颈部低度迷走神经刺激对老年房颤患者血清CRP、TNF-α及MMP-9/TIMP-1水平的影响

目的:探讨颈部低度迷走神经刺激对老年房颤患者血清C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)及基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶抑制剂-1(TIMP-1)的影响。方法:选取我院收治的老年房颤患者126例,根据治疗方法的不同分为两组。对照组63例,予常规治疗,日1次,7天为1个疗程,治疗2个疗程;实验组63例,在对照组的基础上加以颈部低度迷走神经刺激术。治疗后,观察并比较两组患者的CRP、TNF-α及MMP-9、TIMP-1的变化情况。结果:治疗后,两组患者的血清CRP、TNF-α、MMP-9及TIMP-1均较治疗前显著降低,且实验组显著低于对照组,差异均有统计学意义(P0.05)。实验组治疗后6个月内房颤的发生率显著降低,差异均有统计学意义(P0.05)。结论:颈部低度迷走神经刺激能够有效降低老年房颤患者的复发率,这可能与其降低血清CRP、TNF-α,MMP-9、TIMP-1的水平有关。     

地高辛对老年慢性心衰患者血清CA125，BNP及MMP-9 水平的影响

目的:探讨地高辛片对老年慢性心力衰竭患者血清血清糖类抗原(CA125)、心钠肽(ANP)、脑钠肽(BNP)、基质金属蛋白酶9(MMP-9)及金属蛋白酶组织抑制剂(TIMP-1)水平的影响。方法:收集我院老年慢性心力衰竭患者100例,随机分为对照组和实验组,每组各50例,对照组患者给予常规治疗,实验组患者在对照组的基础上给予地高辛片。比较治疗前后两组患者血清糖类抗原(CA125)、心钠肽(ANP)、脑钠肽(BNP)、基质金属蛋白酶9(MMP-9)及金属蛋白酶组织抑制剂(TIMP-1)水平。结果:与治疗前相比,两组患者血清CA125、ANP、BNP及MMP-9水平降低,TIMP-1水平升高(P0.05);治疗后与对照组相比,实验组CA125水平(34.05±4.63)较低,ANP水平较低(216.98±27.65),BNP水平较低(437.62±45.26),MMP-9水平较低(550.17±65.15),TIMP-1水平较高(182.32±21.78)(P0.05)。结论:地高辛片能够改善老年慢性心力衰竭患者心肌功能,推测其机制可能与降低血清CA125、ANP、BNP、MMP-9水平,升高TIMP-1水平有关。     

血栓通联合依达拉奉对老年急性脑梗死患者氧化低密度脂蛋白和MMPs水平的影响及临床疗效

目的:探讨血栓通联合依达拉奉对老年急性脑梗死患者氧化低密度脂蛋白及MMPs水平的影响及临床疗效。方法:收集我院收治的126例急性脑梗死患者,随机分为实验组和对照组,每组各63例。对照组患者给予血栓通注射液治疗;实验组患者在对照组基础上给予依达拉奉治疗。观察并比较两组患者治疗前后血清氧化低密度脂蛋白(Ox-LDL)、基质金属蛋白酶2(MMP-2)及基质金属蛋白酶9(MMP-9)水平的变化情况以及临床疗效。结果:与治疗前相比,治疗后两组患者Ox-LDL,MMP-2及MMP-9水平均下降,差异具有统计学意义(P0.05);与对照组相比,实验组患者Ox-LDL,MMP-2及MMP-9水平较低,差异具有统计学意义(P0.05);与对照组相比,实验组患者的临床疗效较高,差异具有统计学意义(P0.05)。结论:血栓通联合依达拉奉能够降低老年急性脑梗死患者氧化低密度脂蛋白(Ox-LDL)、基质金属蛋白酶2(MMP-2)以及基质金属蛋白酶9(MMP-9)水平,临床疗效较好。     

醒脑静注射液对老年肺炎支原体肺炎患者血清TNF-alpha、CRP水平及免疫 功能的影响

目的：探讨醒脑静注射液对老年肺炎支原体肺炎患者血清肿瘤坏死因子-alpha(TNF-alpha)、C- 反应蛋白(CRP)水平及免疫功能影 响。方法：收集我院收治的老年肺炎支原体肺炎患者80例,随机分为实验组和对照组。对照组予阿奇霉素治疗,实验组在对照组 基础上加用醒脑静注射液。分别于治疗前、治疗后测定和比较两组血清TNF-alpha、CRP、CD3+、CD4+、CD8+水平,并对比较两组患者 咳嗽、头痛、发热等症状及X 线检查肺部阴影消失时间。结果：①治疗后,两组患者血清TNF-alpha、CRP水平均下降,实验组比对照组 下降更明显;CD3+、CD4+均上升,CD8+均下降,实验组较对照组上升及下降均更明显,差异有统计学意义(P＜0.05);②治疗后,实 验组咳嗽、头痛、X 线肺部阴影等各症状及体征消失时间均明显短于对照组,差异有统计学意义(P＜0.05)。结论：醒脑静注射液可 显著降低老年肺炎支原体肺炎患者血清TNF-alpha、CRP水平,调节机体细胞免疫功能,显著改善患者的症状及体征。     

经皮椎体成形术对老年骨质疏松性胸腰椎压缩骨折患者血清MMP-3、TIMP-1、IL-6及疗效的影响

目的:探讨经皮椎体成形术对老年骨质疏松性胸腰椎压缩骨折患者血清基质金属蛋白酶-3(MMP-3)、基质金属蛋白酶抑制因子-1(TIMP-1)、白细胞介素-6(IL-6)及疗效的影响。方法:选择60例2016年10月到2017年3月我院接诊的老年骨质疏松性胸腰椎压缩骨折患者,依照随机数表法分为实验组和对照组,每组30例,在都给予常规药物治疗的基础上,对照组给予复位枕垫和康复训练治疗,实验组采用经皮椎体成形术治疗。结果:治疗前,两组血清MMP-3、TIMP-1、IL-6水平无差异(P0.05);治疗后,两组血清MMP-3、IL-6水平均降低,实验组下降幅度更大,两组血清TIMP-1水平均升高,实验组上升幅度更大(P0.05);治疗前,两组患者VAS评分无差异(P0.05),治疗1周及治疗后,实验组患者VAS评分均低于对照组(P0.05);治疗前,两组患者Cobb角无差异(P0.05),治疗后,两组患者Cobb均明显下降,实验组较对照组下降更大(P0.05);治疗前,两组患者伤椎高度比无差异(P0.05),治疗后,两组伤椎高度比明显下降,实验组低于对照组(P0.05);治疗后,实验组有效率96.67%大于对照组的76.67%,差异显著(P0.05)。结论:经皮椎体成形术能有效降低患者血清MMP-3、IL-6水平,恢复TIMP-1水平,能显著提高治疗老年骨质疏松性胸腰椎压缩骨折的疗效     

房颤不同时期结构重构的血清学变化

目的:探讨转化生长因子-β1(TGF-β1)、基质金属蛋白酶-9(MMP-9)、金属蛋白酶组织抑制因子-1(TIMP-1)、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、白介素-10(IL-10)与阵发性或持续性房颤结构重构的关系。方法:入选患者分为3组,其中持续性房颤组30例、阵发性房颤组45例,以及32例阵发性室上速(包括预激综合征)作为对照组,通过ELISA方法检测上述患者左房血清的TGF-β1、MMP-9、TIMP-1、TNF-α、IL-6、IL-10水平,超声测量左心房长径。分析上述细胞因子水平与阵发性房颤或持续性房颤的关系。结果:阵发性房颤组与对照组相比,左房内径增大、TGF-β1、TNF-α升高(P0.05),IL-10、TIMP-1降低(P0.05),MMP-9无统计学差异。持续性房颤组与对照组相比,TGF-β1无统计学差异(P0.05),MMP-9、TNF-α、IL-6均升高(P0.05),IL-10、TIMP-1降低(P0.05);阵发性房颤组TGF-β1高于持续性房颤组(P0.05),持续性房颤组左房内径及MMP-9高于阵发性房颤组(P0.05)。结论:阵发性房颤患者左房扩大,已出现结构重构,结构重构的血清学变化以TGF-β1升高为主;持续性房颤患者结构重构血清学变化以MMP-9、IL-6升高为主。     

肺结核并发纤维化患者BALF和血清MMP及TIMP-1检测的临床意义

目的:探讨肺结核并发纤维化患者支气管肺泡灌洗液(BALF)及血清中基质金属蛋白9(MMP-9)及基质金属蛋白酶组织抑制剂l(TIMP-1)含量与肺纤维化及肺功能的关系.方法:酶联免疫(ELISA)法检测我院2009年3月至2009年9月68例肺结核并发纤维化患者(纤维化组)及30例无肺部疾病患者(对照组)的支气管肺泡灌洗液及血清中MMP-9及TIMP-1的含量,同时进行肺功能检查.结果:纤维化组患者的BALF及血清MMP-9的含量与对照组怠者相比无统计学意义,但纤维化组患者的BALF及血清TIMP-1的含量显著高于对照组(P＜0.05),且纤雏化组患者的BALF及血清MMP-9/TIMP-1比值低于对照组(P＜0.05).患者的动脉血氧分压、肺活量等肺功能指标与TIMP-1呈正相关,而与MMP-9/TIMP-1比值呈负相关(P＜0.05).结论:肺结核患者肺纤维化的发生可能与TIMP-1水平升高及MMP-9/TIMP-1比值降低相关,降低TIMP-9的水平可能是防止肺结核惠者并发肺纤维化的一种新途径.     

瓣膜手术同期射频消融改良迷宫术治疗心脏瓣膜病并发房颤患者的疗效及对血清细胞因子的影响

目的:分析瓣膜手术同期射频消融改良迷宫术治疗心脏瓣膜病并发房颤患者的疗效及对血清细胞因子的影响。方法:将80例心脏瓣膜病并发房颤患者依据简单随机法分为对照组和观察组,每组40例。对照组采用心脏瓣膜置换术治疗,观察组采用心脏瓣膜置换术同期射频消融改良迷宫术治疗,比较两组窦性心律转复情况,手术情况,手术前后心功能、血清金属蛋白酶组织抑制因子-1(TIMP-1)、基质金属蛋白酶-1(MMP-1)和基质金属蛋白酶-9(MMP-9)水平的变化以及术后并发症的发生情况。结果:观察组术后当天、术后1月、术后3月及术后6月的窦性心律转复率均显著高于对照组(P0.05),体外循环时间、主动脉阻断时间及术后24 h引流量均明显多于对照组(P0.05)。两组呼吸机使用时间和监护室时间比较差异无统计学意义(P0.05)。术后6个月,两组左室舒张末期内径、左室收缩末期内径、血清MMP-1和MMP-9水平均较术前显著下降,且观察组以上指标明显低于对照组;两组LVEF及血清TIMP-1水平较术前显著上升,且观察组以上指标均显著高于对照组(P0.05)。两组术后均无严重并发症发生。结论:瓣膜手术同期射频消融改良迷宫术治疗心脏瓣膜病并发房颤安全有效,早期窦性心律的转复率高,且可改善患者血清TIMP-1、MMP-1、MMP-9水平。     

肺癌患者血清中VEGF,TIMP-1和MMP-9水平变化及临床意义

目的:研究肺癌患者血清中血管内皮生长因子(VEGF)、组织金属蛋白酶抑制剂1(TIMP-1)、基质金属蛋白酶9(MMP-9)水平变化及临床意义。方法:选取2014年3月至2016年3月来我院治疗的91例肺癌患者为病例组,同期选取40例健康者为对照组,酶联免疫吸附测定法(ELISA)测定两组血清VEGF、TIMP-1、MMP-9水平,分析肺癌患者上述指标与病理特征的关系,并采用spearman检验分析相关性。结果:病例组血清VEGF、TIMP-1、MMP-9水平均高于对照组,差异均具有统计学意义(P0.05)。肺癌患者血清VEGF、TIMP-1、MMP-9水平均与肿瘤体积大小、TNM分期、淋巴结转移、远处转移有关(P0.05)。肺癌患者血清MMP-9与TIMP-1正相关(r=0.337,P0.05)、血清MMP-9与VEGF正相关(r=0.312,P0.05)、血清TIMP-1与VEGF正相关(r=0.316,P0.05)。结论:血清VEGF、TIMP-1、MMP-9相互作用、协同参与肺癌的发生及侵袭转移,可作为肺癌诊断及预后评估的生物学标志物。     

COPD大鼠模型气道MMP-9和TIMP-1表达的研究

目的:探讨基质金属蛋白酶-9(MMP-9)及组织金属蛋白酶抑制因子-1(TIMP-1)在慢性阻塞性肺疾病(COPD)大鼠模型气道的表达.方法:Wistar大鼠25只随机分为COPD模型组和正常对照组,采用单纯被动吸烟法建立COPD大鼠模型,以酶联免疫吸附法(ELISA)测定两组大鼠血清及支气管肺泡灌洗液(BALF)中MMP-9和TIMP-1的表达水平.结果:COPD组血清及BALF中MMP-9和TIMP-1的表达明显高于正常组(P＜0.05和P＜0.01);COPD组血清及BALF的MMP-9/TIMP-1低于正常组血清及BALF的MMP-9/TIMP-1,差异有统计学意义(P＜0.05);COPD组和正常组BALF中MMP-9和TIMP-1水平高于血清中MMP-9和TIMP-1水平,但差异无统计学意叉(P＞0.05).结论:MMP-9和TIMP-1平衡失调参与了COPD的发病,调节MMP-9和TIMP-1的平衡可能是治疗COPD的新方法.     

六种藓类植物茎的比较解剖学研究

通过对6种藓类植物,即褶叶青藓(Brachythecium salebrosum(Web.et Mohr.)B.S.G.)、湿地匐灯藓(Plagiomnium acutum(Lindb.)Kop.)、侧枝匐灯藓(Plagiomnium maximoviczii(Lindb.)Kop.)、大凤尾藓(Fissidensnobilis Griff.)、大羽藓(Thuidium cymbifolium(Doz.et Molk.)B.S.G.)和大灰藓(Hypnum plumaeforme Wils.)嫩茎和老茎的石蜡切片和显微观察发现,同一藓类植株的嫩茎和老茎,茎结构稳定,不同种藓类植物茎横切面具有不同特征.植物体茎横切面形状、表层细胞的层数、细胞大小和细胞壁厚薄、皮层细胞大小和形状、中轴的有无以及比例等特征可以作为藓类植物的分科分类依据之一.     

Cause of Senescence of Nine Sorts of Flowers

The levels of endogenous phytohormones and respiratory rate in nine sorts of flowers such as Cymbidium faberi Rolfe, Nopalxochia ackermannii Kunth and others were investigated both at full bloom and senescence and meanwhile the effect of exogenous phytohormones on prolonging the blossoms and promoting ethylene production were tested. There is a high content of endogenous ethylene in all the long-lived flowere, about 3–16 folds higer than the short-lived ones. There is a high level of ABA at full blooming flowers of short-lived flowers, in which there is no or only some cytokinins in it, but the ratio of CTK (6BA+zeatin)/ABA is smaller(l.7). The endogenous ABA reached a much higher level at senescence in all nine sorts of flowers, so it is reasonable to consider that it is ABA which plays an important role of regulation in controlling flower's senescence. There is a much higher level of GA3 and zeatin in the long-lived flowers which is not demonstrated in the shortlived ones. The respiratory rate is one of the factors controtling the longevity of flowers, but it does not play a decided role. Application of 6BA and zeatin prolongs distinctly orchid’s longevity, however exogenous IAA through the promotive action on ethylene production, evidently extends the longevity of the flowers of the Nopalxochia ackermannii Kunth.     

龙胆科药用植物化学成分的研究现状

龙胆科植物在我国的分布范围很广,且多数为药用植物,其多数种属的药用植物,至今其化学成分尚未被系统研究。综述了目前龙胆科药用植物的化学成分的研究现状及一般提取方法,对近年来发现的环烯醚萜及裂环烯醚萜类化合物进行了总结,为本科药用植物的更深入研究提供了参考。     

The effects of glutamine of the maintenance of embryogenic cultures of Cryptomeria japonica

Summary Embryogenic tissues of sugi (Cryptomeria japonica) were induced on a modified Campbell and Durzan (CD) medium containing 1 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 600 mg l⁻¹ glutamine, and subcultured in the medium of the same composition for over 1 yr. This resulted in a mixed culture of embryogenic and non-embryogenic cells. When embryogenic cells were isolated and cultured independently, their capacity to form embryogenic aggregates was lost. Thus, the non-embryogenic cells present within a mixed culture system were essential to the formation of embryogenic aggregates. When embryogenic tissues were isolated and cultured independently on a high glutamine-containing (2400 mg l⁻¹) medium, dry weights and endogenous levels of glutamine increased, and the tissue could generate a large number of embryogenic aggregates. Amino acid analysis of embryogenic and non-embryogenic cells from the maintenance culture indicated a higher level of glutamine was present in the latter. The high endogenous level of glutamine in the non-embryogenic portion of mixed cell masses may be the supplier of glutamine for maintaining the embryogenic property of the tissues.     

Specificity of action of piperidylcholine derivatives as substrates of cholinesterases of various origin

The review deals with study of enzymologic properties of a novel highly specific acetylcholinesterase substrate, N-(β-acetoxyethyl) piperidinium iodomethylate (“piperidylcholine”), and its 30 derivatives that were tested as effectors of cholinesterases of mammals and various species of Pacific squids. It was proven for the first time that responsible for specificity of action was structure of cyclic ammonium grouping of the alcohol part of molecule of the ester substrate. Analysis of specificity is performed based on enzymatic hydrolysis parameters—activity of catalytic center of cholinesterases and bimolecular constant of the reaction rate that are determined at optimal and low substrate concentrations. Among the specially synthesized group of thioester compounds there is revealed one more highly specific acetylcholinesterase substrate—N-(β-acetoxyethyl) piperidinium.     

真菌类遗传学分析的知识结构教学

本文以认知结构理论为指导,讨论了真菌类遗传分析与高等动植物遗传分析的内在联系,认为利用这种内在联系进行教学可收到好的效果并说明了作者的具体教学过程。 Abstract:In the paper, the relationship between genetic analysis of Fungi and genetic analysis of high animal and plant was discussed.A good results were obtained when we adopted this method in the teaching.     

Photoregulation of seed germination of wild-type and of an aurea-mutant of tomato

Seed germination of an aurea mutant of tomato ( Lycopersicon esculentum Mill.) is promoted by continuous irradiation with red, far-red or long-wavelength far-red (758 nm) light as well as by cyclic irradiations (5 min red or 5 min far-red/25 min darkness). Far-red light applied immediately after each red does not change the germination behaviour. Seed germination of the isogenic wild-type, cv. UC-105, is promoted by continuous and cyclic red light while it is inhibited by continuous and cyclic far-red light and by continious 758 nm irradiation. Far-red irradiation reverses almost completely the promoting effect of red light. The promoting effect (in the aurea mutant) and the inhibitory effect (in the wild-type) of continuous far-red light do not show photon fluence rate dependency above 20 nmol m⁻² s⁻¹. It is concluded that phytochrome controls tomato seed germination throgh low energy responses in both the wild type and the au mutant. The promoting effect of continuous and cyclic far-red light in the au mutant can be attributed to a greater sensitivity to P_fr.     

The mechanism of hatching of eggs of Haemonchus contortus

Fluid collected from hatching eggs of Haemonchus contortus contained a lipase which hydrolysed 2-naphthyl laurate (about 0·7 μmol naphthol freed /h/10⁶ eggs). The fluid also hydrolysed l-leucinamide (about 2·3 μmol leucine freed/h/10⁶ eggs). The fluid when added to normal or heated eggs caused ‘hatching’. ‘Hatching’ also occurred in exsheathing fluid from infective juveniles and in a preparation of pancreatic lipase containing leucine aminopeptidase. A purified mammalian leucine aminopeptidase in combination with several different lipases did not attack egg shells.The ‘spontaneous’ hatching of eggs of H. contortus was strongly inhibited by 1,10-phenanthroline, 10^?3M, and this inhibition was reversed by Zn²⁺. However, the inhibition of ‘hatching’ of eggs in externally applied hatching fluid, or the hydrolysis of leucinamide in hatching fluid was generally less marked.