健康学龄前儿童血浆游离氨基酸含量研究

采用丹酰氯薄膜层析法分析了６５名３～７岁健康儿童血浆游离氨基酸含量。结果表明：血浆１１种游离氨基酸含量未见性别年龄差异,其正常值可供临床实践应用。血浆游离氨基酸在健康组中显著高于残疾组,提示重度营养不良时,各种氨基酸含量显著下降。     

不同肝脏疾病患者血浆游离氨基酸水平改变的模式

采用DNS -Cl荧光标记氨基酸 ,聚酰胺薄膜层析 ,分析了慢性血吸虫病肝肿大、晚期血吸虫病肝肿大 ,慢活肝 ,慢迁肝以及肝硬化等五种不同肝损伤患者血浆游离氨基酸水平 ,结果发现它们血浆游离氨基酸改变 ,均有一定的特异的模式     

肺癌患者胸水游离氨基酸分析

本文对11例肺癌患者胸水13种游离氨基酸作了分析,并与28例正常人血浆游离氨基酸水平作了对照,结果表明:肺癌患者胸水的必需及非必需氨基酸普遍高于正常人血浆游离氨基酸,但其胸水谷氨酰胺水平则明显低于正常人血浆水平。     

健康学龄前儿童血浆氨基酸与血红蛋白含量

本文采用丹酰氯聚酰胺薄层分析法研究了６５名３—７岁健康儿童的血浆游离氨基酸和血红蛋白浓度。结果表明：（１）赖氨酸促进蛋白质合成不仅供机体生长需要,同样也供合成血红蛋白;（２）贫血前期已有血浆游离氨基酸的变化,继而出现贫血,提示儿童时期生长旺盛,应注重补充某些氨基酸消耗和补充维生素、叶酸、铁元素等造血原料。     

平衡型氨基酸透析液对维持性血液透析患者血浆游离氨基酸含量的影响

目的:研究一种平衡型氨基酸透析液,探讨其对维持性血透患者血游离氨基酸含量的影响。方法:实验采用自身对照设计,应用蛋白水解法测定、分析应用氨基酸透析液血透前后患者血浆氨基酸含量,并同时测定应用碳酸盐透析液患者及健康人血浆游离氨基酸作为比较。结果:经碳酸盐透析后,患者血浆大多数游离氨基酸和总氨基酸含量显著降低。给予平衡氨基酸透析液能不同程度改善患者血浆游离氨基酸的含量。结论:平衡型氨基酸透析液能减少血中部分氨基酸的丢失。     

烧伤患者血浆和红细胞游离氨基酸变化及输注氨基酸对其变化的影响

动态测定烧伤患者血浆及红细胞内游离氨基酸的含量 ,探讨输入外源性氨基酸后对血及红细胞内游离氨基酸的影响。以日立 835— 5 0型氨基酸自动分析仪测定烧伤患者血浆及红细胞内游离氨基酸含量。结果发现烧伤患者血浆总游离氨基酸浓度从伤后到 2 1天均显著降低 (P <0 .0 5～ 0 .0 1) ;赖、苯丙和苯丙 酪氨酸比值显著升高 (P <0 .0 5～ 0 .0 1) ;色、组、精、丙、甘、苏、脯和丝氨酸比值显著降低 (P <0 .0 5～ 0 .0 1) ;缬、亮、异亮、酪、胱和支链氨基酸伤后早期降低。烧伤患者红细胞内总游离氨基酸含量不同程度降低 ,其中 1、3、7天降低显著 (P <0 .0 5～ 0 .0 1) ;红细胞内苯丙和苯丙 酪氨酸比值未见显著性升高 ;色、蛋、精、脯氨酸含量很低或基本未测出。输注复合氨基酸注射液后未能显著改善患者血及红细胞内游离氨基酸含量。结果提示烧伤患者红细胞内游离氨基酸含量的变化趋势与血浆游离氨基酸变化趋势基本一致 ;烧伤后红细胞内苯丙氨酸及苯丙 酪氨酸比值有别于血浆变化。本研究条件下补充外源性氨基酸未能显著改变烧伤患者血浆及红细胞内游离氨基酸的含量     

血浆中氨基酸检测技术探讨

在血浆中检测游离氨基酸含量是临床诊断的重要手段之一。本文讨论了液相色谱法、氨基酸分析法、液质联用技术等几种血浆中氨基酸的检测技术,并介绍这些技术在氨基酸检测中的应用,为建立血浆中游离氨基酸的分析方法提供参考依据。     

支气管哮喘患者血浆游离氨基酸改变

<正> 支气管哮喘常与过敏因素有关,且易导致血管活性物质改变,最终造成代谢紊乱,为了探索哮喘患者氨基酸代谢的变化,确立哮喘患者血浆游离氨基酸模式及其在发病中的价值,我们对11例支气管哮喘患者及29例正常人作了血浆游离氨基酸测定,现将结果报道如下:     

多胺和氨基酸与儿童智能发育的研究

采用丹酰氯聚酰胺薄层分析法研究了３７名３～７岁健康儿童的红细胞多胺和血浆游离氨基酸,并采用“韦克斯勒学前儿童智力检查法”测试智能。结果表明：（１）红细胞多胺含量与学前儿童的大脑生长发育无明显影响,而支链氨基酸及苯丙氨酸、酪氨酸与大脑的功能活动及代谢有一定的作用。     

乙型脑炎患者脑脊液和血浆氨基酸代谢的变化

本文采用气相色谱法对５７例乙型脑炎患者极期和２１例恢复期患者的脑脊液（ＣＳＦ）和血浆１４种游离氨基酸浓度进行分析。结果表明,乙脑极期ＣＳＦ游离氨基酸浓度除苏氨酸、丝氨酸值低于正常值外,其它氨基酸值均增高,其中谷氨酸、鸟氨酸、赖氨酸、缬氨酸、脯氨酸值上升明显（ａｌｌＰ＜０．０１）,至乙脑恢复期不同幅度下降。乙脑极期血浆游离脯氨酸、赖氨酸、苯丙氨酸、酪氨酸、鸟氨酸、谷氨酸浓度高于正常值,而其它８种氨基酸值均下降明显,至乙脑恢复期,多数氨基酸恢复至接近正常水平。实验提示,乙脑病毒能明显影响血浆和ＣＳＦ中氨基酸的含量,而二者具有代谢差异。     

烫伤大鼠早期口服谷氨酰胺对血浆氨基酸代谢的影响

研究烫伤早期口服谷氨酰胺 (GLN)后GLN及其相关氨基酸的代谢变化。以Wistar大鼠为烫伤模型 ,烫伤后早期口服GLN ,并以 83 5-50型氨基酸自动分析仪的生理体液法测定血浆游离氨基酸。结果烫伤后饲标准饲料(C)组血浆GLN在烫伤后 2d和 5d降低 ,与GLN代谢相关的丙氨酸和氨无显著性变化 ;GLN饲料 (G)组各时相点GLN均增加 ,1 0h和 8d增加显著 ,与GLN代谢相关的丙氨酸和血氨增加显著 ;GLN +精氨酸 (G +A)组GLN在 2d降低 ,血氨升高显著。与C组比 ,G和G +A组血浆总氨基酸、支琏氨基酸、GLN、丙氨酸、r-氨基丁酸和氨均较C组高。提示 ,烫伤后早期口服GLN能提高血GLN和与之代谢相关的氨基酸浓度。     

Comparison of Protein Precipitants for the Determination of Free Amino Acids in Plasma

The present study was undertaken to select the most preferred deproteinizing agent for microbiological or chromatographic determination of free amino acids in plasma. The highest quantities of amino acids were obtained from samples treated with picric acid, ethanol or sulfosalicylic acid. It was also found from examining the recoveries of ¹⁴C-labeled amino acids added to plasma following treatment with picric acid, ethanol and sulfosalicylic acid that one kind of deproteinizing agent is not equally effective with all amino acids. Picric acid treatment gave the most reliable results for determination of amino acids except the basic amino acids, alanine and tryptophan. Sulfosalicylic acid was the most recommended deproteinizing agent for basic amino acids and ethanol was good for the assay of alanine and tryptophan. The response of d-amino acids to the deproteinizing agents tested was not always similar to that of their l-isomers.     

新型氨基酸制剂对创伤大鼠血游离氨基酸的影响

观察了富含牛磺酸 (Tau)、谷氨酰胺 (Gln)以及高支链氨基酸 (HBCAA)的新型氨基酸制剂对创伤大鼠血中游离氨基酸浓度的影响。结果表明 ,创伤后三天起 ,血浆游离氨基酸总和均显著降低 ,对照组基本无改变 ;创伤后Tau、BCAA、精氨酸以及天冬氨酸等具有抗氧化和免疫调节作用的氨基酸含量明显降低 ,新处方使用一周后其浓度有效回升 ,且效果好于 17种氨基酸 ,从而有利于机体伤口的愈合。这些结果为进一步阐明复合氨基酸制剂促进创伤愈合的作用及其开发应用提供了理论依据     

Age-related changes of muscle and plasma amino acids in healthy children

The aim of the study was to explore if changes in muscle and plasma amino acid concentrations developed during growth and differed from levels seen in adults. The gradient and concentrations of free amino acids in muscle and plasma were investigated in relation to age in metabolic healthy children. Plasma and specimens from the abdominal muscle were obtained during elective surgery. The children were grouped into three groups (group 1: < 1 year, n = 8; group 2: 1–4 years, n = 13 and group 3: 5–15 years, n = 15). A reference group of healthy adults (21–38 years, n = 22) was included in their comparisons and reflected specific differences between children and adults. In muscle the concentrations of 8 out of 19 amino acids analysed increased with age, namely taurine, aspartate, threonine, alanine, valine, isoleucine, leucine, histidine, as well as the total sums of branched chain amino acids (BCAA), basic amino acids (BAA) and total sum of amino acids (P < 0.05). In plasma the concentrations of threonine, glutamine, valine, cysteine, methionine, leucine, lysine, tryptophane, arginine, BCAA, BAA and the essential amino acids correlated with age (P < 0.05). These results indicate that there is an age dependency of the amino acid pattern in skeletal muscle and plasma during growth.     

Impact of potassium,sodium, and salinity on the protein-and free amino acid content of wheat grain

A lysimeter study was conducted on Cajeme wheat (Triticum aestivum L.) to investigate the impact of salinity on protein and free amino acid content of the grain. Cross correlations were obtained between 16 different soil-plant-water based parameters and the concentration and total accumulation of amino acids. The results indicated that after 3 years of irrigation, the majority of protein bound and free amino acids increased in concentration in the grain. However, both free tryptophan and free proline revealed decreasing concentrations with increasing salinity. Free tryptophan showed a synergism between total accumulation, yield and concentration. Free proline concentrations decreased in association with increasing protein concentrations. Cross correlations of the 16 soil-plant-water based parameters with free and protein bound amino acids revealed significant correlations for free aspartic acid and glycine with total accumulation but not with concentrations. Only methionine plus cystine was lower than suggested FAO levels for essential amino acids and was lower in the third year than in the first year.     

实验动物血及组织游离氨基酸含量

本文报告实验动物血及组织游离氨基酸含量,结果表明,实验狗和Wistar大鼠血游离氨基酸含量比较恒定。实验兔组织游离氨基酸含量普遍高于血。     

Comparative Analysis of the Amino Acid Spectrum of Blood Plasma in Chiroptera (<Emphasis Type="Italic">Vespertilio murinus</Emphasis> L., 1758 and <Emphasis Type="Italic">Myotis dasycneme</Emphasis> B., 1825) in the Fauna of the Ural Mountains

The article presents the results of a new comparative analysis of free amino acids in the blood plasma of representatives of insectivorous Chiroptera (Mammalia: Vespertilionidae) in the fauna of the Ural Mountains: the pond bat (Myotis dasycneme Boie, 1825) and the parti-colored bat (Vespertilio murinus Linnaeus, 1758). This is the first study to show the species variability of free amino acids in resident and migratory species of bats from different ecosystems of the Ural Region.     

Extra- and intracellular amino acids in the hippocampus during development of hepatic encephalopathy

Fulminant hepatic failure was induced in rabbits by intravenous administration of galactosamine hydrochloride. The animals were sacrified after 45 h and the hippocampus analyzed for free amino acids. In addition, free amino acids were measured in plasma and in the extracellular fluid of the hippocampus 20, 30 and 45 h after galactosamine injection. The extracellular fluid compartment was analyzed by slow perfusion of a thin dialysis tube which was implanted in the hippocampus one day prior to galactosamine administration. The amino acid concentration in the extracellular fluid agreed fairly well with that of the cerebrospinal fluid in the control situation. During development of hepatic failure, the plasma concentrations of all amino acids increased. The changes in extracellular amino acids were smaller, except for phosphoethanolamine and glutamate. The concentration ratio intra/extracellular amino acids decreased in the hippocampus for amino acids with a normally high concentration gradient.