植物微核技术在监测水质污染中的应用

应用韭菜微核技术,检测哈尔滨市量具刃具厂含铬废水的遗传毒性,试验表明：含铬废水可诱导韭菜和蚕豆根尖细胞产生微核,进行回归分析,其蚕豆对Ｃｒ＾＋６诱变剂回归分析为ｙ＝２４．９４＋１２７．７７ｘ（ｒ＝０．９９６,Ｐ〈０．０１）,韭菜对Ｃｒ＾＋６诱变剂回归广泛为：ｙ＝２１．７４＋１００．２３ｘ（ｒ＝０．９００,Ｐ〈０．０１）,ｒ＝１１．１７＋３．３８ｘ（ｒ＝０．７８１,Ｐ〈０．０５）,韭菜根尖微核技术具     

不育男性精浆锌与其它精液参数的回归分析

对２２３例男性不育患者的精浆锌进行了测定,并用回归分析的方法研究了精浆锌与其它精液参数的关系．结果显示：精浆锌与精液ｐＨ、精子存活率之间存在显著的线性回归（Ｐ＜０．０１）,而精浆锌与精子密度、前向运动精子百分率、精子畸型率具有显著的曲线回归（Ｐ＜０．０１）．     

败血症大鼠肝细胞核Ca^2＋转运功能的改变

本实验观察败血症时肝细胞核钙转运的变化。早期败血症（结扎盲肠及穿刺后,９ｈ）大鼠肝细胞和肝细胞核钙含量分别增加２０％和３６％（Ｐ〈０．０５）。败血症大鼠肝细胞核Ｃａ＾２＋－ＡＴＰａｓｅ活性增加９４％（Ｐ〈０．０１）,核＾４５Ｃａ＾２＋转运显著增强（增加３２％,Ｐ〈０．０１）。核＾４５Ｃａ＾２＋转运与Ｃａ＾２＋－ＡＴＰａｓｅ活性呈明显正相关（ｒ＝０．９１４,Ｐ〈０．０１）。加入钙调素显著刺激而加入钙     

心衰心脏收缩蛋白分子变化及与收缩力的关系

采用ＤＯＣＡ硅胶管皮下埋入法建立大鼠心衰模型,从基因转录水平检测正常与心衰大鼠心肌组织中心肌收缩蛋白分子基因α－ＭＨＣ、β－ＭＨＣ、α－ｃａｒｄｉａｃａｃｔｉｎ、α－ｓｋｅｌｅｔａｌａｃｔｉｎ表达的变化。结果显示：（１）心衰大鼠心肌收缩力指标ｄｐ／ｄｔｍａｘ较正常大鼠明显降低（下降２７．５１％,Ｐ＜０．０１）,（２）心衰大鼠与正常大鼠相比,心肌组织中α－ＭＨＣ基因表达水平显著下降（降低２１．４３％,Ｐ＜０．０５）,β－ＭＨＣ基因表达水平显著升高（升高６２．４３％,Ｐ＜０．０１）、α－ｃａｒｄｉａｃａｃｔｉｎ基因和α－ｓｋｅｌｅｔａｌａｃｔｉｎ基因表达水平未见明显改变,（３）α－ＭＨＣｍＲＮＡ的含量与心肌收缩力指标ｄｐ／ｄｔｍａｘ值之间存在正相关关系（ｒ＝０．４１４３,ｎ＝４３,Ｐ＜０．０５）,β－ＭＨＣｍＲＮＡ的含量与ｄｐ／ｄｔｍａｘ值之间存在负相关关系。（ｒ＝－０．３９０２,ｎ＝４３,Ｐ＜０．０５）。这提示：心肌组织中ＭＨＣ基因表达水平的改变是心衰时心肌收缩力降低的主要分子基础     

ELISA 法用于马抗狂犬病毒抗体的检测

本文建立了ＥＬＩＳＡ间接法,用以检测精制（马）抗狂犬病血清（或血浆）制造过程中的中和抗体效价,并与传统的小鼠脑内中和法比较。结果表明（１）两种检测方法对不同水平抗体的检出是一致的。它们之间有很好的线性关系（ｙ＝２．１７ｘ＋２１,ｒ＝０．９９,ｐ＜０．０１）。（２）应用ＥＬＩＳＡ间接法测定马抗狂犬病血清效价简便、快速、特异性及重复性好,可代替小鼠脑内中和法。     

不育症精子乳酸脱氢酶同工酶C_4的细胞化学定位、定量研究

本文采用酶细胞化学方法,在光镜下用积分分级计数法,全自动图像分析仪检测精子ＬＤＨ－Ｃ４活性,两法测定ＬＤＨ－Ｃ４活性显著正相关（ｒ＝０．８０１和０．７４２,Ｐ＜０．０１）;ＬＤＨ－Ｃ４酶细胞化学电镜技术观察精子ＬＤＨ－Ｃ４活性,通过计数酶点可测定ＬＤＨ－Ｃ４活性。本文检查３８例不育男性和２０例已生育男性精子ＬＤＨ－Ｃ４活性,ＬＤＨ－Ｃ４主要分布于精子ＳＴＭ及胞质,其次是顶体及质膜表面。可通过胞膜外逸到精浆,ＬＤＨ－Ｃ４在各部活性比值为５∶２∶１∶１。不育组精子ＬＤＨ－Ｃ４可能通过膜破损处外漏到精浆使精子内ＬＤＨ－Ｃ４显著减少,ＬＤＨ－Ｃ４在精子顶体亦显著减少,使不育组精子ＬＤＨ－Ｃ４活性显著低于生育组精子,提示ＬＤＨ－Ｃ４活性的定位和大小直接与生育能力相关,检测ＬＤＨ－Ｃ４活性可作为检查不育症精子质量的可靠指标。ＬＤＨ－Ｃ４与精子密度显著相关（ｒ＝０．７３７和－０．４９３,Ｐ＜０．０５）;与活动性无相关性。     

植物中草酸积累与光呼吸惭醇酸代谢的关系

对几种Ｃ３和Ｃ４植物中草酸含量及相应的乙醇酸氧化酶活性测定结果表明,叶片光呼吸强度及其着急酶活性大小与草酸积累量没有相关性;植物根中均能积累草酸,但未测出乙醇酸氧化酶活性。烟草根、叶中的草酸含量在不同生长时期差异明显,且二者呈显著正相关（ｙ＝２．５６５ｌｎｘ＋２．１３７,ｒ＝０．７４９,Ｐ〈０．００１）,说明振中到可能来自叶片。氧化乙醇酸的瓣活性与氧化乙醛酸的酶的活性呈极显著线性正相关（ｙ＝０．２     

卵巢激素对肺泡巨洚细胞趋化活性的影响

为探讨卵巢激素对非性器官肺脏的防御功能有无影响,本研究以肺泡巨噬细胞（ＡＭ）趋化活必来指标,观察了卵巢激素对成年雄性大鼠离体ＡＭ趋化活性的作用。结果显示：不良浓度的酵母多糖激活血清与ＡＭ在体外培养３．５ｈ,对ＡＭ趋化性有良好的线性关系。雌二醇能抑制ＡＭ的趋化活性,量效关系显著（ｒ＝－０．９２８０,Ｐ＜０．０１）;而孕酮则促进ＡＭ的趋化活性,亦具有剂量依从性（ｒ＝０．９９７５,Ｐ＜０．０１）．ｒｊｆ     

脂质过氧化反应在家兔肝缺血再灌注损伤中的作用

同步观察１０只家兔肝缺血再灌注损伤（ＨＩＲＩ）时血脂质过氧化物的浓度、谷丙转氨酶活性及肝形态学的变化。发现脂质过氧化物浓度升高与谷丙转氨酶活性增高呈高度显著正相关（ｒ＝０．７３３,Ｐ＜０．０１）,并与肝损伤的严重程度呈平行关系。表明脂质过氧化反应在ＨＩＲＩ的发生、发展中起重要的作用     

丁胺卡那霉素对耳蜗外毛细胞运动及胞内游离钙的影响

本文运用连续录像手段和荧光测钙技术研究丁胺卡那霉素对离体耳蜗外毛细胞运动及胞内游离钙浓度的影响。结果显示：（１）正常状态下外毛细胞内游离钙的荧光比值为１．３９±０．０９（ｘ±ｓｘ,ｎ＝１５）;从等渗环境进入低渗环境外毛细胞变短变粗（Ｐ＜０．０５,ｎ＝７。（２）丁胺卡那霉素（６．６７ｍｇ／ｍｌ）可使外毛细胞胞膜皱缩内陷,局部直径明显变细（Ｐ＜０．０１,ｎ＝１９）,即使在低渗环境中也难以恢复原状。（３）丁胺卡那霉素（３．２３ｍｇ／ｍｌ）可瞬间降低细胞内钙离子浓度,使荧光比值降低１４．６８±２．０５％（Ｐ＜０．０１,ｎ＝１２）。提示丁胺卡那霉素的耳毒性作用机制可能通过降低细胞内游离钙浓度,进而影响外毛细胞钙依赖性的“慢能动性”运动     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

鸡传染性法氏囊病病毒研究进展

传染性法氏囊病(Infection bursal disease, IBD)是由鸡传染性法氏囊病毒(Infectious bursal disease virus, IBDV)引起的鸡和火鸡的一种高度接触性传染病,给世界各国的禽养殖业带来了巨大损失.自IBDV发现至今新的变异株不断出现,分子结构的改变导致病毒致病力的改变及宿主对疫苗应答的改变,使得传统的疫苗已不能控制其流行,因此各国学者对其基因组结构和功能进行了广泛深入的研究,并积极研制新型有效的疫苗以达到防治的目的.     

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).