Influence of Fluoride on Rat Kidney Antioxidant System: Effects of Methionine and Vitamin E |
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Authors: | Iwona B?aszczyk Ewa Grucka-Mamczar S?awomir Kasperczyk Ewa Birkner |
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Institution: | (1) Department of Biochemistry, Silesian Medical University, 19 Jordana Str., Zabrze, 41-808, Poland |
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Abstract: | The aim of the study has been to determine and compare the influence upon the kidney antioxidative system, exercised by administration
of vitamin E, and vitamin E in combination with methionine, under conditions of oxidative stress induced by sodium fluoride.
The experiment was carried out on Wistar FL rats (adult males) that, for 35 days, were administered water, NaF, NaF with vitamin
E, or vitamin E with methionine (doses: 10 mg NaF/kg of body mass/24 h, 3 mg vitamin E per 10 μl per rat for 24 h, 2 mg methionine
per rat for 24 h). The influence of administered sodium fluoride and antioxidants upon the antioxidative system in kidney
was examined by analyzing the concentration of malondialdehyde (MDA) and the activity of the most important antioxidative
enzymes (SOD, total and both its isoenzymes, GPX, GST, GR, and CAT). The studies carried out confirmed the disadvantageous
effect of the administered dose of NaF upon the antixodiative system in rats (increase in the concentration MDA, decrease
activity of all antioxidative enzymes). The administration of vitamin E increased the activity of studied enzymes with the
exception of glutathione reductase GR; it also reduced the procesess of lipid peroxidation. It has been found that combined
doses of vitamin E and methionine were most effective in inhibiting lipid peroxidation processes. The results confirmed the
antioxidative properties of methionine. |
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Keywords: | Sodium fluoride (NaF) Kidney Malondialdehyde (MDA) Superoxide dismutase (SOD) Glutathione (GSH) Glutathione peroxidase (GPX) Glutathione-S-transferase (GST) Glutathione reductase (GR) Catalase (CAT) |
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