综合医院变频工作制管理模式的构想

在综合医院人力资源配置和工作方式现状分析的基础上,对相关理论进行了系统回顾分析和比较;阐述了饱和工作制、弹性工作制和变频工作制模式的优缺点;提出变频工作制是人力资源的一种动态管理机制,是弹性工作制的进一步发展,能更加充分利用员工的工作时间,提高工作效率,使资源合理地优化配置。     

主辅分离下大型公立医院创新发展运行模式探索

创新作为现代医院发展的支点，是医院科学运营的动力。企业医院主辅分离后，通过观念创新、技术创新、服务创新和整体发展规模的精心规划，在决策方向、经营运作、核心能力、整体规模上的重点把握和持续创新，提升了医院科学发展力、市场适应力和持续创新力，实现了医院的跨跃式发展。     

引入手术危险评分细化外科手术分级准入的探索

分析了当前外科手术权限管理方法和存在的问题。作为心血管病专科医院，尝试引入外科术前危险评分，对手术进行分级，建立手术准入控制系统和手术医师档案系统，全面记录医师外科手术情况，为评价医师技术能力和科学地审核医师手术权限申请提供了量化依据。     

基于能级管理模式的护士“三定”管理与评价

尝试以“定员管理、定向培养、定能分级”为核心的新型护理能级管理模式，改善管理者和被管理者的人际关系，加快专科护士培养进程，提升护士职业发展空间，深化护理内涵质量，推进优质护理服务。     

门诊患者医疗服务感受度影响因素研究

??????? 目的 了解门诊患者对就医服务感受度影响因素,以期提高患者满意度。 方法 现场问卷调查法、专家访谈法。 结果 患者在门诊过程中最为关注的就诊流程、等候时间、就医环境、医德医风四个因素,非常看重医护人员服务规范,服务流程便捷流畅。病情不同患者对等候时间要求不同,对医院熟悉程度与就诊环境感受度不同。结论 在门诊服务中为患者提供便捷流畅、规范化的服务,同时应注重患者的不同服务需求。     

医院院长职业化概念的探讨

医院院长职业化随同新医改“管办分离、法人治理”精神的出台再度引起业内的广泛关注,但何为院长职业化、何为职业院长等基本的概念仍尚未厘清,难免导致研究和实践的迷误。因而对现存代表性的模糊认识进行剖析,并结合国内外相关概况研究评述,最后得出中国特色的院长职业化的概念,以期为相关研究和实践提供参考。     

居民健康卡金融功能应用模式探讨

阐述了居民健康卡金融应用意义,分析了居民健康卡实现金融功能的相关技术基础,探讨了居民健康卡加载银行账户、银行卡绑定居民健康卡、居民健康卡动态绑定银行卡加储值卡等金融功能实现方法及相关应用。     

基于Malmquist指数的我国省际医院生产效率分析

目的 分析我国医院效率的动态变化及来源。方法 利用马奎斯特指数及分解。结果 2010—2014年间我国医院的马奎斯特指数呈上升趋势。结论 目前我国医院的全要素生产率改善主要来自纯技术效率的变化,说明我国医院逐步从以前粗放式发展阶段迈向精耕细作的新阶段,规模效率大于1,表明组建医药集团经营的可行性。     

应用数据包络分析方法评价医院临床科室相对效率的探讨

目的 利用数据包络分析方法评价厦门市某三级综合性医院临床科室的配置效率，为医院管理者提供有益的决策信息。方法 在Excel的规划求解模块上进行数据包络分析计算，得出各临床科室的相对效率得分。结果 数据包络分析有效单元为15个，其余单元不同程度存在产出不足的现象。结论 大型综合性医院在自身的生存和发展中，应注重以资源调整和优化配置为主的内涵式发展。     

大型综合性医院协同管理模式研究

管理信息化对提高医院管理水平有重要意义。直线式管理结构和复杂行政流程,与当前医院快速发展有许多不适应之处。文章阐述了如何利用信息技术在医院管理中引入协同的理念,通过公开、分析和挖掘有用信息,加强信息的反馈与再利用、建立信息流通的环路,实现人、数据、和流程的协调运作,从而最终实现医院透明化与精细化管理,提高工作效率,打造坚实的医院品牌。     

壳聚糖酶的克隆表达与壳寡糖的制备分析

目的:克隆壳聚糖酶基因于大肠杆菌中实现高表达,制备壳寡糖。方法:以枯草芽孢杆菌总DNA为模板扩增壳聚糖酶基因(CSN),克隆至载体pET23a(+)上,转化菌株BL21(DE3)。重组子经0.5 mmol/L IPTG诱导后,SDS-PAGE和质谱检测与鉴定重组酶。酶纯化后水解壳聚糖,薄层色谱分析其水解产物。结果:质谱证明壳聚糖酶(31.5kDa)成功表达,表达量占菌体总蛋白的45%左右。纯化后重组酶浓度为900 mg/L,纯度95%、回收率85%,酶活力为10 000 U/mg。壳聚糖降解产物为壳二糖至壳四糖。结论:原核表达载体pET23a(+)-CSN构建正确,壳聚糖酶表达量与活性高,适用于水解壳聚糖制备壳寡糖。     

国内外蝗害治理技术现状与展望

本文首先概述了国内外蝗虫发生与为害的态势,总结了现阶段我国蝗虫发生与为害的主要特点:即农田飞蝗暴发频繁而且严重,草原土蝗的发生时常造成严重的经济损失,而且侵入城市干扰市民生活,我国与周边国家之间蝗虫过境迁移频繁,使用化学农药污染环境和农产品;分析了国内外蝗虫防治对策与技术的发展现状,重点介绍了应急防治和可持续治理对策、...     

Coiled-coil nanomechanics and uncoiling and unfolding of the superhelix and alpha-helices of myosin

The nanomechanical properties of the coiled-coils of myosin are fundamentally important in understanding muscle assembly and contraction. Force spectra of single molecules of double-headed myosin, single-headed myosin, and coiled-coil tail fragments were acquired with an atomic force microscope and displayed characteristic triphasic force-distance responses to stretch: a rise phase (R) and a plateau phase (P) and an exponential phase (E). The R and P phases arise mainly from the stretching of the coiled-coils, with the hinge region being the main contributor to the rise phase at low force. Only the E phase was analyzable by the worm-like chain model of polymer elasticity. Restrained molecular mechanics simulations on an existing x-ray structure of scallop S2 yielded force spectra with either two or three phases, depending on the mode of stretch. It revealed that coiled-coil chains separate completely near the end of the P phase and the stretching of the unfolded chains gives rise to the E phase. Extensive conformational searching yielded a P phase force near 40 pN that agreed well with the experimental value. We suggest that the flexible and elastic S2 region, particularly the hinge region, may undergo force-induced unfolding and extend reversibly during actomyosin powerstroke.     

放牧对牧草光合作用、呼吸作用和氮、碳吸收与转运的影响

研究放牧对草地植物生理活动的影响,对于揭示草地放牧演替的生理机制有重要意义.大量研究表明,家畜放牧对牧草光合作用、呼吸作用以及C和N吸收与转运的影响,可以分为生理伤害和生理恢复2个阶段.放牧通过改变草地冠层结构影响牧草光合作用,净光合作用速率短期内迅速下降,随着叶面积指数增加又逐渐上升,呼吸作用有相似的变化趋势.牧草放牧后再生长所需的C和N最初主要来自根系和留茬中的贮藏物质,此后随着牧草生长恢复逐渐由同化作用供给,C代谢与土壤N水平负相关.放牧后牧草生理活动变化与牧草遗传特性、种间竞争、家畜放牧特征、非生物环境等因素密切相关.     

Kinetics and thermodynamics of peroxidase- and laccase-catalyzed oxidation of N-substituted phenothiazines and phenoxazines

N -substituted phenothiazines (PTs) and phenoxazines (POs) catalyzed by fungal Coprinus cinereus peroxidase and Polyporus pinsitus laccase were investigated at pH 4–10. In the case of peroxidase, an apparent bimolecular rate constant (expressed as k _cat/K _m) varied from 1 ×10⁷M⁻¹s⁻¹to 2.6×10⁸M⁻¹s⁻¹ at pH 7.0. The constants for PO oxidation were higher in comparison to PT. pH dependence revealed two or three ionizable groups with pK _a values of 4.9–5.7 and 7.7–9.7 that significantly affected the activity of peroxidase. Single-turnover experiments showed that the limiting step of PT oxidation was reduction of compound II and second-order rate constants were obtained which were consistent with the constants at steady-state conditions. Laccase-catalyzed PT and PO oxidation rates were lower; apparent bimolecular rate constants varied from 1.8×10⁵M⁻¹s⁻¹ to 2.0×10⁷M⁻¹s⁻¹ at pH 5.3. PO constants were higher in comparison to PT, as was the case with peroxidase. The dependence of the apparent bimolecular constants of compound II or copper type 1 reduction, in the case of peroxidase or laccase, respectively, was analyzed in the framework of the Marcus outer-sphere electron-transfer theory. Peroxidase-catalyzed reactions with PT, as well as PO, fitted the same hyperbolic dependence with a maximal oxidation rate of 1.6×10⁸M⁻¹s⁻¹ and a reorganization energy of 0.30 eV. The respective parameters for laccase were 5.0×10⁷M⁻¹s⁻¹ and 0.29 eV. Received: 20 September 1999 / Accepted: 24 February 2000     

Synthesis of hapten and conjugates of coumestrol and development of immunoassay

3-O-Carboxymethylcoumestrol was prepared as the hapten for immunoassay by a partial alkylation of coumestrol with ethyl chloroacetate in acetone alkalized with potassium carbonate. 3-O-Ethoxycarbonylmethylcoumestrol was separated by column chromatography and finally was hydrolyzed with formic acid. 1H and 13C NMR data (APT, COSY, HMQC, and HMBC) revealed that the reaction was regioselective, as 3-O-ethoxycarboxymethylcoumestrol was the only monosubstituted derivative. The hapten was then conjugated to bovine serum albumin and used for immunization of rabbits. A radioimmunoassay (RIA) system was established based on the polyclonal antiserum and a 125I-labeled hapten-tyrosine methyl ester conjugate as the radioligand. Parameters of the RIA: sensitivity: 12 pg per tube, 50% intercept: 140 pg per tube, working range: 20-4000 pg per tube. The cross-reactivity of a panel isoflavonoid and lignan phytoestrogens was either negligible (e.g. formononetin 0.07%; biochanin A 0.06%) or not detectable at all. The major immunoreactive peak in HPLC fractions from an alfalfa extract had the same retention time as coumestrol standard and represented 94.8% of the signal. The remaining 5.2% of immunoreactivity was distributed between five minor peaks. We conclude that after the validation for particular matrices, the method will be a useful tool for analysis of coumestrol, especially in low volume and low concentration samples.     

白术同源四倍体的诱导和鉴定及其与二倍体过氧化物酶的比较

以白术（Atractylodes macrooephala Koidz.）二倍体组培苗为材料,对其四倍体诱导方法进行研究,共获得45个白术同源四倍体株系,为优良株系的选育提供了材料。此外,还分析比较了其中8个白术四倍体株系与二倍体的过氧化物酶同工酶（POD）的酶谱差异,发现四倍体各株系过氧化物酶同工酶谱比二倍体的均多了Rf0.310的谱带,且总过氧化物酶比活力也发生了很大改变,对探讨白术四倍体优良株系的生理生化机理具有一定的参考价值。