榕树须活性部位的成分研究

采用硅胶柱层析色谱和重结晶方法对榕树须95%乙醇提取物进行分离,得到8化合物,通过理化性质和波谱技术分别鉴定为:α-香树脂醇乙酸酯(1),羽扇豆醇乙酸酯(2),木栓酮(3),木栓醇(4),正三十五烷醇(5),白桦酯酸(6),β-谷甾醇(7)和豆甾-4-烯-6β-羟基-3-酮(8)。除化合物6外,其余化合物均为首次从该植物中分离得到。     

白透骨消化学成分的研究

采用乙醇提取,硅胶柱反复层析的方法,从白透骨消[Glechoma biondiana (Diels) C.Y.Wu et C.Chen]全草的乙酸乙酯部分分离了11个化合物,利用现代波谱技术(MS,1H-NMR,13C-NMR,DEPT)以及与文献对照的方法鉴定了这些化合物的结构,分别为2α-羟基乌苏酸(1)、2α,3α-二羟基乌苏-12-烯-28-酸(2)、乌苏酸(3)、2α,3α,23-三羟基乌苏-12-烯-28-酸(4)、3β,24-二羟基乌苏-12-烯-28-酸(5)、3α-羟基乌苏-12-烯-28-酸(6)、豆甾烷-3,6-二酮(7)、7β-羟基谷甾醇(8)、豆甾醇(9)、β-胡萝卜苷(10)、棕榈酸(11).除化合物(3)和(10)外,其余均为首次从该植物中分离得到.     

贵州鼠尾草化学成分的研究

从贵州鼠尾草95%乙醇提取物分离得到8个化合物,通过理化性质及波谱方法分别鉴定为正三十三烷(1),十二烷酸十四烷酯(2),β-谷甾醇(3),胡萝卜苷(4),乌苏酸(5),2 a,3β-二羟基乌苏酸(6),白桦酸(7)和迷迭香酸(8)。以上化合物均首次从该植物中分离得到。     

中华小苦荬萜类化学成分的研究(英文)

从中华小苦荬全草的乙酸乙酯提取物中分离得到8个萜类化合物,通过波谱方法及文献对照分别鉴定为β-香树脂素(1),3β-羟基-20(30)-蒲公英甾烯(2),熊果-12-烯-3β-醇(3),羽扇豆醇(4),10-羟基艾里莫芬-7(11)-烯-12,8α-内酯(5),乌苏-12,20(30)-二烯-3β,28-二醇(6),3β,8α-二羟基-6β-当归酰基艾里莫芬-7(11)-烯-12,8β-内酯(7)和乌苏酸(8),化合物1～8均首次从该植物中分离得到。     

木棉根化学成分研究

为了解木棉(Bombax ceiba)的化学成分,从其根中分离鉴定了17个化合物,经理化性质和波谱分析,分别鉴定为24-烯-环阿尔廷酮(1)、环阿尔廷醇(2)、木栓酮(3)、羽扇豆醇(4)、3β-乙酰齐墩果酸(5)、齐墩果酸(6)、麦角甾-4,6,8(14),22E-四烯-3-酮(7)、胆甾-4-烯-3-酮(8)、5α,8α-环二氧-24-甲基-胆甾-6,9,22E-三烯-3β-醇(9)、β-谷甾醇(10)、6β-羟基-豆甾-4-烯-3-酮(11)、胆甾醇(12)、β-谷甾醇-3-O-β-D-葡萄糖苷-6′-O-二十烷酸酯(13)、亚油酸三甘油酯(14)、正三十一烷醇(15)、正二十二烷酸(16)、油酸月桂醇酯(17)。除化合物4、6、9、10外,其余化合物均为首次从该属植物中分离得到。     

马比木根的化学成分研究

通过硅胶、MCI和Sephadex LH-20反复柱层析、纯化,从马比木根的甲醇提取物中分离得到14个化合物,运用现代波谱技术鉴定为β-谷甾醇(1),β-胡萝卜苷(2),7-酮基-β-谷甾醇(3),β-sitosteryl-3-O-β-D-glucopyranoside-2'-O-palmitate(4),5α,6β-二羟基胡萝卜苷(5),羽扇豆醇(6),3-乙酰氧基-12-齐墩果烯-28醇(7),喜树碱(8),9-甲氧基喜树碱(9),10-羟基喜树碱(10),9-methoxy-mappicine-20-O-β-D-glucopyranoside(11),mappicine-20-O-β-D-glucopyranoside(12),(3S)-pumiloside(13),(-)-(3S)-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid(14)。化合物1~7、9~14为首次从该植物中分离得到。     

暖地大叶藓化学成分的研究

从真藓科大叶藓属植物暖地大叶藓Rhodobryum giganteum(schwaegr.)Par.的乙醇提取物中分离得到9个化合物,通过波谱分析鉴定其分别为麦角甾-7,22-双烯-3β,5α,6β-三醇(1),乌苏酸(2),琥珀酸(3),尿嘧啶(4),棕榈酸(5),槲皮素(6),碳二十九烷(7),β-谷甾醇(8),胡萝卜苷(9).化合物1～9均为首次从该植物中分离得到.     

钩藤化学成分的研究

从钩藤(Uncaria rhynchophylla(Miq.)Jacks.)带钩茎枝分离得到18个化合物,通过波谱学等方法鉴定为乌苏酸(1),27,28-二羧基乌苏酸(2),3β,6β,19α-三羟基乌苏酸(3),noreugenin(4),单棕榈酸甘油酯(5),3-酮-6β,19α-二羟基乌苏酸(6),sumresinolic(7),scopoletin(8),3β,6β,19α,24-四羟基乌苏酸(9),6-酮-3β,19α,23-三羟基齐墩果酸(10),3β,19α,24-三羟基乌苏酸(11),23-醛-3β,6β,19α-三羟基乌苏酸(12),钩藤苷元C(13),β-谷甾醇(14),胡萝卜苷(15),丁香酸(16),cleomiscosin D(17),cleomiscosin B(18).其中化合物4,7,9～11,17,18为首次从该植物中分离得到.     

硬毛地笋化学成分研究

采用各种色谱对硬毛地笋(Lycopus lucidus Turcz.var.hirtus Regel)的化学成分进行分离,通过理化性质和波谱分析进行结构鉴定。从硬毛地笋全草甲醇提取物中分离鉴定了9个化合物:β-胡萝卜素(1),7,3’,4’-三羟基黄酮(2),β-谷甾醇(3),3’,4’,5-三羟基-3,7-二甲氧基黄酮(4),α-香树脂醇(5),β-香树脂醇(6),24-羟基-乌苏-12-烯-28-酸(7),β-胡萝卜苷(8),24-甲基-5α-胆甾-7,22-二烯-6α-醇-3β-O-葡萄糖苷(9)。化合物4,7和9为首次从该科植物中分离得到,化合物1～9均为首次从硬毛地笋中分离得到。     

小花清风藤的化学成分研究

利用Sephadex LH-20,硅胶柱色谱和硅胶制备薄层色谱等分离方法反复分离纯化,从小花清风藤（Sabia parviflora Wall．ex Roxb）中分离得到9个化合物,通过波谱数据分析分别鉴定它们为二十五烷酸（1）、木栓酮（2）、5-氧阿朴菲碱（3）、3-氧化齐墩果酸甲酯（4）、齐墩果酸（5）、羽扇豆-20（29）-烯-3-酮（6）、羽扇豆醇（7）、β-谷甾醇（8）、脱镁叶绿甲酯酸（9）。化合物2,6、7、9首次从该植物中分离得到。     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Entwicklungsgeschichte und Ultrastruktur von Pollenkitt und Exine bei nahe verwandten entomophilen und anemophilen Angiospermensippen derAlismataceae,Liliaceae, Juncaceae,Cyperaceae, Poaceae undAraceae

Some closely related members of the monocotyledonous familiesAlismataceae, Liliaceae, Juncaceae, Cyperaceae, Poaceae andAraceae with variable modes of pollination (insect- and wind-pollination) were studied in relation to the ultrastructure of pollenkitt and exine (amount, consistency and distribution of pollenkitt on the surface of pollen grains). The character syndromes of pollen cementing in entomophilous, anemophilous and intermediate (ambophilous or amphiphilous) monocotyledons are the same in principal as in dicotyledons. Comparing present with former results one can summarize: 1) The pollenkitt is always produced in the same manner by the anther tapetum in all angiosperm sub-classes. 2) The variable stickiness of entomophilous and anemophilous pollen always depends on the particular distribution and consistency of the pollenkitt, but not its amount on the pollen surface. 3) The mostly dry and powdery pollen of anemophilous plants always contains a variable amount of inactive pollenkitt in its exine cavities. 4) A step-by step change of the pollen cementing syndrome can be observed from entomophily towards anemophily. 5) From the omnipresence of pollenkitt in all wind-pollinated angiosperms studied one can conclude that the ancestors of anemophilous angiosperms probably have been zoophilous (i.e. entomophilous) throughout.

     

Identification and Characterization of the First Equine Parainfluenza Virus 5

正Dear Editor,Parainfluenza virus 5 (PIV5), known as canine parainfluenza virus in the veterinary field, is a negative-sense,nonsegmented, single-stranded RNA virus belonging to the Paramyxoviridae family (Chen 2018). The virus was first reported in primary monkey kidney cells in 1954 (Hsiung1972), then it has been frequently discovered in various     

Pathogenic Characterization and Full Length Genome Sequence of a Reassortant Infectious Bursal Disease Virus Newly Isolated in Pakistan

<正>Dear Editor,Infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a nonenveloped virus belonging to the Birnaviridae family with a genome consisting of two segments of double-stranded RNA (segments A and B), targets B lymphocytes of bursa of Fabricious leading to immunosuppression. In Pakistan,poultry farming is the second biggest industry and IBD is the second biggest disease threating the poultry sector.However, there is limited genome information of IBDV