中国砖孢发属地衣研究

简短回顾了砖孢发展地衣研究概况,在已知的８种亚洲砖孢发属地衣中,中国有七种,其中两种为台湾特有,根据１５０余份标本的形态与化学特征,承认中国大陆砖孢发属地衣５种,指出了个别异名,发现亚洲砖孢发存在两个化学型,提供了中国５种砖孢发地衣分布的新资料,研究结果表明,５种砖孢发地衣集中分布于我国云南,四川和西藏东部,作者认为中国西南地区是砖孢发属地衣在亚洲的一个分布中心。     

云南小孢发属地衣的分类

报道了云南小孢发属地衣１３种,其中中国新记录种６个,它们是：喜马拉雅小孢发,尼泊尔小孢发,光滑小孢发,光亮小孢发,多叉小孢发,波氏小孢发,另外７种的形态及分布也在文中进行了讨论。     

中国砖格丝孢菌研究Ⅰ.砖格孢属

在进行中国砖格丝孢菌分类研究中,发现砖格孢属一个新种和两个中国新记录种。新种是泰山砖格孢Dictyosporium taishanensis,新记录种是索兰砖格孢Dictyosporium solani和七臂砖格孢Dictyosporium heptasporum。分别对三个种进行了中文描述,并附图,新种有拉丁文描述。研究标本保存在山东农业大学植物病理学标本室(HSAUP)。     

中国砖格丝孢菌研究I.砖格孢属

在进行中国砖格丝孢菌分类研究中,发现砖格孢属一个新种和两个中国新记录种。新种是泰山砖格孢Dictyosporiumtaishanensis,新记录种是索兰砖格孢Dictyosporiumsolani和七臂砖格孢Dictyosporiumheptasporum。分别对三个种进行了中文描述,并附图,新种有拉丁文描述。研究标本保存在山东农业大学植物病理学标本室(HSAUP)。     

中国砖格丝孢菌研究I.砖格孢属*

在进行中国砖格丝孢菌分类研究中,发现砖格孢属一个新种和两个中国新记录种。新种是泰山砖格孢Dictyosporiumtaishanensis,新记录种是索兰砖格孢Dictyosporiumsolani和七臂砖格孢Dictyosporiumheptasporum。分别对三个种进行了中文描述,并附图,新种有拉丁文描述。研究标本保存在山东农业大学植物病理学标本室(HSAUP)。     

中国砖格丝孢菌研究Ⅰ.砖格孢属

在进行中国砖格丝孢菌分类研究中,发现砖格孢属一个新种和两个中国新记录种.新种是泰山砖格孢Dictyosporium taishanensis, 新记录种是索兰砖格孢Dictyosporium solani和七臂砖格孢Dictyosporium heptasporum.分别对三个种进行了中文描述,并附图,新种有拉丁文描述.研究标本保存在山东农业大学植物病理学标本室(HSAUP).     

新疆小孢发属地衣的分类

根据多年的野外调查和相关的研究资料,对小孢发属(BryoriaB rodo & Hawksw.)地衣进行了分类学研究,报道新疆小孢发属地衣10种。其中Bryoria chalybeiformis,Bryoria fuscescens,Bryoria nadvornikiana和Bryo-riasimplicior为中国新记录种,Bryoria lanestris,Bryoria nitidula,Bryoria trichodes ssp.trichodes,Bryoria pseudo-fuscescens为新疆新记录种。     

新疆北部小孢发属地衣初步研究

新疆北部山脉生态环境多样,是地衣生长分布的理想区域。作者根据多年的实地调查和前人研究资料,对新疆北部小孢发属（Bryoria Brodo＆D．Hawksw．）地衣的种类及其分布、地理成分和垂直分布进行了初步研究。结果表明,分布在新疆北部的小孢发属地衣共有10种,它们分别是钢灰小孢发B．chalybeiformis（L．）Brodo＆D．Hawksw．、刺小孢发B．cortfusa（D．D．Awasthi）Brodo＆D．Hawksw．、叉小孢发B．furcellata（Fr．）Brodo＆D．Hawksw．、暗褐小孢发B．知伽cem（Gyeln．）Brodo＆D．Hawksw．、绵毛小孢发B．1anestris（Ach．）Brodo＆D．Hawksw．、B．nadvornikiana（Gyeln．）Brodo＆D．Hawksw．、光亮小孢发B．nhidula（1’h．Fr．）Brodo＆D．Hawksw．、假暗褐小孢发B．pseudofuscescens（Gyeln．）Brodo＆D．Hawksw．、单一小孢发B．simplicior（Vain．）Brodo＆D．Hawksw．及毛状小孢发原亚种B．trichodesssp．trichodes（Michx．）Brodo＆D．Hawksw．等,主要分布在天山和阿勒泰山。新疆北部小孢发属地衣可划分为3种地理成分：（1）环北极-高山种Bryonachalybeiformis、鼠nhidula、且furcellata;（2）环低北极及北方种B．simplicior;（3）环北方种B．加唧cew、B．1anestris、B．nadvornikiana、B．pseudofuscescens。阿勒泰山和天山的小孢发属地衣具明显的垂直分布差异。     

采自山东沂蒙山的砖格孢属—中国新记录种

在对山东淡水真菌的种类与分布的调查研究中,发现了砖格孢属的一个中国新记录种——锡兰砖格孢（Dictyosporium zeylanicum）。文中对该新记录种进行了描述、图示和讨论。     

太白山茶渍属地衣多孢种类的初步研究

对采自陕西太白山地区的中国茶渍属Lecanora地衣多孢种类进行了初步研究,报道三个中国新记录种:多孢茶渍L.bruneri,亚多孢茶渍L.cateilea和日本茶渍L.japonica。以往在中国发现的茶渍属地衣种类子囊均含有8孢,新报道的三种子囊中孢子常多于8个。本文中对每种都给出了详尽的形态、解剖和化学特征描述,并且提供了每种的外部形态照片和日本茶渍的多孢子囊显微照片。     

中国球粉衣属地衣

球粉衣属地衣主要分布在南半球,某些种分布于热带至亚热带。中国的球粉衣属地衣只在台湾有过记载,作者对云南、贵州、湖南的有关标本进行了形态与化学研究,并与来自澳大利亚的有关标本进行了比较,首次报道中国大陆球粉衣属的三个种:二型球粉衣,台湾球粉衣和黑果球粉衣。所有三个种均出现在云南,湖南只有黑果球粉衣,贵州只有台湾球粉衣。对这三个种的形态和化学中的某些问题进行了讨论,并根据文献资料提供了它们的世界分布。     

内蒙古亚洲小车蝗种群遗传多样性的微卫星分析

亚洲小车蝗Oedaleus asiaticus Bei-Bienko是我国北方草原和农牧交错区主要的害虫之一。为了从分子水平评价内蒙古地区亚洲小车蝗种群的遗传多样性和种群间遗传分化, 本文采用8对微卫星引物对内蒙古15个地点的亚洲小车蝗种群进行遗传多样性分析。结果表明： 各位点有效等位基因数为3.4517~13.2881, 多态信息含量值为0.5601~0.8563, Shannon氏多样性指数在0.7018~4.1789之间。15个种群的平均期望杂合度为0.6836, Nei氏期望杂合度为0.5303~0.6513, 群体遗传距离为0.1092~0.4235, 群体分化率Fst平均值为0.1612, 基因流Nm平均值为1.6164。8个微卫星位点均具有较高的多态性, 各种群间的遗传分化水平较大, 基因交流程度中等, 个体间的遗传变异大于种群间的遗传变异。15个不同地点的亚洲小车蝗种群根据遗传距离共聚为6支。种群间遗传分化与地理距离呈正相关关系。高山和沙漠对不同地区亚洲小车蝗种群的迁移具有阻碍作用, 可能是形成遗传分化的主要原因。研究结果从分子水平探索不同地区亚洲小车蝗种群间的内在联系, 为制定亚洲小车蝗的综合治理策略提供了分子生物学的基础资料。     

内蒙古天然草原不同放牧压力下亚洲小车蝗的食性及营养生态位（英文）

植物表皮蜡质中的饱和链烷作为内源指示剂广泛用于评价放牧家畜的食性和食量, 但用于天然草原蝗虫食性的评价研究较少。为了探讨天然草原蝗虫的食性及其生态位变化, 本研究以内蒙古天然草原为研究对象, 于2003年7-8月沿降水梯度选择3种典型植物群落（小针茅Stipa klemenzii、 羊草Leymus chinensis和大针茅Stipa grandis群落）, 在每个植物群落不同放牧压力下小区随机做20个植被样方, 样方内植物齐地面刈割, 测定其地上生物量和物种多样性, 取主要植物种测定其链烷模式, 同时采集放牧小区优势蝗虫种亚洲小车蝗Oedaleus asiaticus的粪便, 测定其链烷模式, 运用链烷技术评价蝗虫的食性及其营养生态位。结果表明： 不同植物群落中优势牧草种类及其比例不同, 其链烷模式存在种间差异, 链烷技术可以评价亚洲小车蝗的食性。亚洲小车蝗的食性在不同植物群落及不同放牧压力下存在显著的差异, 在羊草和大针茅群落中, 亚洲小车蝗是禾草采食者, 主要采食羊草和糙隐子草Cleistogenes squarrosa, 且与绵羊的营养生态位重叠指数较低, 分别为0.0619和0.0172; 在小针茅群落中亚洲小车蝗是杂类草采食者, 主要采食无芒隐子草Cleistogenes songorica、 猪毛菜Salsola collina和小针茅, 且与绵羊的营养生态位重叠指数较高, 达到0.1815。因此, 放牧不仅改变了群落的植物种类组成, 而且直接影响了亚洲小车蝗的食物组成, 二者对食物资源利用存在一定程度的竞争。     

Lecidea roseotincta, a new lichen species from Norway

Lecidea roseotincta sp. nov. is described from Norway. The species is mainly characterized by a white to grey or ± pink, amyloid thallus containing psoromic acid, black apothecia with a weakly developed proper margin, 16-spored asci without a well defined ocular chamber, and simple or 1-septate, medially constricted spores. It grows on bark of deciduous trees, mainly Alnus incana , in coastal sites.     

Chemical and serological properties of lipopolysaccharides from Vibrio parahaemolyticus O-untypeable strains isolated from patients

Chemical and serological studies have been carried out on the O-antigenic lipopolysaccharides (LPS) of six strains, U-6443, W-90144, X-3972, AD-7999, 90A-6611 and KX-V212, of Vibrio parahaemolyticus isolated from patients. The O-serotypes of these strains have not been identified because they were not agglutinated by any diagnostic antisera against known O-serotype strains. A compositional sugar analysis of their LPS revealed that out of the six O-untypeable (OUT) strains, U-6443, W-90144 and AD-7999 strains belonged to chemotype II (chemotype of O2), 90A-6611 and KX-V212 strains to chemotype III (chemotype of O3, O5, O11 and O13) and X-3972 strain to chemotype IV (chemotype of O4). A structural analysis of LPS isolated from KX-V212 revealed that the inner core region of the LPS consisted of only one mole of 2-keto-3-deoxy-D-manno-octonic acid, which carried a phosphate group at position C4 and the outer core at position C5. In passive hemolysis tests performed by using LPS as the antigen to sensitize sheep red blood cells (SRBC), and diagnostic antisera (O1 to O11) or anti-whole-cell rabbit antisera raised against O12, O13 and the six OUT strains, strong cross-reactivity was observed among LPS derived from the strains belonging to chemotype II (U-6443, W-90144, AD-7999 and O2). Strong cross-reactivity was also observed between X-3972 (chemotype IV) and O4 LPS. In contrast, LPS from two of the strains belonging to chemotype III (90A-6611 and KX-V212) did not react with any of the antisera raised against known O-serotypes. Cross-absorption tests showed that the O-antigens of U-6443, W-90144 and AD-7999 were identical to that of O2, and the O-antigen of X-3972 to that of O4. On the other hand, after the absorption of antisera raised against 90A-6611 and KX-V212 with O2 cells, the hemolytic activities against SRBC sensitized with homologous LPS were still retained at a high titer, whereas the hemolytic activities against SRBC sensitized with LPS from other O-serotype strains were completely eliminated. A cross-absorption test revealed that the O-antigens of these two strains were identical to each other. Thus, it was demonstrated that the O-serotype of OUT strains 90A-6611 and KX-V212 was not involved in the known O-serotypes; rather it represented a novel serotype which has not hitherto been reported.     

中国新近纪几种稀有啮齿动物

30多年来,中国新近纪堆积物中发现了大量的小哺乳动物化石,但一些种类的材料很少,而且仅在个别地点出现,在动物群中常以未定属、种记述.详细描述了4种罕见的啮齿动物,其中3种,泗洪豪鼠(Sayimys sihongensis)、亚洲别齿始鼠(Apeomys asiaticus)和新来鼠(未定种)(Neocometes sp.)发现于江苏泗洪早中新世下草湾组,一种,细小云南始鼠(Yuneomys pusillus)产自云南禄丰晚中新世石灰坝组.与欧亚或北美有关材料的比较研究表明,江苏泗洪标本无法归入相关属的任何已知种,因此被确定了两个新种(S.sihongensis和A.asiaticus);禄丰原归入Leptodontomys pusillus的标本与该属的特征不符,被指定为新属Ynueomys.泗洪的A.asiaticus和Neocometes sp.分别代表该属在亚洲和中国的首次记录;它们与S.sihongensis在亚洲远东地区的出现,增加了我们对这些稀有动物地理分布和欧亚古生物地理关系的知识.禄丰的新属Yuneomys与共生的Plesieomys和Heteroeomys属同为丘齿型始鼠类,可能都属热带或亚热带森林型动物;在禄丰动物群中缺少脊齿型始鼠类,与华北动物群的组成不同,似乎表明华南和华北始鼠动物群在中新世期间已有明显的分异,进而支持这一时期亚洲出现了不同生态区系的设想.     

光周期对内蒙古三种草原蝗虫高龄若虫发育、存活、羽化、生殖的影响

研究不同光周期(L8∶D16、L12∶D12及L16∶D8)对毛足棒角蝗Dasyhippus barbipes(F.-W.),亚洲小车蝗Oedaleus decorus asiaticus B.-Bidenko,小翅雏蝗Chorthippus fallax(Zub.)3种草原蝗虫高龄若虫的发育、存活、羽化、生殖的影响。结果表明:在白天温度(28±1)℃,黑夜温度(23±1)℃的恒定温度下,不同光周期对毛足棒角蝗和亚洲小车蝗高龄若虫的发育、羽化、产卵影响不大,但是对其存活率有极显著的影响:毛足棒角蝗和亚洲小车蝗高龄若虫到成虫的发育速度在中光照下(L12∶D12)最快。而在短光照(L8∶D16)下更有利于小翅雏蝗若虫发育,其次是中光照;毛足棒角蝗的羽化在中光照条件下最适宜,而长光照时数(L16∶D8)更有利于亚洲小车蝗和小翅雏蝗的羽化;光周期对亚洲小车蝗产卵影响最为明显,毛足棒角蝗和亚洲小车蝗在中光照和长光照时数条件下有利于它们产卵,而小翅雏蝗在短光照和中光照时数下有利于产卵。     

Chemical variation in the lichen genus <Emphasis Type="Italic">Letrouitia</Emphasis> (Ascomycota,Letrouitiaceae)

Secondary metabolites from 193 specimens belonging to 15 species of Letrouitia were analyzed by HPLC. Significant quantities of the anthraquinones parietin and fragilin were found in most species and occasionally minor quantities of emodin, 5-chloroemodin, 7-chloroemodin, 7-chloroteloschistin, 7-chlorofallacinal and 7-chloroparietinic acid were present. Eight previously unknown lichen substances were identified. A chemotype containing seven new dibenzofurans (8-chlorodioxocondidymic acid, 8-chlorodioxodidymic acid, 8-chloroxodidymic acid, dioxocondidymic acid, dioxodidymic acid, letrouitic acid and oxodidymic acid) was found in 3 species. In addition, a chemotype containing four unknowns (possibly chlorodepsidones) occurred in L. subvulpina. Eight different chemotypes were identified in the genus. The secondary chemistry was important for the precise identification of some species in Letrouitia. The similarity in secondary chemistry between Letrouitiaceae and Teloschistaceae is not particularly strong, as the shared compounds are also known to occur in several other lichen families.     

Chemical and molecular characterization of Phomopsis and Cytospora-like endophytes from different host plants in Brazil

Phomopsis and related taxa comprise important endophytic and plant pathogenic species, and are known for the production of a diverse array of secondary metabolites. Species concepts within this group based on morphological characters and assumed host specificity do not reflect phylogenetic affinities. Additional phenotypic characters, such as profiles of secondary metabolites, are needed for practical species recognition. We investigated 36 strains of Phomopsis spp. and Cytospora-like fungi, obtained as endophytes of different host plants in Brazil, using metabolite profiling based on HPLC-UV/liquid chromatography -mass spectrometry (LC-MS) combined with cluster analysis of the results. Strains were also subjected to phylogenetic analyses based on internal transcribed spacer (ITS) rDNA. Six chemotypes were identified. Chemotypes 1-5 contained Phomopsis strains, while Cytospora-like strains formed the chemotype 6. Strains of chemotype 1 typically produced alternariols, altenusin, altenuene, cytosporones, and dothiorelones. Alternariol and seven unknown compounds were consistently produced by strains of chemotype 2. Members of chemotypes 3-5 produced poor metabolite profiles containing few chemical markers. Cytospora-like endophytes (chemotype 6) produced a characteristic set of metabolites including cytosporones and dothiorelones. Bayesian and Maximum Parsimony (MP) trees classified strains of each chemotype into single phylogenetic lineages or closely related groups. Strains of chemotypes 1 and 2 formed a monophyletic group along with Diaporthe neotheicola. The remaining Phomopsis strains formed monophyletic (chemotype 4) or polyphyletic (chemotypes 3 and 5) lineages inside a large and well supported clade. Cytospora-like strains formed a monophyletic lineage located at an intermediary position between Diaporthe/Phomopsis and Valsa/Cytospora clades. The combined results show that the production of secondary metabolites by Phomopsis and related Diaporthales may be species-specific, giving support to the use of metabolite profiling and chemical classification for phenotypic recognition and delimitation of species.