毛玉露的组织培养与快速繁殖

以毛玉露花茎子房为材料进行组织培养研究。结果表明：毛玉露启动与分化最适培养基为MS＋6-BA1mg／L＋KT1mg／L：继代与增殖培养基为MS＋6-BA0．5mg／L＋KT0．5mg／L＋NAA0．01mg／L,增殖倍率为10-20倍;复壮培养基为MS＋DPU（3,3’-二苯基脲）1mg／L＋NAA0．2mg／L,生根培养基为1／2MS＋NAA0．5mg／L,生根5条,生根率为100％。     

激素种类及其浓度对矮牵牛试管苗增殖及生根率的影响

以MS培养基为基本培养基,以矮牵牛试管苗为材料,并用不同浓度的细胞分裂素6-BA、KT、Ad分别与生长素NAA（0．10mg／L）进行配比试验,并用一定浓度的细胞分裂素6-BA、KT、Ad两两分别组合配比试验,探讨了不同浓度的细胞分裂素对矮牵牛试管苗的影响,以及两类生长素IBA和NAA对生根的影响。结果表明,适合于矮牵牛试管苗增殖的培养基有：（1）MS＋1．60mg／L 6-BA＋0．10mg／LNAA;（2）MS＋0．80mg／L 6-BA＋1．60mg／LKT＋0．10mg／L NAA;（3）MS＋0．80mg／L 6-BA＋0．20mg／LAd＋0．10mg／LNAA;（4）MS＋1．60mg／L KT＋0．20mg／L Ad＋0．10mg／L NAA。适合于矮牵牛试管苗生根的培养基有（1）1／2MS;（2）1／ZMS＋0．20mg／L1BA;（3）1／2MS＋0．20mg／LNAA。     

黄花小山菊的组织培养和快速繁殖

1植物名称黄花小山菊[Dendranthema hypargyrum（Diels）Ling et Shih]。 2材料类别茎尖。 3培养条件基本培养基为MS。丛生芽诱导和增殖培养基：MS＋6-BA1mg．L^-1（单位下同）＋NAA0．01：MS＋6-BA3＋NAA0．01：MS＋6-BA5＋NAA0．01。生根培养基：1／2MS＋NAA0．01;1／2MS＋NAA0．03;1／2MS＋NAA0．05。     

龙须海棠组织培养与试管内开花

1植物名称龙须海棠（Mesembryanthemum spectabileFlaw）。2材料类别种子。3培养条件种子萌发培养基：（1）1/2MS;增殖继代培养基：（2）MS＋6-BA2.0mg·L^-1（单位下同）＋NAA0.2;（3）MS＋6-BA1.0＋NAA0.5;（4）MS＋6-BA0.5＋NAA0.1;生根培养基：（5）1/2MS＋NAA0.1;（6）1/5NAA0．1;生根培养基：（5）1／2MS＋NAA0．1;（6）1／5MS＋NAA0．I＋PP3330．1。     

毛冬青的组织培养与快速繁殖

1植物名称 毛冬青（Ilex pubescens Hook．et Am．）。 2材料类别 成熟果实。 3培养条件 基本培养基为1／2MS。（1）种子无菌发芽培养基：1／2MS＋6-BA0．5mg．L-1（单位下同）＋IBA0．2;（2）丛芽增殖培养基：1／2MS＋6-BA1．0＋IBA0．2;（3）生根培养基：1／2MS＋6-BA1．0＋NAA0．5。     

啤酒花茎尖的组织培养和规模化生产

1植物名称 啤酒花（Humulus Iupulus Linn．）,品种‘余乐比特’。 2材料类 别茎尖。 3培养条件 以MS为基本培养基。（1）增殖培养基：MS＋6-BA0．01mg．L。（单位下同）＋IAA0．1;（2）茎尖生长培养基：MS＋6-BA3．0＋NAA0．2;（3）生根培养基：1／2MS＋IAA1．5。     

姜薯的组织培养与快速繁殖

1植物名称姜薯（Dioscorea alata L．）。 2材料类别块根。 3培养条件以MS为基本培养基。（1）诱导培养基：MS＋2,4-D2．0mg·L^-1（单位下同）＋6-BA0．5;（2）芽分化培养基：MS＋6-BA3．0＋NAA0．5;（3）生根培养基：1／2MS＋NAA0．5＋IBA0．5。     

金叶络石的组织培养与快速繁殖

1植物名称 金叶络石[Trachelospermum asiaticum（Sieb．＆Zucc．）NakaiCV．Ougonnishiki]。 2材料类别 茎段、顶芽。 3培养条件 以MS为基本培养基。（1）不定芽诱导培养基：MS＋6-BA3mg．L^-1（单位下同）＋NAA0．2：（2）增殖培养基：MS＋6-BA1．5＋NAA0．2;（3）生根培养基：1／2MS＋NAA0．2。     

雌雄栝楼的组织培养研究

对雌雄栝楼（Trlctmsanthes kirilowii Maxim）分别进行组织培养的研究结果表明：在雌雄栝楼组织培养过程中,愈伤组织的诱导和分化适宜的培养基均存在性别差异。愈伤组织诱导的适宜培养基分别是,雌性栝楼培养基为MS＋NAA0．5mg／L＋IBA0．5mg／L＋6-BA1．5mg／L;雄性栝楼培养基为MS＋NAA0．1mg/L＋IBA0．5mg／L＋6-BA0．5mg／L和MS＋NAA0．1mg／L＋IBA1．0mg／L＋6-BA1．0mg/L。愈伤组织分化的适宜培养基分别是,雌性栝楼培养基为MS＋NAA0．1mg／L＋IBA0．4mg／L＋6-BA1．2mg／L;雄性栝楼培养基为MS＋NAA0．3ms／L＋IBA0．2mg／L＋6-BA0．8mg／L;雌雄栝楼无根苗生根的适宜培养基均为MS＋NAA0．1mg／L。     

海芋的组织培养与快速繁殖

1植物名称海芋[Alocasia macrorrhiza（Linn．）Schott]。 2材料类别块茎上的不定芽。 3培养条件（1）无菌外植体培养基：MS;（2）诱导愈伤组织培养基：MS＋6-BA1．0mg．L^-1（单位下同）＋NAA0．1;（3）丛生芽诱导及增殖培养基：MS＋6-BA3．0＋NAA0．1＋椰乳100;（4）壮苗及生根培养基：1／2MS＋NAA0．1。     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Enterovirus 71 3C proteolytically processes the histone H3 N-terminal tail during infection

Highlights
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions.     

Detection of EBV and HHV6 in the Brain Tissue of Patients with Rasmussen’s Encephalitis

Rasmussen’s encephalitis (RE) is a rare pediatric neurological disorder, and the exact etiology is not clear. Viral infection may be involved in the pathogenesis of RE, but conflicting results have reported. In this study, we evaluated the expression of both Epstein-Barr virus (EBV) and human herpes virus (HHV) 6 antigens in brain sections from 30 patients with RE and 16 control individuals by immunohistochemistry. In the RE group, EBV and HHV6 antigens were detected in 56.7% (17/30) and 50% (15/30) of individuals, respectively. In contrast, no detectable EBV and HHV6 antigen expression was found in brain tissues of the control group. The co-expression of EBV and HHV6 was detected in 20.0% (6/30) of individuals. In particular, a 4-year-old boy had a typical clinical course, including a medical history of viral encephalitis, intractable epilepsy, and hemispheric atrophy. The co-expression of EBV and HHV6 was detected in neurons and astrocytes in the brain tissue, accompanied by a high frequency of CD8⁺ T cells. Our results suggest that EBV and HHV6 infection and the activation of CD8⁺ T cells are involved in the pathogenesis of RE.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).     

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans.