Molecular mapping of gene Gm-6(t) which confers resistance against four biotypes of Asian rice gall midge in China |
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Authors: | S K Katiyar Y Tan B Huang G Chandel Y Xu Y Zhang Z Xie J Bennett |
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Institution: | (1) Division of Plant Breeding, Genetics and Biochemistry, International Rice Research Institute, Manila, The Philippines e-mail: j.bennett@cgiar.org Tel.: +63-2-845 0563 x764, Fax: +63-2-845-0606, PH;(2) Indira Gandhi Agricultural University, Raipur, MP, India, IN;(3) Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China, CN;(4) Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China, CN |
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Abstract: | The Chinese rice cultivar Duokang #1 carries a single dominant gene Gm-6(t) that confers resistance to the four biotypes of Asian rice gall midge (Orseolia oryzae Wood-Mason) known in China. Bulked segregant analysis was performed on progeny of a cross between Duokang #1 and the gall
midge-susceptible cultivar Feng Yin Zhan using the RAPD method. The RAPD marker OPM06(1400) amplified a locus linked to Gm-6(t). The locus was subsequently mapped to rice chromosome 4 in a region flanked by cloned RFLP markers RG214 and RG163. Fine
mapping of Gm-6(t) revealed that markers RG214 and RG476 flanked the gene at distances of 1.0 and 2.3 cM, respectively. Another gall midge
resistance gene, Gm-2, mapped previously to chromosome 4, is located about 16 cM from Gm-6(t), to judge by data from a segregating population derived from a cross between Duokang #1 and the Indian cultivar Phalguna
that carries Gm-2. We developed a PCR-based marker-assisted selection kit for transfer of the Gm-6(t) gene into Ming Hui 63 and IR50404, two parental lines commonly used in hybrid rice production in China. The kit contains
PCR primer pairs based on the terminal sequences of the RG214 and RG476 clones. Polymorphism between Duokang #1 and the hybrid
parental lines was found at these markers after digestion of the PCR products with specific restriction endonucleases. The
kit will accelerate introduction of gall midge resistance into hybrid rice in China.
Received: 18 May 2000 / Accepted: 9 March 2001 |
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Keywords: | Rice (Oryza sativa) Gall midge Orseolia oryzae RAPD Mapping Sequence tagged sites |
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