不同硫取代度昆布多糖硫酸酯的合成及理化性质初步研究

目的:对昆布多糖进行不同硫取代度的硫酸酯化修饰,并对其产物的硫酸基含量、糖含量与分子量进行检测,为研究不同硫取代度昆布多糖硫酸酯的生物活性奠定物质基础。方法:采用氯磺酸-吡啶法对昆布多糖进行硫酸化修饰,通过改变硫酸化修饰条件,来制取不同硫酸基取代度的昆布多糖硫酸酯;利用盐酸水解-硫酸钡比浊法测定昆布多糖硫酸酯的硫酸基含量,并通过公式求得其硫取代度;用苯酚-硫酸法测定昆布多糖硫酸酯的多糖含量,并使用HPGPC法测定其分子量。结果:两种不同硫取代度昆布多糖硫酸酯的硫酸基含量分别为37.8%、45.92%,取代度分别为1.07、1.51,糖含量分别为44.52%、37.19%,分子量分别为13000、16000。结论:利用氯磺酸-吡啶法对昆布多糖进行硫酸酯化修饰,该方法可以获取不同取代度产物,酯化率高。     

薤白多糖的硫酸化修饰及体外抗氧化活性

为探讨薤白多糖硫酸化修饰的最佳条件,以及硫酸化修饰提高薤白多糖活性的可能性,采用氯磺酸-吡啶法对醇沉法得到的薤白多糖和柱层析纯化的3种分级薤白多糖进行硫酸化修饰,以氯磺酸-吡啶配比、反应温度和反应时间为自变量,修饰产物的硫酸基取代度(DS)为响应值,应用响应面设计法确定硫酸化修饰的最佳条件,用H2O2/Fe2+体系法和邻苯三酚自氧化法测定修饰产物的抗氧化活性。结果表明:薤白多糖氯磺酸-吡啶法修饰的最佳条件为氯磺酸∶吡啶=1∶3,反应温度65℃,反应时间2 h,此条件下硫酸根取代度为0.470,硫酸化修饰能提高薤白多糖的体外抗氧化活性。     

硫酸酯化川芎多糖的制备及其抗氧化活性研究

以三种川芎多糖组分和淀粉为原料采用氯磺酸-吡啶法制备硫酸酯化产物,紫外和红外光谱对其进行结构表征,氯化钡-明胶比浊法测定硫酸酯化川芎多糖的取代度,分别考察试样在硫酸酯化前后对邻苯三酚自氧化反应产生的超氧阴离子和1,1-二苯基-2-苦基肼(DPPH)自由基的体外清除率。实验结果表明:川芎粗多糖和硫酸酯化川芎多糖对两种自由基的清除作用远低于阳性对照维生素C,但硫酸酯化修饰有助于提升川芎多糖对DPPH自由基的清除作用,对超氧阴离子自由基无明显影响;硫酸基的引入能够提高淀粉对O2-.的清除作用,但清除作用很弱。     

香菇多糖的化学修饰及结构表征

为明确改性效果,本文选择适宜的方法对香菇多糖分子结构进行了化学修饰和结构表征.选用氯磺酸-吡啶法和浓硫酸法对香菇多糖硫酸酯化,并进行了比较,利用一氯乙酸法对其进行羧甲基化,借助红外光谱和核磁共振碳谱对样品分别进行了结构表征.结果表明,氯磺酸-吡啶法硫酸化取代度达1.38,产率达79.23%,硫含量达14.59%,效果优于浓硫酸法;1247和807 cm~(-1)的特征吸收峰证明了硫酸基的引入,碳谱δ 79.06附近的峰证明了C2和C4被部分硫酸基取代;羧甲基化碳谱中C2和C4位附近出峰说明其位上羟基被取代,且异头碳为单一β-D-吡喃环糖苷键构型.通过红外光谱和核磁共振碳谱对香菇多糖结构表征证实,硫酸酯化和羧甲基化二种化学修饰方法对香菇多糖分子结构改性是可行的,且氯磺酸-吡啶法更适合香菇多糖硫酸化.     

响应面法优化猴头菌硫酸化多糖

[目的]为了获得猴头菌多糖的最佳硫酸化修饰条件,提高猴头菌硫酸化多糖硫酸基团的取代度。[方法]采用单因素比较氯磺酸-吡啶摩尔比、反应温度和反应时间对猴头菌硫酸化多糖取代度的影响,利用响应面法对各因素的最佳水平以及各因素之间的交互作用进一步研究。[结果]猴头菌多糖的最佳硫酸化修饰条件为:氯磺酸-吡啶摩尔比为1:4、反应温度为59℃、反应时间为2. 6 h,取代度为0. 457,与模型的预测值基本相符。[结论]响应面法优化得到猴头菌多糖的硫酸化修饰条件参数准确,该模型可以用于猴头菌多糖硫酸化修饰条件的优化。     

响应面法探讨氯磺酸-吡啶法修饰条件对硫酸化当归多糖取代度的影响

用氯磺酸-吡啶法对当归多糖进行硫酸化修饰。用响应面法研究了修饰条件中反应温度(A)、反应时间(B)和氯磺酸-吡啶比例(C)三个因素对产物的硫酸基取代度的影响,建立回归模型,验证了其有效性,并分析了主效应和因素交互作用。结果表明,在A为65~95℃、B为60~180 m in、C为1:3~14.3范围内,三个因素与产物平均取代度(Y)的回归模型为Y=2.74+0.66×A+0.66×B+0.80×C+0.51×A×B-0.31×A×C-0.16×B×C-0.34×A2-0.23×B2-0.50×C2;F检验证明模型拟合较好,可以用于量化控制反应条件;三个因素对取代度的影响程度为CA=B,A和B之间存在极显著的交互作用。     

硫酸酯化剂和溶剂对海参岩藻聚糖硫酸酯化修饰的影响

比较了以N,N-二甲基甲酰胺(DMF)和吡啶(Py)两种溶剂,氯磺酸-吡啶(CA-Py)和三氧化硫-吡啶(SO3-Py)两种硫酸酯化剂对海参岩藻聚糖硫酸酯化的影响。结果表明:以CA-Py为酯化剂可使硫酸基的含量高达50%以上,以SO3-Py为酯化剂,硫酸基的含量仅达到20%左右;DMF为溶剂时可低温反应,Py为溶剂时反应温度必须90℃以上,但产品得率较高。红外光谱分析显示:硫酸酯化后,四种产品硫酸酯的特征吸收峰均显著增强。抗氧化活性实验结果表明:四种硫酸酯化产品对1,1-二苯基-苦肼基自由基的清除效果最好,对羟基自由基的清除效果次之,对超氧阴离子自由基的清除效果最小;以SO3-Py为酯化剂所得产品的抗氧化活性优于CA-Py为酯化剂的产品,DMF为溶剂的产品优于Py为溶剂的产品。因此,操作简便的SO3-Py法更适于海参岩藻聚糖的硫酸酯化。     

香菇多糖硫酸化衍生物的制备及其结构分析

采用改良的Ｗｏｌｆｒｏｍ方法制备了一系列的硫酸化香菇多糖衍生物。硫酸基含量测定结果表明,硫酸基的取代程序受反应时间和酯化试剂中氯磺酸与吡啶的比例的控制;证明甲基化分析方法不适合硫酸化香菇多糖衍生物的结构分析,＾１３Ｃ－ＮＭＲ数据表明,硫酸基取供在香菇多糖中Ｃ－６上,表明Ｃ－６位羟基的反应活性高于其他位置的羟基。     

昆布多糖硫酸酯化修饰及抗肿瘤活性的研究

对昆布多糖进行硫酸酯化修饰,考察修饰前后多糖结构及抗肿瘤活性的变化。采用氯磺酸-吡啶法进行多糖硫酸酯化修饰,考察了昆布多糖及其硫酸酯的红外光谱、核磁光谱特征,扫描电镜观察了表面形态,采用MTT比色法进行抗肿瘤活性评价。结果表明,昆布多糖及其硫酸酯都具有典型的多糖红外吸收,昆布多糖硫酸酯具有硫酸基的特征吸收峰;昆布多糖及其硫酸酯均是以β-(1→3)糖苷键为主链的多糖,昆布多糖硫酸酯的硫酸基取代位置在C2-OH与C6-OH。昆布多糖及其硫酸酯表面立体形态差异显著,昆布多糖表面呈云雾状或海绵状,昆布多糖硫酸酯表面呈片状或块状。昆布多糖及其硫酸酯对人肠癌细胞LOVO生长都具有明显的抑制作用,并且昆布多糖硫酸酯的抗肿瘤作用强于昆布多糖。     

魔芋低聚糖硫酸酯的制备及抗病毒活性研究

从酶解魔芋干粉中提取分子量在1500左右的葡甘露低聚糖Apkos,采用吡啶-氯磺酸法制备了它的硫酸酯衍生物S-Apkos,其硫含量为8.28%,取代度(Ds)为0.57.用Vero细胞和单纯疱疹病毒2型(HSV-2)考察硫酸酯化前后低聚糖的抗病毒活性.结果表明,本身无抗HSV-2活性的Apkos硫酸酯化后产生了抗病毒活性,500～4000μg/ml的SApkos能很好地抑制HSV-2引起的Vero细胞病变,而在500μg/ml及以上浓度条件下能完全抑制HSV-2在Vero细胞单层中形成病毒空斑.     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

鸡传染性法氏囊病病毒研究进展

传染性法氏囊病(Infection bursal disease, IBD)是由鸡传染性法氏囊病毒(Infectious bursal disease virus, IBDV)引起的鸡和火鸡的一种高度接触性传染病,给世界各国的禽养殖业带来了巨大损失.自IBDV发现至今新的变异株不断出现,分子结构的改变导致病毒致病力的改变及宿主对疫苗应答的改变,使得传统的疫苗已不能控制其流行,因此各国学者对其基因组结构和功能进行了广泛深入的研究,并积极研制新型有效的疫苗以达到防治的目的.     

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).     

Enterovirus 71 3C proteolytically processes the histone H3 N-terminal tail during infection

Highlights
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions.