山葵未成熟胚离体培养和植株再生

1　植物名称　山葵 (Ｅｕｔｒｅｍａｗａｓａｂｉ)日本品种岛根 3号。2　材料类别　开花 2 0ｄ后的荚果中剥出的未成熟种子 (种皮 未成熟胚 )。3　培养条件　基本培养基为自行设计的“贵山”(代号ＧＳ)配方[1 ] 。 ( 1 )诱导愈伤组织培养基为 :ＧＳ 6 ＢＡ 0 .1ｍｇ·Ｌ- 1 (单位下同 ) 2 ,4 Ｄ 1 ;( 2 )芽分化培养基为 :ＧＳ 6 ＢＡ 0 .5 ＺＴ 0 .5 ＩＢＡ0 .0 1 ;( 3)芽增殖培养基为 :ＧＳ 6 ＢＡ 0 .4;( 4 )生根培养基为 :ＧＳ ＩＢＡ 0 .1 ＮＡＡ 0 .1。以上培养基均含 3%蔗糖 [( 1 )含 2 % ]和 0 .6%琼脂粉 ,ｐＨ5 .8～ 5…     

西藏地区银白杨的组织培养和快速繁殖

1　植物名称　银白杨 (Ｐｏｐｕｌｕｓａｌｂａ)。2　材料类别　优良单株休眠枝水插萌动芽。3　培养条件　芽萌动培养基 :( 1 )ＭＳ ＫＴ 1 .0ｍｇ·Ｌ- 1 (单位下同 ) ＩＢＡ 0 .2 ;( 2 )ＭＳ ＫＴ 0 .5 ＩＢＡ 0 .1。诱导丛生芽培养基 :( 3)ＭＳ 6 ＢＡ0 .3 ＮＡＡ 0 .     

白花假龙胆的组织培养

1　植物名称　白花假龙胆 (Ｇｅｎｔｉａｎｅｌｌａａｌｂｉｆ ｐｒａ)。2　材料类别　野生品种白花假龙胆的胚轴、幼叶及未成熟种子 (采自青海省海北州 )。3　培养条件　 (1 )诱导培养基 :选用ＭＳ、Ｂ5和Ｎ6三种培养基。培养基以ＭＳ为基本培养基 ,分别附加不同激素浓度组合 :① 2 .4 Ｄ 3 .0ｍｇ·Ｌ- 1(单位下同 ) ;② 2 .4 Ｄ 1 .0 +ＫＴ 2 .0 ;③ 2 .4 Ｄ 2 .0+ＫＴ 1 .0 ;④ 2 .4 Ｄ 3 .0 +ＫＴ 1 .0 ;⑤ＮＡＡ 0 .5 +6 ＢＡ 0 .2。 (2 )继代培养基 :ＭＳ +2 .4 Ｄ 2 .0ｍｇ·Ｌ- 1+ＫＴ 0 .8ｍｇ·Ｌ- 1 。 (3 )分化培养基 :ＭＳ +…     

条纹十二卷的组织培养与快速繁殖

1　植物名称　条纹十二卷 (Ｈａｗｏｒｔｈｉａｆａｓｃｉａｔａ) ,大型和小型植株各 1种。2　材料类别　花葶。3　培养条件　启动脱分化培养基 :( 1 )改良ＭＳ ＫＴ 2ｍｇ·Ｌ- 1 (单位下同 ) ＮＡＡ 1 ＩＢＡ 0 .5 2 0 %椰子液体胚乳 ;不定芽诱导及增殖培养基 :( 2 )改良ＭＳ 6 ＢＡ 1 ＫＴ 2 ＩＢＡ 0 .4 2 0 %椰乳 ;长期继代增殖培养基 :( 3)改良ＭＳ 6 ＢＡ 1 ＮＡＡ0 1或 ( 4 )改良ＭＳ ＫＴ 2 ＩＢＡ 0 2 ,省去椰乳 ;诱导生根培养基 :( 5) 1 /2ＭＳ(大量元素减半 ,微量元素免用 ) ＩＢＡ 0 3。上述培养基均加 3%白…     

甜瓜的组织培养与植株再生

1　植物名称　甜瓜 (Ｃｕｃｕｍｉｓｍｅｌｏ)品种“伽师瓜”。2　材料类别　成熟甜瓜种子无菌苗的子叶。3　培养条件　种子萌发培养基 :(1 ) 1 / 2ＭＳ(大量元素减半 ) 2 %蔗糖。愈伤组织诱导培养基 :(2 )Ｍｉｌｌｅｒ 6 ＢＡ 5 .0ｍｇ·Ｌ- 1 (单位下同 ) ＺＴ 0 .0 1 3 %蔗糖。丛生芽诱导培养基 :(3 )Ｍｉｌｌｅｒ 6 ＢＡ 2 .0 3 %蔗糖 ;(4)Ｍｉｌｌｅｒ 6 ＢＡ 0 .5 3 %蔗糖。芽伸长培养基 :(5 )Ｍｉｌｌｅｒ 6 ＢＡ 0 .1 3 %蔗糖 ;(6 )Ｍｉｌｌｅｒ 6 ＢＡ 0 .0 1 3 %蔗糖。生根培养基 :(7) 1 /2ＭＳ(大 ) ＮＡＡ 0 .0…     

玉叶金花的组织培养和植株再生

1　植物名称　玉叶金花 (Ｍｕｓｓａｅｎｄａｐｕｂｅｓｃｅｎｓ)。2　材料类别　嫩茎、幼叶。3　培养条件　以ＭＳ为基本培养基。诱导愈伤组织附加 :( 1 )ＮＡＡ 0 .2ｍｇ·Ｌ- 1 (单位下同 ) ＫＴ0 .2 5 6 ＢＡ 0 .1 ;( 2 )ＮＡＡ 0 .5 ＫＴ 0 .2 5 6 ＢＡ0 .1 ;( 3)ＮＡＡ 0 .5 6 ＢＡ 0 .1 ;( 4 ) 2 ,4 Ｄ 0 .2 ＫＴ0 .1。继代培养基附加 :( 5 ) 6 ＢＡ 4 ＫＴ0 .2 5 ＩＡＡ 0 .5。不定芽诱导及增殖培养基附加 :( 6)6 ＢＡ 4 ＫＴ 0 .5 ＩＡＡ 0 .1。生根培养基 :( 7)1 /2ＭＳ ＩＢＡ 0 .5。上述培养基均含 0 .7%琼脂、3%蔗…     

植物组织培养简报摘编

植物材料、外植体 培养基 (ｍｇ·Ｌ- 1 ) 结　　果作者 (单位 )普通甜菜(Ｂｅｔａｖｕｌｇａｒｉｓ)无菌苗顶芽种子萌发培养基 :( 1)ＭＳ 6 ＢＡ 0 .5 ＴＩＢＡ0 .1 ＩＡＡ 0 .2 ;芽增殖培养基 :( 2 )ＭＳ 6 ＢＡ 0 .5 ＴＩＢＡ 0 .2 ;壮苗培养基 :( 3)ＭＳ 6 ＢＡ 0 .2     

大豆茎尖离体培养再生植株

1　植物名称　大豆 (Ｇｌｙｃｉｎｅｍａｘ)品种中黄 4号、早熟 1 8和中品 661。2　材料类别　茎尖。3　培养条件　生芽培养基 :( 1 )ＭＳ 6 ＢＡ 3ｍｇ·Ｌ- 1 (单位下同 )。不定芽伸长培养基 :( 2 ) 1 /2ＭＳ 6 ＢＡ 0 .1 ;( 3)ＭＳ ＧＡ30 .5。生根培养基 :( 4 ) 1 /2ＭＳ ＮＡＡ 0 .1。以上培养基均含蔗糖 3%、琼脂0 .8% ,ｐＨ 5 .8;培养室温度 ( 2 6± 2 )℃ ;光照时间每天 1 6ｈ ,光照度 1 60 0～ 2 0 0 0ｌｘ。4　生长与分化情况4.1　靶组织区的制备　取大豆种子 ,以 70 %酒精处理 1ｍｉｎ ,1 %次氯酸钠消毒 1 5ｍｉｎ后 ,用无…     

山葵的离体快速繁殖

1　植物名称　山葵 (Ｅｕｔｒｅｍａｗａｓａｂｉ)日本品种岛根 3号。2　材料类别　无菌萌发的种子苗。3　培养条件　基本培养基为自行设计的“贵山”(代号ＧＳ)配方 [其大量元素组分为 :每Ｌ含(ＮＨ4) 2 ＳＯ40 .32 1 ｇ、ＫＮＯ31 .2 81 ｇ、ＫＨ2 ＰＯ40 .2 0 0ｇ、ＣａＣｌ2 · 2Ｈ2 Ｏ 0 .887ｇ和ＭｇＳＯ4· 7Ｈ2 Ｏ0 .746ｇ ,铁盐、微量元素和维生素组分均与ＭＳ配方相同 ]。种子萌发培养基为 :( 1 )ＧＳ 6 ＢＡ 0 .2ｍｇ·Ｌ- 1 (单位下同 ) ;芽增殖培养基为 :( 2 )ＧＳ 6 ＢＡ 0 .4;生根培养基为 :( 3)ＧＳ ＩＢＡ 0 .4。蔗糖…     

星花绣线菊的组织培养及快速繁殖

1　植物名称　星花绣线菊 (Ｓｐｉｒａｅａｊａｐｏｎｉｃａｖａｒ.ｓｔｅｌｌｅｒｉｓ)。2　材料类别　茎尖、茎段、叶片、叶柄。3　培养条件　 ( 1 )愈伤组织诱导培养基 :ＭＳ 2 ,4 Ｄ 2 .0ｍｇ·Ｌ- 1 (单位下同 ) ＫＴ 0 .3;( 2 )愈伤组织继代培养基 :6,7 Ｖ 2 ,4 Ｄ 2 .0 ＫＴ 0 .2 5 ＮＡＡ 1 .0 ＬＨ 2 0 0 0 ;( 3)芽诱导培养基 :ＭＳ 6 ＢＡ 2 .0 ＮＡＡ 0 .1 ;( 4 )芽增殖培养基 :ＭＳ 6 ＢＡ0 .2 5 ＮＡＡ 0 .1 ;( 5 )生根培养基 :1 /2ＭＳ 6 ＢＡ0 .2 5 ＮＡＡ 0 .5。上述培养基的蔗糖含量为 3% ,琼脂为 0 .7% ,…     

香蕉草的固体培养和液体培养

1　植物名称　香蕉草 (Nymphoidesaquatica)。2　材料类别　嫩叶片 ( youngleaves)。3　培养条件　丛生芽诱导和增殖固体培养基 :( 1 )MS+ 6 BA 2 .0mg·L- 1 (单位下同 ) +NAA 0 .2 ;( 2 )MS + 6 BA 0 .5～ 3.0 +NAA 0 .2 ;( 3)MS + 6 BA0 .5～ 3.0 +IAA 0 .3;( 4 )MS + 6 BA 0 .5～ 3.0 +IBA 0 .3。丛生芽诱导和增殖液体培养基 :( 5 ) 1 /2MS + 6 BA 1 .0 +IAA 0 .3。生根培养基 :( 6)MS +IBA 0 .3+NAA 0 .2。以上培养基均附加 2 %白糖(sugar) ,0 .75 %的琼脂 (agar,液体培养基除外 ) ,pH5 .8,培养温度为 ( 2 5± 2 )…     

培养基和培养条件与红豆杉细胞培养中褐化的关系

就近几年来通过培养基和培养条件的选择来控制和防止红豆杉细胞培养中的褐化问题作了介绍.     

Culture and Rights after Culture and Rights

Building on a critical, theoretical approach outlined in Culture and Rights: Anthropological Perspectives (Cowan et al. 2001a), I posit rights processes as complex and contradictory: Both enabling and constraining, they produce new subjectivities and social relations and entail unintended consequences. To encourage interdisciplinary engagement on these themes, I explore selected texts that consider the relationship between culture and rights, addressing two literatures: (1) debates on culture, rights, and recognition in the context of multiculturalism among political philosophers and (2) an emerging literature by anthropologists, feminists, critical legal scholars, and engaged practitioners analyzing empirical cases. Although political philosophers elucidate ethical implications and clarify political projects, an outmoded arsenal of theoretical concepts of "culture,""society," and "the individual" has hampered their debates. When accounts are both theoretically informed and empirically grounded, contradictions, ambiguities, and impasses of culture and rights are more fully explored and the liberal model of rights and multiculturalism is more open to interrogation.     

红枫增殖生长培养基配方研究

目的:研究红枫组织培养中增殖阶段的培养基,以期筛选出最适增殖培养基配方,为最终建立红枫无毒快繁体系奠定基础。方法:以MS培养基为基本培养基,附加不同浓度的CPPU和6-BA,并以食用白砂糖代替分析纯蔗糖,对红枫进行增殖培养,比较生长状况。结果:CPPU和6-BA均能促进红枫单芽茎段的腋芽增殖,其中以CPPU的效果较好。结论:最适增殖培养基为(MS,30g/L蔗糖,6.5g/L琼脂,CPPU0.3mg/L,IBA0.05mg/L),可以用食用白砂糖代替分析纯蔗糖对腋芽进行增殖培养。     

Evaluation of Four Blood Culture Systems Using Parallel Culture Methods

Commercially prepared Vacutainer thioglycolate broth with SPS (TBSPS), the B-D Vacutainer culture tube with SPS (VCT), the Pfizer E-Vac hypertonic brucella broth with SPS (BBSPS), and our own Trypticase-soy hypertonic broth with SPS (TSBH) were studied by parallel culture methods for their ability to allow the survival of a wide spectrum of organisms, their ability to allow recovery of organisms, and the time required to obtain a recovery from clinical blood cultures. Organisms considered to be clinically significant were recovered from 176 blood cultures. The TSBH system produced all 176 recoveries, the BBSPS system allowed for 170 recoveries, the TBSPS recovered 131, and the VCT accounted for 106 recoveries. The time required to recover any given organism from both the BBSPS and the TSBH systems did not exceed 48 h, whereas 86 of the 131 recoveries from the TBSPS system required more than 72 h and 74 of the 106 VCT recoveries also required more than 72 h. A wide spectrum of organisms were recovered from both the BBSPS and the TSBH systems. The TBSPS system failed to allow the recovery of many streptococci and Pseudomonas spp. and the VCT system failed to recover many organisms.