中国隆痣短柄泥蜂属研究及二新记录种记述（膜翅目：方头泥蜂科）

记述我国已知的隆痣短柄泥蜂属Carinostigmus Tsuneki 9种,其中包括2中国新记录种:脉隆痣短柄泥蜂Carinostigmus costatus Krombein, 1984,以及弯柄隆痣短柄泥蜂Carinostigmus maior (Maidl, 1925)。编制了中国隆痣短柄泥蜂属已知种类检索表。     

西双版纳不同经济作物环境泥蜂物种多样性研究

泥蜂是一类重要的捕猎性天敌昆虫和传粉昆虫。本研究于2018年6月-2019年11月对西双版纳地区观赏经作区、柚林区、古茶林区和农田区（样地Ⅰ-Ⅳ）4个调查区采用马氏网诱捕方法定期定点调查,每月调查一次。结果如下：西双版纳调查区域分布有泥蜂类群3科15族27属110种;其中,泥蜂科包括3族5属7种;蠊泥蜂科包括2族3属8种;方头泥蜂科包括10族19属95种,为该地区的优势科。脊短柄泥蜂属Psenulus、小唇泥蜂属Larra和短翅泥蜂属Trypoxylon为优势属,刻臀小唇泥蜂Larra fenchihuensis、磨光小唇泥蜂红腿亚种Larra polita luzonensis为优势种。4个作物区的优势科均为方头泥蜂科,但不同作物区泥蜂的优势属种存在差异,观赏经作区（样地Ⅰ）优势属为脊短柄泥蜂属和短翅泥蜂属,优势种为普氏脊短柄泥蜂指名亚种Psenulus pulcherrimus pulcherrimus（17.4%）和岩田隆痣短柄泥蜂Carinostigmus iwatai（15.6%）;柚木林区（样地Ⅱ）优势属为脊短柄泥蜂属和短翅泥蜂属,优势种为普氏脊短柄泥蜂指名亚种（17.7%）和锡兰脊短柄泥蜂Psenulus ceylonicus（11.3%）;古茶林区（样地Ⅲ）优势属为短翅泥蜂属和小唇泥蜂属,优势种为刻臀小唇泥蜂（15.6%）和吉打州短翅泥蜂Trypoxylon kedah（12.5%）;农田区（样地Ⅳ）优势属为小唇泥蜂属和短翅泥蜂属,优势种为刻臀小唇泥蜂（21.7%）和磨光小唇泥蜂红腿亚种（21.7%）。15属多区分布,12属单区分布。植被差异是影响泥蜂物种多样性的重要因子,植被群落复杂程度影响泥蜂群落特征指数,植被群落越复杂的环境,泥蜂群落种类越丰富,物种多样性越高。泥蜂物种多样性随季节的变化而明显变化,降雨量和温度等气候因素是影响泥蜂多样性变化的重要因子。4个样地之间泥蜂群落相似性系数表示为中等不相似或极度不相似水平,表明不同样地中泥蜂群落组成差异很大。本研究明确了西双版纳4个不同经济作物环境中的泥蜂物种多样性,可为天敌昆虫资源的保护和利用以及害虫生物防治等研究提供依据。     

中国转长泥蜂属一新种记述(膜翅目:方头泥蜂科)

记述中国浙江方头泥蜂科Crabronidae转长泥蜂属Tracheliodes 1新种:瘤唇转长泥蜂Tracheliodes labitubercutus,sp.nov.,编制了该属中国已知种类检索表.模式标本保存于浙江大学寄生蜂标本室.     

短柄泥蜂亚科中国新记录亚族、属及种类记述(膜翅目:方头泥蜂科)

记述短柄泥蜂亚科1中国新记录亚族(圆痣短柄泥蜂亚族Ammoplanina Evans)、2中国新记录属(锐痣短柄泥蜂属Ammoplanops Gussakovskij和圆痣短柄泥蜂属Ammoplanus Giraud)及5中国新记录种(脊锐痣短柄泥蜂Ammoplanops carinatus Gussakovskij,...     

中国全脉茧蜂属一新种（膜翅目： 茧蜂科）

记述了窄径茧蜂亚科Agathidinae的中国大陆一新记录属-全脉茧蜂属Earinus Wesmael及该属已知分布于福建省武夷山的一新种-武夷全脉茧蜂Earinus wuyiensis Chen et Yang, 并建立了该属中国已知3种的分种检索表。新种模式标本保存于福建农业大学植物保护系益虫研究室。     

中国棒柄泥蜂属二新种记述 (膜翅目：泥蜂科)

该文记述了采自我国西南部及甘肃省的泥蜂科方头泥蜂亚科棒柄泥蜂属2新种：角唇棒柄泥蜂Rhopalum(Rhopalum)cornilabiatum,spnov和甘肃棒柄泥蜂Rhopalum(Rhopalum)gansuense,spnov模式标本分别保存在浙江农业大学昆虫标本室和中国科学院动物研究所昆虫标本馆。     

沙泥蜂属一新种记述(膜翅目 :泥蜂科)(英文)

记述了采自我国新疆的沙泥蜂属 1新种 ,伪海沙泥蜂AmmophilapseudoheydeniLietHe ,sp nov ,新种与A heydeniDahlbom相似 ,但唇基前缘形状、盾片和并胸腹节背区的皱纹、腹柄明显长于后足跗节Ⅰ +Ⅱ长度、雄性腹柄仅短于后足胫节长的 1/ 5～ 1/ 6以及体色和雄性外生殖器等特征明显有别。正模♀ ,副模4♀♀ ,8♂♂ ,均采自我国新疆。模式标本分别保存在浙江农业大学昆虫标本室和中国科学院动物研究所昆虫标本馆     

中国三室短柄泥蜂属一新种记述(膜翅目:方头泥蜂科)

记述方头泥蜂科三室短柄泥蜂属1新种——排刺三室短柄泥蜂Psenseriatispinosus,sp.nov.。模式标本保存于浙江大学昆虫标本室。     

基于28S rRNA D2序列的内茧蜂亚科的分子系统发育

首次利用同源28S rRNA D2基因序列对内茧蜂亚科Rogadinae （昆虫纲Insecta：膜翅目Hymenoptera：茧蜂科Braconidae）进行了分子系统学研究。本研究从95%～100%乙醇浸渍保存的标本中提取基因组DNA并扩增了10种内群种类和5种外群种类的28S rDNA D2片段并测序（GenBank序列号AY167645-AY167659）,利用BLAST搜索相关的同源序列, 采用了GenBank中13个种类的28S rRNA D2同源序列,然后据此进行分子分析。利用3个外群（共8个种类）和3种建树方法 (距离邻近法distance based neighbor joining, NJ; 最大俭约法maximum parsimony, MP; 和最大似然法maximum likelihood, ML)分析了内茧蜂亚科内的分子系统发育关系。结果表明,由分子数据产生的不同的分子系统树均显示内茧蜂亚科是一个单系群。内茧蜂亚科内依据形态和生物学特征的分群（族和亚族）及其系统发育关系得到部分支持。NJ、MP和ML分析结果均表明内茧蜂族Rogadini不是一个单系,而是一个并系,其余3族则得到不同程度的支持。内茧蜂族可分成2个分支：“脊茧蜂属Aleiodes+弓脉茧蜂属Arcaleiodes”和“沟内茧蜂属Canalirogas+锥齿茧蜂属Conspinaria+刺茧蜂属Spinaria+内茧蜂属Rogas”,二者不是姐妹群。脊茧蜂属Aleiodes和弓脉茧蜂属Arcaleiodes始终是姐妹群。脊茧蜂属Aleiodes是一个单系,并可分成2个姐妹分支,这与依据形态和生物学特征的亚属分群相一致。弓脉茧蜂属Arcaleiodes Chen et He,1991是一个独立的属。分支“沟内茧蜂属Canalirogas+锥齿茧蜂属Conspinaria+刺茧蜂属Spinaria+内茧蜂属Rogas”的单系性仅得到部分分子数据的支持;因形态特异（腹部成甲壳状）而列为亚族级的刺茧蜂属Spinaria,分子分析没有证实这一点。横纹茧蜂族Clinocentrini是个单系,并在内茧蜂亚科的系统发育中处于基部（原始）的位置。我们研究结果还表明,阔跗茧蜂属Yelicones和潜蛾茧蜂属Stiropius相对应的阔跗茧蜂族Yeliconini和潜蛾茧蜂族Stiropiini为2个独立的分支, 与形态和生物学的结果一致,但它们在内茧蜂亚科的系统发育的位置不明,有待今后进一步研究。     

新疆米短柄泥蜂属二新种记述(膜翅目,蜜蜂总科,方头泥蜂科)(英文)

记述了采自新疆2新种,四齿米短柄泥蜂M.quadridentata Ma,Liet Chen,sp.nov.和散点米短柄泥蜂M.sparsipunctulata Ma,Liet Chen,sp.nov.,对米短柄泥蜂属中国已知种类检索表进行了部分修订。模式标本分别保存在浙江大学寄生蜂标本室和中国科学院动物研究所昆虫标本馆。     

Characterization of heparin oligosaccharides binding specifically to antithrombin III using mass spectrometry

Heparan sulfate (HS) is a sulfated glycosaminoglycan attached to a core protein on the cell surface. Protein binding to cell surface HS is a key regulatory event for many cellular processes such as blood coagulation, cell proliferation, and migration. The concept whereby protein binding to HS is not random but requires a limited number of sulfation patterns is becoming clear. Here we describe a hydrophobic trapping assay for screening a library of heparin hexasaccharides for binders to antithrombin III (ATIII). The hexasaccharide compositions are defined with their building block content in the following format: (DeltaHexA:HexA:GlcN:SO 3:Ac). Of five initial compositions present in the library, (1:2:3:6:1), (1:2:3:7:1), (1:2:3:7:0), (1:2:3:8:0), and (1:2:3:9:0), only two are shown to bind ATIII, namely, (1:2:3:8:0) and (1:2:3:9:0). The use of amide hydrophilic interaction (HILIC) liquid chromatography-mass spectrometry permitted reproducible quantitative analysis of the composition of the initial library as well as that of the binding fraction. The specificity of the hexasaccharides binding ATIII was confirmed by assaying their ability to enhance ATIII-mediated inhibition of Factor Xa in vitro.     

The Zellweger syndrome: deficient conversion of docosahexaenoic acid (22:6(n-3)) to eicosapentaenoic acid (20:5(n-3)) and normal delta 4-desaturase activity in cultured skin fibroblasts

The metabolism of docosahexaenoic acid (22:6(n-3)) and adrenic acid (22:4(n-6)) was studied in cultured fibroblasts from patients with the Zellweger syndrome, X-linked adrenoleukodystrophy (X-ALD) and normal controls. It was shown that [4,5- 3H]22:6(n-3) is retroconverted to labelled eicosapentaenoic acid (20:5(n-3)) in normal and X-ALD fibroblasts, while this conversion is deficient in Zellweger fibroblasts. [U- 14C]Eicosapentaenoic acid (20:5(n-3)) is elongated to docosapentaenoic acid (22:5(n-3)) in all three cell lines. With [U- 14C]20:5(n-3) as the substrate, shorter fatty acids were not detected. With [4,5- 3H]22:6(n-3) as the substrate, labelled fatty acids were esterified in the phospholipid- and triacylglycerol-fraction to approximately the same extent in all three cell lines. [2- 14C]Adrenic acid (22:4(n-6)) was desaturated to 22:5(n-6) and elongated to 24:4(n-6) in all three cell lines and to the largest extent in the Zellweger fibroblasts. This agrees with the view that the delta 4-desaturase is not a peroxisomal enzyme. The observation that the retroconversion of 22:6(n-3) to 20:5(n-3) is deficient in Zellweger fibroblasts strongly suggest that the beta-oxidation step in the retroconversion is a peroxisomal function. Peroxisomal very-long-chain (lignoceroyl) CoA ligase is probably not required for the activation of 22:6(n-3), since the retroconversion to 20:5(n-3) is normal in X-ALD fibroblasts.     

A general method for the quantitative analysis of functional chimeras: applications from site-directed mutagenesis and macromolecular association

Two new parameters, I: and C:, are introduced for the quantitative evaluation of functional chimeras: I: (impact) and C: (context dependence) are the free energy difference and sum, respectively, of the effects on a given property measured in forward and retro chimeras. The forward chimera is made by substitution of a part "a" from ensemble A into the analogous position of homologous ensemble B (S:(B --> A)). The C: value is a measure of the interaction of the interrogated position with its surroundings, whereas I: is an expression of the quantitative importance of the probed position. Both I: and C: vary with the evaluated property, for example, kinetics, binding, thermostability, and so forth. The retro chimera is the reverse substitution of the analogous part "b" from B into A, S:(A --> B). The I: and C: values derived from original data for forward and retro mutations in aspartate and tyrosine aminotransferase, from literature data for quasi domain exchange in oncomodulin and for the interaction of Tat with bovine and human TAR are evaluated. The most salient derived conclusions are, first, that Thr 109 (AATase) or Ser 109 (TATase) is an important discriminator for dicarboxylic acid selectivity by these two enzymes (I: < -2.9 kcal/mol). The T109S mutation in AATase produces a nearly equal and opposite effect to S109T in TATase (C: < 0.4 kcal/mol). Second, an I: value of 5.5 kcal/mol describes the effects of mirror mutations D94S (site 1) and S55D (site 2) in the Ca(2+) binding sites of oncomodulin on Ca(2+) affinity. The second mirror set, G98D (site 1) and D59G (site 2), yields a smaller impact (I: = -3.4 kcal/mol) on Ca(2+) binding; however, the effect is significantly more nearly context independent (C: = -0.6 versus C: = -2.7 kcal/mol). Third, the stem and loop regions of HIV and BIV TAR are predominantly responsible for the species specific interaction with BIV Tat(65-81) (I: = -1.5 to -1.6 kcal/mol), whereas I: = 0.1 kcal/mol for bulge TAR chimeras. The C: values are from -0.3 to -1.2 kcal/mol. The analysis described should have important applications to protein design.     

Substrate selectivity of plant and microbial lysophosphatidic acid acyltransferases

Linoleic acid (18:2) is found in a large variety of plant oils but to date there is limited knowledge about the substrate selectivity of acyltransferases required for its incorporation into storage triacylglycerols. We have compared the incorporation of oleoyl (18:1) and linoleoyl (18:2) acyl-CoAs onto lysophosphatidic acid acceptors by sub-cellular fractions prepared from a variety of plant and microbial species. Our assays demonstrated: (1). All lysophosphatidic acid acyltransferase (LPA-AT) enzymes tested incorporated 18:2 acyl groups when presented with an equimolar mix of 18:1 and 18:2 acyl-CoA substrates. The ratio of 18:1 to 18:2 incorporation into phosphatidic acid varied between 0.4 and 1.4, indicating low selectivity between these substrates. (2). The presence of either stearoyl (18:0) or oleoyl (18:1) groups at the sn-1 position of lysophosphatidic acid did not affect the selectivity of incorporation of 18:1 or 18:2 into the sn-2 position of phosphatidic acid. (3). All LPA-AT enzymes tested incorporated the saturated palmitoyl (16:0) acyl group from equimolar mixtures of 16:0- and 18:1-CoA. The ratios of 18:1 to 16:0 incorporation are generally much higher than those of 18:1 to 18:2 incorporation, varying between 2.1 and 8.6. (4). The LPA-AT from oil palm kernel is an exception as 18:1 and 16:0 are utilised at comparable rates. These results show that, in the majority of species examined, there is no correlation between the final sn-2 composition of oil or membrane lipids and the ability of an LPA-AT to use 18:2 as a substrate in in vitro assays.     

Conformational selection by the aIF2 GTPase: a molecular dynamics study of functional pathways

Archaeal initiation factor 2 (aIF2) is a GTPase involved in protein biosynthesis. In its GTP-bound, "ON" conformation, it binds an initiator tRNA and carries it to the ribosome. In its GDP-bound, "OFF" conformation, it dissociates from tRNA. To improve our understanding of the role of each conformational state in the aIF2 "life cycle", we start from the state immediately after GTP hydrolysis, ON:GDP:P(i) (where P(i) is inorganic phosphate), and consider the possible next steps on the pathway to the OFF:GDP product. The first possibility is P(i) dissociation, leading to ON:GDP, which could then relax into OFF:GDP. We use molecular dynamics simulations to compute the P(i) dissociation free energy and show that dissociation is highly favorable. The second possibility is conformational relaxation into the OFF state before P(i) dissociation, to form OFF:GDP:P(i). We estimate the corresponding free energy approximately, 2 ± 3.5 kcal/mol, so that this is an uphill or weakly downhill process. A third possibility is relaxation into another conformation, neither ON nor OFF. Indeed, a third, "MIXED" conformation was seen recently in a crystal structure of the aIF2:GDP:P(i) complex. For this conformational state, P(i) dissociation is weakly unfavorable, in contrast to the ON and OFF states. From this, we will deduce that if the MIXED:GDP complex is not too unstable, the ON:GDP:P(i) → MIXED:GDP:P(i) transformation is a downhill process, which can occur spontaneously. This suggests that the MIXED state could be a functional intermediate.     

A taxonomic study on semifumata species-group of Fissocantharis Pic, with description of six new species from China and Myanmar (Coleoptera, Cantharidae)

The cantharid Fissocantharis semifumata species-groupis reviewed. Fissocantharis semifumata (Fairmaire, 1889) is redescribed and illustrated. The type series of Fissocantharis fissa (Wittmer, 1997) is shown to consist of 3 species and clarified, except the holotype, the two paratypes become invalid. Fissocantharis grahami (Wittmer, 1997) is attributed to this species group. Six new species are described and illustrated, Fissocanthais yuisp. n. (CHINA: Yunnan), Fissocantharis semimetallicasp. n. (CHINA: Yunnan; MYANMAR: Kachin), Fissocantharis bicoloratasp. n. (CHINA: Sichuan), Fissocantharis maculicepssp. n. (CHINA: Gansu), Fissocantharis bimaculatasp. n. (CHINA: Sichuan) and Fissocantharis flavasp. n. (CHINA: Sichuan, Guizhou). The number of species in the Fissocantharis semifumata species-groupis increased from 4 to 11, and a key to all species is provided.     

Comparison of bovine serum transferrin A and D2. I. Amino acid residue differences

A comparison is made of single components of the homozygous variants A and D2 of bovine serum transferrin by tryptic, chymotryptic and cyanogen bromide digestion. It is concluded that there are three substitutions A:D2-Glu:Asp, Lys:Arg and Asp:Gly. In the light of the recent work of Brock et al. (1980) it is concluded that all three substitutions occur in the C-terminal sequence of the chain. By homology with the sequence of human serum transferrin (MacGillivray et al., 1982) the Lys:Arg and Asp:Gly substitutions probably occur at residues 527 and 446, respectively, from the N-terminus. The Asp:Gly substitution is considered more likely than our earlier conclusion (Maeda, McKenzie & Shaw, 1977) that there is a deletion in the chain of D2 (A:D2, Asp:--). The location of the Glu:Asp substitution is not known.     

Comparison of bovine serum transferrin A and D2. I. Amino acid residue differences

A comparison is made of single components of the homozygous variants A and D₂ of bovine serum transferrin by tryptic, chymotryptic and cyanogen bromide digestion. It is concluded that there are three substitutions A:D₂ - Glu:Asp, Lys: Arg and Asp:Gly. In the light of the recent work of Brocket al. (1980) it is concluded that all three substitutions occur in the C-terminal sequence of the chain. By homology with the sequence of human serum transferrin (MacGillivray et al., 1982) the Lys:Arg and Asp:Gly substitutions probably occur at residues 527 and 446, respectively, from the N -terminus. The Asp:Gly substitution is considered more likely than our earlier conclusion (Maeda, McKenzie & Shaw, 1977) that there is a deletion in the chain of D₂ (A:D₂, Asp: —). The location of the Glu:Asp substitution is not known.     

Molecular organization of cholesterol in polyunsaturated phospholipid membranes: a solid state 2H NMR investigation.

We compared the molecular organization of equimolar [3alpha-2H1]cholesterol in 18:0-18:1PC (1-stearoyl-2-oleoylphosphatidylcholine), 18:0-22:6PC (1-stearoyl-2-docosahexaenoylphosphatidylcholine), 18:0-20:4PC (1-stearoyl-2-arachidonylphosphatidylcholine) and 20:4-20:4PC (1,2-diarachidonylphosphatidylcholine) bilayers by solid state 2H NMR. Essentially identical quadrupolar splittings (delta v(r) = 45 +/- 1 kHz) corresponding to the same molecular orientation characterized by tilt angle alpha0 = 16 +/- 1 degrees were measured in 18:0-18:1PC, 18:0-22:6PC and 18:0-20:4PC. A profound difference in molecular interaction with dipolyunsaturated 20:4-20:4PC, in contrast, is indicated for the sterol. Specifically, the tilt angle alpha0 = 22 +/- 1 degrees (derived from delta v(r) = 37 +/- 1 kHz) is greater and its membrane intercalation is only 15 mol%.     

Poly (I:C), an agonist of toll-like receptor-3, inhibits replication of the Chikungunya virus in BEAS-2B cells

ABSTRACT: BACKGROUND: Double-stranded RNA (dsRNA) and its mimic, polyinosinic acid: polycytidylic acid [Poly (I:C)], are recognized by toll-like receptor 3 (TLR3) and induce interferon (IFN)-beta in many cell types. Poly (I:C) is the most potent IFN inducer. In in vivo mouse studies, intraperitoneal injection of Poly (I:C) elicited IFN-alpha/beta production and natural killer (NK) cells activation. The TLR3 pathway is suggested to contribute to innate immune responses against many viruses, including influenza virus, respiratory syncytial virus, herpes simplex virus 2, and murine cytomegalovirus. In Chikungunya virus (CHIKV) infection, the viruses are cleared within 7-10 days postinfection before adaptive immune responses emerge. The innate immune response is important for CHIKV clearance. RESULTS: The effects of Poly (I:C) on the replication of CHIKV in human bronchial epithelial cells, BEAS-2B, were studied. Poly (I:C) suppressed cytopathic effects (CPE) induced by CHIKV infection in BEAS-2B cells in the presence of Poly (I:C) and inhibited the replication of CHIKV in the cells. The virus titers of Poly (I:C)-treated cells were much lower compared with those of untreated cells. CHIKV infection and Poly (I:C) treatment of BEAS-2B cells induced the production of IFN-beta and increased the expression of anti-viral genes, including IFN-alpha, IFN-beta, MxA, and OAS. Both Poly (I:C) and CHIKV infection upregulate the expression of TLR3 in BEAS-2B cells. CONCLUSIONS: CHIKV is sensitive to innate immune response induced by Poly (I:C). The inhibition of CHIKV replication by Poly (I:C) may be through the induction of TLR3, which triggers the production of IFNs and other anti-viral genes. The innate immune response is important to clear CHIKV in infected cells.