redness (a*) and hue angle (H
o). Our study suggested that the markers identified in this study can feasibly be used to improve nutritional quality or health
benefit properties of rice by marker-assisted selection if the co-segregations of the marker–trait associations are validated
in segregating populations. 相似文献
12.
Markers for selection of the rice Xa21 disease resistance gene 总被引:8,自引:0,他引:8
C. E. Williams B. Wang T. E. Holsten J. Scambray F. de Assis Goes da Silva P. C. Ronald 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,93(7):1119-1122
Six molecular markers were mapped to a 7.4-cM region of rice chromosome 11 containing the Xa21 gene, which confers resistance to the pathogen Xanthomonas oryzae pv oryzae. Three markers, RG103, 248 and 818, co-segregated with Xa21 in a population of 1141 plants. Multiple copies of all marker loci were present within the region that was introgressed from Oryza longistaminata into O. sativa. The marker loci were cloned and primers were designed that defined sequence-tagged sites. Physical mapping of the three tightly linked central markers revealed that RG103, the marker that hybridizes to the Xa21 gene, resides on a separate DNA fragment from the other two markers.Disclaimer: Names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable. 相似文献
13.
Construction of a BAC contig containing the xa5 locus in rice 总被引:9,自引:0,他引:9
D. Yang A. Sanchez G. S. Khush Y. Zhu N. Huang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(7):1120-1124
The recessive gene xa5 confers resistance to bacterial blight in rice. To generate a physical map of the xa5 locus, three RFLP markers RG556, RG207 and RZ390, closely linked to xa5, were used to screen a rice bacterial artificial chromosome (BAC) library. The identified overlapping BAC clones formed two
small contigs which were extended to both sides by chromosome walking. The final physical map consisted of 14 BAC clones and
covered 550 kb. Genetic analysis with an F2 population showed that two RFLP markers 28N22R and 40F20R, derived from the BAC clones in the contig, flanked the xa5 locus. To further delimit the location of the xa5 locus, RFLP markers RG556 and RG207 were converted to sequence tagged sites and used to perform genetic analysis. The results
indicated that the xa5 locus was most likely located between RG207 and RG556. Among the BAC clones in the contig, one clone, 44B4, hybridized to
both RG207 and RG556. This suggests that BAC clone 44B4 carried the xa5 locus.
Received: 12 January 1998 / Accepted: 27 May 1998 相似文献
14.
K. Sakthivel N. Shobha Rani Manish K. Pandey A. K. P. Sivaranjani C. N. Neeraja S. M. Balachandran M. Sheshu Madhav B. C. Viraktamath G. S. V. Prasad R. M. Sundaram 《Molecular breeding : new strategies in plant improvement》2009,24(2):185-190
Fragrance development in rice has been reported due to a 8-bp deletion in the exon 7 of badh2 gene located on Chromosome 8S. Multiplex markers targeting the functional InDel polymorphism was earlier reported for genotyping
fragrance trait, but the marker was observed to be inconsistent and difficult to use. We have developed a simple, co-dominant,
functional marker for fragrance trait, which can be resolved in an agarose gel and validated in Basmati and non-Basmati aromatic
rice varieties and in a mapping population segregated for fragrance trait. The marker targets the InDel polymorphism in badh2 gene and amplifies 95 and 103 bp fragments in fragrant and non-fragrant genotypes, respectively. The newly developed marker
was highly efficient in discriminating all fragrant and non-fragrant genotypes and showed perfect co-segregation with the
trait of fragrance in the mapping population. We recommend the use of this simple, low-cost marker in routine genotyping for
fragrance trait in large scale breeding materials and germplasm. 相似文献
15.
Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice 总被引:35,自引:0,他引:35
S. Hittalmani A. Parco T. V. Mew R. S. Zeigler N. Huang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(7):1121-1128
Three major genes (Pi1, Piz-5 and Pita) for blast resistance on chromosomes 11, 6 and 12, respectively, were fine-mapped and closely linked RFLP markers identified.
New markers for Pi1 and Pita were found that were flanking the genes. The three genes were pyramided using RFLP markers. A PCR-based SAP (sequence amplified
polymorphism) marker was used to identify Piz-5 in the segregating population. The plants carrying the two- and three-gene combinations that were tested for resistance
to leaf blast in the Philippines and India indicated that combinations including Piz-5 have enhanced resistance than when it is present alone. The genes from the pyramided lines are at present being deployed
into agronomically superior ricevarieties by marker-aided selection (MAS).
Received: 20 June 1997 / Accepted: 14 September 1999 相似文献
16.
Ruangchai Juwattanasomran Prakit Somta Akito Kaga Sompong Chankaew Takehiko Shimizu Worawit Sorajjapinun Peerasak Srinives 《Molecular breeding : new strategies in plant improvement》2012,29(1):13-21
We have previously reported an association between a single nucleotide polymorphism (SNP) in exon 10 of GmBADH2 gene and fragrance in vegetable soybean [Glycine max (L.) Merr.] cultivar Kaori. The SNP causes amino acid substitution in a highly conserved motif of GmBADH2 protein, which
is necessary for functional activity of the protein. In this study, we sequenced GmBADH2 in another fragrant soybean cultivar Chamame and discovered a new fragrance allele, which has a 2-bp (TT) deletion in exon
10. The deletion causes a reading frame shift and introduces a premature stop codon, which could abolish protein function
and result in fragrance. The old and new fragrance-promoting alleles were designated Gmbadh2-1 and Gmbadh2-2, respectively. A simple and co-dominant functional marker was developed for genotyping Gmbadh2-2. The marker can discriminate between fragrant and non-fragrant soybeans and distinguish the two different fragrant soybeans,
and thus is useful for routine genotyping for the fragrance trait in breeding programs. Quantitative trait locus (QTL) mapping
in an F2 population using Chamame as the fragrance donor revealed that the location of the fragrance QTL nearly coincided with that
of the functional marker, confirming the association between GmBADH2 and fragrance in Chamame. 相似文献
17.
Microsatellite and sequence-tagged site markers diagnostic for the rice bacterial leaf blight resistance gene xa-5 总被引:14,自引:0,他引:14
M. W. Blair S. R. McCouch 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(1-2):174-184
Microsatellite and sequence-tagged site (STS) markers tightly linked to the bacterial leaf blight (BLB) resistance gene xa-5 were identified in this study. A survey was conducted to find molecular markers that detected polymorphisms between the resistant
(IRBB5) and susceptible (‘IR24’) nearly isogenic lines for xa-5, and between Chinsurah Boro II (CBII), an alternative source of xa-5, and a widely planted variety (‘IR64’) that lacks xa-5. Two F2 populations, from the crosses ‘IR24’×IRBB5 and CBIIבIR64’, were used to estimate linkage based on marker genotype and reaction
to disease inoculation with Xanthomonas oryzae pv. oryzae. Two RFLP clones, RZ390 and RG556, were found to co-segregate with xa-5 and were converted into STS markers. A microsatellite marker, RM390, was developed based on a simple sequence repeat in the
5′ untranslated region of the cDNA probe, RZ390, and found to co-segregate with resistance. Two other microsatellites, RM122
and RM13, were located 0.4 cM and 14.1 cM away from xa-5. A germplasm survey of diverse lines containing BLB resistance genes using automated fluorescent detection indicated the
range of allelic diversity for each of the microsatellite loci linked to xa-5 and confirmed their usefulness in following genes through the narrow crosses typical of a breeding program. The limited number
of alleles observed at the microsatellite loci linked to the resistance gene in 35 xa-5-containing accessions suggested either a single ancestral origin or a few independent origins of the xa-5 gene. PCR-based markers, like the ones developed in this study, are economical and easy to use, and have applicability in
efforts to pyramid the recessive xa-5 gene with other BLB resistance genes.
Received: 27 September 1996/Accepted: 7 February 1997 相似文献
18.
A PCR-based marker technique was developed to discriminate between morphologically similar split seed of vetch (Vicia sativa) and lentil (Lens culinaris subsp.culinaris). Sequence tagged microsatellite site (STMS) markers were more discriminatory than markers produced from the nontranscribed
spacer (NTS) region of the 5S ribosomal RNA gene. A sequence characterized amplified region (SCAR) marker, developed from
the 5S rRNA NTS region, was sensitive when resolved on agarose. However, the fluorescent-labeled 5S rRNA SCAR marker was unable
to discriminate between vetch and lentil, probably because of the low copy number of the marker, and was not visualized on
agarose. An STMS primer-pair (PSMPSAD123), developed from field pea, was able to discriminate split red cotyledon vetch from
split red cotyledon lentil because it produced specific markers at 563 bp for lentil and 353 and 474 bp for vetch. The vetch-specific
STMS marker was conserved among all species of theVicia genus used in this study and was sensitive enough to discriminate both on agarose gels and on polyacrylamide gel-based fluorescent
systems. The fluorescent-tagged STMS analysis revealed peaks for vetch and lentil at the expected sizes in admixtures of milled
vetch and lentil seeds, and it was sensitive enough to detect one vetch seed in 1999 lentil seeds. The development of PCR-based
tests for detecting the level of vetch seed contamination in lentil export seed may provide a method for quality assurance
of export lentil seed. 相似文献
19.
Mapping of the Rf-3 nuclear fertility-restoring gene for WA cytoplasmic male sterility in rice using RAPD and RFLP markers 总被引:24,自引:0,他引:24
G. Zhang Y. Lu T. S. Bharaj S. S. Virmani N. Huang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(1):27-33
The cytoplasmic male sterility (CMS) of wild-abortive (WA) cytoplasm has been widely used for breeding hybrid rice. Two restorer
genes for the CMS have been found by traditional genetic analysis. To tag the restorer genes we used a set of near-isogenic
lines (NILs) of Zhenshan 97 carrying different genotypes for fertility restoration from IR24, to perform RAPD analysis. From
the survey of 720 random primers, six RAPD markers were identified to be associated with Rf-3. Three of these OPK05-800, OPU10-1100 and OPW01-350, were mapped on chromosome 1. Two populations from the crosses between Zhenshan 97 A and a near-isogenic restorer line ZSR21 and between Zhenshan 97 A and
IR24 were used for mapping Rf-3. The three RAPD markers and three RFLP markers, RG532, RG140 and RG458, were found to be closely linked to Rf-3 in the two populations. The same location of Rf-3 was also found in a population from the cross of IR58025 A//IR36/IR58025 B. At the RG532 locus, different alleles were found between two CMS
lines, Zhenshan 97 A and IR58025 A, and between two restorer lines, IR24 and IR36. The use of these molecular markers closely
linked to Rf-3 in facilitating the development of hybrid rice is discussed.
Received: 3 January 1996 / Accepted: 17 May 1996 相似文献
20.
Sobir T. Ohmori M. Murata F. Motoyoshi 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(1-2):64-69
The Tm-2 gene and its alleles conferring tomato mosaic virus resistance in tomato originate from Lycopersicon peruvianum, a wild relative of tomato. DNA fragments of several RAPD markers tightly linked with the Tm-2 locus in tomato were successfully cloned and sequenced. Subsequently, the 24-mer oligonucleotide primer pairs of the SCAR
markers corresponding to the RAPD markers were designed based on the 5’-endmost sequences. A fragment of the same size as
that of a SCAR marker was amplified in the ToMV-susceptible tomato line with no Tm-2, but the digests of the PCR fragments by AccI exhibited polymorphism in fragment length between the two lines. We chose three SCAR markers and three RAPD markers tightly
linked with the Tm-2 locus, and examined whether the same-sized fragments corresponding to these markers were also present in three other lines
carrying Tm-2a or one of the other Tm-2 alleles. The fragments corresponding to the three SCAR markers were present in all of the three lines, but the other markers
(three RAPDs ) were absent in one or two lines, suggesting that the three SCAR markers are closer to Tm-2 than the other markers. Comparison of the nucleotide sequences of these fragments revealed that they are all homologous to
the corresponding SCAR markers.
Received: 8 November 1999 / Accepted: 15 November 1999 相似文献