共查询到20条相似文献,搜索用时 531 毫秒
1.
麦角菌原生质体用0.3mg/mL亚硝基胍(NTG)分别处理20,40,60和80min。随机挑取NTG处理后的原生质体再生单菌落共80个,分别接种到50mL离心管(内装10mL液体培养基)中,在24℃、220r/min摇床上培养12d。在紫外灯(254um)下选出46株荧光相对较强的培养物在24孔板上作Van Urk蓝色反应分析。挑选9株呈深蓝色反应的菌株作HPLC分析,其结果与24孔板法有明显的相关性。在麦角隐亭产率上,No.45最佳(来自NTG 80min剂量组),No.18、 No.26次之(来自N 相似文献
2.
碱性脂肪酶产生菌扩展青霉W—1382的诱变育种 总被引:2,自引:0,他引:2
以扩展青毒(Penicilumexpansum)S-14作为出发菌株,分别比较UV、Co60-γ射线、NTG单因子的诱变效应和以NTG、Co60-γ射线、UV+NTG作为诱变剂及自然分离在内四代的诱变育种。单因子诱变剂最适剂量分别为UV150s,Co60-γ射线48000rad,NTG300μg/ml80min,比较诱变效果表明:NTG最佳,Co60-γ射线次之,UV较差;连续三代诱变各代诱变剂及剂量分别为NTG300μg/ml100min,Co60-γ射线20000rad,UV30s+NTG150μg/ml,40min。复合处理比单因子处理效果好。实验中选育成功高产酶变株W-1382,结合自然分离和诱变育种,其酶活力较出发菌株提高了53.6%。 相似文献
3.
水稻与大黍不对称休细胞杂交再生植株 总被引:2,自引:0,他引:2
采用PEG(聚乙二醇)融合法,诱诱导水稻(Oryza sativa L.)原生质体与无融合生殖大黍(Panicum maximum Jacq.)原生质体融合,经过融合体筛选、培养,成功地获得了再生植株并移栽成活。在融合前水稻原生质体经过2.5mmol/L碘乙酰胺(IOA)在室温(22-25℃)条件下处理15min,大黍原生质体经过60Kr软X射线照射处理。对获得的28株融合再生植株进行初步检测发现 相似文献
4.
5.
石防风原生质体遗传转化及抗除草剂植株的再生 总被引:2,自引:0,他引:2
以石防风(Peucedanumterebinthaceum(Fisch.)Fisch.exTurcz.)叶柄愈伤组织为材料建立以致密细胞团为主的悬浮培养物,用酶解法获得原生质体。用PEG法将拟南芥菜(Arabidopsisthaliana(L.)Heynh.)抗除草剂基因导入原生质体后进行液体浅层培养。转化细胞经除草剂chlorsulfuron筛选,通过胚状体途径产生抗性小植株。转化处理106个原生质体,以加入40~50μg/mL质粒DNA,PEG终浓度为10%,于1mL转化介质中,26℃黑暗下处理20min的效果最好。ctDNA的加入对转化结果无明显影响。分子杂交试验表明,突变的als基因已整合进转化植株的基因组中。转基因植株的细胞在脱分化过程中仍具有抗chlorsulfuron的能力;移栽成活的转基因小苗仍表现出抗除草剂特性,而未转化的植株在低浓度除草剂下即逐渐死亡。表明转入的als基因在石防风细胞内能够稳定表达。 相似文献
6.
7.
本试验以普通小麦(Ta)、多花黑麦草(Lm)和多年生黑麦草(Lp)细胞悬浮系来源的原生质体为材料研究了碘乙酰胺(IOA)和若丹明(R—6G)对禾本科植物原生质休的失活效果。Ta、Lm和LP原生质体经1mmol/LIOA处理后存活率分另为34%。17%和0%,而对照的存活率分别为78%、68%和71%。使Ta和Lm原生质体全部失活的IOA浓度需2mmol/L。Ta、Lm和Lp原生质体经40μg/mlR—6G处理后的存活率分别为60%、6%和63%,而对照的存活率分别为70%、61%和87%。经40μg/mlR—6G处理后,Lm和LP原生质体植板率分别为0和13细胞团/皿而对照的植板率分别为598和399细胞团/皿。以上结果表明,IOA和R—6G都是有效的禾本科植物原生质体失活剂。 相似文献
8.
PEG法介导转化诸葛菜下胚轴原生质体获得转基因植株 总被引:3,自引:0,他引:3
采用诸葛菜无菌苗的下胚轴组织为材料,分离原生质体,在原生质体培养基中作液体浅层暗培养,植板率为5%,植株再生频率为100%。作者进而开展了遗传转化研究。为研究PEG介导转化诸葛菜原生质体的影响因素,通过瞬间表达,实验了PEG法转化子叶原生质体的过程,在此基础上将分离纯化后的原生质体与带HPT基因的质粒DNA(pBI222)混合,HPT基因作选择标记,PEG介导转化;重新收集转化后的原生质体,以5×104/ml的密度在原生质体培养基中作浅层培养;培养10—15天后用25mg/L的潮霉素(hygromycin)进行筛选,一月后出现少量细胞团,转入含潮霉素50mg/L的扩增培养基扩增愈伤组织,进而转入含50—100mg/L潮霉素的分化培养基诱导分化成苗,分化率为100%,转入生根培养基中生根成完整植株。抗性植株再生率为4×10(-5)。在获得再生转基因植株后,以再生植株叶片为材料,进行Southernblot分子杂交,证实外源基因已稳定整合到植物基因组中并表达,再生转基因植株频率为10(-5)。国内外首次转化诸葛菜属植物原生质体获得成功。 相似文献
9.
10.
菌根真菌——赭丝膜伞原生质体分离与再生研究 总被引:4,自引:0,他引:4
振荡培养48h的赭丝膜伞(Cortinariusrussus)菌丝体经0.5%巯基乙醇预处理后,离心法收集,以0.6mol/LMgSO4作高渗稳定剂,pH5.8,采用纤维素酶,离析酶和浸解酶联合处理,在三个温度(24℃,31℃35℃)下经11~16h反应均可获得较高的原生质体产量,最高产量达8×10^7个/g鲜重菌丝,分离得到的原生质体表现了快速的再生能力,在MMNC固体培养基上可达到20%以上的 相似文献
11.
12.
13.
正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases 相似文献
14.
Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme
responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare
the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show
that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by
distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of
demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least
one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of
the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable
potent antiproliferative synthetic drugs. 相似文献
15.
16.
The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle. 相似文献
17.
18.
H.-R. GREGORIUS 《Biological journal of the Linnean Society. Linnean Society of London》1984,23(2-3):157-165
For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献
19.
20.
Shen Jia-Yuan Li Man Xie Lyu Mao Jia-Rong Zhou Hong-Ning Wang Pei-Gang Jiang Jin-Yong An Jing 《中国病毒学》2021,36(1):145-148
正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016). 相似文献