应用意愿价值评估法评价河流生态恢复的时间稳定性——以上海城市内河为例

意愿价值评估法 (CVM)的评估结果是否具有时间稳定性是其可靠性检验中的重要问题,决定其能否应用于我国的生态系统服务价值评估.本文以上海城市内河生态恢复为评估对象,设计相隔1个月和2年的3次意愿价值评估方案,分别对3次调查的426、498和200份问卷进行了对比分析.结果表明： 3次支付意愿均值分别为14.2、14.1和18.0元,中位数分别为5、5和10元.进一步对支付意愿分布和主要统计值、影响因素、模型时间变量的显著性分析结果表明,相隔1个月的CVM 结果具有时间上的稳定性,而相隔2年的CVM 结果表现出一定差异.     

应用意愿价值评估法评价河流生态恢复的时间稳定性——以上海城市内河为例

意愿价值评估法 (CVM)的评估结果是否具有时间稳定性是其可靠性检验中的重要问题,决定其能否应用于我国的生态系统服务价值评估.本文以上海城市内河生态恢复为评估对象,设计相隔1个月和2年的3次意愿价值评估方案,分别对3次调查的426、498和200份问卷进行了对比分析.结果表明： 3次支付意愿均值分别为14.2、14.1和18.0元,中位数分别为5、5和10元.进一步对支付意愿分布和主要统计值、影响因素、模型时间变量的显著性分析结果表明,相隔1个月的CVM 结果具有时间上的稳定性,而相隔2年的CVM 结果表现出一定差异.     

城市河流生态系统服务的CVM估值及其偏差分析

城市河流具有特殊的景观生态学意义,并能提供重要的生态系统服务,其价值逐渐受到社会各界的广泛关注。以全国河道治理的样板工程——上海浦东张家浜综合整治为例,采用条件价值评估法(CVM),共回收507份有效二分式CVM调查问卷,研究了城市河流生态系统服务改善的支付意愿及其总经济价值,同时也重点对二分式CVM研究中的相关问题如问卷设计、偏差分析和CVM可靠性与有效性的计量经济学验证过程等进行了适量探讨。二分式CVM研究表明(1)张家浜生态系统服务改善的平均支付意愿为1289.08元/(a·户),张家浜作为一条城市河流在未来3a内每年为浦东新区提供的生态系统服务价值为RMB6.34亿元。这与国际上的研究结论在数量级上是接近的,但与国内研究结果相比明显较高,应与研究区域的经济水平差异有关。(2)二分式CVM问卷投标数额的设计较好地界定了支付意愿的范围和分布形态,支付意愿平均值具有较高的统计效率,问卷的设计调查过程较好地控制了CVM研究中一些常见的偏差。(3)收入、学历、捐款经历、环境态度等社会经济信息变量对支付意愿具有显著的影响水平,符合经济学理论,CVM的可靠性得到较好的验证。(4)58.10%的非张家浜沿线居民对张家浜生态系统服务的改善和保护具有正支付意愿,城市河流的非使用价值(存在价值)得到了良好体现,显示了条件价值评估法在估算生态系统服务非使用价值的有效性。     

基于条件价值法评估三亚海域生态系统多样性及物种多样性的维持服务价值

基于条件价值法(CVM)开展了三亚海域海洋保护区生态系统多样性和重要海洋物种多样性的维持服务价值评估。于2014年12月通过面对面调研的形式对三亚当地城镇居民进行问卷调查,建立了支付意愿(WTP)多元线性回归方程,再将此回归模型推广到海南全省城镇居民,从而评估了三亚海域海洋保护区生态系统多样性和重要海洋物种多样性的维持服务价值;同时也对CVM研究中的相关问题如问卷设计、偏差分析和CVM的可靠性与有效性等进行了验证和探讨。研究结果表明:(1)2014年三亚海域海洋保护区生态系统多样性及重要海洋物种多样性的维持服务总价值分别为3.734亿元和4.442亿元。(2)受教育程度和家庭人均年收入等社会经济信息变量对支付意愿具有显著的影响水平,符合经济学理论。(3) CVM作为陈述偏好性价值评估方法,评估结果受偏差的影响较大。为了尽可能减小偏差,需要从问卷的设计、调查员筛选培训、调查方式和被调查者等诸多方面严格把关。(4) CVM问卷支付数额的设计较好地界定了支付意愿的范围和分布形态,支付意愿具有较高的统计效率,CVM的可靠性得到较好的验证,显示了CVM评估法在估算生态系统服务非使用价值的有效性。鉴于居民的学历和收入在短时间内很难得到提升,建议相关海洋管理部门加强对三亚海域海洋保护区和海洋生物多样性的宣传教育,增强居民对三亚海洋保护区和海洋珍稀濒危生物的认知及保护意识。     

额济纳旗生态系统服务恢复价值评估方法的比较与应用

条件价值评估法是国际上衡量环境物品非利用经济价值的主要方法之一,其调查的问卷格式主要有开放式和封闭式两种类型。以黑河流域额济纳旗生态系统恢复为研究对象,在投标卡格式调查的基础上,以开放式、封闭式(单边界和双边界两分式)的问卷格式各设计了500份调查问卷,调查分析了黑河流域居民对额济纳旗生态系统服务恢复的支付意愿。分析结果表明,采用不同类型的调查方法得到的结论有较大差异,其中:开放式问卷的调查结果与投标卡式问卷的分析结果相差不大;封闭式问卷格式的调查结果是开放式问卷调查结果的3～4倍;同时,双边界两分式问卷比单边界两分式问卷更能逼近参与者的真实支付意愿。在综合比较多种不同的调查方法的基础上,认为以双边界两分式的评估结果作为额济纳旗生态系统服务恢复的总经济价值比较合适。因此,仅就黑河流域居民来说,用20a的时间将额济纳旗的生态系统恢复到20世纪80年代初的水平,其总经济价值的现值为3.674×108元。最后,讨论了条件价值评估的各种方法,并指出了今后研究中需加强的方面。     

黑河流域张掖地区生态系统服务恢复的条件价值评估

以当前国际上流行的衡量环境物品经济价值的重要方法——条件价值评估法 ( CVM) ,针对黑河流域张掖地区生态系统恶化的现状 ,以支付卡的方法设计了 70 0份调查问卷 ,调查了黑河流域居民对恢复张掖地区生态系统服务的支付意愿 ( WTP)。结果表明 ,黑河流域 96 .6 %的居民家庭对恢复张掖地区生态系统服务存在支付意愿。对支付卡数据的非参数估计结果表明 ,黑河流域居民家庭对恢复张掖地区生态系统服务的平均最大支付意愿每户每年在 4 5 .9～ 6 8.3元之间。支付卡数据的参数估计分析表明 ,黑河流域居民家庭对恢复张掖地区生态系统服务的平均最大支付意愿每户每年为5 3.35元。按黑河流域现有家庭数量计算 ,恢复张掖地区生态系统服务的经济效益每年至少在 2 2 4 6 .2 8× 1 0 4 元以上。由于恢复张掖地区生态系统服务具有巨大的正外部效应 ,因此 ,仅就黑河流域居民家庭数量估计的恢复张掖地区生态系统服务的经济价值 ,只是对恢复张掖地区生态系统服务的经济价值的最低估价     

北京市湿地生态系统非使用价值

非使用价值是生态系统的内在属性,科学合理地评价该价值对生态资源管理和合理配置具有重要意义.本文运用条件价值法(CVM法),通过调查北京市居民的支付意愿,定量评估北京市湿地生态系统的非使用价值.采用分区方式,根据分区的人口数确定发放调查问卷的数量,于2008年10月分别在城区和郊区随机发放600份和300份调查问卷.采用修正的单纯随机抽样统计分析模型,提出以家庭作为抽样统计单位的思想,分别对两分区反馈回的调查问卷进行统计分析,得到北京市湿地生态系统非使用价值的评估总值为每年2.65亿元,其中城区为每年1.99亿元,郊区为每年0.66亿元.分析社会特征与支付意愿和WTP值的相关性得出:教育程度和年龄是影响支付意愿和WTP值的主要因素;月收入在城区样本中对支付意愿和WTP值的影响很小,而在郊区样本中的影响则相当显著;性别对支付意愿和WTP值的影响不大.     

生态保护价值的距离衰减性——以三江平原湿地为例

应用二分式条件价值评估法对三江平原湿地生态保护价值进行定量评价。运用支付意愿函数模型构建双边界二分式CVM数据分析模型,应用生存分析中的参数回归模型建立支付意愿影响因素分析模型。建立具有距离变量及个人社会、经济属性变量的支付意愿函数,验证支付意愿具有"距离衰减性"。研究采用以面访调查为主、网上调查为辅的方式,进行支付意愿问卷调查,得出2010年三江平原湿地生态保护的人均支付意愿为134.582元/a,三江平原湿地的生态保护价值为33.412亿元/a。研究结论为政府相关政策的制定提供参考依据。     

城市生态系统休闲娱乐服务支付意愿及价值评估——以成都市温江区为例

城市生态系统为居民提供了供给服务、环境调节服务和休闲娱乐等精神文化服务。从现代消费选择理论出发,建立居民接受城市生态系统休闲娱乐服务支付意愿模型,采用Logit模型、Oprobit模型和实际调查数据,分析了居民对温江区城市生态系统休闲娱乐服务的支付意愿及影响因素,并运用CVM方法对其休闲娱乐服务价值进行了测算。结果表明:(1)居民支付意愿与支付数量在成都市区均低于温江区,两者支付意愿率分别为60.3%和69.1%,支付意愿值为127.1元/a和142.5元/a;(2)居民支付意愿和支付数量不仅是收入水平的体现,也是居民对城市生态系统内部基础设施服务和便利程度的主观评价,以及再次获取服务意愿等非经济因素的体现;居民受教育程度和再次获取服务的意愿对支付数量有负影响,这反映了人们对城市生态系统休闲娱乐服务认识与支付行为的偏差和"搭便车"的侥幸心理。(3)基于平均支付意愿,温江区城市生态系统休闲娱乐服务价值为4.1亿元,在城镇化不断推进和居民收入不断增加的背景下,城市生态系统具有较大的潜在休闲娱乐服务价值。最后,基于研究结论,提出了相应的政策建议。     

开封城市水域生态系统服务价值评估及影响因素分析

评估城市水域生态系统服务价值是城市生态调查的重要内容。以北方水城——开封市为研究对象,基于双边界二分式条件价值评估法评估受访者对城市水域生态系统服务的支付意愿。然后构建包含环境感知变量的Logistic模型,探究支付意愿的影响因素。结果表明：①73.17%的受访者对开封城市水域生态系统服务具有正支付意愿,支付意愿率较高。其中男性支付率高于女性,文化程度越高其支付率越高,居住于水域附近的本地居民支付率高于外地游客,曾有过捐款经历可以提高支付率。②对零支付样本区分,计算保留真实性零支付样本后受访者的平均支付意愿,结果为59.77元 户^-1 月^-1。③受访者的性别、家庭月收入、生态行为对支付意愿有显著影响。建议加强环保教育,为公众搭建参与城市生态建设的平台,提升公众生态行为,有利于提高城市水域生态系统服务价值。研究丰富了城市水域生态系统服务价值评估案例,可以为政府部门合理引导公众参与城市生态建设提供参考。     

Tuning of electronic properties of one-dimensional cyano-bridged Cu-Ni, Cu-Pd, and Cu-Pt bimetallic assemblies by stereochemistry of ligands

Preparations, XPS and electronic spectroscopy, and magnetism of seven new one-dimensional cyano-bridged coordination polymers, chiral [Cu(RR-chxn)₂][Pd(CN)₄] · 2H₂O (1), [Cu(trans-chxn)₂][M(CN)₄] · 2H₂O (2, 4, and 6 for M = Pd, Ni, and Pt), and [Cu(cis-chxn)₂][M(CN)₄] · 2H₂O (3, 5, and 7 for M = Pd, Ni, and Pt) (RR-chxn = cyclohexane-(1R,2R)-diamine, trans-chxn = racemic trans-cyclohexane-(1,2)-diamine, and cis-chxn = racemic cis-cyclohexane-(1,2)-diamine) have been reported in view of tuning of their electronic properties by stereochemistry of chxn ligands and metal-substitution. Comparison of Cu 2p_1/2 and 2p_3/2 peaks of XPS and broad d-d bands around 18 000 cm⁻¹ of electronic spectra are described systematically for 1-7. Variable-temperature magnetic measurement shows that complexes 1-7 indicate weak antiferromagnetic interactions via cyano-bridges. Because of semi-coordination coupled with pseudo Jahn-Teller elongation and electrostatic interaction for 1, the axial Cu-N coordination bond distances of 2.330(7) and 3.092(8) Å are considerably longer than those of equatorial ones in the range from 2.016(6) to 2.030(6) Å. The former bond distances of 1 are intermediate values among the related Ni (2.324(6) and 3.120(8) Å) and Pt (2.34(1) and 3.09(1) Å) complexes.     

Structure and function of S-adenosylhomocysteine hydrolase

In mammals, S-adenosylhomocysteine hydrolase (AdoHcyase) is the only known enzyme to catalyze the breakdown of S-adenosylhomocysteine (AdoHcy) to homocysteine and adenosine. AdoHcy is the product of all adenosylmethionine (AdoMet)-dependent biological transmethylations. These reactions have a wide range of products, and are common in all facets of biometabolism. As a product inhibitor, elevated levels of AdoHcy suppress AdoMet-dependent transmethylations. Thus, AdoHcyase is a regulator of biological transmethylation in general. The three-dimensional structure of AdoHcyase complexed with reduced nicotinamide adenine dinucleotide phosphate (NADH) and the inhibitor (1′R, 2′S, 3′R)-9-(2′,3′-dihyroxycyclopenten-1-yl)adenine (DHCeA) was solved by a combination of the crystallographic direct methods program, SnB, to determine the selenium atom substructure and by treating the multiwavelength anomalous diffraction data as a special case of multiple isomorphous replacement. The enzyme architecture resembles that observed for NAD-dependent dehydrogenases, with the catalytic domain and the cofactor binding domain each containing a modified Rossmann fold. The two domains form a deep active site cleft containing the cofactor and bound inhibitor molecule. A comparison of the inhibitor complex of the human enzyme and the structure of the rat enzyme, solved without inhibitor, suggests that a 17° rigid body movement of the catalytic domain occurs upon inhibitor/substrate binding.     

Influence of Fluoride on Rat Kidney Antioxidant System: Effects of Methionine and Vitamin E

The aim of the study has been to determine and compare the influence upon the kidney antioxidative system, exercised by administration of vitamin E, and vitamin E in combination with methionine, under conditions of oxidative stress induced by sodium fluoride. The experiment was carried out on Wistar FL rats (adult males) that, for 35 days, were administered water, NaF, NaF with vitamin E, or vitamin E with methionine (doses: 10 mg NaF/kg of body mass/24 h, 3 mg vitamin E per 10 μl per rat for 24 h, 2 mg methionine per rat for 24 h). The influence of administered sodium fluoride and antioxidants upon the antioxidative system in kidney was examined by analyzing the concentration of malondialdehyde (MDA) and the activity of the most important antioxidative enzymes (SOD, total and both its isoenzymes, GPX, GST, GR, and CAT). The studies carried out confirmed the disadvantageous effect of the administered dose of NaF upon the antixodiative system in rats (increase in the concentration MDA, decrease activity of all antioxidative enzymes). The administration of vitamin E increased the activity of studied enzymes with the exception of glutathione reductase GR; it also reduced the procesess of lipid peroxidation. It has been found that combined doses of vitamin E and methionine were most effective in inhibiting lipid peroxidation processes. The results confirmed the antioxidative properties of methionine.     

Reorientation of Microfibrils and Microtubules at the Outer Epidermal Wall of Maize Coleoptiles During Auxin-Mediated Growth

Auxin-mediated elongation growth of isolated subapical coleoptile segments of maize (Zea mays L.) is controlled by the extensibility of the outer cell wall of the outer epidermis (Kutschera et al., 1987). Here we investigate the hypothesis that auxin controls the extensibility of this wall by changing the orientation of newly deposited microfibrils through a corresponding change in the orientation of cortical microtubules. On the basis of electron micrographs it is shown that cessation of growth after removal of the endogenous source of auxin is correlated with a relative increase of longitudinally orientated microfibrils and microtubules at the inner wall surface. Conversely, reinduction of growth by exogenous auxin is correlated with a relative increase of transversely orientated microfibrils and microtubules at the inner wall surface. These changes can be detected 30–60 min after the removal and addition of auxin, respectively. The functional significance of directional changes of newly desposited wall microfibrils for the control of elongation growth is discussed.     

Land,livestock, and livelihoods: Changing dynamics of gender,caste, and ethnicity in a Nepalese Village

Over the past 10 years, Ghusel VDC, Lalitpur District has moved from primarily subsistence agriculture into the wider cash economy aided by the Small Farmers' Development Program (SFDP), which provides credit to farmers mainly for the purchase of buffalo for milk production, and by the National Dairy Corporation, which supports local dairy cooperatives. Analysis reveals that buffalo-keeping and milk sales are increasing the well-being of many households, while at the same time creating new inequalities in gender roles and responsibilities, greater inequities between Brahmin and Tamang residents in Ghusel, and placing pressures on the ecosystem for increased supplies of fodder and fuelwood. Evidence suggests that there is critical, need for attention to the social, and particularly gender-based, implications of maintaining livestock for milk sales and to the ecological underpinnings of this livelihood system.     

Chiral Separation of G‐type Chemical Warfare Nerve Agents via Analytical Supercritical Fluid Chromatography

Chemical warfare nerve agents (CWNAs) are extremely toxic organophosphorus compounds that contain a chiral phosphorus center. Undirected synthesis of G‐type CWNAs produces stereoisomers of tabun, sarin, soman, and cyclosarin (GA, GB, GD, and GF, respectively). Analytical‐scale methods were developed using a supercritical fluid chromatography (SFC) system in tandem with a mass spectrometer for the separation, quantitation, and isolation of individual stereoisomers of GA, GB, GD, and GF. Screening various chiral stationary phases (CSPs) for the capacity to provide full baseline separation of the CWNAs revealed that a Regis WhelkO1 (SS) column was capable of separating the enantiomers of GA, GB, and GF, with elution of the P(+) enantiomer preceding elution of the corresponding P(–) enantiomer; two WhelkO1 (SS) columns had to be connected in series to achieve complete baseline resolution. The four diastereomers of GD were also resolved using two tandem WhelkO1 (SS) columns, with complete baseline separation of the two P(+) epimers. A single WhelkO1 (RR) column with inverse stereochemistry resulted in baseline separation of the GD P(–) epimers. The analytical methods described can be scaled to allow isolation of individual stereoisomers to assist in screening and development of countermeasures to organophosphorus nerve agents. Chirality 26:817–824, 2014. © 2014 The Authors. Chirality published by John Wiley Periodicals, Inc.     

In vitro studies of the rabbit immune system. IV. Differential mitogen responses of isolated T and B cells.

The mitogenic responses of separated rabbit lymphocyte populations functionally analogous to mouse T and B cells have been tested in vitro. Purified T cells were prepared by passage over nylon wool (NW) and purified B cells prepared by treatment with antithymocyte serum and complement (ATS + C). ATS + C kills 70% of peripheral blood lymphocytes (PBL's) and 50% of the spleen cells while passage over NW yields 40% of the applied PBL's and 5–23% of the applied spleen cells. NW-purified T cells from the spleen or PBL's respond fully to concanavalin A (Con A) but have a reduced response to phytohemaglutinin (PHA) and little or no response to goat anti-rabbit immunoglobulin (anti-Ig). PBL's that survive ATS + C (B cells) are stimulated by anti-Ig but not by Con A or PHA. B cells purified from spleen do not respond to Con A or PHA but will respond to anti-Ig under appropriate conditions. A full spleen B-cell response to anti-Ig required removal of Ig produced by the cultures that blocked anti-Ig stimulation. It is concluded that, for rabbit lymphocytes, Con A and PHA are primarily T-cell mitogens and that anti-Ig is primarily a B-cell mitogen. However, the mitogen response of unfractionated PBL or spleen cell populations indicates an overlap in reactivity. This could be due to cells sharing T and B properties, alteration of cell populations by the fractionation procedures used, or recruitment of one population in the presence of a mitogenic response of the other population.     

邻苯二甲酸二丁酯对翡翠贻贝抗氧化酶及脂质过氧化水平的影响

实验室条件下,研究了不同浓度邻苯二甲酸二丁酯(DBP)长期胁迫(15 d)对翡翠贻贝内脏团和外套膜抗氧化酶(超氧化物歧化酶SOD、过氧化氢酶CAT)及脂质过氧化(LPO)水平(以MDA含量表示)的影响,以及受胁迫翡翠贻贝在清洁海水中恢复阶段上述生化指标的变化特征.结果表明:胁迫阶段,0.5和2.5 mg.L-1DBP下翡翠贻贝内脏团SOD活性表现为先抑制后逐渐恢复,12.5和62.5 mg.L-1下则持续受到显著抑制;不同浓度组CAT活性均明显被抑制.LPO水平明显升高.外套膜中,2.5 mg.L-1下SOD活性受到持续诱导,其他浓度组则先被抑制,后随曝露时间延长逐渐被诱导;各浓度组CAT的变化波动较大,没有明显规律;而LPO水平明显升高.净化恢复阶段,12.5和62.5 mg.L-1DBP胁迫下的内脏团SOD和CAT活性恢复较慢,其LPO水平随时间延长逐渐恢复至对照组水平;外套膜中SOD活性呈持续升高趋势,CAT活性和LPO水平则随时间延长恢复到对照组水平.     

多马胺能药物对鲇鱼促性腺激素（GtH）分泌活动的影响

以珠江流域鲇鱼（silurus asotus)为实验材料,研究了多巴胺（DA）能药物（DA及其D－2型受体拮抗物 ,DOM）对鲇鱼促性腺激素（GtH)释放的影响,结果表明,在性腺发育的各个时期,单独注射DOM（5ug/g)均不能显著提高鲇鱼血液基础GtH水平,当DOM与LHRH－A联合注射时能显著增强LHRH－A刺激GtH释放的作用;DA只能抑制GnRH诱导的GtH释放,对基础GtH释放无抑制作用,这种生殖内分泌调节方式与鲇形目的革胡子鲇（Clarias gariepinus)和大鳍Hu（Mystus macropterus)相似,而与鲤形目的鲁科（Cyrpindiae)鱼类不同。     

X-ray induction of dominant lethals in late spermatids and mature spermatozoa of Drosophila melanogaster: the role of oxygenation

The role of oxygenation in determining the sensitivity to the induction of dominant lethals was examined in late spermatids and mature spermatozoa of Drosophila melanogaster. 0–2-h-old or 7-day-old Oregon-K males were irradiated with different X-ray exposures in nitrogen, air or in oxygen and the frequencies of dominant lethals induced in these stages were studied. The results obtained confirm and extend Sobels' earlier observations and the interpretation derived therefrom namely, that under normal conditions in air, mature spermatozoa are characterised by a higher degree of oxygenation than late spermatids and this difference is sufficient to explain the differential response of these stages. Similar Oxygen-Enhancement-Ratios(OERs) (of about 2) were obtained for both the cell stages. The present data also revealed that late spermatids are significantly less sensitive than mature spermatozoa to the X-ray-induction of dominant lethals in a nitrogen atmosphere. A plausible mechanism is suggested to explain this observation.