血雉卵的人工孵化

2008～2010年,北京动物园在陕西洋县长青自然保护区采集血雉卵进行人工孵化研究。卵的保存及运输期为5～7d,卵保存温度17.14～18.66℃,湿度73.5%～87.1%,卵使用防震运输箱经34h运输后入孵,运输过程温度在16.38～25.95℃之间。孵化前使用碘氟稀释液擦拭卵表面。35枚受精卵中,19枚使用3台孵化机孵化,孵化温度37.4～37.5℃,使用14%～33%、40%～45%和60%～70%3个湿度梯度孵化;16枚卵使用乌鸡代亲孵化,袖珍温度记录仪测量乌鸡孵化温度在34.81～39.18℃之间,孵化环境湿度为23.4%～72.4%。记录孵化过程中的卵重量变化和孵化期。结果表明:研究期间血雉卵平均孵化率为76.5%,其中机器孵化率为84.2%,代亲孵化率为68.8%;平均孵化期为27d11h;卵失重率11.23%～16.54%,平均为13.77%±1.51%。     

四川宝兴圈养绿尾虹雉的人工孵化技术研究

在四川宝兴蜂桶寨绿尾虹雉研究中心开展绿尾虹雉Lophophorus lhuysii卵人工孵化研究,用不同湿度孵化2组卵:梯度湿度组使用3种湿度,使卵失重率趋于15%;均衡湿度组始终使用50%～55%的湿度。观察记录卵质量、胚胎发育情况。结果显示:成功孵化雏鸟12只,孵化率70.6%,雏鸟3个月成活率100%;卵孵化期28.1～30.2 d,平均(29.5±0.6) d,卵失重率10.7%～17.6%,平均(14.0±2.0)%,啄壳时间26.1～28.3 d,平均(27.4±0.6) d,出壳持续时间36.5～58.5 h,平均(48.7±6.8) h;卵的日平均质量与孵化天数极显著负相关;梯度湿度组和均衡湿度组在卵的失重率和初始雏重上的差异有统计学意义,而啄壳时间和卵孵化期的差异无统计学意义;卵失重率越小,初始雏重越大;与家鸡Gallus gallus domesticus卵胚胎的发育特征相似。保持卵失重率10%～15%可做为绿尾虹雉卵孵化湿度设置的参考。     

河北南大港黑翅长脚鹬繁殖习性观察

2001年3～10月,在河北省南大港湿地和鸟类自然保护区对黑翅长脚鹬(Himantopus himantopus)的繁殖习性做了研究。该鸟在本地区为夏候鸟,3月下旬迁来,9月中旬迁离,居留期约180d。栖息生境主要在盐场,平均种群遇见率为40．8只／km.平均窝卵数4．1枚,孵化期为20-21d,孵化率为89．7％,年繁殖力为1．86,孵化期卵平均失重率为16．0％,幼鸟为早成鸟。     

长山列岛发现黄嘴白鹭的繁殖种群

:1 999年 6～ 8月 ,在辽宁省长海县石城乡形人岛 (N30°31′,E1 2 3°0 2′)发现了目前已知国内最大的黄嘴白鹭繁殖种群 ,约 4 0 0余只。黄嘴白鹭于 4月份迁至该岛繁殖 ,巢材由蒿子杆构成 ,每窝产 4～ 6枚卵 ,卵为淡青色。 1 4枚卵平均大小为 4 8 5( 46 0～ 50 5)mm× 34 0 ( 31 0～ 36 5)mm ,平均重量为 2 8 3( 2 7 5～ 2 9 0 ) g。孵化期 2 1～ 2 3天     

人工巢箱条件下白眉姬鹟的繁殖参数

2005～2006年,在吉林省左家自然保护区的次生林中,对人工巢箱条件下白眉姬鹟(Ficedulazanthopygia)的繁殖参数开展了初步研究.结果表明,人工巢箱中自眉姬鹟的窝卵数为5～7枚,平均6.0枚;卵重平均为1.6 g.卵大小平均为17.0 mm×13.1 mm.孵化期平均为13.1 d,每巢平均出雏5.4只,育雏期平均为12.8 d,每巢平均出飞雏鸟5.3只.白眉姬鹟的营巢成功率为70.0%,繁殖成功率为81.3%.     

无锡太湖地区夜鹭及白鹭繁殖生物学研究

1999年 4～ 6月对分布在无锡地区的夜鹭 (Nycticoraxnycticorax)和白鹭 (Egrettagarzetta)的繁殖生物学进行了调查研究 ,调查地面积约 2 72 37 5m2 ,植被以马尾松 (Pinusmassoniana)和香樟树 (Cinnamomumhupehanum)为主。研究结果表明 :栖息地共有鹭巢近万个 ,平均密度为 0 36个 /m2 ,每棵树上平均巢数为1 0 6个 ,最多达 2 5个 /棵树 ;平均窝卵数夜鹭为 3 5 5枚 ,白鹭为 4 89枚 ;平均窝雏数夜鹭为 2 6 8只 ,白鹭为3 96只 ;孵化期白鹭为 1 9～ 2 1天 ,夜鹭为 2 2～ 2 3天 ;孵化成功率白鹭为 84 2 5 % ,夜鹭为 75 46 % ;白鹭雏鸟主要以小型鱼类和虾类为食 ,夜鹭雏鸟主要以鱼类和两栖类为食。     

文县疣螈繁殖初探

2006年4～9月、2007年4～9月,在四川青川初步观察了文县疣螈(Tylototriton wenxianensis)的繁殖习性,并研究了其主要特点,包括繁殖期雌雄差异、繁殖迁徙、交配与产卵、幼体发育等,同时在甘肃文县观察了其幼体的生长发育过程.结果表明,文县疣螈的繁殖期一般为4月上旬至9月上旬,繁殖期成体性比不断变化.该螈具有繁殖迁徙现象,迁徙及求偶行为由雄性占主动.求偶、交配及产卵均在水塘周围岸边的陆地上进行,产卵集中在5月初至7月末,雌螈平均产卵数为42.8枚(n=6),卵的平均孵化期为26.8 d(n=119).卵群的平均孵化率为46.3%(n=6).随着气温的升高、相对湿度和降水量的增加,雌螈产卵量和孵化率升高,卵的平均孵化期缩短.     

杂色山雀的繁殖成功率

2004～2006年的3～7月,在辽宁省白石砬子国家级自然保护区,对杂色山雀(Parus varius)的繁殖及繁殖成功率进行了初步研究。结果显示,杂色山雀主要营巢于海拔400～900m的阔叶杂木林、针叶林及林缘地带;繁殖期在3～7月,其洞巢种类多样,筑巢期约15d;巢为碗状,巢结构的2/3由苔藓构成;窝卵数为6～8枚,平均(6·92±0·92)枚(n=13);雌鸟单独孵卵,孵化期为(14·00±0·00)d(n=10);育雏由雌雄鸟共同承担,育雏期为(17·50±0·58)d(n=4)。杂色山雀繁殖成功率为50·95%,繁殖力为2·22。人为干扰是造成卵和雏鸟损失的主要原因,占总损失的74·19%。     

甘肃莲花山淡眉柳莺的繁殖记录及其孵卵行为

2001年6～7月,在甘肃省莲花山自然保护区记录了淡眉柳莺(Phylloscopus humei)繁殖,研究了其孵卵行为。结果表明,窝卵数为(4.3±0.5)枚(4～5,n=4),卵的大小为(14.0±0.4)mm×(11.2±0.4)mm(n=17)。育雏期为(13.8±0.5)d(13～14,n=4)。孵化期内雌鸟日活动期的平均长度为(833.2±40.0)min,平均每天离巢(46.6±12.2)次(n=27),每次离巢时间为(5.3±2.8)min(n=1435),每次在巢时间为(12.7±7.5)min(n=1409),在巢率为72.4%±11.6%(n=21)。雨天在巢和离巢的时间与晴天有显著差异,晴天在巢率为71.5%±12.1%(n=13),雨天在巢率为75.1%±9.0%(n=8)。在整个孵卵期间所有记录到的卵温平均为31.2℃(49066min内连续记录58879次)。雌鸟每次离巢,卵温平均下降(9.4±3.4)℃(n=1450)。     

广西防城发现黄嘴白鹭的繁殖种群

20 0 2年 3～ 8月 ,在广西防城万鹤山首次发现国家Ⅱ级重点保护动物黄嘴白鹭的繁殖种群 ,约 4巢 ,8只成鸟 1 4只雏鸟。黄嘴白鹭于该年 3月底 4月初迁来筑巢繁殖。巢材多为常绿、落叶阔叶树枯枝和松枝。窝卵数 4枚 ,卵为淡蓝青色。雌雄鸟轮流交替孵卵 ,孵化期 2 4 5 0± 1 5 0 ( 2 3～ 2 6)d ,孵化率1 0 0 %。育雏期 41± 2 ( 3 9～ 43 )d ,窝雏数 3 5 0± 0 2 9( 3～ 4)只 ,雏鸟成活率 87 5 0 %。食性主要为鱼( 77 84% )、虾 ( 1 6 76% )和青蛙 ( 5 40 % )。     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

鸡传染性法氏囊病病毒研究进展

传染性法氏囊病(Infection bursal disease, IBD)是由鸡传染性法氏囊病毒(Infectious bursal disease virus, IBDV)引起的鸡和火鸡的一种高度接触性传染病,给世界各国的禽养殖业带来了巨大损失.自IBDV发现至今新的变异株不断出现,分子结构的改变导致病毒致病力的改变及宿主对疫苗应答的改变,使得传统的疫苗已不能控制其流行,因此各国学者对其基因组结构和功能进行了广泛深入的研究,并积极研制新型有效的疫苗以达到防治的目的.     

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).     

Enterovirus 71 3C proteolytically processes the histone H3 N-terminal tail during infection

Highlights
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions.