秀丽隐杆线虫体内脂滴的尼罗红染色观察

秀丽隐杆线虫(Caenorhabditis elegans)为常见的生物实验模式之一,也是最近作为研究脂肪沉积的一种较理想的模型,其肠道是脂肪沉积的主要场所。本文使用尼罗红染液对秀丽隐杆线虫体内的脂肪进行染色,在荧光显微镜下可见其肠道周围呈现强烈桔黄色,较好的显示了线虫体内的脂肪沉积部位,为后续研究线虫脂肪沉积的调控机制奠定了基础。     

秀丽隐杆线虫体内脂肪染色方法的探究

近几十年来,秀丽隐杆线虫(Caenorhabditis elegans)因其结构简单、通体透明、生命周期短和易于培养,常作为一种模式生物被广泛用于现代发育生物学、遗传学、抗衰老和及脂肪调控等方面的研究。本文探索了一种对秀丽隐杆线虫体内脂肪的油红O染色方法,利用1%Triton X-100的透过作用,线虫体内脂滴可被油红O更好的着色,镜下观察颜色鲜红,染色效果较好,为以后研究线虫脂肪调控奠定了基础。     

不同浓度亳菊水提物对秀丽隐杆线虫脂滴凝聚影响观察

目的探讨毫菊对秀丽隐杆线虫脂肪沉积的影响。方法用不同浓度毫菊水提取液配制NGM培养基,培养同步化秀丽隐杆线虫,成虫经油红0染色,异丙醇萃取,测定OD值,并与普通NGM培养基培养的同步化线虫相比较。结果随着浓度的提高,线虫体内的脂肪沉积量呈现出一定的曲线变化,其具体机制有待于进一步研究。     

铜对秀丽隐杆线虫毒性效应的研究

为了阐明铜(Cu)对秀丽隐杆线虫Caenorhabditis elegans长期作用的毒性效应,对实验室多代筛选的耐铜型秀丽隐杆线虫进行了寿命、衰老、发育、生殖和运动等生物学指标的研究.结果显示耐铜型秀丽隐杆线虫与野生型秀丽隐杆线虫相比其寿命缩短、衰老提前、个体发育受到抑制,且出现繁殖率降低、生殖能力减弱、运动行为存在障碍等一系列生理变化.本文为理解与阐明Cu的毒性效应提供了实验资料,有助于深入开展Cu毒性机理的研究.     

细菌与秀丽隐杆线虫之间互作关系的研究进展

秀丽隐杆线虫(Caenorhabditis elegans)以其个体小、易培养、生活周期短等优势成为生物发育、衰老、神经及免疫相关机制研究的模式生物.它在实验室培养时主要靠饲喂大肠杆菌OP50,有报道,细菌及其代谢物对线虫的代谢、行为和寿命有至关重要的影响.因此,作为一个遗传模型,秀丽隐杆线虫可以帮助研究微生物与宿主相...     

秀丽隐杆线虫先天免疫信号转导途径

秀丽隐杆线虫因其结构简单、易于培养、生命周期短等特点作为一种模式生物已广泛应用于神经系统、衰老机制及细胞程序性死亡的研究。与高等生物不同,秀丽隐杆线虫缺少适应性免疫途径,只有先天免疫途径在抗病原菌、抗氧化应激等方面发挥重要的作用。其体内的胰岛素/胰岛素样生长因子(insulin/ IGF-1)、转化生长因子β(transforming growth factor β,TGF-β)、丝裂原激活的蛋白激酶(mitogen activated protein kinases,MAPK)和细胞程序性死亡(programmed cell death,PCD)4条免疫相关信号转导途径在不同的环境发挥着主要作用。同时,秀丽隐杆线虫的先天免疫系统在进化中有许多保守之处,这为高等生物的免疫机制研究提供了新思路。据此,就有关秀丽隐杆线虫先天免疫信号转导途径的研究进展进行了简述,期望能为人类等高等生物相关联的免疫作用研究提供借鉴和参考。     

羊栖菜多糖抑脂沉积及其机制研究

目的研究羊栖菜多糖(SFPS)对秀丽隐杆线虫脂肪沉积的影响,并探讨其机制。方法用不同浓度SFPS配制NGM培养同步化秀丽隐杆线虫,经油红O染色后,裂解虫体,在490nm波长处测定吸光度值,同时采用外翻肠囊法,考察不同浓度SFPS被大鼠小肠吸收的情况。结果在含不同浓度SFPS配制的NGM培养基上生长的线虫,当SFPS浓度越高,线虫吸光度值越小,与对照组相比差异显著。在低剂量浓度下(0.6mg/ml),大鼠小肠对羊栖菜多糖吸收不明显。结论SFPS可减少动物体内脂质含量,其机制可能与小肠的吸收有关。     

阿尔茨海默病的秀丽隐杆线虫模型及其应用

秀丽隐杆线虫在遗传背景、研究手段上具有其独特的优势,以它为模型的研究集中于生命科学和医学研究的多个领域,阿尔茨海默病(AD)的研究也列于其中。虽然它神经系统结构简单,但它神经元的功能和神经递质都与其他动物类似,是研究神经退行性疾病机制的良好在体模型。本文就秀丽隐杆线虫在AD的研究进展进行了综述,主要包括AD相关基因在秀丽隐杆线虫中的保守性、AD相关基因转基因模型及其在研究治疗AD药物上的应用。     

秀丽隐杆线虫模型在抗衰老研究中的应用

随着人口老龄化问题的凸显,衰老相关的研究越来越被重视。秀丽隐杆线虫（Caenorhabditis elegans）是抗衰老研究领域中非常重要的生物模型,具有生命周期短、易于培养和观察等优点,但与其他哺乳动物模型相比仍有一些局限性,如DNA甲基化的缺乏等。本文主要综述了秀丽隐杆线虫模型在抗衰老研究和药物筛选中的应用,包括抗衰老药物对线虫寿命和抗性的测定与评估、药物筛选以及健康衰老研究中的应用,并概括了该模型的优势和局限性,为秀丽隐杆线虫模型在抗衰老研究中的应用提供理论依据。     

多吃并不一定会长胖

美国加州大学旧金山分校的Kaveh Ashrafi等人以秀丽隐杆线虫（Caenorllabditis elegans）为实验材料,利用RNA干扰技术研究了250多种基因,以分析神经递质serotonin对进食和脂肪的影响机制。     

秀丽隐杆线虫脂肪染色的方法比较(英文)

肥胖及其相关的疾病影响了越来越多的人.在哺乳动物中调节脂肪代谢的因子和脂肪代谢途径在线虫中也是保守存在的,因此线虫是脂肪研究的良好动物模型.在研究线虫脂肪代谢中,存在多种脂肪染色方法,而不同方法所表征的脂肪含量存在一定的差异,通过各种染色方法检测基因突变或者饥饿引起的脂肪含量变化.实验结果表明,采用油红、苏丹黑、尼罗红染料的固定染色法均能够正确地表征线虫的贮存脂肪.     

Stressed-induced TMEM135 protein is part of a conserved genetic network involved in fat storage and longevity regulation in Caenorhabditis elegans

Disorders of mitochondrial fat metabolism lead to sudden death in infants and children. Although survival is possible, the underlying molecular mechanisms which enable this outcome have not yet been clearly identified. Here we describe a conserved genetic network linking disorders of mitochondrial fat metabolism in mice to mechanisms of fat storage and survival in Caenorhabditis elegans (C. elegans). We have previously documented a mouse model of mitochondrial very-long chain acyl-CoA dehydrogenase (VLCAD) deficiency. We originally reported that the mice survived birth, but, upon exposure to cold and fasting stresses, these mice developed cardiac dysfunction, which greatly reduced survival. We used cDNA microarrays to outline the induction of several markers of lipid metabolism in the heart at birth in surviving mice. We hypothesized that the induction of fat metabolism genes in the heart at birth is part of a regulatory feedback circuit that plays a critical role in survival. The present study uses a dual approach employing both C57BL/6 mice and the nematode, C. elegans, to focus on TMEM135, a conserved protein which we have found to be upregulated 4.3 (±0.14)-fold in VLCAD-deficient mice at birth. Our studies have demonstrated that TMEM135 is highly expressed in mitochondria and in fat-loaded tissues in the mouse. Further, when fasting and cold stresses were introduced to mice, we observed 3.25 (±0.03)- and 8.2 (±0.31)-fold increases in TMEM135 expression in the heart, respectively. Additionally, we found that deletion of the tmem135 orthologue in C. elegans caused a 41.8% (±2.8%) reduction in fat stores, a reduction in mitochondrial action potential and decreased longevity of the worm. In stark contrast, C. elegans transgenic animals overexpressing TMEM-135 exhibited increased longevity upon exposure to cold stress. Based on these results, we propose that TMEM135 integrates biological processes involving fat metabolism and energy expenditure in both the worm (invertebrates) and in mammalian organisms. The data obtained from our experiments suggest that TMEM135 is part of a regulatory circuit that plays a critical role in the survival of VLCAD-deficient mice and perhaps in other mitochondrial genetic defects of fat metabolism as well.     

Fluorescence-based fixative and vital staining of lipid droplets in Caenorhabditis elegans reveal fat stores using microscopy and flow cytometry approaches

The proportions of body fat and fat-free mass are determining factors of adiposity-associated diseases. Work in Caenorhabditis elegans has revealed evolutionarily conserved pathways of fat metabolism. Nevertheless, analysis of body composition and fat distribution in the nematodes has only been partially unraveled because of methodological difficulties. We characterized metabolic C. elegans mutants by using novel and feasible BODIPY 493/503-based fat staining and flow cytometry approaches. Fixative as well as vital BODIPY staining procedures visualize major fat stores, preserve native lipid droplet morphology, and allow quantification of fat content per body volume of individual worms. Colocalization studies using coherent anti-Stokes Raman scattering microscopy, Raman microspectroscopy, and imaging of lysosome-related organelles as well as biochemical measurement confirm our approaches. We found that the fat-to-volume ratio of dietary restriction, TGF-β, and germline mutants are specific for each strain. In contrast, the proportion of fat-free mass is constant between the mutants, although their volumes differ by a factor of 3. Our approaches enable sensitive, accurate, and high-throughput assessment of adiposity in large C. elegans populations at a single-worm level.     

lncRNA在动物脂肪沉积中的研究进展*

长链非编码RNA(long non-coding RNA,lncRNA),是一种长度大于200个核苷酸的调控性非编码RNA,能在转录水平、转录后水平及表观遗传水平等多个层面影响基因的表达。脂肪生成是一个复杂而有序的过程。大量研究表明,lncRNA在脂肪生成过程中扮演着重要角色,它可以影响脂质代谢及成脂分化等多种生物过程,从而间接影响肉品质。这对于提高畜禽肉品质、避免养殖业饲料过多转化成脂肪所导致的浪费以及对预防和治疗与脂肪代谢相关的疾病都具有重要意义。对lncRNA的基本特征、在动物脂肪沉积中的作用进展进行了综述,以期为培育优质畜禽,预防和治疗与脂肪代谢相关的疾病提供理论依据。     

Identification and functional analysis of mitochondrial complex I assembly factor homologues in C. elegans

The biogenesis of mitochondrial NADH:ubiquinone oxidoreductase (complex I) requires several assembly chaperones. These so-called complex I assembly factors have emerged as a new class of human disease genes. Here, we identified putative assembly factor homologues in Caenorhabditis elegans. We demonstrate that two candidates (C50B8.3/NUAF-1, homologue of NDUFAF1 and R07H5.3/NUAF-3, homologue of NDUFAF3) clearly affect complex I function. Assembly factor deficient worms were shorter, showed a diminished brood size and displayed reduced fat content. Our results suggest that mitochondrial complex I biogenesis is evolutionarily conserved. Moreover, Caenorhabditis elegans appears to be a promising model organism to study assembly factor related human diseases.     

Direct measurement of oxygen consumption rate on the nematode Caenorhabditis elegans by using an optical technique

It is well known that aging and longevity strongly correlate with energy metabolism. The nematode Caenorhabditis elegans is widely used as an ultimate model of experimental animals. Thus, we developed a novel tool, which is constructed from an optical detector, using an indirect method that can measure simply the energy metabolism of C. elegans. If we measure the oxygen consumption rate using this optical tool, we can easily evaluate the activity of mitochondria as an index in the aging process. However, a direct measurement of the oxygen consumption rate of C. elegans exposed in air is thought to be impossible because of the high concentration of atmospheric oxygen and the small size of the animals. We demonstrate here that we can directly detect the oxygen consumption with a small number of animals (相似文献