单病种付费方式对县级公立医院住院费用的影响 ——以子宫平滑肌瘤为例

目的 评价安徽省县级公立医院实施单病种付费方式对住院费用的控制效果。方法 采用分层随机抽样方法,抽取子宫平滑肌病例1 314例,其中未采用单病种付费方式的有878例,采用的有436例,进行统计学分析。结果 采用单病种付费方式后人均可比住院费用降低524.254元,住院时间缩短1.13天,药费、护理费、化验费、治疗费和其他费用下降,药费构成比、化验费构成比和治疗费构成比下降(P＜0.05)。结论 单病种付费有利于县级公立医院子宫平滑肌瘤住院费用的控制。     

上海市高血压门诊处方用药合理性研究

目的 调查上海市各级医疗机构的高血压门诊用药合理性情况。方法 采用整群随机抽样的方法选取上海市8家医疗机构,对选定医疗机构2014年5月和11月的高血压门诊处方的合理用药相关指标进行统计分析。结果 平均药品种数为2.24种,基本药物使用率86.43%,平均每张处方基本药物种数1.94种,基药处方占比77.66%,注射剂使用率2.43%,平均药费198.54元。结论 上海市高血压门诊处方总体情况较为合理,二三级医疗机构需注重基本药物的使用,控制高血压门诊处方药费。     

专家门诊亚专科化及专病门诊优化的效果分析

目的 探讨专家门诊亚专科化及专病门诊优化对学科发展及患者就诊的影响。方法 2011年及2014年分别对老年医学科及普通外科专家门诊实行亚专科化并在2013年至2014年对专病门诊进行优化,比较调整前后门诊相关指标的变化。结果 亚专科化后,两个专科的专家门诊量分别增长了63.0%和5.6%,均高于医院同期平均水平（经χ²检验,P=0.000）。普通外科4级手术率达34.9%,高于2013年同期（经χ²检验,P=0.000）,挂号错误率也明显下降（经χ²检验, P=0.000）。2014年底专病门诊增至38个,其中联合专病门诊7个,门诊量增长了36.5%,高于同期专病门诊数量及医院门诊量的增长率（经χ²检验,P=0.000）。结论 专家门诊的亚专科化及充分发挥专病门诊的作用是促进学科建设、提升医院影响力、方便患者就诊的有效措施之一。     

医学影像科检查在医保总额预付改革政策下的变化

目的 探讨医保制对医院影像科检查量的影响以及应对的方法。 方法 对北京大学肿瘤医院医学影像科2012年度全年检查量数据进行统计分析,组间率的比较采用χ²检验或Fisher精确概率法。 结果 医保总额预付制前后放射科检查量总体情况变化不大。非医保群体中,各种检查的构成比情况变化不大。结论 医保总额预付制使医保病例的影像检查手段构成发生了积极变化,减少了病人的医疗支出,促进医疗资源的优化配置;放射科应针对变化采取相应措施,以保证临床医疗和科研工作的平稳开展。     

某专科医院普通门诊分时段预约挂号的探讨

目的 了解某专科医院普通门诊分时段预约的特点和效果,探索适宜的评价指标、评价方式及提升预约服务的有效手段。方法 通过HIS系统提取2015年7月至2016年3月复旦大学附属妇产科医院杨浦院区4个普通门诊参与预约的12 889人次患者的预约信息及挂号就诊的181 447人次患者的相关数据,比较不同门诊的预约情况、流量分布及预约与非预约患者平均候诊时间等的差异。结果 普通门诊预约率仅7.24%,而爽约率高达25.90%;诊间预约不到全部预约的1%,普通门诊预约患者的平均候诊时间普遍短于非预约患者,无论预约率还是预约效果各科室间都存在明显差异。结论 评价普通门诊的预约情况应对各科室设置不同的基线和目标;结合专科医院的特点推动诊间预约;可将门诊流量监控引入门诊预约管理中。     

医院门诊自助服务系统使用率的多因素分析

目的 探索医院门诊一站式自助服务系统在不同干预措施下的使用情况和使用患者的反馈。方法 2015年1—12月通过对门诊进行未干预、3种单独干预各1月、两种较强联合干预和全线干预后,计算自助服务系统的使用人次和使用人次占比,之后对患者进行问卷随访,比较患者的满意度、机器正常运行率和节省的时间。结果 在入口处设置醒目标识提示自助服务系统,多途径宣传一站式服务并且在自助机旁配备充足的指导人员的自助服务系统使用率相对最高。结论 自助服务系统使用率的提升不仅需要多途径、多层次,还需要门诊管理者在必要的时机进行适当的策略调整以取得有效模式来配合门诊运转。

     

北京市某三甲医院31天内非计划性再入院研究

目的 了解北京市某三甲综合性医院住院患者出院31天内非计划再入院的现状及其影响因素。方法 对北京市某三甲综合性医院2008年1月1日—12月31日之间出院后31天内非计划性再入院的患者进行描述性分析，通过t 检验和χ²检验进行单因素分析，选择有统计学意义的危险因素用向后逐步回归法进行非条件Logistic分析。结果 患者性别、患者年龄、出院—再入院的间隔天数、前次入院时入院状况和前次入院疾病是否治愈是31天内非计划性再入院的主要影响因素。结论 患者特征和医院相关因素均与患者出院31天内非计划性再入院相关。     

盈亏平衡分析在医院经营管理中的应用 ——以子长县人民医院为例

目的 探讨陕西省子长县人民医院在实施平价医疗前后经营管理状况的不同,发现存在的问题,并提出相应对策。方法 计算医院2007年和2009年工作量、成本、业务收入,运用盈亏平衡分析计算盈亏平衡点工作量、盈亏平衡点业务收入及安全边际率。结果 通过对医院2年的盈亏平衡分析发现,医院2009年经营状况比2007年好很多,但是,在不考虑财政拨款的情况下,2年均不能实现收支平衡。结论 由结果可见政府的财政拨款对公立医院的经营是至关重要的,另外,医院需从增加业务量及控制成本各方面来保障其经营状况的安全性。     

专家门诊预约挂号影响因素分析

??????? 目的 初步探讨影响专家预约挂号率的原因及相应对策。方法 以SPSS 13.0软件分析2012年4月—2013年1月专家门诊情况与预约挂号率的关系。结果 不同门诊时间（季度、周别、午别）、不同专科、不同专家、不同门诊级别以及挂号率与号源使用率对我院专家门诊预约挂号率均有影响。结论 影响专家门诊预约挂号率的因素是多方面的，需采取综合措施，加强医院核心竞争力才是根本。     

医疗保险费用分析及管理探讨

目的 了解影响医保患者医疗费用的主要因素,为控制医疗费用的不合理增长和医疗保险支付方式的改革提供参考依据。方法 收集2006—2009年三级基本医疗保险定点医疗机构住院费用,对可能的影响因素进行相关分析。结果 人均住院费用呈逐年上涨趋势。次均费用与药品比例、材料呈正相关（决定系数分别为0.914和0.909,P＝0.044和0.046）,P＜0.05。结论 合理降低药费比例和耗材比例是控制医疗费用过快增长的关键。应加快医疗保险支付方式的改革,制定科学的诊疗规范、临床路径。     

壳聚糖酶的克隆表达与壳寡糖的制备分析

目的:克隆壳聚糖酶基因于大肠杆菌中实现高表达,制备壳寡糖。方法:以枯草芽孢杆菌总DNA为模板扩增壳聚糖酶基因(CSN),克隆至载体pET23a(+)上,转化菌株BL21(DE3)。重组子经0.5 mmol/L IPTG诱导后,SDS-PAGE和质谱检测与鉴定重组酶。酶纯化后水解壳聚糖,薄层色谱分析其水解产物。结果:质谱证明壳聚糖酶(31.5kDa)成功表达,表达量占菌体总蛋白的45%左右。纯化后重组酶浓度为900 mg/L,纯度95%、回收率85%,酶活力为10 000 U/mg。壳聚糖降解产物为壳二糖至壳四糖。结论:原核表达载体pET23a(+)-CSN构建正确,壳聚糖酶表达量与活性高,适用于水解壳聚糖制备壳寡糖。     

国内外蝗害治理技术现状与展望

本文首先概述了国内外蝗虫发生与为害的态势,总结了现阶段我国蝗虫发生与为害的主要特点:即农田飞蝗暴发频繁而且严重,草原土蝗的发生时常造成严重的经济损失,而且侵入城市干扰市民生活,我国与周边国家之间蝗虫过境迁移频繁,使用化学农药污染环境和农产品;分析了国内外蝗虫防治对策与技术的发展现状,重点介绍了应急防治和可持续治理对策、...     

Comparison of BMI and percentage of body fat of Indian and German children and adolescents

Today, serious health problems as overweight and obesity are not just constricted to the developed world, but also increase in the developing countries (Prentice 2006, Ramachandram et al. 2002). Focusing on this issue, BMI and percentage of body fat were compared in 2094 schoolchildren from two cross-sectional studies from India and Germany investigated in 2008 and 2009. The German children are in all age groups significantly taller, whereas the Indian children show higher values in BMI (e.g. 12 years: Indian: around 22 kg/m2; German: around 19 kg/m2) and in the percentage of body fat (e.g. 12 years: Indian: around 27%; German: around 18-20%) in most of the investigated age groups. The Indian children have significantly higher BMI between 10 and 13 (boys) respectively 14 years (girls). Indian children showed significant higher percentage of body fat between 10 and 15 years (boys) and between 8 and 16 years (girls). The difference in overweight between Indian and German children was strongest at 11 (boys) and 12 (girls) years: 70% of the Indian but 20% of the German children were classified as overweight. In countries such as India that undergo nutritional transition, a rapid increase in obesity and overweight is observed. In contrast to the industrialized countries, the risk of overweight in developing countries is associated with high socioeconomic status. Other reasons of the rapid increase of overweight in the developing countries caused by different environmental or genetic factors are discussed.     

Synthesis and turnover of cerebrosides and phosphatidylserine of myelin and microsomal fractions of adult and developing rat brain.

The synthesis and turnover of cerebrosides and phospholipids was followed in microsomal and myelin fractions of developing and adult rat brains after an intracerebral injection of [U-14C]serine. The kinetics of incorporation of radioactivity into microsomal and myelin cerebrosides indicate the possibility of a precursor-product relationship between cerebrosides of these membranes. The specific radioactivity of myelin cerebrosides was corrected for the deposition of newly formed cerebrosides in myelin. Multiphasic curves were obtained for the decline in specific radioactivity of myelin and microsomal cerebrosides, suggesting different cerebroside pools in these membranes. The half-life of the fast turning-over pool of cerebrosides of myelin was 7 and 22 days for the developing and adult rat brain respectively. The half-life of the slowly turning-over pool of myelin cerebrosides was about 145 days for both groups of animals. The half-life of the rapidly turning-over microsomal cerebrosides was calculated to be 20 and 40 h for the developing and adult animals respectively. The half-life of the intermediate and slowly turning-over microsomal cerebrosides was 11 and 60 days respectively, for both groups of animals. The amount of incorporation of radioactivity into microsomal cerebrosides from L-serine was greatly decreased in the adult animals, and greater amounts of the precursor were directed towards the synthesis of phosphatidylserine. In the developing animals, considerable amounts of cerebrosides were synthesized from L-serine, besides phosphatidylserine. The time-course of incorporation indicated that a precursor-product relationship exists between microsomal and myelin phosphatidylserine. The half-life of microsomal phosphatidylserine was calculated to be about 8 h for the fast turning-over pool in both groups of animals.     

Identification and composition of the tonsillar and anal enterococcal and streptococcal flora of dogs and cats

Enterococcus faecalis was the most frequently isolated enterococcal species from anal swabs and tonsils of dogs and cats, although in the anal samples from dogs Ent. hirae was found almost as often as Ent. faecalis. Most Ent.faecium strains from dog tonsils differed from those associated with humans and other animals in that they fermented sorbitol. Typical Ent. avium as well as atypical Ent. avium -like strains were seen in dogs, while the related species Ent. raffinosus was associated with cat tonsils. Enterococcus cecorum also occurred mainly in cats. Certain atypical strains, presumptively identified as Ent. cecorum , shared characteristics with Ent. columbae.
The most frequent streptococcal species in tonsils of cats and dogs were Streptococcus suis and Strep. canis. Streptococcus canis and Strep. bovis predominated in anal swabs. The canine Strep. suis differed from the common porcine strains in fermenting mannitol.
Forty-seven of the 288 isolates examined could not be identified or related to known species. The characteristics of two groups of these bacteria, provisionally called 'Ton 31 group' and 'O7 group' are described.     

白术同源四倍体的诱导和鉴定及其与二倍体过氧化物酶的比较

以白术（Atractylodes macrooephala Koidz.）二倍体组培苗为材料,对其四倍体诱导方法进行研究,共获得45个白术同源四倍体株系,为优良株系的选育提供了材料。此外,还分析比较了其中8个白术四倍体株系与二倍体的过氧化物酶同工酶（POD）的酶谱差异,发现四倍体各株系过氧化物酶同工酶谱比二倍体的均多了Rf0.310的谱带,且总过氧化物酶比活力也发生了很大改变,对探讨白术四倍体优良株系的生理生化机理具有一定的参考价值。     

The synthesis and evaluation of flavone and isoflavone chimeras of novobiocin and derrubone

The natural products novobiocin and derrubone have both demonstrated Hsp90 inhibition and structure–activity relationships have been established for each scaffold. Given these compounds share several key structural features, we hypothesized that incorporation of elements from each could provide insight to structural features important for Hsp90 inhibition. Thus, chimeric analogues of novobiocin and derrubone were constructed and evaluated. These studies confirmed that the functionality present at the 3-position of the isoflavone plays a critical role in determining Hsp90 inhibition and suggests that the bicyclic ring system present in both novobiocin and derrubone do not share similar modes of binding.