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1.
Joong Hyoun Chin Xiaochun Lu Stephan M. Haefele Rico Gamuyao Abdelbagi Ismail Matthias Wissuwa Sigrid Heuer 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(6):1087-1088
Marker-assisted breeding is a very useful tool for breeders but still lags behind its potential because information on the
effect of quantitative trait loci (QTLs) in different genetic backgrounds and ideal molecular markers are unavailable. Here,
we report on some first steps toward the validation and application of the major rice QTL Phosphate uptake 1 (Pup1) that confers tolerance of phosphorus (P) deficiency in rice (Oryza sativa L.). Based on the Pup1 genomic sequence of the tolerant donor variety Kasalath that recently became available, markers were designed that target
(1) putative genes that are partially conserved in the Nipponbare reference genome and (2) Kasalath-specific genes that are
located in a large insertion-deletion (INDEL) region that is absent in Nipponbare. Testing these markers in 159 diverse rice
accessions confirmed their diagnostic value across genotypes and showed that Pup1 is present in more than 50% of rice accessions adapted to stress-prone environments, whereas it was detected in only about
10% of the analyzed irrigated/lowland varieties. Furthermore, the Pup1 locus was detected in more than 80% of the analyzed drought-tolerant rice breeding lines, suggesting that breeders are unknowingly
selecting for Pup1. A hydroponics experiment revealed genotypic differences in the response to P deficiency between upland and irrigated varieties
but confirmed that root elongation is independent of Pup1. Contrasting Pup1 near-isogenic lines (NILs) were subsequently grown in two different P-deficient soils and environments. Under the applied
aerobic growth conditions, NILs with the Pup1 locus maintained significantly higher grain weight plant−1 under P deprivation in comparison with intolerant sister lines without Pup1. Overall, the data provide evidence that Pup1 has the potential to improve yield in P-deficient and/or drought-prone environments and in diverse genetic backgrounds. 相似文献
2.
Marker-assisted selection to introgress rice QTLs controlling root traits into an Indian upland rice variety 总被引:9,自引:0,他引:9
Steele KA Price AH Shashidhar HE Witcombe JR 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(2):208-221
A marker-assisted back-crossing (MABC) breeding programme was conducted to improve the root morphological traits, and thereby
drought tolerance, of the Indian upland rice variety, Kalinga III. This variety, the recurrent parent in the MABC, had not
previously been used for quantitative trait locus (QTL) mapping. The donor parent was Azucena, an upland japonica variety from Philippines. Five segments on different chromosomes were targeted for introgression; four segments carried QTLs
for improved root morphological traits (root length and thickness) and the fifth carried a recessive QTL for aroma. Some selection
was made at non-target regions for recurrent parent alleles. We describe the selection made in three backcross (BC) generations
and two further crosses between BC3 lines to pyramid (stack) all five target segments. Pyramids with four root QTLs were obtained in eight generations, completed
in 6 years using 3,000 marker assays in a total of 323 lines. Twenty-two near-isogenic lines (NILs) were evaluated for root
traits in five field experiments in Bangalore, India. The target segment on chromosome 9 (RM242-RM201) significantly increased
root length under both irrigated and drought stress treatments, confirming that this root length QTL from Azucena functions
in a novel genetic background. No significant effects on root length were found at the other four targets. Azucena alleles
at the locus RM248 (below the target root QTL on chromosome 7) delayed flowering. Selection for the recurrent parent allele
at this locus produced early-flowering NILs that were suited for upland environments in eastern India. 相似文献
3.
Matthias Wissuwa 《Plant and Soil》2005,269(1-2):57-68
The Pup1 locus confers tolerance to phosphorus (P) deficiency in rice (Oryza sativa L.). Transferring the Pup1 locus to an intolerant genotype increased P uptake by a factor 3 to 4. Lines with the Pup1 locus maintained higher root growth rates under P deficiency, but only as they started to diverge from intolerant lines in
P uptake. It was thus not possible to determine if differences in root growth preceded and caused differences in P uptake
or whether high root growth was the result of higher external P uptake efficiency (P influx per root size). The purpose of
this paper is to review experimental evidence on the effect of Pup1 in light of recent results in modelling cause-and-effect relations between root growth, external efficiency and P uptake.
Model simulations suggested that only very small changes in factors enhancing root growth were needed to explain the effect
of Pup1 on P uptake. A 22% increase in root fineness or in internal P utilization efficiency (root dry matter per root P) was sufficient
to triple P uptake . External root efficiency had to increase by 33 to account for the effect of Pup1. However, the most noticeable effect of increases in external efficiency was a subsequent stimulation of root growth that
contributed eight times more to final P uptake compared to the change in external efficiency. Comparisons of model simulations
with empirical data suggested that measured differences in external efficiency between Nipponbare and NIL-Pup1 were sufficiently large to account for the increase in P uptake. A segregation analysis using several pairs of contrasting
NILs (at the Pup1 locus) further confirmed this as Pup1 co-segregated with external efficiency but not with seedling root growth or internal efficiency. 相似文献
4.
Identification and functional analysis of a locus for improvement of lodging resistance in rice 总被引:38,自引:0,他引:38 下载免费PDF全文
We identified a new locus responsible for increased pushing resistance of the lower part of rice (Oryza sativa) and analyzed its physiological function to understand how to improve lodging resistance in rice. Quantitative trait loci (QTLs) controlling pushing resistance of the lower part were analyzed in a population of backcross inbred lines of japonica Nipponbare x indica Kasalath plants cut out at 40 cm to exclude the effect of the weight of the upper parts. Five QTLs for pushing resistance were detected; only one QTL from Kasalath on chromosome 5 (prl5) had a positive effect. The likelihood odds ratio curve of prl5 echoed that for lodging resistance by typhoon. We selected three near-isogenic lines (NILs) in which the chromosomal region of prl5 was substituted with that of Kasalath in the Nipponbare background. The dry weights and densities and the contents of accumulated carbohydrate in stems below 40 cm (lower stems) in each NIL were significantly higher than those of Nipponbare. There was no difference between Nipponbare and the NILs in yield, root characteristics, or the weights of the upper parts. Pushing resistance of the lower part and lodging resistance in the NILs were up to twice as high as in Nipponbare. These results suggest that prl5 might affect the characteristics of the lower stems of the NILs, thus increasing lodging resistance. 相似文献
5.
Improvement of lodging resistance with QTLs for stem diameter in rice (Oryza sativa L.) 总被引:3,自引:0,他引:3
Kashiwagi T Togawa E Hirotsu N Ishimaru K 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,117(5):749-757
Varietal differences among ten rice cultivars showed that stem diameter is a key factor in lodging resistance (measured in terms of pushing resistance). Two near-isogenic lines (NILs) were selected from a series of chromosome segment substitution lines developed between cultivars Nipponbar and Kasalath, one containing a single stem diameter QTL (sdm8; NIL114), and another with four stem diameter QTLs (sdm1, sdm7, sdm8, sdm12; NIL28). Compared with the Nipponbare control, stem diameters were larger in NIL114 and NIL28 by about 7 and 39%, respectively. Pushing resistance in NIL28 was significantly greater than in Nipponbare, but NIL114 was similar to Nipponbare. The two NILs had greater weight of lower stem and culm wall thickness than Nipponbare. NIL28 had higher plant height, which is a negative effect on lodging resistance, than Nipponbare. The non-structural carbohydrate contents of NIL stems were higher than that of Nipponbare, whereas the silicon contents were lower in the NILs, and cellulose contents were lower only in NIL28. The basal internodes of the two NILs were significantly stiffer than those of Nipponbare. These results suggest that increasing stem diameter in rice breeding programs would improve lodging resistance, although the combination of multiple QTLs would be necessary to produce thicker stems with higher pushing resistance, whereas the higher plant height could also result from the combination of multiple QTLs. 相似文献
6.
Joong Hyoun Chin Xiaochun Lu Stephan M. Haefele Rico Gamuyao Abdelbagi Ismail Matthias Wissuwa Sigrid Heuer 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(6):1073-1086
Marker-assisted breeding is a very useful tool for breeders but still lags behind its potential because information on the effect of quantitative trait loci (QTLs) in different genetic backgrounds and ideal molecular markers are unavailable. Here, we report on some first steps toward the validation and application of the major rice QTL Phosphate uptake 1 (Pup1) that confers tolerance of phosphorus (P) deficiency in rice (Oryza sativa L.). Based on the Pup1 genomic sequence of the tolerant donor variety Kasalath that recently became available, markers were designed that target (1) putative genes that are partially conserved in the Nipponbare reference genome and (2) Kasalath-specific genes that are located in a large insertion-deletion (INDEL) region that is absent in Nipponbare. Testing these markers in 159 diverse rice accessions confirmed their diagnostic value across genotypes and showed that Pup1 is present in more than 50% of rice accessions adapted to stress-prone environments, whereas it was detected in only about 10% of the analyzed irrigated/lowland varieties. Furthermore, the Pup1 locus was detected in more than 80% of the analyzed drought-tolerant rice breeding lines, suggesting that breeders are unknowingly selecting for Pup1. A hydroponics experiment revealed genotypic differences in the response to P deficiency between upland and irrigated varieties but confirmed that root elongation is independent of Pup1. Contrasting Pup1 near-isogenic lines (NILs) were subsequently grown in two different P-deficient soils and environments. Under the applied aerobic growth conditions, NILs with the Pup1 locus maintained significantly higher grain weight plant?1 under P deprivation in comparison with intolerant sister lines without Pup1. Overall, the data provide evidence that Pup1 has the potential to improve yield in P-deficient and/or drought-prone environments and in diverse genetic backgrounds. 相似文献
7.
M. Wissuwa M. Yano N. Ae 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):777-783
Phosphorus (P) deficiency of soils is a major yield-limiting factor in rice production. Increasing the P-deficiency tolerance
of rice cultivars may represent a more cost-effective solution than relying on fertilizer application. The objective of this
study was to identify putative QTLs for P-deficiency tolerance in rice, using 98 backcross inbred lines derived from a japonica×indica cross and genotyped at 245 RFLP marker loci. Lines were grown on P-deficient soil and P uptake, internal P-use efficiency,
dry weight, and tiller number were determined. Three QTLs were identified for dry weight and four QTLs for P uptake, together
explaining 45.4% and 54.5% of the variation for the respective traits. Peaks for both traits were in good agreement which
was to be expected considering the tight correlation of r=0.96 between dry weight and P uptake. For both traits the QTL linked to marker C443 on chromosome 12 had a major effect.
Two of the three QTLs detected for internal P-use efficiency, including the major one on chromosome 12, coincided with QTLs
for P uptake; however, whereas indica alleles increased P uptake they reduced P-use efficiency. We concluded that this was not due to the tight linkage of two
genes in repulsion but rather due to an indirect effect of P uptake on P-use efficiency. Most lines with high use efficiency
were characterized by very low P uptake and dry weight and apparently experienced extreme P-deficiency stress. Their higher
P-use efficiency was thus the result of highly sub-optimal tissue-P concentrations and did not represent a positive adaptation
to low P availability. The number of tillers produced under P deficiency is viewed as an indirect indicator of P-deficiency
tolerance in rice. In addition to the major QTL on chromosome 12 already identified for all other traits, two QTLs on chromosome
4 and 12 were identified for tiller number. Their position, however, coincided with QTLs for tiller number reported elsewhere
under P-sufficient conditions and therefore appear to be not related to P-deficiency tolerance. In this study P-deficiency
tolerance was mainly caused by differences in P uptake and not in P-use efficiency. Using a trait indirectly related to P-deficiency
tolerance such as tiller number, we detected a major QTL but none of the minor QTLs detected for P uptake or dry weight.
Received: 9 February 1998 / Accepted: 29 April 1998 相似文献
8.
Mapping QTLs and candidate genes for rice root traits under different water-supply conditions and comparative analysis across three populations 总被引:12,自引:0,他引:12
Zheng BS Yang L Zhang WP Mao CZ Wu YR Yi KK Liu FY Wu P 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,107(8):1505-1515
To investigate the genetic factors underlying constitutive and adaptive morphological traits of roots under different water-supply conditions, a recombinant inbred line (RIL) population derived from a cross between the lowland rice variety IR1552 and the upland rice variety Azucena with 249 molecular markers, was used in cylindrical-pot experiments. Eighteen QTLs were detected for seminal root length (SRL), adventitious root number (ARN), and lateral root length (LRL) and lateral root number (LRN) on the seminal root at a soil depth of from 3 to 6 cm under flooding and upland conditions. One identical QTL was detected under both flooding and upland conditions. The relative parameters under the two water-supply conditions were also used for QTL analysis. Five QTLs for upland induced variations in the traits were detected with the positive alleles from Azucena. A comparative analysis was performed for the QTLs detected in this study and those reported from two other populations with Azucena as a parent. Several identical QTLs for root elongation were found across the three populations with positive alleles from Azucena. Candidate genes were screened from ESTs and cDNA-AFLP clones for comparative mapping with the detected QTLs. Two genes for cell expansion, OsEXP2 and endo-1,4--D-glucanase EGase, and four cDNA-AFLP clones from root tissues of Azucena, were mapped on the intervals carrying the QTLs for SRL and LRL under upland conditions, respectively.Communicated by H.C. Becker 相似文献
9.
应用AFLP与RFLP标记研究水稻磷吸收与利用率的数量性状位点 总被引:4,自引:0,他引:4
To identify genetic factors underlying phosphorus (P) uptake and use efficiency under low-P stress in rice (Oryza sativa L.), 84 selected genotypes (recombinant inbred lines) and their parents (which differed in tolerance for low-P stress) “IR20” and IR55178-3B-9-3, were cultured in liquid medium supplemented with adequate and low P levels in a greenhouse. Plants were sampled after 6 weeks in culture for measurements of plant dry weight, P concentration, P uptake and P use efficiency under both P sufficient and stress conditions. A total of 179 molecular markers, including 26 RFLPs and 153 AFLPs, mapped on all 12 chromosomes of rice based on the 84 selected genotypes were used to detect the quantitative trait loci (QTLs) underlying tolerance for low-P stress. Three QTLs were detected on chromosomes 6, 7 and 12, respectively, for relative plant dry weight (RPDW) and relative P uptake (RPUP). One of the QTLs flanked by RG9 and RG241 on chromosome 12 had a major effect which explained about 50% of the variations in the two parameters across the population. The results coincided with the QTLs for low-P stress based on relative tillering ability from the same population from a cross between Nipponbare and Kasalath under soil condition. The identical major QTL for P uptake and plant growth under low-P stress in both liquid medium and soil strongly suggests that the ability of P uptake mainly controls rice tolerance for low-P stress. 相似文献
10.
QTL mapping of root hair and acid exudation traits and their relationship to phosphorus uptake in common bean 总被引:4,自引:0,他引:4
Xiaolong Yan Hong Liao Steve E. Beebe Matthew W. Blair Jonathan P. Lynch 《Plant and Soil》2004,265(1-2):17-29
The relationship between root-hair growth, acid exudation and phosphorus (P) uptake as well as the quantitative trait loci (QTLs) associated with these traits were determined for a recombinant inbred line (RIL) population derived from the cross of two contrasting common bean (Phaseolus vulgaris L.) genotypes, DOR364 and G19833, which were grown in solution culture and under field conditions with low-P availability. In the solution-culture study, root-hair density, root-hair length, H+ exudation and total acid exudation were measured. Substantial genotypic variability was observed for these traits and their response to P availability. The P-efficient parent G19833 had greater root-hair density, longer root-hair length, and greater exudation of H+ and total acid than the P-inefficient genotype DOR364. These traits segregated continuously in the RIL population, with obvious tendency of trait transgression. Genetic analysis revealed that the root traits measured had various heritabilities, with h
b
2
ranging from 43.24 to 86.70%. Using an integrated genetic map developed for the population, a total of 19 QTLs associated with root hair, acid exudation and P-uptake traits were detected on 8 linkage groups. P uptake in the field was positively correlated with total acid exudation, basal root-hair length, and basal root-hair density. Acid-exudation traits were intercorrelated, as were root-hair traits. Total acid exudation was positively correlated with basal root-hair density and length. Linkage analysis revealed that some of the root-trait QTLs were closely linked with QTLs for P uptake in the field. We propose that marker-assisted selection (MAS) might be a feasible alternative to conventional screening of phenotypic root traits. 相似文献
11.
Comparison of a set of allelic QTL-NILs for chromosome 4 of tomato: Deductions about natural variation and implications for germplasm utilization 总被引:13,自引:5,他引:8
A. J. Monforte E. Friedman D. Zamir S. D. Tanksley 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(4):572-590
Quantitative Trait Locus (QTL) allelic variation was studied by analyzing near-isogenic lines (NILs) carrying homologous introgressions
on chromosome 4 from three green-fruited wild tomato species. The NILs affect agronomic (yield, brix, fruit weight) and fruit (fruit shape,
color, epidermal reticulation) traits in a similar manner. However, significant differences were detected in the magnitudes
of the effects, the dominance deviations and epistatic interactions, indicating that those species carry different alleles
for the QTL. As the QTL did not show any interaction across environments, gene-tic backgrounds or other QTLs, it can be used
to introduce novel genetic variation into a broad range of cultivars. Analysis of new recombinant NILs showed that fruit traits
are controlled by several linked genetic loci, whereas multiple genetic loci control the agronomic traits within the original
introgression. The hypothesis that QTLs may be composed of multiple linked genes can not be rejected prior to implement projects
for QTL isolation and cloning. Loci involved in color enhancement could not be related to any known gene involved in the carotenoid
biosynthesis pathway, therefore it is hypothesized that the function of those loci must be related to the genetic regulation
of the carotenoid biosynthetic pathway.
Received: 14 April 2000 / Accepted: 12 May 2000 相似文献
12.
H. X. Lin T. Yamamoto T. Sasaki M. Yano 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(7):1021-1028
To characterize quantitative trait loci (QTLs), we used marker-assisted selection (MAS) to develop three nearly isogenic lines
(NILs) differing only for the presence of a single, specific QTL (QTL-NILs) –Hd1, Hd2, and Hd3 – for heading date in rice. The three lines contained the chromosomal region of the target QTL from donor variety Kasalath(indica) in the genetic background of var. Nipponbare (japonica). To analyze epistatic interactions in pairs of these QTLs, we also used MAS to develop four combined QTL-NILs with 2 of
the 3 QTLs or with all 3. Different daylength treatment testing of the QTL-NILs revealed that the three QTLs control photoperiod
sensitivity. Genetic analysis of F2 populations derived from crosses between the three QTL-NILs with a single QTL using molecular markers revealed the existence
of epistatic interactions between Hd1 and Hd2, and Hd2 and Hd3. These interactions were also confirmed by the analysis of combined QTL-NILs under different daylength conditions. The existence
of an epistatic interaction between Hd1 and Hd3 was also clarified. Based on these results, we suggest that the Kasalath allele of Hd3 does not affect photoperiod sensitivity by itself but that it is involved in enhancement of the expression of the Nipponbare
alleles of Hd1 and Hd2.
Received: 22 October 1999 / Accepted: 21 March 2000 相似文献
13.
Mapping quantitative trait loci controlling seed dormancy and heading date in rice, Oryza sativa L., using backcross inbred lines 总被引:21,自引:0,他引:21
S. Y. Lin T. Sasaki M. Yano 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(8):997-1003
To detect quantitative trait loci (QTLs) controlling seed dormancy, 98 BC1F5 lines (backcross inbred lines) derived from a backcross of Nipponbare (japonica)/Kasalath (indica)//Nipponbare were analyzed genetically. We used 245 RFLP markers to construct a framework linkage map. Five putative QTLs
affecting seed dormancy were detected on chromosomes 3, 5, 7 (two regions) and 8, respectively. Phenotypic variations explained
by each QTL ranged from 6.7% to 22.5% and the five putative QTLs explained about 48% of the total phenotypic variation in
the BC1F5 lines. Except for those of the QTLs on chromosome 8, the Nipponbare alleles increased the germination rate. Five putative
QTLs controlling heading date were detected on chromosomes 2, 3, 4, 6 and 7, respectively. The phenotypic variation explained
by each QTL for heading date ranged from 5.7% to 23.4% and the five putative QTLs explained about 52% of the total phenotypic
variation. The Nipponbare alleles increased the number of days to heading, except for those of two QTLs on chromosomes 2 and
3. The map location of a putative QTL for heading date coincided with that of a major QTL for seed dormancy on chromosome
3, although two major heading-date QTLs did not coincide with any seed dormancy QTLs detected in this study.
Received: 10 October 1997 / Accepted: 12 January 1998 相似文献
14.
Evaluation of near-isogenic lines of rice introgressed with QTLs for root depth through marker-aided selection 总被引:11,自引:0,他引:11
L. Shen B. Courtois K. L. McNally S. Robin Z. Li 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(1):75-83
Drought is one of the main abiotic constraints in rice. A deep root system contributes efficiently to maintaining the water
status of the crop through a stress period. After identifying QTLs affecting root parameters in a doubled-haploid (DH) population
of rice derived from the cross IR64/Azucena, we started a marker-assisted backcross program to transfer the Azucena allele
at four QTLs for deeper roots (on chromosomes 1, 2, 7 and 9) from selected DH lines into IR64. We selected the backcross progenies
strictly on the basis of their genotypes at the marker loci in the target regions up to the BC3F2. We assessed the proportion of alleles remaining from Azucena in the non-target areas of the BC3F2 plants, which was in the range expected for the backcross stage reached. Twenty nine selected BC3F3 near-isogenic lines (NILs) were developed and compared to IR64 for the target root traits and three non-target traits in
replicated experiments. Of the three tested NILs carrying target 1, one had significantly improved root traits over IR64.
Three of the seven NILs carrying target 7 alone, as well as three of the eigth NILs carrying both targets 1 and 7, showed
significantly improved root mass at depth. Four of the six NILs carrying target 9 had significantly improved maximum root
length. Five NILs carrying target 2 were phenotyped, but none had a root phenotype significantly different from that of IR64.
A re-analysis of the initial data with the composite interval mapping technique revealed two linked QTLs with opposite effects
in this area. Some NILs were taller than IR64 and all had a decreased tiller number because of a likely co-introgression of
linked QTLs. The usefulness of NILs, the efficiency of marker-aided selection for QTLs and the relationship between root traits
are discussed. The NILs with an improved root system will permit testing the importance of root depth for water-limited environments.
Received: 17 July 2000 / Accepted: 20 October 2000 相似文献
15.
QTL mapping of root traits in a doubled haploid population from a cross between upland and lowland japonica rice in three environments 总被引:7,自引:0,他引:7
Li Z Mu P Li C Zhang H Li Z Gao Y Wang X 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,110(7):1244-1252
To genetically dissect drought resistance associated with japonica upland rice, we evaluated a doubled haploid (DH) population from a cross between two japonica cultivars for seven root traits under three different growing conditions (upland, lowland and upland in PVC pipe). The traits included basal root thickness (BRT), total root number (RN), maximum root length (MRL), root fresh weight (RFW), root dry weight (RDW), ratio of root fresh weight to shoot fresh weight (RFW/SFW) and ratio of root dry weight to shoot dry weight (RDW/SDW). The BRT was significantly correlated with the index of drought resistance, which was defined as the ratio of yield under the stress of the upland condition to that under the normal lowland condition. A complete genetic linkage map with 165 molecular markers covering 1,535 cM was constructed. Seven additive quantitative trait loci (QTLs) and 15 pairs of epistatic loci for BRT and RN were identified under upland and lowland conditions, and 12 additive QTLs and 17 pairs of epistatic QTLs for BRT, RN, MRL, RFW, RFW/SFW and RDW/SDW were identified under the PVC pipe condition. Four additive QTLs and one pair of epistatic QTLs controlling IDR were also found. These QTLs individually explained up to 25.6% of the phenotypic variance. QTL × environment (Q × E) interactions were detected for all root traits, and the contributions of these interactions ranged from 1.1% to 19.9%. Five co-localized QTLs controlling RFW and RDW, RFW/SFW, RDW/SDW and IDR, BRT and RN, RN, MRL and IDR were found. Four types of QTLs governing BRT and RN were classified by their detection in the upland and lowland conditions. Some common QTLs for root traits across different backgrounds were also revealed. These co-localized QTLs and common QTLs will facilitate marker-assisted selection for root traits in rice breeding programs. 相似文献
16.
QTL analysis and mapping of pi21, a recessive gene for field resistance to rice blast in Japanese upland rice 总被引:14,自引:0,他引:14
S. Fukuoka K. Okuno 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(2-3):185-190
Field resistance is defined as the resistance that allows effective control of a parasite under natural field conditions and
is durable when exposed to new races of that parasite. To identify the genes for field resistance to rice blast, quantitative
trait loci (QTLs) conferring field resistance to rice blast in Japanese upland rice were detected and mapped using RFLP and
SSR markers. QTL analysis was carried out in F4 progeny lines from the cross between Nipponbare (moderately susceptible, lowland) and Owarihatamochi (resistant, upland).
Two QTLs were detected on chromosome 4 and one QTL was detected on each of chromosomes 9 and 12. The phenotypic variation
explained by each QTL ranged from 7.9 to 45.7% and the four QTLs explained 66.3% of the total phenotypic variation. Backcrossed
progeny lines were developed to transfer the QTL with largest effect using the susceptible cultivar Aichiasahi as a recurrent
parent. Among 82 F3 lines derived from the backcross, resistance segregated in the expected ratio of resistant 1 : heterozygous 2 : susceptible
1. The average score for blast resistance measured in the field was 4.2 ± 0.67, 7.5 ± 0.51and 8.2 ± 0.66, for resistant, heterozygous
and susceptible groups, respectively. The resistance gene, designated pi21, was mapped on chromosome 4 as a single recessive gene between RFLP marker loci G271 and G317 at a distance of 5.0 cM and 8.5 cM, respectively. The relationship to previously reported major genes and QTLs conferring
resistance to blasts, and the significance of marker-assisted selection to improve field resistance, are discussed.
Received: 8 June 2000 / Accepted: 24 November 2000 相似文献
17.
K. A. Steele A. H. Price J. R. Witcombe Roshi Shrestha B. N. Singh J. M. Gibbons D. S. Virk 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(1):101-108
Altering root morphology of rice (Oryza sativa L.) cultivars could improve yields in drought-prone upland ecosystems. Marker-assisted backcross breeding was used to introgress four QTLs for root traits into an upland rice cultivar. The QTLs had previously been identified under experimental conditions in a different genetic background. The introgressed lines and the recurrent parent were grown for 6 years by resource-poor farmers in upland sites in Eastern India and yields recorded. In combination the QTLs significantly increased yield by 1 t ha?1 under relatively favourable field conditions. In less favourable trials, the QTL effects were not detected due to greater heterogeneity in soil–water availability in very low yielding environments and consequent yield variability. Root studies under controlled conditions showed that lines with the introgressions had longer roots throughout tillering than the recurrent parent (14 cm longer 2 weeks after sowing). Therefore, both improved roots and increased yield can be attributed to the introgression of QTLs. This is the first demonstration that marker-assisted backcross breeding (MABC) to introgress multiple root QTLs identified under controlled conditions is an effective strategy to improve farmers’ yields of upland rice. The strategy was used to breed a novel upland rice cultivar that has been released in India as Birsa Vikas Dhan 111. 相似文献
18.
Kiyosumi Hori Kazuhiko Sugimoto Yasunori Nonoue Nozomi Ono Kazuki Matsubara Utako Yamanouchi Akira Abe Yoshinobu Takeuchi Masahiro Yano 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(8):1547-1557
Backcrossed inbred lines (BILs) and a set of reciprocal chromosome segment substitution lines (CSSLs) derived from crosses
between japonica rice cultivars Nipponbare and Koshihikari were used to detect quantitative trait loci (QTLs) for pre-harvest sprouting resistance.
In the BILs, we detected one QTL on chromosome 3 and one QTL on chromosome 12. The QTL on the short arm of chromosome 3 accounted
for 45.0% of the phenotypic variance and the Nipponbare allele of the QTL increased germination percentage by 21.3%. In the
CSSLs, we detected seven QTLs, which were located on chromosomes 2, 3 (two), 5, 8 and 11 (two). All Nipponbare alleles of
the QTLs were associated with an increased rate of germination. The major QTL for pre-harvest sprouting resistance on the
short arm of chromosome 3 was localized to a 474-kbp region in the Nipponbare genome by the SSR markers RM14240 and RM14275
by using 11 substitution lines to replace the different short chromosome segments on chromosome 3. This QTL co-localized with
the low-temperature germinability gene qLTG3-1. The level of germinability under low temperature strongly correlated with the level of pre-harvest sprouting resistance
in the substitution lines. Sequence analyses revealed a novel functional allele of qLTG3-1 in Nipponbare and a loss-of-function allele in Koshihikari. The allelic difference in qLTG3-1 between Nipponbare and Koshihikari is likely to be associated with differences in both pre-harvest sprouting resistance and
low-temperature germinability. 相似文献
19.
A. H. Price A. D. Tomos 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(1-2):143-152
Drought is a major abiotic stress of upland rice, and good root growth has been associated with drought avoidance. We report on the genetic mapping of root growth traits in an F2 population derived from two drought-resistant rice varieties, ‘Bala’ and ‘Azucena’. Restriction fragment length polymorphism (RFLP) between the parents was 32%, and a molecular map with 71 marker loci and 17 linkage groups covering 1280 cM was produced. Quantitative trait loci (QTLs) for eight root growth characteristics were mapped using phenotype data obtained in a hydroponic screen previously described in a companion paper. Using a significance threshold of LOD 2.4, we observed one QTL for maximum root length after 28 days growth on chromosome 11. It had a LOD score of 6.9, explained nearly 30% of the variation and appeared to be largely additive in effect. QTLs for maximum root length after 3, 7, 14 and 21 days of growth were also revealed. Some root-length QTLs, including that on chromosome 11, varied greatly with developmental stage. One QTL for root volume and two QTLs for adventitious root thickness were detected. No QTLs were detected for the length of cells in the mature (fully expanded) zone of adventitious root tips. The results obtained are discussed in the context of previous reports on mapping root growth parameters in rice. 相似文献
20.
To investigate the genetic factors underlying constitutive and adaptive root growth under different water-supply conditions, a double haploid (DH) population, derived from a cross between lowland rice variety IR64 and upland rice variety Azucena, with 284 molecular markers was used in cylindrical pot experiments. Several QTLs for seminal root length (SRL), adventitious root number (ARN) and total root dry weight (RW) respectively, under both flooding and upland conditions were detected. Two identical QTLs for SRL and RW were found under flooding and upland conditions. The relative parameters defined as the ratio of parameters under the two water-supply conditions were also used for QTL analysis. A comparative analysis among different genetic populations was performed for the QTLs for root traits and several consistent QTLs for root traits across genetic backgrounds were detected. Candidate genes for cell expansion and elongation were used for comparative mapping with the detected QTLs. Four cell wall-related expressed sequence tags (ESTs) for OsEXP2, OsEXP4, EXT and Xet were mapped on the intervals carrying the QTLs for root traits. 相似文献