腺苷A_1受体激动剂对兔血一氧化氮水平及急性心肌梗塞范围的影响

为了解腺苷Ａ１受体激动剂ＲＰＩＡ对血一氧化氮（ＮＯ）水平及急性心肌梗塞范围的影响,２０只新西兰兔随机分为四组：Ⅰ,前降支缺血６０ｍｉｎ,再灌注９０ｍｉｎ;Ⅱ,缺血前１５ｍｉｎ给予ＲＰＩＡ（０．３ｍｇ／ｋｇ）ｉｖ,余同Ⅰ组;Ⅲ,两次缺血１０ｍｉｎ,间隔１０ｍｉｎ然后缺血６０ｍｉｎ,再灌注９０ｍｉｎ;Ⅳ,缺血前１５ｍｉｎ给予选择性腺苷Ａ１受体拮抗剂ＤＰＣＰＸ（１．０ｍｇ／ｋｇ）ｉｖ余同Ⅲ组。分别测定缺血再灌注期血ＮＯ水平、心率、平均血压及ｄｐ／ｄｔ,实验结束经ＴＴＣ染色测定心肌梗塞范围。结果显示,ＲＰＩＡｉｖ后血ＮＯ水平迅速上升达基础值二倍,并在整个缺血期持续高于对照组,同时伴有心率（２６．３％）和平均血压（１７．３％）的下降,ｄｐ／ｄｔ测值各组无明显差异。心肌梗塞范围,Ⅰ．２３．１±９．４％,Ⅱ．１２．１±２．２％,Ⅲ．１１．４±３．０％,Ⅳ．２１．４±７．８％。结论：缺血预适应（ＩＰＣ）减少急性心肌梗塞范围,腺苷Ａ１受体激动剂可以达到ＩＰＣ效果,而该受体阻滞可以取消ＩＰＣ效果;血ＮＯ水平的升高有可能是ＲＰＩＡ引起ＩＰＣ效应的原因之一。     

黑龙江铜木蚜蝇雌性的描述

黑龙江铜木蚜蝇雌性的描述ＤＥＳＣＲＩＰＴＩＯＮＯＦＴＨＥＦＥＭＡＬＥＣＨＡＬＣＯＳＹＲＰＨＵＳ（ＸＹＬＯＴＯＭＩＭＡ）ＡＭＵＲＥＮＳＩＳ（ＳＴＡＣＫＥＬＢＥＲＧ）（ＤＩＰＴＥＲＡ：ＳＹＲＰＨＩＤＡＥ）￥ＨＥＪｉｌｏｎｇ;ＣＨＵＸｉｐｉｎｇ（Ｄｅｐａｒ...     

植物地理学的研究内容及趋势

植物地理学的研究内容及趋势应俊生（中国科学院植物研究所,北京１０００９３）ＣＯＮＴＥＮＴＳＡＮＤＴＲＥＮＤＳＩＮＰＨＹＴＯＧＥＯＧＲＡＰＨＩＣＡＬＳＴＵＤＩＥＳＹｉｎｇＴｓｕｎｓｈｅｎ（ＩｎｓｔｉｔｕｔｅｏｆＢｏｔａｎｙ,ＣｈｉｎｅｓｅＡｃａｄ...     

社鼠核型的研究

社鼠核型的研究ＫＡＲＹＯＴＹＰＥＳＯＦＮＩＶＩＶＥＮＴＥＲＣＯＮＦＵＣＩＡＮＵＳ（ＲＯＤＥＮＴＩＡ：ＭＵＲＩＤＡＥ）关键词核型Ｃ带Ｇ带ＡｇＮＯＲｓ社鼠ＫｅｙｗｏｒｄｓＫａｒｙｏｔｙｐｅ,Ｃｂａｎｄｉｎｇ,Ｇｂａｎｄｉｎｇ,ＡｇＮＯＲＳ,Ｎ...     

人铜锌超氧化物歧化酶cDNA的克隆,测序及表达

用逆转录聚合酶链反应（ＲＴＰＣＲ）,以人胎肝组织总ＲＮＡ为模板,扩增了人铜锌超氧化物歧化酶（ｈＣｕ,ＺｎＳＯＤ）的ｃＤＮＡ,并进行序列分析,将该ｈＣｕ,ＺｎＳＯＤｃＤＮＡ重组到Ｔ７启动子控制下的分泌型表达载体ｐＥＴ２２ｂ（＋）中,构建表达质粒ｐＥＴＳＯＤ,并转化大肠杆菌ＢＬ２１（ＤＥ３）。ＳＤＳＰＡＧＥ及蛋白质印迹分析表明,经１ｍｍｏｌ／Ｌ异丙基硫代βＤ半乳糖苷（ＩＰＴＧ）诱导后,可高效表达一分子量为１９ｋＤ的蛋白质,与抗人ＳＯＤ多抗有特异的免疫反应,表达量约为菌体总蛋白质的３０％,具有特异性ＳＯＤ酶活性,酶活力可达１７９７ｕ／ｍｌ培基。     

高氧预适应对大鼠心肌缺血损伤时抗氧化酶的影响

抗氧化酶具有减轻心肌缺血再灌注损伤的作用,在抗氧化酶中,比较重要的是超氧化物歧化酶（ＳＯＤ）,谷胱甘肽过氧化物酶（ＧｌｕｔａｔｈｉｏｎｅＰｅｒｏｘｉｄａｓｅ,ＧＳＨｐｘ）和过氧化氢酶（ＣＡＴ）。为了解高氧预适应（ＨｙｐｅｒｏｘｉｃＰｒｅｃｏｎｄｉｔｉｏｎｉｎｇ,ＨＯＰ）对大鼠心肌缺血损伤时抗氧化酶的影响,本实验将实验组大鼠放入高压氧舱内,每日吸８０－８５％氧气（１ａｔｍ,１５－２０％为氮气）６ｈ,连续７ｄ。利用Ｌａｎｇｅｎｄｏｒｆ装置做成心肌缺血再灌注模型。实验动物随机分为二个部分。第一部分可逆性心肌缺血（ＨＯＰＡ组与对照Ａ组）：缺血１０ｍｉｎ,再灌注６０ｍｉｎ。观察冠脉回流液中ＳＯＤ活力,检测心肌内抗氧化酶活力（ＳＯＤ,ＧＳＨｐｘ,ＣＡＴ）。第二部分不可逆性心肌缺血（ＨＯＰＢ组与对照Ｂ组）：缺血６０ｍｉｎ,再灌注６０ｍｉｎ。测定冠脉回流液中肌酸磷酸激酶（ＣＰＫ）含量,ＳＯＤ及心肌内抗氧化酶活力。结果表明：对于可逆性心肌缺血：ＳＯＤ,ＧＳＨｐｘ活力升高;对于不可逆性心肌缺血损伤：ＨＯＰ能减少ＣＰＫ释放,ＳＯＤ活力升高。     

蝽科—中国新纪录及印度篁蝽的补充描述（半翅目）

蝽科—中国新纪录及印度篁蝽的补充描述（半翅目）关键词中国，蝽科，新记录ＡＮＥＷＣＨＩＮＥＳＥＲＥＣＯＲＤＯＦＰＥＮＴＡＴＯＭＩＤＡＥＡＮＤＡＣＯＭＰＬＥＭＥＮＴＡＲＹＤＥＳＣＲＩＰＴＩＯＮＯＦＦｅｒｎｅｌｉｕｓｉｎｄｉｃｕｓＤＩＳＴＡＮＴ（Ｈｅｔｅｒ...     

用于测定树干木质部蒸腾液流的热脉冲技术研究概况

用于测定树干木质部蒸腾液流的热脉冲技术研究概况①李海涛陈灵芝（中国科学院北京森林生态系统定位研究站,北京１０００９３）ＡＩＮＴＲＯＤＵＣＴＩＯＮＴＯＴＨＥＨＥＡＴＰＵＬＳＥＴＥＣＨＮＩＱＵＥＡＮＤＩＴＳＡＰＰＬＩＣＡＴＩＯＮＴＯＭＥＡＳＵＲＩＮＧＴ...     

细叶菊挥发油的化学成分的研究

细叶菊挥发油的化学成分的研究安承熙（青海省测试计算中心,西宁８１０００８）ＳＴＵＤＩＥＳＯＮＴＨＥＣＨＥＭＩＣＡＬＣＯＮＳＴＩＴＵＥＮＴＳＯＦＥＳＳＥＮＴＩＡＬＯＩＬＳＯＦＡＪＡＮＩＡＴＡＮＵＩＦＯＬＩＡＡｎＣｈｅｎｇｘｉ（ＱｉｎｇｈａｉＰｒｏｖｉ...     

植物体的组件结构及其形态学和生态学意义

植物体的组件结构及其形态学和生态学意义叶万辉（中国科学院华南植物研究所,广州５１０６５０）ＴＨＥＭＯＲＰＨＯＬＯＧＩＣＡＬＡＮＤＥＣＯＬＯＧＩＣＡＬＳＩＧＮＩＦＩＣＡＮＣＥＯＦＰＬＡＮＴＭＯＤＵＬＡＲＣＯＮＳＴＲＵＣＴＩＯＮＹｅＷａｎｈｕｉ（Ｓｏｕ...     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Entwicklungsgeschichte und Ultrastruktur von Pollenkitt und Exine bei nahe verwandten entomophilen und anemophilen Angiospermensippen derAlismataceae,Liliaceae, Juncaceae,Cyperaceae, Poaceae undAraceae

Some closely related members of the monocotyledonous familiesAlismataceae, Liliaceae, Juncaceae, Cyperaceae, Poaceae andAraceae with variable modes of pollination (insect- and wind-pollination) were studied in relation to the ultrastructure of pollenkitt and exine (amount, consistency and distribution of pollenkitt on the surface of pollen grains). The character syndromes of pollen cementing in entomophilous, anemophilous and intermediate (ambophilous or amphiphilous) monocotyledons are the same in principal as in dicotyledons. Comparing present with former results one can summarize: 1) The pollenkitt is always produced in the same manner by the anther tapetum in all angiosperm sub-classes. 2) The variable stickiness of entomophilous and anemophilous pollen always depends on the particular distribution and consistency of the pollenkitt, but not its amount on the pollen surface. 3) The mostly dry and powdery pollen of anemophilous plants always contains a variable amount of inactive pollenkitt in its exine cavities. 4) A step-by step change of the pollen cementing syndrome can be observed from entomophily towards anemophily. 5) From the omnipresence of pollenkitt in all wind-pollinated angiosperms studied one can conclude that the ancestors of anemophilous angiosperms probably have been zoophilous (i.e. entomophilous) throughout.

     

Identification and Characterization of the First Equine Parainfluenza Virus 5

正Dear Editor,Parainfluenza virus 5 (PIV5), known as canine parainfluenza virus in the veterinary field, is a negative-sense,nonsegmented, single-stranded RNA virus belonging to the Paramyxoviridae family (Chen 2018). The virus was first reported in primary monkey kidney cells in 1954 (Hsiung1972), then it has been frequently discovered in various     

Pathogenic Characterization and Full Length Genome Sequence of a Reassortant Infectious Bursal Disease Virus Newly Isolated in Pakistan

<正>Dear Editor,Infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a nonenveloped virus belonging to the Birnaviridae family with a genome consisting of two segments of double-stranded RNA (segments A and B), targets B lymphocytes of bursa of Fabricious leading to immunosuppression. In Pakistan,poultry farming is the second biggest industry and IBD is the second biggest disease threating the poultry sector.However, there is limited genome information of IBDV