1998年以来长江经济带旅游生态安全时空格局演化及趋势预测

旅游生态安全是旅游业可持续发展的基础。基于DPSIR(驱动力-压力-状态-影响-响应,Driving force-Pressure-State-Impact-Response)模型,构建长江经济带旅游生态安全评价指标体系,综合采用熵权TOPSIS(逼近理想解排序法,Technique for Order Preference by Similarity to an Ideal Solution)法、空间变差模型、标准差椭圆模型、灰色动态模型等方法分析长江经济带1998—2017年旅游生态安全水平的时空动态演变特征,并对其未来空间分布格局进行合理预测。研究发现:(1)长江经济带旅游生态安全水平均值为0.305,综合指数由0.207上升至0.439,呈稳步提升态势,安全状态由较不安全级上升至临界安全级;(2)经济带旅游生态安全为不安全级和较不安全级的省区数量逐渐减少,而处于临界安全级的省区数量持续增加,整体正介于由中低级向高级过渡的关键阶段;(3)旅游生态安全空间变异程度不断增强,空间分异特征显著,整体呈现出"东部>西部>中部"的空间演变格局,同时低值区域具有明显的西南迁移现象;(4)旅游生态安全空间分布格局呈东北-西南走向,移动路径呈"西北→东北→东南"变化趋势,空间分布范围经历了"分散-集聚"的过程。预测结果显示:2018—2030年旅游生态安全重心将向东北方向移动,空间分布格局在东西和南北方向上均呈敛缩态势,空间溢出效应不明显。     

煤炭资源型城市生态安全评价——以锡林浩特市为例

煤炭资源型城市生态安全评价能够协调人口、资源和环境之间的矛盾,保障城市可持续发展。基于PSR模型,以煤炭资源型城市锡林浩特市为研究区域,从生态环境和社会经济方面共选取了33个指标因子,构建了煤炭资源型城市的生态安全评价指标体系,运用熵权TOPSIS法进行了城市生态安全综合评价,并通过障碍度模型对城市生态安全的主要障碍因子进行了研究。研究结果表明:(1)2008—2017年锡林浩特市生态安全水平总体呈缓慢上升趋势。生态安全综合评价指数从0.464上升到0.553,安全等级由较不安全转为临界安全状态;(2)10年间PSR三大指数表现出不同变化特征。压力指数呈下降后上升再小幅下降的变化趋势,状态指数呈波动上升趋势,响应指数呈快速上升趋势;(3)子系统的障碍度分析表明压力系统的障碍度最大,是影响煤炭资源型城市生态安全的首要因素。子系统的障碍度变化趋势表现为:压力系统和状态系统的障碍度均不同程度增大,响应系统的障碍度越来越小;(4)单项指标的障碍度分析表明影响该资源型城市生态安全的主要障碍因子集中在压力系统和状态系统,10年间最大障碍因子经历了从城镇化率到矿区面积的演变。     

内蒙古生态安全时空演变特征及驱动力

生态安全是平衡社会经济与自然环境协调发展的重要保障，评价生态安全时空演变及驱动因素，对区域生态安全格局构建及可持续发展意义重大。基于本土化参数的生态足迹模型对1990-2018年内蒙古地区各盟市生态足迹、生态盈亏及生态压力指数进行测算，采用熵权法构建综合指标体系，计算生态安全指数，评价区域生态安全及可持续发展状况，结合空间分析揭示其时空演化规律，并解析驱动因素。结果表明：1）研究期间内蒙古总体上呈"供不应求"的生态赤字状态，但生态承载增速大于生态足迹增速，2018年较1990年人均生态足迹和人均生态承载力分别提高了0.9-5.8倍和1.5-10.8倍，生态安全指数由大于5.0的不安全状态转变为小于4.0的安全状态，生态系统总体呈向好趋势发展。2）研究期间内蒙古地区生态足迹呈"东高西低"分布，生态承载力呈"中间高，两翼低"分布，生态安全重心向东北方向转移。3）经济水平、消费支出、产业结构是内蒙古地区生态安全及可持续发展的驱动因素，地理位置和气候因素是导致其空间差异的主导因子。     

九寨沟自然保护区生态安全动态评价及障碍因子

生态安全是国家安全体系的重要基石，自然保护区在涵养水源、维持生态平衡等方面发挥着重要作用，可有效缓解社会快速发展对自然环境的破坏，是维持区域生物多样性的重要安全屏障。九寨沟自然保护区地处生态脆弱敏感区，在自然灾害和人类活动叠加影响下的生态安全状况备受各界关注。基于此，采用PSR模型和障碍因子分析法，从资源环境、人类活动、社会经济等方面选取23项指标构建评价体系，综合评价九寨沟保护区20年来的生态安全动态变化，探明主要影响因素。研究表明：（1）2001-2019年九寨沟生态安全指数呈曲折上升趋势，从0.7933增加至0.8623，其中压力和状态安全指数呈波动下降趋势，响应指数呈逐年波动上升趋势。（2）总体而言，保护区处于"安全"状态，仅个别年份受地质灾害影响，降至"临界安全"水平，安全等级演变历程为"安全-临界安全-安全-临界安全-安全"。（3）保护区生态安全的障碍主要来自于响应层，主要障碍指标为保护区居民数年增长率（C2）、地质灾害发生次数（C6）和法律完善程度（C22），障碍度分别为12.63%、10.53%和9.47%。（4）随着灾害防治措施和管理体制的不断优化完善，最大障碍因子经历了由法律完善程度（C22）、污水处理设施完善程度（C20）和地质灾害防控措施完善程度（C17）到地质灾害发生次数（C6）、保护区居民数年增长率（C2）的变化。未来需加大环境保护投入力度、提升灾害防治技术和保护管理应对措施，进一步优化社区共管体制，探索自然保护与社区居民和谐可持续发展的科学路径，稳步提升九寨沟保护区生态安全水平。     

洞庭湖流域生态安全状态变化及其驱动力分析

通过构建由状态子系统、压力子系统和响应子系统组成的洞庭湖流域生态安全评价系统,重点研究了该流域的生态安全变化趋势,并采用因子分析方法探究其驱动力。结果表明,1949年以来,流域生态安全变化表现出如下特征:系统状态指数明显下降,波动性和阶段性显著;系统压力指数1990年后呈下降趋势;系统响应指数一直呈增长趋势;生态安全度自1990年以来开始稳步上升。因子分析表明,流域生态风险是自然和人为因素叠加的结果,农民人均收入增加、农业人口占总人口比例减少、单位面积土地产值和单位土地面积生态建设投入增加,是促进流域生态安全度提高的决定性因素。同时,本文提出了提高流域生态安全度的相关对策。     

基于改进TOPSIS-灰色GM(1,1)模型的张家界市旅游生态安全动态预警

旅游生态安全预警对旅游地协调生态环境保护与旅游产业快速发展的矛盾,推进区域社会经济持续健康发展具有重要意义.本文基于DPSIR模型,从驱动力、压力、状态、影响、响应5方面构建了张家界市旅游生态安全预警指标体系,在此基础上,运用改进TOPSIS法对2001—2014年张家界市的旅游生态安全警情格局进行评估,并运用灰色GM(1,1)模型对其2015—2020年的警情演变趋势进行预测.结果表明: 总体上,2001—2014年,张家界市的旅游生态安全贴近度呈略微上升态势,警度处于“中警”.就各子系统而言,2001—2014年,张家界市旅游生态安全驱动力系统和压力系统的警度呈上升趋势,由“轻警”演变成“重警”;状态系统和影响系统的警度变化不大,一直处于“中警”;响应系统的警度呈下降趋势,由“巨警”转变成“无警”.按现状发展态势,2015—2020年,张家界市的旅游生态安全贴近度将进一步上升,警度将由“中警”向“轻警”转变,但协调旅游发展与生态建设和环境保护关系的任务依然较艰巨.     

基于压力-状态-响应模型的黑河中游张掖市生态安全评价

生态系统服务的状态与生态是否安全密切相关，生态系统服务的正常发挥是实现生态安全的前提。近年来，中国西北荒漠-绿洲扩张速度加快，改变了生态系统服务的供给与传输，进而影响了区域生态安全。以典型荒漠-绿洲区域黑河中游的张掖市为例，基于压力-状态-响应模型，耦合生态系统服务理论，构建了黑河中游生态安全评价指标体系，评估了2001-2015年黑河中游生态安全演变趋势。研究结果表明黑河中游生态系统面临的资源消耗和环境污染压力越来越大，压力指数有所降低；部分地区出现植被退化、破碎化增加等现象，状态指数略有降低；在实施节水灌溉、产业转移、湿地保护等举措后，响应指数显著增加。总体来看，2001-2015年黑河中游生态安全水平呈现波动增长趋势，生态安全综合指数由0.3748增至0.5888，生态安全等级由"较不安全"达到"临界安全"状态，其中高效节水灌溉面积、生态用水量、地下水开采率等水资源相关要素是影响绿洲生态安全水平的主要因子。基于以上研究，提出了坚持生态保护优先、调整种植和产业结构、发展高效节水灌溉技术、提高公众生态保护意识等提高生态安全水平的相关对策与建议，以期为促进区域生态经济可持续发展提供支撑。     

普陀山岛旅游生态安全发展趋势预测

科学地预测海岛目的地旅游生态安全发展趋势,对促进海岛旅游经济和生态环境协调发展具有重要的理论意义和实践价值。基于可持续发展的视角,建立了由承载力-支持力-吸引力-延续力和发展力(CSAED模型)子系统构成的普陀山旅游生态安全指标体系,并在灰色系统GM(1,1)模型和RBF神经网络模型比较选优的基础上,对普陀山岛旅游生态安全发展趋势进行了预测。研究结果表明:(1)和灰色系统GM(1,1)模型相比,RBF神经网络模型的Pearson相关系数和误差均方根值更优,可更精确地拟合普陀山岛旅游生态安全发展趋势;(2)2015—2020年,普陀山岛旅游生态安全指数的RBF神经网络模型预测结果由0.7017增加至0.8135,安全等级由比较安全上升至非常安全。研究结果可为维护普陀山岛旅游生态安全提供决策依据。     

京津冀特大城市群生态安全格局时空演变特征及其影响因素

京津冀特大城市群的快速发展显著提升了地区生活水平,但也对生态环境造成了负面影响。以2000—2015年京津冀城市群面板数据为研究样本,建立"压力-状态-响应(PSR)"城市群生态安全协同会诊指标体系,采用TOPSIS和灰色关联组合方法计量其生态安全综合指数(Comprehensive Index of Ecological Security,CIES),运用因子分析法提取关键因素,并通过多元线性回归方法计算上述因素对生态安全的影响程度。结果表明:(1)京津冀城市群生态安全指数已经达到了预警状态,呈现"北高南低,西高东低"的空间格局,亟需建立三地协同联防联控的生态安全防治机制;(2)2000—2015年京津冀城市群的生态安全指数呈小幅波动状态,规划与政策对城市群生态安全指数具有显著影响效应;(3)13地市生态安全指数的演变趋势存在显著的差异,承德、廊坊仍呈下降趋势;(4)城镇化、社会发展以及技术进步对京津冀城市群生态安全具有显著的正相关性,开发水平、经济发展以及生态病理度则呈负相关性。由此,需要划定并严守城市群生态保护红线,控制空间开发强度与速度,以遏制生态恶化;完善并落实京津冀城市群协同发展的规划体系与相应的配套政策支撑体系,以保障城市群生态安全稳定提升;控制经济发展速度,提升社会发展水平,构建城市群一体化社会服务保障体系;提升生态技术研发水平,加大生态空间保护与修复,以降低生态安全风险,保障城市群生态安全格局。     

福建省生态环境与高质量发展耦合关系及驱动机制

研究生态环境与高质量发展耦合关系时空演变及驱动机制，对实现可持续发展、构建生态文明体系和建设美丽中国具有重要意义。综合构建了生态环境-高质量发展指标体系，运用耦合协调度模型、空间马尔科夫链和BP神经网络-地理加权回归组合模型，系统探讨2005-2020年福建省生态环境与高质量发展耦合关系的时空演变及驱动机制。结果表明：（1）研究期间，福建省生态环境指数整体稳定，而高质量发展指数呈现先降低后上升的变化趋势，二者在空间上的组合关系整体呈现错位特征。（2）福建省县域生态环境与高质量发展指标之间的耦合关系呈先降低后上升的趋势，且呈现明显的空间异质性。（3）协调发展和绿色发展是驱动生态环境质量提高的关键因素，其影响程度呈"圈层式"空间分布特征。     

六种藓类植物茎的比较解剖学研究

通过对6种藓类植物,即褶叶青藓(Brachythecium salebrosum(Web.et Mohr.)B.S.G.)、湿地匐灯藓(Plagiomnium acutum(Lindb.)Kop.)、侧枝匐灯藓(Plagiomnium maximoviczii(Lindb.)Kop.)、大凤尾藓(Fissidensnobilis Griff.)、大羽藓(Thuidium cymbifolium(Doz.et Molk.)B.S.G.)和大灰藓(Hypnum plumaeforme Wils.)嫩茎和老茎的石蜡切片和显微观察发现,同一藓类植株的嫩茎和老茎,茎结构稳定,不同种藓类植物茎横切面具有不同特征.植物体茎横切面形状、表层细胞的层数、细胞大小和细胞壁厚薄、皮层细胞大小和形状、中轴的有无以及比例等特征可以作为藓类植物的分科分类依据之一.     

Photoregulation of seed germination of wild-type and of an aurea-mutant of tomato

Seed germination of an aurea mutant of tomato ( Lycopersicon esculentum Mill.) is promoted by continuous irradiation with red, far-red or long-wavelength far-red (758 nm) light as well as by cyclic irradiations (5 min red or 5 min far-red/25 min darkness). Far-red light applied immediately after each red does not change the germination behaviour. Seed germination of the isogenic wild-type, cv. UC-105, is promoted by continuous and cyclic red light while it is inhibited by continuous and cyclic far-red light and by continious 758 nm irradiation. Far-red irradiation reverses almost completely the promoting effect of red light. The promoting effect (in the aurea mutant) and the inhibitory effect (in the wild-type) of continuous far-red light do not show photon fluence rate dependency above 20 nmol m⁻² s⁻¹. It is concluded that phytochrome controls tomato seed germination throgh low energy responses in both the wild type and the au mutant. The promoting effect of continuous and cyclic far-red light in the au mutant can be attributed to a greater sensitivity to P_fr.     

Cause of Senescence of Nine Sorts of Flowers

The levels of endogenous phytohormones and respiratory rate in nine sorts of flowers such as Cymbidium faberi Rolfe, Nopalxochia ackermannii Kunth and others were investigated both at full bloom and senescence and meanwhile the effect of exogenous phytohormones on prolonging the blossoms and promoting ethylene production were tested. There is a high content of endogenous ethylene in all the long-lived flowere, about 3–16 folds higer than the short-lived ones. There is a high level of ABA at full blooming flowers of short-lived flowers, in which there is no or only some cytokinins in it, but the ratio of CTK (6BA+zeatin)/ABA is smaller(l.7). The endogenous ABA reached a much higher level at senescence in all nine sorts of flowers, so it is reasonable to consider that it is ABA which plays an important role of regulation in controlling flower's senescence. There is a much higher level of GA3 and zeatin in the long-lived flowers which is not demonstrated in the shortlived ones. The respiratory rate is one of the factors controtling the longevity of flowers, but it does not play a decided role. Application of 6BA and zeatin prolongs distinctly orchid’s longevity, however exogenous IAA through the promotive action on ethylene production, evidently extends the longevity of the flowers of the Nopalxochia ackermannii Kunth.     

龙胆科药用植物化学成分的研究现状

龙胆科植物在我国的分布范围很广,且多数为药用植物,其多数种属的药用植物,至今其化学成分尚未被系统研究。综述了目前龙胆科药用植物的化学成分的研究现状及一般提取方法,对近年来发现的环烯醚萜及裂环烯醚萜类化合物进行了总结,为本科药用植物的更深入研究提供了参考。     

Interconnection of parameters of regulation system of lipid peroxidation and of morphophysiological parameters of mouse liver

A complex analysis of seasonal fluctuations of the mean group parameters of the system of regulation of lipid peroxidation has been performed in liver of Balb/c mice. Association of lipid characteristics and morphophysiological parameters is studied in the Balb/c mouse liver. An inter-connection is revealed between the liver index and the amount of lysoforms of phospholipids, the scale and character of the interconnection differing essentially depending on proportion of phos-phatidylcholine in mouse liver phospholipids.     

The effects of glutamine of the maintenance of embryogenic cultures of Cryptomeria japonica

Summary Embryogenic tissues of sugi (Cryptomeria japonica) were induced on a modified Campbell and Durzan (CD) medium containing 1 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 600 mg l⁻¹ glutamine, and subcultured in the medium of the same composition for over 1 yr. This resulted in a mixed culture of embryogenic and non-embryogenic cells. When embryogenic cells were isolated and cultured independently, their capacity to form embryogenic aggregates was lost. Thus, the non-embryogenic cells present within a mixed culture system were essential to the formation of embryogenic aggregates. When embryogenic tissues were isolated and cultured independently on a high glutamine-containing (2400 mg l⁻¹) medium, dry weights and endogenous levels of glutamine increased, and the tissue could generate a large number of embryogenic aggregates. Amino acid analysis of embryogenic and non-embryogenic cells from the maintenance culture indicated a higher level of glutamine was present in the latter. The high endogenous level of glutamine in the non-embryogenic portion of mixed cell masses may be the supplier of glutamine for maintaining the embryogenic property of the tissues.     

Specificity of action of piperidylcholine derivatives as substrates of cholinesterases of various origin

The review deals with study of enzymologic properties of a novel highly specific acetylcholinesterase substrate, N-(β-acetoxyethyl) piperidinium iodomethylate (“piperidylcholine”), and its 30 derivatives that were tested as effectors of cholinesterases of mammals and various species of Pacific squids. It was proven for the first time that responsible for specificity of action was structure of cyclic ammonium grouping of the alcohol part of molecule of the ester substrate. Analysis of specificity is performed based on enzymatic hydrolysis parameters—activity of catalytic center of cholinesterases and bimolecular constant of the reaction rate that are determined at optimal and low substrate concentrations. Among the specially synthesized group of thioester compounds there is revealed one more highly specific acetylcholinesterase substrate—N-(β-acetoxyethyl) piperidinium.     

Physiological characteristics of various species of strains of carboxydobacteria

Seven strains of aerobic carbon monoxide-oxidizing bacteria (carboxydebacteria) when growing on CO as sole source of carbon and energy had doubling times which ranged from 12–42 h. The activity profiles obtained after discontinuous sucrose density gradient centrifugation indicated that the CO-oxidizing enzymes are soluble and the hydrogenases are membrane-bound in all strains examined. The CO-oxidizing enzymes of Pseudomonas carboxydohydrogena, Pseudomonas carboxydoflava, Comamonas compransoris, and the so far unidentified strains OM2, OM3, and OM4 had a molecular weight of 230,000; that of Achromobacter carboxydus amounted to 170,000. The molecular weights of the CO-oxidizing and H₂-oxidizing enzymes turned out to be identical. The cell sonicates were shown to catalyze the oxidation of both CO and H₂ with methylene blue, thionine, phenazine methosulfate, toluylene blue, dichlorophenolindophenol, cytochrome c or ferricyanide as electron acceptors. Methyl viologen, benzyl viologen, FAD⁺, FMN⁺, and NAD(P)⁺ were not reduced. The spectrum of electron acceptors was identical for all strains tested. Neither free formate, hydrogen nor oxygen gas were involved in the CO-oxidation reaction. Methylene blue was reduced by CO at a 1:1 molar ratio. The results indicate that CO-oxidation by carboxydobacteria is catalyzed by identical or similar enzymes and that the reaction obeys the equation CO+H₂OCO₂+2H⁺+2e^- as previously shown for Pseudomonas carboxydovorans.Dedicated to Otto Kandler remembering almost three decades of enjoyable cooperation     

Modulation of allostery of pyruvate kinase by shifting of an ensemble of microstates

Since the introduction of the concepts of allostery about four decades ago, much advancement has been made in elucidating the structure-function correlation in allostery. However, there are still a number of issues that remain unresolved. In this review we used mammalian pyruvate kinase (PK) as a model system to understand the role of protein dynamics in modulating cooperativity. PK has a triosephosphate isomerase (TIM)(α/β)₈ barrel structural motif. PK is an ideal system to address basic questions regarding regulatory mechanisms about this common (α/β)₈ structural motif. The simplest model accounting for all of the solution thermodynamic and kinetic data on ligand-enzyme interactions involves two conformational states, inactive E^T and active E^R. These conformational states are represented by domain movements. Further studies provide the first evidence for a differential effect of ligand binding on the dynamics of the structural elements, not major secondary structural changes. These data are consistent with our model that allosteric regulation of PK is the consequence of perturbation of the distribution of an ensemble of states in which the inactive E^T and active E^R represent the two extreme end states. Sequence differences and ligands can modulate the distribution of states leading to alterations of functions. The future work includes: defining the network of functionally connected residues; elucidating the chemical principles governing the sequence differences which affect functions; and probing the nature of mutations on the stability of the secondary structural elements, which in turn modulate allostery.