麻叶绣线菊的组织培养与快速繁殖

1植物名称 麻叶绣线菊（Spiraea cantoniensis Lour．）。 2材料类别 茎段、叶片。 3培养条件 （1）芽诱导培养基：MS＋6-BA3．0mg·L^-1（单位下同）＋NAA0．1;（2）芽增殖培养基：MS＋6-BA2．0＋NAA0．1;（3）愈伤组织诱导培养基：MS＋6-BA1．0＋NAA0．5＋2,4-D0．5;     

青藏苔草的组织培养和快速繁殖

1植物名称 青藏苔草（Carex moorcroftii Falc ex Boott）。 2材料类别 种子。 3培养条件 （1）诱导培养基：MS＋NAA0．5mg·L^-1（单位下同）＋6-BA1＋2,4-D0．5;（2）继代培养基：MS＋NAA0．5＋6-BA0．5＋2,4-D2;（3）芽分化培养基：MS＋6-BAI＋NAA1;（4）生根培养基：1／2MS＋IAA3。     

正交设计优化彩色辣椒离体再生条件的研究

目的：优化彩色辣椒的离体再生条件。方法：以彩色辣椒无菌苗的幼叶、叶柄、茎段和茎尖为外植体进行愈伤组织的诱导,采用正交试验设计,研究6-BA、NAA和IBA对芽分化的影响,并进行了生根培养。结果：幼叶的愈伤组织诱导率高于其它外植体,在最适诱导培养基MS＋6-BA（2.0mg/L）＋NAA（0.2mg·L-1）上,达96.7%;筛选出高效芽分化的培养基为MS＋6-BA（3.0mg·L-1）＋NAA（0.5mg·L-1）＋IBA（0.1mg·L-1）,分化率达93.3%;适于生根的培养基为1/2MS＋NAA（0.2mg·L-1）,生根率达90%,根粗壮、较长,侧根多。结论：筛选出了彩色辣椒离体再生的适宜外植体和培养条件,为彩色辣椒离体快繁提供参考。     

6-BA对红掌组织培养中红叶变异的影响

以红掌盆栽品种‘Avo-Gloria’为试材,以MS＋0.2mg·L^-12,4-D为基本培养基,分别在添加1～10mg·L^-16-BA的10种脱分化培养基上,诱导其叶柄外植体产生愈伤组织;再以MS＋2mg·L^-16-BA＋0.2mg·L^-1 NAA为分化培养基诱导分化不定芽;以MS＋0.2mg·L^-1 NAA为生根培养基,从不定芽获得再生植株。结果显示：（1）在MS＋0.2mg·L^-12,4-D＋8～10mg·L^-16-BA的3种脱分化培养基上可产生9%～10%的绿色、质地较硬的愈伤组织;（2）愈伤组织在MS＋2mg·L^-16-BA＋0.2mg·L^-1 NAA的分化培养基上,经6～8次继代培养,可获得3%～7%的不定芽,并可生根长成再生植株;（3）再生植株定植3个月后,有3%～7%植株出现红叶变异,此红叶可终生表现为红色。     

寒兰的组织培养与试管开花

1植物名称 寒兰（Cymbidium kanran Makino）。 2材料类别 种子。 3培养条件 种子萌发培养基：（1）1／2B5＋6-BA0．6mg·L^-1（单位下同）＋NAA0．4;根状茎增殖培养基：（2）B5＋6-BA0．6＋NAA1．5;芽分化培养基：（3）B5＋6-BA1．0＋NAA0．2;生根培养基：（4）1／2B5＋NAA0．2;     

刺五加腋芽培养中抗褐化初探

研究刺五加腋芽分化生长过程中褐化现象的结果表明,以75％的酒精消毒40s,0．1％的升汞灭菌8min能较好地抑制褐化现象;1000mg·L^-1维生素C的抑制作用较为明显;WPM＋1．0mg·L^-16-BA＋0．1mg·L^-1NAA培养基的褐化现象最轻：15mg·L^-1蔗糖、5d暗处理和15d转瓶一次都能在一定程度上抑制褐化。     

大叶补血草的组织培养与快速繁殖

1植物名称 大叶补血草[Limonium gmelinii（Willd．）Kuntze]。 2材料类别 种子。 3培养条件 启动培养基：（1）MS。愈伤组织诱导与分化培养基：（2）MS＋BA0．3mg·L^-1（单位下同）＋NAA0．5;（3）MS＋BA0．3＋NAA1．0;（4）MS＋BA0．5＋NAA2．0;（5）MS＋NAA0．5;（6）MS＋2,4-D1.0;（7）MS＋BA1．0＋NAA0．5。     

松毛翠的组织培养和快速繁殖

1植物名称 松毛翠（Phyllodo cecaerulea L．Bab．）。 2材料类别 新萌发嫩茎尖。 3培养条件 基本培养基为MS。（1）嫩茎尖愈伤组织诱导培养基：1／3MS＋2-Ip5．0mg·L^-1（单位下同）＋NAA0．5＋3％蔗糖;（2）愈伤组织再分化培养基：1／3MS＋2-Ip4．5＋IAA0．4＋3％蔗糖;（3）芽的继代增殖培养基：1／3MS＋2-Ip3．5＋IAA0．25＋3％蔗糖;     

火龙果愈伤组织诱导与植株再生

为了建立火龙果愈伤组织诱导与植株再生体系,以火龙果茎段、幼苗和子叶为外植体进行离体培养试验。结果表明：茎段诱导愈伤组织的最优培养基为1／2MS＋2,4-D2．0mg·L^-1＋6-BAO．5mg·L^-1,诱导子叶愈伤组织的最适培养基是1／2MS＋2,4-D2．0mg·L^-1＋6-BA1．0mg·L^-1,诱导愈伤组织分化的最优培养基为1／2MS＋6-BA4．0mg·L^-1＋NAA0．5mg·L^-1,最佳生根培养基为1／2MS＋6．BA1mg·L^-1＋NAA0-3mg·L^-1。     

东南景天的组织培养和快速繁殖

1 植物名称 东南景天（Sedum alfredii Hance）. 　　2 材料类别 幼嫩叶片. 　　3 培养条件 基本培养基为MS.（1）愈伤组织诱导培养基：MS＋6-BA 0.1 mg·L-1（单位下同）＋2,4-D1.0～3.0;（2）愈伤组织继代培养基：MS＋6-BA 0.1＋2,4-D 1.0＋VC 2.0＋1.0 g·L^-1活性炭;（3）分化培养基：MS＋6-BA 2.0＋2,4-D 1.0＋AgNO4 2.0;（4）壮苗培养基：MS＋6-BA 0.1＋GA3 2.0;（5）生根培养基：1/3MS＋NAA 1.0.以上培养基中均加入12g·L^-1琼脂、30 g·L^-1蔗糖,pH 5.6～5.8,在智能型光照培养箱（MGC-300B型,上海一恒科技有限公司）内培养.光照时间16 h·d^-1,光照强度为40～60 μmol·m^-2·s^-1,培养温度为（25±1）℃.[第一段]     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Enterovirus 71 3C proteolytically processes the histone H3 N-terminal tail during infection

Highlights
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions.     

Detection of EBV and HHV6 in the Brain Tissue of Patients with Rasmussen’s Encephalitis

Rasmussen’s encephalitis (RE) is a rare pediatric neurological disorder, and the exact etiology is not clear. Viral infection may be involved in the pathogenesis of RE, but conflicting results have reported. In this study, we evaluated the expression of both Epstein-Barr virus (EBV) and human herpes virus (HHV) 6 antigens in brain sections from 30 patients with RE and 16 control individuals by immunohistochemistry. In the RE group, EBV and HHV6 antigens were detected in 56.7% (17/30) and 50% (15/30) of individuals, respectively. In contrast, no detectable EBV and HHV6 antigen expression was found in brain tissues of the control group. The co-expression of EBV and HHV6 was detected in 20.0% (6/30) of individuals. In particular, a 4-year-old boy had a typical clinical course, including a medical history of viral encephalitis, intractable epilepsy, and hemispheric atrophy. The co-expression of EBV and HHV6 was detected in neurons and astrocytes in the brain tissue, accompanied by a high frequency of CD8⁺ T cells. Our results suggest that EBV and HHV6 infection and the activation of CD8⁺ T cells are involved in the pathogenesis of RE.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).     

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans.