乙型肝炎病毒X蛋白上调环加氧酶2表达促进肝癌细胞增殖的实验研究

乙肝病毒X蛋白（Hepatitis B virus X protein,HBx）高表达与乙肝相关肝癌的发病密切相关,但HBx发挥促癌作用的机制并不清楚。环加氧酶2（Cyclooxygenase,COX2）具有促进肝癌细胞增殖的功能,乙肝相关肝癌中COX2的表达增加且与HBx呈正相关,提示上调COX2可能是HBx促进肝癌细胞增殖的分子机制。为了阐明HBx是否通过上调COX2促进肝癌细胞增殖,本实验培养了肝癌HepG2细胞并分为对照组、转染pcDNA3.1-HBx质粒的HBx组、转染NC siRNA的si-NC组、转染NC siRNA及pcDNA3.1-HBx质粒的si-NC+HBx组、转染COX2 siRNA及pcDNA3.1-HBx质粒的si-COX2+HBx组。检测细胞增殖活力OD_490nm的水平,COX2、B淋巴细胞瘤2基因（BCL2）、生存素（Survivin）的表达量,前列腺素E2（英文名,PGE2）的含量。实验结果显示,HBx组的OD_490nm水平,细胞中COX2、BCL2、Survivin的表达量,培养基中PGE2的含量均高于对照...     

膜翅目昆虫干标本的基因组DNA提取

提出了膜翅目昆虫干标本基因组DNA的提取方法,通过对实验结果和所测得的基因片段（28S D2 rDNA)的分析,表明该方法可行。     

在韩国危害红松球果的三种蛾类幼虫形态记述（鳞翅目）

详细地记述了韩国红松球果害虫冷杉梢斑螟Dioryctria abietella、赤松梢斑螟D. sylvestrella (螟蛾科)和小花尺蛾Eupithecia abietaria debrunneata (尺蛾科)幼虫的形态特征,并提供了形态特征图。     

韩国三种枝尺蛾亚科老熟幼虫的形态记述(鳞翅目:尺蛾科)(英文)

详细地记述了枝尺蛾亚科Petelia rivulosa (Butler)、Exangerona prattiaria(Leech)及Culcula panterinaria(Bremer＆Grey)老熟幼虫的形态特征,并提供了形态特征图。所有标本均保存在韩国江原大学校森林资源保护学科昆虫标本室。     

中国四种扇野螟属幼虫形态记述及生物学习性（鳞翅目：草螟科：斑野螟亚科）

记述中国扇野螟属三条扇野螟Pleuroptya chlorophanta、枇杷扇野螟P.balteata、四斑扇野螟P.quadrimaculalis、淡黄扇野螟P.sabinusalis等4种老熟幼虫的的形态特征且提供幼虫的形态特征图,并给出基于幼虫的生物学习性。     

韩国斑蛾科三种幼虫的形态记述(鳞翅目)

在韩国己记述的斑蛾科昆虫有17种,这些斑蛾的幼虫主要食叶或卷叶,多数为害果树和林木等。幼虫身体短粗,头缩于前胸内,体上常有毛瘤或毛簇,腹足趾钩为单序中带。本文详细地记述了韩国斑蛾科Illiberis tenuis(Butler),Chalcosia remota(Walker)和Pryeria sinica Moore老熟幼虫的形态特征,并提供了形态特征图。     

韩国枝尺蛾亚科三种幼虫记述（鳞翅目：尺蛾科）

在韩国已记录527种尺蛾科成虫,本科的大多数幼虫是林业和农业害虫。本文详细记述了枝尺蛾亚科白厚尺蛾Pachyligia dolosa Butler、蜻蜓尺蛾Cystidia stratonice(Stoll)及角子尺蛾Planociampa antipala Prout幼虫的形态特征,并提供了形态特征图。标本存放在韩国江源大学校山林科学大学森林资源保护学科标本室。     

血清MCP-1、VE-cadherin和S-100蛋白水平变化对脑梗死患者病情的影响

探讨血清MCP-1、VE-cadherin和S-100蛋白水平变化对脑梗死患者病情的影响。选择2016年3月至2017年5月期间我院确诊的脑梗死患者60例作为研究对象,男性30例,女性30例,平均年龄为(60.2±8.1)岁。选择同期在我院进行体检的30名健康志愿者作为对照组。采用酶联免疫双抗体夹心法检测受试者血清MCP-1、VE-cadherin和S-100蛋白水平。脑梗死患者的血清MCP-1、VE-cadherin和S-100水平均高于对照组(p<0.05)。进展性脑梗死患者血清MCP-1、VE-cadherin和S-100水平显著升高(p<0.05)。轻型神经功能缺损患者的血清MCP-1、VE-cadherin和S-100水平显著低于中型和重型患者(p<0.05),中型患者血清MCP-1、VE-cadherin和S-100水平均显著低于重型患者(p<0.05)。脑梗死患者的血清MCP-1、VE-cadherin和S-100参与脑梗死的发生发展,并且与脑梗死患者的临床表现和神经功能缺损程度有关。     

韩国枝尺蛾亚科三种幼虫形态记述（鳞翅目：尺蛾科）

详细地记述了枝尺蛾亚科鞍形埃尺蛾Ectropiscrepuscularia (Denis&Schiffermller)、胡桃褶翅尺蛾Zamacrajuglansiaria (Graeser)及Phigaliaverecundaria (Leech)幼虫的形态特征 ,并提供了形态特征图。     

基于28S rRNA基因D2序列的优茧蜂亚科分子系统发育（膜翅目：茧蜂科）

The D2 variable region of 28S ribosomal RNA was sequenced from ethanol specimens or obtained from the literature to provide the first phylogenetie reconstruction of the subfamily Euphorinae (Hymenoptera;Braconidae). Phylogenetic relationships were established by comparing the results using two different methods (distance-based neighbor-joining, NJ; and maximum parsimony, MP) and three different outgroups. The monophyly of the Euphorinae is well supported by all trees generated from molecular data. All phylogenetic reconstructions yielded trees with very similar topologies that only partially resolved the morphologically defined tribes and the relationships within the subfamily. We found no evidence for the monophyletic natures of the tribes Euphorinl, Dinocampini,Perilitini, Syntretini, Comsophorini and Centisitini, but we did find some evidence for the tribes Meteorini and Microctonini. The monophyletic nature of the tribe Meteodnl was well-supported in all trees. We also found the clade containing the LecythodeUa,Microctonus, Orionis and Streblocera to be a monophyletic group, which corresponded to the tribe Microtonini, with Orionis transferred from the tribe Eupholini into Microtonini.Among the genera of Euphorini our results showed strong support for a paraphyletic nature of this group, which can be roughly divided into two clades, one consisting of Aridelus Wesmaelia, the other of Leiophron Peristenus, suggesting both of which may be given tribal rank. The placement of the genus Chrysopophorus is largely uncertain. Two clades,Dinocampus Perilitus and Cosmophorus Rhopalophorus, were constantly resolved in our analyses, with 42-96 and 97-100 bootstrap value support, respectively, suggesting that both of them form monophyletic groups. For members of the Centistini, Pygostolus may be removed and included in Microctonini or other relative tribe.