异型兰的种子试管育苗

1植物名称异型兰(Chiloschista yunnanensisSchltr.)。2材料类别种子。3培养条件种子萌发培养基:(1)MS;(2)1/2MS(大量、微量元素用量减半);(3)3g·L-1花宝1号(美国Hyponex公司,N:P:K=7:6:19);(4)MS+100mL·L-1椰子乳;(5)1/2MS+100mL·L-1椰子乳;(6)3g·L-1花宝1号+100mL·L-1椰子乳;(7)1/2MS+100mL·L-1椰子乳+1g·L-1蛋白胨;(8)1/2MS+100mL·L-1椰子乳+1g·L-1胰蛋白胨;(9)1/2MS+100mL·L-1椰子乳+1g·L-1酵母提取液。生根育苗培养基:(10)3g·L-1花宝1号+2g·L-1活性炭+100g·L-1香蕉汁;(11)3g·L-1花宝1号+2g·L-1活性炭…     

多花脆兰的离体快速繁殖

1 植物名称多花脆兰[Acampe rigida(Buch.-Ham.ex J.E.Smith)P.F.Hunt]. 2 材料类别种子. 3 培养条件种子萌发培养基:(1)MS;(2)1/2MS(大量元素用量减半);(3)KC[成份如下:MgSO4·7H2O 250 mg·L-1(单位下同);KH2PO425O;(NH4)2SO4 500; Ca(NO 3)2·2H2O 1 000 ;FeSO4·7H2O 25; MnSO4.4H2O 7.5;肌醇(C6H12O6)100;盐酸硫胺素(VB,)1; D-甘露糖醇12.7%];(4)1 g·L-1花宝1号(美国Haponex公司产品,N:P:K=7:16:9)+2 g.L-1花宝2号(美国Haponex公司产品,N:P:K=20:20:20);(5)MS+100 g·L-1香蕉汁;(6)1/2MS+100 g·L-1香蕉汁;(7)KC+100 g·L-1香蕉汁;(8)1 g·L-1花宝1号+2 g·L-1花宝2号+100g·L-1香蕉汁.原球茎增殖和分化成苗培养基:(9)1/2MS+6.BA 1.5+NAA 0.2+100 g·L-1香蕉汁;(10)1/2MS+6-BA 0.5+NAA 0.05+100 g·L-1香蕉汁;(11)1 g·L-1花宝1号+2 g·L-1花宝2号+6-BA 1.0+NAA0.5+100 g·L-1香蕉汁.     

虎杖的组织培养与快速繁殖

以虎杖茎段、叶柄、叶片为外植体探讨了愈伤组织诱导、分化和植株再生的条件,筛选出茎段生长培养基为1/2MS+BA1.0mg·L-1+KT0.5mg·L-1+NAA0.2mg·L-1,茎段、叶柄和叶片外植体愈伤组织诱导培养基为MS+BA1.0～2.0mg·L-1+KT0.2～0.5mg·L-1+NAA0.2～0.5mg·L-1或MS+BA2.0～3.0mg·L-1+KT0.2～0.5mg·L-1+2,4-D0.5mg·L-1;丛生芽诱导培养基为MS+BA2.0mg·L-1+KT0.5mg·L-1+IBA0.2mg·L-1+LH1000;不定根及根状茎诱导培养基为1/2MS+IBA0.2mg·L-1.     

白旗兜兰种子非共生萌发与试管苗快速繁育

对我国极小种群野生植物白旗兜兰的种子非共生萌发与试管苗快速繁育开展研究,对比了不同培养基、预处理、种子成熟度及光照对种子萌发的影响。白旗兜兰种子萌发的最适条件是授粉后270 d的种子,在1/4MS+10%椰汁培养基上,用1%Na OCl溶液处理种子40 min并在播种后的前4周进行黑暗培养。最适合原球茎转化幼苗的培养基是3.0 g·L-1花宝一号+1.0 mg·L-1NAA+0.5 g·L-1活性炭+10%香蕉匀浆,而最佳幼苗生根壮苗的培养基是3.0 g·L-1花宝一号+1.0 mg·L-1NAA+1.0 mg·L-16-BA+0.5 g·L-1活性炭+10%香蕉匀浆。     

带叶兜兰的无菌播种和离体快速繁殖

1植物名称带叶兜兰(PaphiopedilumhirsutissimumPfitz)。2材料类别种子。3培养条件种子萌发培养基:(1)RE(Arditti1982);(2)VW;(3)1/4MS;(4)MS;(5)RE+活性炭2g·L-1;(6)RE;(7)RE+活性炭2g·L-1;(8)花宝1号(美国Haponex公司产品,N∶P∶K=7∶6∶19)2.5g·L-1。原球茎增殖和分化成苗培养基:(9)RE+6-BA2.0mg·L-1(单位下同)+NAA0.2;(10)RE+活性炭2g·L-1+6-BA2.0+NAA0.2;(11)花宝1号1.5g·L-1+花宝2号(美国Haponex公司产品,N∶P∶K=20∶20∶20)1.5g·L-1+活性炭2g·L-1+6-BA2.0+NAA0.2。生根壮苗培养基:(12)RE+NAA1.0+活…     

蒙花忍冬的组织培养与快速繁殖研究

以金银花 蒙花 品系的茎段为外植体 ,于 MS和 B5附加不同激素配比的培养基上 ,探讨愈伤组织形成和丛生芽诱导及生根培养的条件 .诱导愈伤组织和丛生芽的培养基为 B5+BA2 .0 m g· L- 1 +KT0 .2～ 0 .5 mg· L- 1+IAA 0 .5～ 1 .0 mg· L- 1 +L H 1 0 0 0 m g· L- 1 ,继代增殖培养的培养基为 B5+BA 2 .0 m g· L- 1 +KT 0 .5 m g·L- 1 +IAA1 .0 m g· L- 1 +L H1 0 0 0 m g· L- 1和 B5+BA2 .0 mg· L- 1 +KT0 .36 m g· L- 1 +IAA1 .0 mg· L- 1+CH 1 0 0 0 m g· L- 1 +1 / 2 MS :大量元素 ,生根培养基为 1 / 2 MS +IBA 0 .2 mg· L- 1 +L H 1 0 0 0 mg·L- 1 .结果表明 L H和 CH在金银花愈伤组织和丛生芽诱导方面具明显的作用     

细茎石斛的快速繁殖和试管开花诱导

1植物名称细茎石斛[Dendrobiummoniliforme(L.)Sw.]。2材料类别野生植株的成熟种子。3培养条件种子萌发培养基:(1)1/2MS+NAA0.5mg·L-1(单位下同);增殖分化培养基:(2)1/2MS+NAA0.4;生根壮苗培养基:(3)1/2MS+NAA0.2+6-BA2.0;试管开花预处理培养基:(4)1/2MS+TDZ0.05,(5)1/2MS+PP3330.40+ABA1.0,预处理90d;试管开花培养基:(6)MS(1/6N,2P,2K)+PP3331.0+TDZ0.1。每个处理20株,重复3次,150d内统计花芽诱导率(花芽数/植株数)和正常花开放率(正常花开放数/植株数)。以上培养基均附加4%蔗糖、10%(W/V)香蕉汁、8g·L-1琼脂、0.1g·…     

土人参茎尖培养和植株再生

1　植物名称　土人参 (Talinumpaniculatum)。2　材料类别　实生苗之茎尖 ,种子取自本校花圃。3　培养条件　种子萌发培养基 :( 1 ) 1 /2MS +0 .7%琼脂 + 2 %蔗糖。丛生芽诱导培养基 :( 2 )MS + 6 BA 0 .5mg·L- 1 (单位下同 ) ;( 3)MS +NAA0 .5 + 6 BA 2 .0 ;( 4 )MS +NAA 0 .5 +KT 2 .0 ;( 5 )MS+NAA 0 .5 + 6 BA 3.0。丛生芽增殖培养基 :( 6)MS + 6 BA 2 .0 ;( 7)MS +NAA 0 .0 5 + 6 BA 3.0。生根培养基 :( 8) 1 /2MS +NAA 2 .0。上述 ( 2 )～ ( 8)培养基均附加 30 g·L- 1 蔗糖、7g·L- 1 琼脂 ,pH 5 .8。培养室温…     

火焰兰的种子培养和试管育苗

1植物名称火焰兰(Renanthera CoCCinea). 2材料类别种子. 3培养条件种子萌发培养基:(1)VW;(2)VW 100 mL·L-1椰子乳;(3)KC;(4)KC 100 mL·L-1椰子乳(5)1/2MS;(6)MS.生根育苗培养基:(7)3 g·L-1花宝1号(美国Haponex公司产品,N:P:K=7:6:19) 2 g·L-1蛋白胨 2 g·L-1活性炭 0.5 mg·L-1NAA 0.2 mg·L-16-BA;(8)1 g·L-1花宝1号 1 g·L-1花宝2号(N:P:K=20:20:20) 2 g·L-1蛋白胨 2 g·L-1活性炭 0.5 mg·L-1NAA 0.2 mg·L-16-BA.以上培养基均附加1.5%蔗糖、0.6%琼脂,pH 5.2～5.4.培养温度为(25±2)℃,光照度1 500～2000lx,光照时间12 h·d-1.     

羚羊石斛的离体快速繁殖

1植物名称羚羊石斛(DendrobiumcochliodesSchltr.)。2材料类别种子。3培养条件萌发培养基:(1)VW;(2)VW+椰子乳;(3)1/2MS;(4)1/2MS+椰子乳;(5)N6;(6)N6+椰子乳;(7)N6+椰子乳+活性炭2g·L-1。继代增殖和分化成苗培养基:(8)N6+椰子乳+6-BA1.0mg·L-1(单位下同)+NAA0.2;(9)N6+椰子乳+6-BA0.2+NAA0.1;(10)N6+椰子乳+6-BA0.2+NAA0.1+活性炭2g·L-1;(11)花宝1号(美国Haponex公司产品,N∶P∶K=7∶6∶19)1.5g·L-1+花宝2号(美国Haponex公司产品,N∶P∶K=20∶20∶20)1.5g·L-1+椰子乳+6-BA0.2+NAA0.1。生根壮苗培养基:(12)N6+NA…     

Entwicklungsgeschichte und Ultrastruktur von Pollenkitt und Exine bei nahe verwandten entomophilen und anemophilen Angiospermensippen derAlismataceae,Liliaceae, Juncaceae,Cyperaceae, Poaceae undAraceae

Some closely related members of the monocotyledonous familiesAlismataceae, Liliaceae, Juncaceae, Cyperaceae, Poaceae andAraceae with variable modes of pollination (insect- and wind-pollination) were studied in relation to the ultrastructure of pollenkitt and exine (amount, consistency and distribution of pollenkitt on the surface of pollen grains). The character syndromes of pollen cementing in entomophilous, anemophilous and intermediate (ambophilous or amphiphilous) monocotyledons are the same in principal as in dicotyledons. Comparing present with former results one can summarize: 1) The pollenkitt is always produced in the same manner by the anther tapetum in all angiosperm sub-classes. 2) The variable stickiness of entomophilous and anemophilous pollen always depends on the particular distribution and consistency of the pollenkitt, but not its amount on the pollen surface. 3) The mostly dry and powdery pollen of anemophilous plants always contains a variable amount of inactive pollenkitt in its exine cavities. 4) A step-by step change of the pollen cementing syndrome can be observed from entomophily towards anemophily. 5) From the omnipresence of pollenkitt in all wind-pollinated angiosperms studied one can conclude that the ancestors of anemophilous angiosperms probably have been zoophilous (i.e. entomophilous) throughout.

     

Identification and Characterization of the First Equine Parainfluenza Virus 5

正Dear Editor,Parainfluenza virus 5 (PIV5), known as canine parainfluenza virus in the veterinary field, is a negative-sense,nonsegmented, single-stranded RNA virus belonging to the Paramyxoviridae family (Chen 2018). The virus was first reported in primary monkey kidney cells in 1954 (Hsiung1972), then it has been frequently discovered in various     

Pathogenic Characterization and Full Length Genome Sequence of a Reassortant Infectious Bursal Disease Virus Newly Isolated in Pakistan

<正>Dear Editor,Infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a nonenveloped virus belonging to the Birnaviridae family with a genome consisting of two segments of double-stranded RNA (segments A and B), targets B lymphocytes of bursa of Fabricious leading to immunosuppression. In Pakistan,poultry farming is the second biggest industry and IBD is the second biggest disease threating the poultry sector.However, there is limited genome information of IBDV     

Mink Circovirus Can Infect Minks,Foxes and Raccoon Dogs

正Dear Editor,Mink circovirus (MiCV), which is clustered in the genus Circovirus of the family Circoviridae, was first described in minks from farms in Dalian, China in 2013 (Lian et al.2014). The complete single-stranded circular genome of the virus is 1,753 nucleotides long and contains two major open reading frames (ORFs), designated ORF1 (Rep gene)and ORF2 (Cap gene)(Lian et al. 2014; Ge et al. 2018).Sequence analysis has shown that MiCV is most closely     

CypA Regulates AIP4-Mediated M1 Ubiquitination of Influenza A Virus

Cyclophilin A (CypA) is a peptidyl-prolyl cis/trans isomerase that interacts with the matrix protein (M1) of influenza A virus (IAV) and restricts virus replication by regulating the ubiquitin–proteasome-mediated degradation of M1. However,the mechanism by which CypA regulates M1 ubiquitination remains unknown. In this study, we reported that E3 ubiquitin ligase AIP4 promoted K48-linked ubiquitination of M1 at K102 and K104, and accelerated ubiquitin–proteasome-mediated degradation of M1. The recombinant IAV with mutant M1 (K102 R/K104 R) could not be rescued, suggesting that the ubiquitination of M1 at K102/K104 was essential for IAV replication. Furthermore, CypA inhibited AIP4-mediated M1 ubiquitination by impairing the interaction between AIP4 and M1. More importantly, both the mutations of M1 (K102 R/K104 R) and CypA inhibited the nuclear export of M1, indicating that CypA regulates the cellular localization of M1 via inhibition of AIP4-mediated M1 ubiquitination at K102 and K104, which results in the reduced replication of IAV.Collectively, our findings reveal a novel ubiquitination-based mechanism by which CypA regulates the replication of IAV.