抗多重耐药病原菌的越南槐内生菌TRPH-35的抗菌活性及其鉴定

以大肠杆ATCC25922、金黄色葡萄球菌ATCC25923及其临床多重耐药分离株ETEC-AD9、MRSA-DF12共4株人体病原细菌为靶标菌,采用琼脂块法从越南槐内生真菌中筛选抗菌活性菌株,并以改良的微量肉汤稀释法测定其代谢产物的最小抑菌浓度;根据形态和分子特征鉴定拮抗菌株的分类学地位。结果表明,菌株TRPH-35的活菌块对各病原细菌的抑菌圈直径与阳性对照相当;其代谢产物对各病原菌的最小抑菌浓度为20~40μg/mL;初步鉴定该菌株为刺盘孢。本研究结果表明,菌株TRPH-35鉴定为刺盘孢,对参试病原细菌均显示强的抗菌活性,具有较大的开发应用潜力。     

山豆根化学成分研究

利用多种柱层析方法,从山豆根中分得5个化合物。经过理化及波谱分析,分别鉴定为L-广高丽槐素(1)、红车轴草苷(2)、槲皮素(3)、芦丁(4)和异鼠李素-3-芸香糖甙(5)。其中化合物3,5为首次从该植物中分离得到。关键词：山豆根;L-高丽槐素;红车轴草苷;槲皮素;芦丁;异鼠李素-3-芸香糖甙     

山豆根细胞毒活性成分研究

利用多种柱层析方法,从山豆根中分离得到四个生物碱,通过理化性质测定及波谱分析,分别鉴定为槐醇(1),13,14去氢槐醇(2),苦参碱(3)和氧化苦参碱(4),并首次利用MTT比色法测定了化合物1和2对体外培养的HL60及SMMC7721细胞增殖抑制率,同时分别考察了葛根素对二者增殖抑制试验的协同作用。     

蜘蛛抱蛋属（百合科）二新种

报道了在广西发现的2个百合科蜘蛛抱蛋属Aspidistra新种,即乳突蜘蛛抱蛋A. papillata G. Z. Li和环江蜘蛛抱蛋A. huanjiangensis G. Z. Li&Y. G. Wei。乳突蜘蛛抱蛋与大花蜘蛛抱蛋A. tonkinensis（Gagner. ）F. T. Wang&K. Y. Lang近缘。它们的共同特征是:花单生;花被裂片两侧彼此覆盖;柱头盾状,边缘3裂,上表面具3条辐射状沟缝。不同点是:乳突蜘蛛抱蛋植株较大花蜘蛛抱蛋的粗大;叶柄较粗;叶片椭圆形或长椭圆形;花被阔钟     

中国的红索藻属植物

记述了中国产红索藻属Thtorea Bory emend．Necchi的2种2变种,即棘刺红索藻T．hispida(Thorea)Desvaux emend,Sheath,Vis et Cole,棘刺红索藻小孢变种T．hispida var,microspora S．L．Xie et Z．X．Shi,蓝色红索藻T．vio—lacea Bory emend．Sheath,Vis et Cole和蓝色红索藻长毛变种T．violacea var．longipilosa S．L．Xie et Z．X．Shi．其中,蓝色红索藻T．violacea Bor yemend．Sheath,Vis et Cole为中国新记录,棘刺红索藻小孢变种T．hispida var．microspora S．L．Xie et Z．X．Shi和蓝色红索藻长毛变种T．violacea var,longipilosa S．L Xie et Z．X．Shi为新变种。     

值得注意的中国植物(续)

续报道了大戟科等 8个分类单位。大果留萼木 ( Blachia andamanica)为广东和海南的新记录 ;崖州留萼木 ( B.jatrophifolia)的新异名为 B.yaihsienensis;黄毛果巴豆 ( Croton lachynocarpusvar.kwangsiensis)是降级新组合 ;黄蓉花 ( Dalechampia bidentata)分布至云南西南部 ,D. bidentata var. yunnanensis为其新异名 ;海人树 ( Suriana maritima) ,广东省的东沙群岛才有 ,台湾不产 ;锥序丁公藤 ( Erycibe subspicata)其分布东缘在贵州西南部 ;头花小牵牛 ( Jacquemontia tamnifolia) ,广东的归化杂草 ;松下草 ( Petrosavissinii) ,广东新记录     

杓兰属(兰科)中一个国产种类的新等级

丽江杓兰大围山变种(Cypripedium lichiangense vat．daweishanense)被提升为种的等级：大围山杓兰(Cypripedium daweishanense)。此种的特征是：中萼片浅黄绿色,上面疏被栗色斑点;花瓣较短,近等长于或略长于唇瓣。而丽江杓兰(Cypripedium lichiangense)的中萼片为紫肝色;花瓣要长得多,大约为唇瓣长的2倍。它们均属于三棱组(Cypripedium sect．Trigonopedium)。该组共含11种,均为中国特有种。文中提供了一个该组的分种检索表。     

杓兰属(兰科)中一个国产种类的新等级(英文)

丽江杓兰大围山变种(Cypripedium lichiangensem var.daweishanense)被提升为种的等级:大围山杓兰(Cypripedium daweishanense)。此种的特征是:中萼片浅黄绿色,上面疏被栗色斑点;花瓣较短,近等长于或略长于唇瓣。而丽江杓兰(Cypripedium lichiangense)的中萼片为紫肝色;花瓣要长得多,大约为唇瓣长的2倍。它们均属于三组(Cypripediumsect.Trigonopedium)。该组共含11种,均为中国特有种。文中提供了一个该组的分种检索表。     

Pyrgophyllum,a New Genus of Zingiberaceae from China

Pyrgophyllum (Gagnep.) T. L. Wu et Z. Y. Chen is a monotypic genus of Zingiberaceae from China. Originally, it was placed in Kaempferia as a subgenus by Gagnepain (1901), later transferred to Camptandra by Schumann (1904) and to Caulokaempferia by R. M. Smith. (1972). In this paper, the authors suggest that the separation of Pyrgophyllum at generic rank is justified on cytological, morphological and anatomical evidence. It is characterised by the large lamina-like bracts, which are rarely seen in Zingiberaceae. The margin of each bract is adnate to the main axis of the inflorescence at the base and the lip is lobed. Trichomes unicellular, stomata each with 4-7 lateral subsidiary cells, lateral veins are of three various types of vascular bundles: girder-shaped, semigirder-shaped and isolated. The pollen grains are spinecent. The basic chromosome number (x) is 21. The type species, Pyrgophyllum yunnanensis (Gagnep.) T. L. Wu et Z. Y. Chen (Kaempferia yunnanensis Gagnep.), is only distributed in Yunnan and Sichuan provinces of China.     

木兰科植物订正

对部分国产木兰科种类进行了订正,归并7个新异名,澄清Mognolia odoratissima Law et R.Z.Zhou和Michelia sphaerantha C.Y.WuexLaw et Y.F.Wu两个种分类上的混乱。     

河南玉兰属两新变种

发表河南玉兰属两新变种,即：①狭被望春玉兰(Yulania biondii (Pamp.) D. L. Fu var. angustitepala D. L. Fu, T. B. Zhao et D. W. Zhao,var. nov.);②椭圆叶罗田玉兰(Y. pilocarpa (Z. Z. Zhao et Z. W. Xie) D. L. Fu var. ellipticifolia D. L. Fu, T. B. Zhao et J. Zhao,var. nov.)。     

水浸对越北腹露蝗卵的影响

越北腹露蝗Fruhstorferiola tonkinensis Will.是广东北江流域河滩蝗虫的优势种。以不浸水的蝗卵为对照,研究滞育期蝗卵浸水5～95d和胚胎发育期蝗卵浸水1～30d对卵孵化率和历期的影响。结果表明,随浸水时间的增加,孵化率下降,卵发育延迟;滞育期蝗卵经受95d的浸水仍有8.01%存活,而胚胎发育期蝗卵浸水30d后全部死亡,滞育期蝗卵比胚胎发育期蝗卵具有更强的耐浸水能力。说明越北腹露蝗以卵滞育的形式发展了对水浸胁迫的适应。     

Relationship of occurrence of Fruhstorferiola tonkinensis Will. and plant communities on riversides in northern Guangdong Province, China

Plant communities on riversides in northern Guangdong Province were investigated with all-survey method, where a kind of native grasshopper Fruhstorferiola tonkinensis Will. broke out severely. 108 plant species belonging to 43 families were identified, and some plant indexes in those plant communities including their name, average height, congregation index, total coverage rate, phenological phase and biod were noted. In addition, plant comparability, plant abundance, quantity character and attribute symbol of dominant species in plant communities on riversides were studied. The results showed that the arrangement and distribution of vegetations on riversides offered a favorable habitat for F. tonkinensis. Moreover, the density of first instar nymphs of F. tonkinensis in plant communities was calculated. The results of partial correlation analysis (PCA) about the relationship between plant communities and occurrence of F. tonkinensis showed that Pterocarya stenoptera C. DC. was the most palatable plant to F. tonkinensis, presenting a very significantly positive correlation between coverage rate of P. stenoptera and occurrence of F. tonkinensis. The partial correlation coefficient (PCC) was 0.856072 (F=32.92012, P=0.0001). Bambusa flexuosa was a non-preferable plant of F. tonkinensis, presenting a significantly negative correlation between them, and the partial correlation coefficient was ?0.607920 (F=0.16173, P=0.0211). Those results would help us to propose some approaches for the ecology control of F. tonkinensis on riversides in northern Guangdong Province.     

关于顶生金花茶分类地位的探讨

根据近年来对顶生金花茶和平果金花茶在其原产地的进一步调查和野外观察并参考过去的研究成果,发现:顶生金花茶与平果金花茶在分布、形态特征、物候期、生态习性及细胞学特性,尤其是繁殖器官和生活史等方面都有明显差异。因此,认为将其降为平果金花茶的变种颇为不妥,主张恢复顶生金花茶作为一个独立种的地位,其名称为顶生金花茶。     

关于蒙古绣线菊毛枝变种及回折绣线菊的学名订正

支持将蒙古绣线菊毛枝变种提升为种的等级且于宁夏绣线菊同种,但其合法学名应为Spiraea tomentulosa(Yu)Y.Z.Zhao。与此同时,本文发现了真正的蒙古绣线菊的毛枝变种--S. mongolica Maxim.var.pubescens Y.Z.Zhao et T.J.Wang。     

卧龙乌头的名实问题

发现卧龙乌头Aconitum wolongense W．T. Wang的模式实际上包含两种植物,即毛果甘青乌头A． tanguticum (Maxim．)Stapf．var．trichocarpum Hand.-Mazz．和展毛大渡乌头A．franchetii Fin.et Gagnep．var．villosum W．T. Wang,故将该种予以归并。     

Determination of quinolizidine alkaloids in Sophora tonkinensis by HPCE

A simple, rapid and reliable high-performance capillary electrophoresis method has been developed to determine quantitatively the alkaloid content of Sophora tonkinensis, a Chinese herb commonly known as shan-dou-gen. A total of seven quinolizidine alkaloids (cytisine, sophocarpine, matrine, lehmannine, sophoranol, oxymatrine and oxysophocarpine) could be readily separated within 15 min. The running buffer was 50 mM phosphate buffer (pH 2.5) containing 1% hydroxypropyl-beta-cyclodextrin and 3.3% isopropanol in water. The applied voltage was 25 kV, the capillary temperature was 25 degrees C, the detection wavelength was 200 nm and scopolamine butylbromide was used as internal standard. The method was used to analyse the chemical constituents of two commercial alternatives to shan-dou-gen. The alkaloid constituents of authentic shan-dou-gen gave a specific HPCE electropherogram that could be used to distinguish the drug from potential substitutes. Furthermore, the content of oxymatrine and the total content of the seven quinolizidine alkaloids could be used as quantitative markers in order to assess the quality of S. tonkinensis.     

On the Type of the Genus Yinshania (Cruciferae)

When the genus Yinshania Ma et Y. Z. Zhao was published, it had only a single species, Y. albiflora Ma et Y. Z. Zhao which was indicated as the type of the genus (Acta Phytotax. Sin. 1979). Y. Z. Zhao 155. was indicated as the type specimen of Y. albiflora. It is adequate to cite Y. albiflora Ma et Y. Z. Zhao as the type of genus Yinshania Ma et Y. Z. Zhao. In a revision (Acta Phytotax. Sin. 25(3): 204-219, 1987) Y. H. Zhang made a combintion, Yinshania acutangula (O. E. Schulz) Y. H. Zhang (=Cochlearia acutangula O. E. Schulz) and reduced Yinshania albiflora Ma et Y. Z. Zhao as a variety of Y. acutangula,i. e. Y. acutangula var. albiflora (Ma et Y. Z. Zhao) Y. H. Zhang. She is uncorrect, however, when she cited Y. acutangula (O. E. Schulz) Y. H . Zhang as the type of the genus Yinshania Ma et Y. Z. Zhao. It should be cited as follows: Yinshania Ma et Y. Z. Zhao Typus generis: Yinshania albiflora Ma et Y. Z. Zhao (=Yinshania acutangula(O. E. Schulz) Y. H. Zhang var. albiflora (Ma et Y. Z. Zhao) Y. H. Zhang)     

中国鹤顶兰属(兰科)一新记录种

报道了中国鹤顶兰属Phaius一新记录种——中越鹤顶兰P.tonkinensis(Aver.)Aver.。该种与紫花鹤顶兰P.mishmensis(Lindl.et Paxton)Rchb.f.相似,不同在于该种的花萼和花瓣均为象牙白色,唇盘的脊上无毛,脊两侧具稀疏白色长毛。该文提供了该新记录种的形态描述和墨线图。