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1.
Leaf photosynthesis, plant growth and nitrogen allocation in rice under different irradiances 总被引:6,自引:0,他引:6
The photosynthetic rates and various components of photosynthesis including ribulose-1,5-bisphosphate carboxylase (Rubisco;
EC 4.1.1.39), chlorophyll (Chl), cytochrome (Cyt) f, and coupling factor 1 (CF1) contents, and sucrose-phosphate synthase (SPS; EC 2.4.1.14) activity were examined in young, fully expanded leaves of rice
(Oryza sativa L.) grown hydroponically under two irradiances, namely, 1000 and 350 μmol quanta · m−2 · s−1, at three N concentrations. The light-saturated rate of photosynthesis measured at 1800 μmol · m−2 · s−1 was almost the same for a given leaf N content irrespective of growth irradiance. Similarly, Rubisco content and SPS activity
were not different for the same leaf N content between irradiance treatments. In contrast, Chl content was significantly greater
in the plants grown at 350 μmol · m−2 · s−1, whereas Cyt f and CF1 contents tended to be slightly smaller. However, these changes were not substantial, as shown by the fact that the light-limited
rate of photosynthesis measured at 350 μmol · m−2 · s−1 was the same or only a little higher in the plants grown at 350 μmol · m−2 · s−1 and that CO2-saturated photosynthesis did not differ between irradiance treatments. These results indicate that growth-irradiance-dependent
changes in N partitioning in a leaf were far from optimal with respect to N-use efficiency of photosynthesis. In spite of
the difference in growth irradiance, the relative growth rate of the whole plant did not differ between the treatments because
there was an increase in the leaf area ratio in the low-irradiance-grown plants. This increase was associated with the preferential
N-investment in leaf blades and the extremely low accumulation of starch and sucrose in leaf blades and sheaths, allowing
a more efficient use of the fixed carbon. Thus, morphogenic responses at the whole-plant level may be more important for plants
as an adaptation strategy to light environments than a response of N partitioning at the level of a single leaf.
Received: 23 February 1997 / Accepted: 8 May 1997 相似文献
2.
Intact leaves of kiwifruit (Actinidia deliciosa (A. Chev.) C.F. Liang et A.R. Ferguson) from plants grown in a range of controlled temperatures from 15/10 to 30/25°C were
exposed to a photon flux density (PFD) of 1500 μmol·m−2·s−1 at leaf temperatures between 10 and 25°C. Photoinhibition and recovery were followed at the same temperatures and at a PFD
of 20 μmol·m−2·s−1, by measuring chlorophyll fluorescence at 77 K and 692 nm, by measuring the photon yield of photosynthetic O2 evolution and light-saturated net photosynthetic CO2 uptake. The growth of plants at low temperatures resulted in chronic photoinhibition as evident from reduced fluorescence
and photon yields. However, low-temperature-grown plants apparently had a higher capacity to dissipate excess excitation energy
than leaves from plants grown at high temperatures. Induced photoinhibition, from exposure to a PFD above that during growth,
was less severe in low-temperature-grown plants, particularly at high exposure temperatures. Net changes in the instantaneous
fluorescence,F
0, indicated that little or no photoinhibition occurred when low-temperature-grown plants were exposed to high-light at high
temperatures. In contrast, high-temperature-grown plants were highly susceptible to photoinhibitory damage at all exposure
temperatures. These data indicate acclimation in photosynthesis and changes in the capacity to dissipate excess excitation
energy occurred in kiwifruit leaves with changes in growth temperature. Both processes contributed to changes in susceptibility
to photoinhibition at the different growth temperatures. However, growth temperature also affected the capacity for recovery,
with leaves from plants grown at low temperatures having moderate rates of recovery at low temperatures compared with leaves
from plants grown at high temperatures which had negligible recovery. This also contributed to the reduced susceptibility
to photoinhibition in low-temperature-grown plants. However, extreme photoinhibition resulted in severe reductions in the
efficiency and capacity for photosynthesis. 相似文献
3.
To test the hypothesis that the contribution of phosphoribulokinase (PRK) to the control of photosynthesis changes depending
on the light environment of the plant, the response of transgenic tobacco (Nicotiana tabacum L.) transformed with antisense PRK constructs to irradiance was determined. In plants grown under low irradiance (330 μmol m−2 s−1) steady-state photosynthesis was limited in plants with decreased PRK activity upon exposure to higher irradiance, with a
control coefficient of PRK for CO2 assimilation of 0.25 at and above 800 μmol m−2 s−1. The flux control coefficient of PRK for steady-state CO2 assimilation was zero, however, at all irradiances in plant material grown at 800 μmol m−2 s−1 and in plants grown in a glasshouse during mid-summer (alternating shade and sun 300–1600 μmol m−2 s−1). To explain these differences between plants grown under low and high irradiances, Calvin cycle enzyme activities and metabolite
content were determined. Activities of PRK and other non-equilibrium Calvin cycle enzymes fructose-1,6-bisphosphatase, sedoheptulose-1,7-bisphosphatase
and ribulose-1,5-bisphosphate carboxylase-oxygenase were twofold higher in plants grown at 800 μmol m−2 s−1 or in the glasshouse than in plants grown at 330 μmol m−2 s−1. Activities of equilibrium enzymes transketolase, aldolase, ribulose-5-phosphate epimerase and isomerase were very similar
under all growth irradiances. The flux control coefficient of 0.25 in plants grown at 330 μmol m−2 s−1 can be explained because low ribulose-5-phosphate content in combination with low PRK activity limits the synthesis of ribulose-1,5-bisphosphate.
This limitation is overcome in high-light-grown plants because of the large relative increase in activities of sedoheptulose-1,7-bisphosphatase
and fructose-1,6-bisphosphatase under these conditions, which facilitates the synthesis of larger amounts of ribulose-5-phosphate.
This potential limitation will have maintained evolutionary selection pressure for high concentrations of PRK within the chloroplast.
Received: 15 November 1999 / Accepted: 27 January 2000 相似文献
4.
Microstegium vimineum (Trin.) A. Camus, a shade-tolerant C4 grass, has spread throughout the eastern United States since its introduction in 1919. This species invades disturbed understory
habitats along streambanks and surrounding mesic forests, and has become a major pest in areas such as Great Smoky Mountains
National Park. The focus of this study was to characterize the photosynthetic induction responses of M. vimineum, specifically its ability to utilize low light and sunflecks, two factors that may be critical to invasive abilities and
survival in the understory. In addition, we were curious about the ability of a grass with the C4 photosynthetic pathway to respond to sunflecks. Plants were grown under 25% and 50% ambient sunlight, and photosynthetic
responses to both steady-state and variable light were determined. Plants grown in both 25% and 50% ambient sun became 90%
light saturated between 750–850 μmol m−2 s−1; however, plants grown in 50% ambient sun had significantly higher maximum steady-state photosynthetic rates (16.09 ± 1.37 μmol m−2 s−1 vs. 12.71 ± 1.18 μmol m−2 s−1). Both groups of plants induced to 50% of the steady-state rate in 3–5 min, while it took 10–13 min to reach 90% of maximum
rates, under both flashing and steady-state light. For both groups of plants, stomatal conductance during induction reached
maximum rates in 6–7 min, after which rates decreased slightly. Upon return to low light, rates of induction loss and stomatal
closure were very rapid in both groups of plants, but were more rapid in those grown in high light. Rapid induction and the
ability to induce under flashing light may enable this species to invade and dominate mesic understory habitats, while rapid
induction loss due to stomatal closure may prevent excess water loss when low light constrains photosynthesis. The C4 pathway itself does not appear to present an insurmountable barrier to the ability of this grass species to respond to sunflecks
in an understory environment.
Received: 21 February 1997 / Accepted: 10 October 1997 相似文献
5.
L. E. M. Nery M. A. da Silva A. M. Lauro Castrucci 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1998,168(8):624-630
The participation of cyclic nucleotide-dependent intracellular signalling pathways in the pigment translocation induced by
pigment-dispersing hormone (α -PDH) or pigment-concentrating hormone (PCH) was investigated in the erythrophores of the freshwater
shrimp, Macrobrachium potiuna. Cholera toxin, forskolin and dibutyryl cyclic adenosine 3′5′ monophosphate (dbcAMP) were able to induce pigment dispersion
with effective agonist concentrations for half maximal response (EC50 s) of 2.8 · 10−11 mol · l−1, 7.0 · 10−7 mol · l−1 and 3.3 · 10−7 mol · l−1, respectively. KT5720 (10−7 mol · l−1 and 10−6 mol · l−1) significantly shifted the dose response curve to α -PDH to the right. Dibutyryl cyclic guanosine 3′5′ monophosphate (dbcGMP)
was ineffective in inducing either pigment aggregation or dispersion. 2′5′ dideoxyadenosine (DDA) and SQ22,536 essentially
elicit a pigment-aggregating response in a dose-dependent manner. These effects were not due to the activation of purinergic
receptors, since concentrations up to 10−4 mol · l−1 of adenosine and adenosine triphosphate (ATP), and up to 10−3 mol · l−1 of uracil triphosphate (UTP) did not elicit pigment aggregation. In order to verify if PCH decreased cyclic adenosine 3′5′
monophosphate (cAMP) levels, cumulative dose-response curves to PCH in the absence and presence of pertussis toxin and 8-MOM-IBMX
were determined. However, neither drug significantly affected PCH activity. The levels of cAMP in the integument cells of
M. potiuna were significantly increased (P < 0.05) by α -PDH (10−7 mol · l−1) and forskolin (10−6 mol · l−1), but were not affected by PCH (10−7 or 10−10 mol · l−1). In conclusion, α -PDH seems to elicit pigment dispersion through the activation of a Gs-protein coupled receptor resulting
in cAMP increase and cAMP-dependent protein kinase (PKA) activation. Furthermore, although a decrease in cAMP was assumed
to be responsible in turn for the action of PCH, such a decrease could not be directly demonstrated.
Accepted: 11 August 1998 相似文献
6.
Dennis H. Greer 《Planta》1995,197(1):31-38
Bean (Phaseolus vulgaris L.) plants were grown at two light periods of 8 and 13 h with a similar photon flux density (PFD) giving a daily photon receipt (DPR) of 17.9 and 38.2 mol · m–2, respectively. Shoot growth and leaf area development were followed at regular intervals and diurnal whole-plant photosynthesis measured. Single mature trifoliate leaves were exposed to photoinhibitory treatments at PFDs of 800 and 1400 mol · m–2 · s–1 and at temperatures of 12 and 20°C. Chlorophyll fluorescence and photon yields were measured at regular intervals throughout each treatment. Plants grown in 13 h had significantly greater leaf areas than those grown in 8 h. There were no differences in maximum rates of photosynthesis, photon yields and only minor but significant differences in Fv/Fm for plants in the two treatments, showing photosynthetic characteristics were dependent on PFD but not DPR. A significant decline in photosynthesis and Fv/Fm occurred over the 13-h but little change in photosynthesis for plants in the 8 h, indicating some feedback inhibition of photosynthesis was occurring. Plants grown in 8 h were consistently more susceptible to photoinhibition of photosynthesis at all treatments than 13-h plants. Nevertheless, photoinhibition was exacerbated by increases in PFD, and by decreases in temperature for leaves from both treatments. However, for plants from the 8-h day, exposing leaves to 12°C and 1400 mol · m–2 · s–1 caused photo-oxidation and severe bleaching but no visible damage on leaves from 13-h-grown plants. Closure of the photosystem II reaction-centre pool was partially correlated with increasing extents of photoinhibition but the relationship was similar for plants from both treatments. There remains no clear explanation for their wide differences in susceptibility to photoinhibition.Abbreviations and Symbols DPR
daily photon receipt
- F0 and Fm
initial and maximal fluorescence
- Fv/Fm
fluorescence ratio in dark-treated leaves
- F/Fm
intrinsic efficiency of PSII during illumination
- PFD
photon flux density
-
i
photon yield (incident basis)
-
psi
quantum yield of PSII electron transport
- Pmax
maximum rate of photosynthesis
- qN
non-photochemical quenching coefficient
- qP
photochemical quenching coefficient
Many thanks to my colleague William Laing who spent a considerable effort in developing the programme to run the photosynthesis apparatus. I am also indebted to one reviewer with whom I corresponded to resolve some issues in the paper. This project was funded by the New Zealand Foundation for Research, Science and Technology. 相似文献
7.
Eun-A. Jo Rajesh Kumar Tewari Eun-Joo Hahn Kee-Yoeup Paek 《Plant biotechnology reports》2008,2(3):207-212
Plantlets of Alocasia amazonica regenerated under a photon flux density (PFD) of 15 or 30 μmol m−2 s−1 showed better growth and development than those grown under higher PFDs. While chlorophyll a and chlorophyll b decreased, the number of stomata increased with increasing PFD. Photoperiods also affected plantlet growth and stomatal development.
Highest growth was observed for the short photoperiod (8/16 h) and for equinoctial (12/12 h) light and dark periods. Very
few stomata developed in the leaves of plantlets grown under a short photoperiod (8/16 h) and the number of stomata increased
with increasing light period. In conclusion, both light intensity and photoperiod independently affect growth of A. amazonica and development of stomata, depending on the intensity and duration of light treatment. 相似文献
8.
Exposure of barley (Hordeum vulgare L.) leaves to strong white light (1500 μmol photons · m−2 · s−1) decreased the quantum yield of photosynthetic oxygen evolution in green light preferentially absorbed by carotenoids (Φ-510)
but not in red light exclusively absorbed by chlorophylls (Φ-650). This phenomenon was observed to be (i) rapidly induced
(within a few minutes), (ii) slowly reversible in darkness (within about 1 h), (iii) insensitive to dithiothreitol and (iv)
maximally induced by photon flux densities higher than about 1000 μmol · m−2 · s−1. Determination of the carotenoid composition of the major light-harvesting complex of PSII (LHCII) and analysis of the thylakoid
membrane lipid fluidity before and after strong illumination of barley leaves in the presence or the absence of dithiothreitol
showed that the light-induced decrease in the Φ-510/Φ-650 ratio did not require the physical detachment of carotenoids from
the pigment antennae. Compared to barley plants grown under moderate light and temperature conditions, plants grown in sustained
high irradiance at elevated temperature exhibited (i) a lower Φ-510/Φ-650 ratio, (ii) a reduced size of the functional PSII
pigment antenna in green light (but not in red light) and (iii) a marked increase in the amount of free carotenoids found
in non-denaturing Deriphat-containing electrophoretic gels of thylakoid membranes. Similarly, the Φ-510/Φ-650 ratio of the
LHCII-deficient chlorina-f2 barley mutant was very low compared to the wild type. Separation and quantification of the cis/trans carotenoid isomers of barley leaves revealed that strong illumination did not induce pronounced cis-trans isomerization of xanthophylls. Taken together, the data suggest that the efficiency of energy transfer from carotenoids to
chlorophylls varies with the light environment both in the short term and in the long term, with excess light energy noticeably
inhibiting the photosynthetic light-harvesting function of carotenoids. The photoprotective significance of this carotenoid
decoupling from the chlorophyll antennae is discussed.
Received: 28 July 1997 / Accepted: 25 October 1997 相似文献
9.
S. M. E. Guirguis J. C. Yee D. F. Stiffler 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1997,167(5):328-334
The skin of intact, free-swimming Xenopus laevis transports Ca2+ inwardly in a manner that is proportional to the external [Ca2+] up to about 0.3 mmol · l−1, saturates above 0.3 mmol · l−1, and is opposed to the electrochemical gradient. Efflux is relatively constant at external concentrations between 0.016 and
0.6 mmol · l−1; net flux which is negative below 0.125 mmol · l−1 becomes positive above this external [Ca2+]. Allometric analysis suggests that both Ca2+ influx and efflux scale to the 2/3 power approximately like surface area. There were no significant differences in influx
between summer and fall animals; however, efflux was greater in the fall and this resulted in a change from positive balance
in the summer to negative balance in the fall. Isolated skins were shown to support a Ca2+ uptake rate of nearly 30 nmol · cm−2 · h−1. The phenylalkylamine verapamil in the apical bathing solution significantly inhibited this at 25 μmol · l−1. The benzothiazepine diltiazem was also effective at 50 μmol · l−1 while the dihydropyradine nifedipine was ineffective up to 100 μmol · l−1. The inorganic ion La3+ was effective at blocking Ca2+ uptake at 300 μmol · l−1; Ni2+ was also effective at 500 μmol · l−1 but Co2+ was ineffective up to 500 μmol · l−1. These results suggest that apical calcium channels in Xenopuslaevis skin have properties similar to mammalian L-channels and fish gill Ca2+ channels.
Accepted: 23 January 1997 相似文献
10.
The objective of the present study was to assess whether, in barley, nitrogen supply limits the rate of leaf elongation through
a reduction in (relative) cell elongation rate and whether this is attributable to a reduced turgor, a reduced availability
of osmolytes or, by implication, changed wall properties. Plants were grown on full-strength Hoagland solution (“Hoagland”-plants),
or on N-deficient Hoagland solution while receiving N at a relative addition rate of 16 or 8% N · plant-N−1 · d−1 (“16%-” and “8%-plants”). Hoagland-plants were demand-limited, whereas 16%- and 8%-plants were supply-limited in N. Third
leaves were analysed for leaf elongation rate and final epidermal cell length, and, within the basal growing region, for the
spatial distribution of relative segmental elongation rates (RSER, pin-pricking method), epidermal cell turgor (cell-pressure
probe), osmotic pressure (OP, picolitre osmometry) and water potential (Ψ). During the development of the third leaf, plants
grew at relative growth rates (relative increase in fresh weight ) of 18.2, 15.6 and 8.1% · d−1 (Hoagland-, 16%- and 8%-plants, respectively). Final leaf length and leaf elongation rate were highest in Hoagland plants
(ca. 34.1 cm and 2.33–2.60 mm · h−1, respectively), intermediate in 16%- plants (31.0 cm and 1.89–1.96 mm · h−1) and lowest in 8%-plants (29.4 cm and 1.41–1.58 mm · h−1). These differences were accompanied by only small differences in final cell length, but large differences in cell-flux rates
(146, 187 and 201 cells · cell-file−1 · d−1 in 8%-, 16%- and Hoagland-plants, respectively). The length of the growth zone (32–38 mm) was not much affected by N-levels
(and nutrient technique). A decrease in RSER in the growth zone distal to 10 mm produced the significant effect of N-levels
on leaf elongation rate. In all treatments, cell turgor was almost constant throughout the growing region, as were cell OP
and Ψ in 16%- and 8%-plants. In Hoagland-plants, however, cell OP increased by ca. 0.1 MPa within the zone of highest elongation
rates and, as a consequence, cell Ψ decreased simultaneously by 0.1 MPa. Cell Ψ increased considerably where elongation ceased.
Within the zone where differences in RSERs were highest between treatments (10–34 mm from base) average turgor was lowest,
OP highest and Ψ most negative in Hoagland- compared to 8%- and 16%-plants (P < 0.001), but not significantly different between 8%- and 16%-plants.
Received: 9 January 1997 / Accepted: 6 March 1997 相似文献
11.
Two 60-day experiments were conducted to study the influence of photon flux density (PFD) and temperature on the attachment
and development of Gloiopeltis tenax and Gloiopeltis furcata tetraspores. In the first experiment, tetraspores of the two Gloiopeltis species were incubated at five temperature ranges (8°C, 12°C, 16°C, 20°C, 24°C) under a constant PFD of 80 μmol photons m−2 s−1 with a photoperiod of 12:12. In a second experiment, tetraspores were incubated under five PFD gradients (30, 55, 80, 105,
130 μmol photons m−2 s−1) at a constant temperature of 16°C with a photoperiod of 12:12. Maximum density of attached tetraspores was observed at 16°C
for both species. Maximum per cent of spore germinating into disc was recorded at 12–16°C for G. tenax and 8–12°C for G. furcata. Maximum per cent of discs producing erect axes for G. tenax and G. furcata were recorded at 24°C and 20°C, respectively. Light had no significant effect on tetraspore attachment and developing into
disc, but it affected the growth, sprouting and survival of its discs. Under 30–55 μmol photons m−2 s−1, the discs of the two species of Gloiopeltis did not form thallus until the end of the experiment. Optimum PFD range for G. tenax discs was 80–105 μmol photons m−2 s−1, whilst it was 80–130 μmol photons m−2 s−1 for G. furcata. Results presented in this study are expected to assist the progress of artificial seeding of Gloiopeltis. 相似文献
12.
Lolium temulentum L. Ba 3081 was grown hydroponically in air (350 μmol mol−1 CO2) and elevated CO2 (700 μmol mol−1 CO2) at two irradiances (150 and 500 μmol m−2 s−1) for 35 days at which point the plants were harvested. Elevated CO2 did not modify relative growth rate or biomass at either irradiance. Foliar carbon-to-nitrogen ratios were decreased at elevated
CO2 and plants had a greater number of shorter tillers, particularly at the lower growth irradiance. Both light-limited and light-saturated
rates of photosynthesis were stimulated. The amount of ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) protein was
increased at elevated CO2, but maximum extractable Rubisco activities were not significantly increased. A pronounced decrease in the Rubisco activation
state was found with CO2 enrichment, particularly at the higher growth irradiance. Elevated-CO2-induced changes in leaf carbohydrate composition were small in comparison to those caused by changes in irradiance. No CO2-dependent effects on fructan biosynthesis were observed. Leaf respiration rates were increased by 68% in plants grown with
CO2 enrichment and low light. We conclude that high CO2 will only result in increased biomass if total light input favourably increases the photosynthesis-to-respiration ratio.
At low irradiances, biomass is more limited by increased rates of respiration than by CO2-induced enhancement of photosynthesis.
Received: 23 February 1999 / Accepted: 15 June 1999 相似文献
13.
Gas-exchange measurements were performed to analyze the leaf conductances and assimilation rates of potato (Solanum tuberosum L. cv. Desireé) plants expressing an antisense construct against chloroplastic fructose-1,6-bisphosphatase (FBPase, EC 3.1.3.11)
in response to increasing photon flux densities, different relative air humidities and elevated CO2 concentrations. Assimilation rates (A) and transpiration rates (E) were observed during a stepwise increase of photon flux
density. These experiments were carried out under atmospheric conditions and in air containing 500 μmol mol−1 CO2. In both gas atmospheres, two levels of relative air humidity (60–70% and 70–80%) were applied in different sets of measurements.
Intercellular CO2 concentration, leaf conductance, air-to-leaf vapour pressure deficit, and instantaneous water-use efficiency (A/E) were determined.
As expected, assimilation rates of the FBPase antisense plants were significantly reduced as compared to the wild type. Saturation
of assimilation rates in transgenic plants occurred at a photon flux density of 200 μmol m−2 s−1, whereas saturation in wild type plants was observed at 600 μmol m−2 s−1. Elevated ambient CO2 levels did not effect assimilation rates of transgenic plants. At 70–80% relative humidity and atmospheric CO2 concentration the FBPase antisense plants had significantly higher leaf conductances than wild-type plants while no difference
emerged at 60–70%. These differences in leaf conductance vanished at elevated levels of ambient CO2. Stomatal response to different relative air humidities was not affected by mesophyll photosynthetic activity. It is suggested
that the regulation of stomatal opening upon changes in photon flux density is merely mediated by a signal transmitted from
mesophyll cells, whereas the intercellular CO2 concentration plays a minor role in this kind of stomatal response. The results are discussed with respect to stomatal control
by environmental parameters and mesophyll photosynthesis.
Received: 24 September 1998 / Accepted: 9 February 1999 相似文献
14.
Protective effects of prostaglandins in the isolated gastric mucosa of the eel, Anguilla anguilla 总被引:1,自引:0,他引:1
Faggio C Denaro MG Lionetto MG Trischitta F 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2000,170(5-6):357-363
The protective effect of endogenous prostaglandins on the fish gastric mucosa was evaluated by studying the effect of indomethacin
and aspirin, known cyclooxigenase inhibitors, on the mucosal ulceration in the isolated gastric sacs of Anguilla anguilla. Gastric sacs devoid of muscle layers were incubated in the presence of indomethacin (10−4 mol · l−1) or aspirin (10−4 mol · l−1) in different experimental conditions. Both the anti-inflammatory drugs produced ulcers, but the effects were more severe
in the presence of histamine and in the absence of HCO3
− in the incubation bath. The effects of prostaglandin E2 (PGE2) on acid secretion rate (JH) and on alkaline secretion rate (JOH) were evaluated (with the aid of the pH stat method) in isolated gastric mucosa mounted in Ussing chambers. We found that
PGE2 (10−8–10−5 mol · l−1) increased JH in a dose-dependent manner. In tissues pretreated with luminal omeprazole (10−4 mol · l−1), PGE2 stimulated gastric alkaline secretion. It was nullified by serosal removal of HCO3
− or Na+ and by serosal ouabain (10−4 mol · l−1). These results suggested that prostaglandins also exert their protective effects in fish gastric mucosa. This protection
seems partially due to a stimulation of exogenous HCO3
− transport from the serosal to the mucosal side. It is likely that this transport is an active transcellular mechanism coupled
to Na+ transport.
Accepted: 14 April 2000 相似文献
15.
16.
The effects of different wavebands of UV radiation on photosynthesis and the expression and abundance of photosynthetic proteins
in oilseed rape (Brassica napus L. cv. Rebel) were investigated. Plants were grown outdoors under natural radiation (52° N, 0° E) supplemented with six wavebands
of UV radiation (0.4 Wm−2) between 313 nm and 356 nm. A control treatment was centred at 343 nm. Exposure to supplementary UV-A radiation (320–400
nm) had no significant effects, however UV-B radiation, centred at 313 nm, caused a marked reduction in photosynthesis. This
decrease was related to a reduction in the initial carboxylation velocity of Rubisco which was further correlated with a large
reduction in the expression and abundance of both large and small subunits of Rubisco. These results indicate a molecular
mechanism behind UV-B induced reductions in photosynthesis per unit area in plants grown under field conditions.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
17.
Theodore J. Angelopoulos Rebecca Lewis Thanassis Jamurtas Chris Schumann 《European journal of applied physiology and occupational physiology》1998,77(6):556-559
We characterized the effect of ten days of training on lipid metabolism in 6 [age 37.2 (2.3) years] sedentary, obese [BMI
34.4 (3.0) kg · m−2] males with normal glucose tolerance. An oral glucose tolerance test was performed prior to and at the end of the 10 d of
training period. The duration of each daily exercise session was 40 min at an intensity equivalent to ˜75% of the age predicted
maximum heart rate. Blood measurements were performed after an overnight fast, before and at the end of the 10 d period. Plasma
triacylglycerol was significantly (p < 0.05) reduced following exercise training (2.15 ± 0.29 vs. 1.55 ± 0.28 mmol · l−1). Very low density lipoprotein-triacylglycerol was also significantly (p < 0.05) reduced (1.82 ± 0.3 vs. 1.29 ± 0.29 mmol · l−1). No significant changes in high density lipoprotein-cholesterol were observed as a result of training. Following training
fasting plasma glucose and fasting plasma insulin were significantly reduced [Glucose: 5.9 (0.2) mmol · l−1 vs. 5.3 (0.22) mmol · l−1 (p < 0.05); Insulin 264.3 (53.8) ρ · mol · l−1 vs. 200.9 (30.1) ρ · mol · l−1, p = 0.05]. The total area under the glucose curve during the OGTT decreased significantly (p < 0.05). These preliminary data suggest that short-term exercise, without concomitant loss of body mass, induces favorable
changes in plasma triacylglycerol, and very low density lipoprotein-triacylglycerol and glucose tolerance but has no effect
on high density lipoproteincholesterol.
Accepted: 7 January 1998 相似文献
18.
The effects of temperature on photosynthesis of a rosette plant growing at ground level, Acaena cylindrostachya R. et P., and an herb that grows 20–50 cm above ground level, Senecio formosus H.B.K., were studied along an altitudinal gradient in the Venezuelan Andes. These species were chosen in order to determine
– in the field and in the laboratory – how differences in leaf temperature, determined by plant form and microenvironmental
conditions, affect their photosynthetic capacity. CO2 assimilation rates (A) for both species decreased with increasing altitude. For Acaena leaves at 2900 m, A reached maximum values above 9 μmol m−2 s−1, nearly twice as high as maximum A found at 3550 m (5.2) or at 4200 m (3.9). For Senecio leaves, maximum rates of CO2 uptake were 7.5, 5.8 and 3.6 μmol m−2 s−1 for plants at 2900, 3550 and 4200 m, respectively. Net photosynthesis-leaf temperature relations showed differences in optimum
temperature for photosynthesis (A
o.t.) for both species along the altitudinal gradient. Acaena showed similar A
o.t. for the two lower altitudes, with 19.1°C at 2900 m and 19.6°C at 3550 m, while it increased to 21.7°C at 4200 m. Maximum
A for this species at each altitude was similar, between 5.5 and 6.0 μmol m−2 s−1. For the taller Senecio, A
o.t. was more closely related to air temperatures and decreased from 21.7°C at 2900 m, to 19.7°C at 3550 m and 15.5°C at 4200 m.
In this species, maximum A was lower with increasing altitude (from 6.0 at 2900 m to 3.5 μmol m−2 s−1 at 4200 m). High temperature compensation points for Acaena were similar at the three altitudes, c. 35°C, but varied in Senecio from 37°C at 2900 m, to 39°C at 3550 m and 28°C at 4200 m. Our results show how photosynthetic characteristics change along
the altitudinal gradient for two morphologically contrasting species influenced by soil or air temperatures.
Received: 5 July 1997 / Accepted: 25 October 1997 相似文献
19.
T. J. Vasankari U. M. Kujala H. Rusko S. Sarna M. Ahotupa 《European journal of applied physiology and occupational physiology》1997,75(5):396-399
We investigated the effect of training and racing at moderate altitude (MA) on oxidative stress by assessment of serum diene
conjugation (DC) and serum antioxidant potential (TRAP). Nine male top level skiers were studied during a national race (20–30
km) at sea level (SL). Thereafter, the athletes trained for 2 weeks at MA, after which they participated in a 20–30 km race
at MA. Venous blood samples were taken before and after the race. The DC, indicating early events of lipid peroxi dation,
did not change during the race at SL (16 850 vs 15 900 ΔAbsorbance · l−1) or at MA (19 870 vs. 20 630 ΔAbs · l−1). At MA serum DC was higher than at SL both before (25%) and after (30%) the race, the postrace difference being statistically
significant (P < 0.05). The TRAP increased during the race at MA (from 1387 to 1943 μmol · 1−1, P = 0.016), but not at SL (1713 vs 1582 μmol · l−1). These observations would suggest that the level of oxidative stress might be greater during living, training and racing
at MA (higher DC levels). Increased TRAP during the race at MA may indicate that the physiological adaptation to extreme acute
oxidative stress was altered. The physiological significance of this observation remains to be investigated.
Accepted: 18 October 1996 相似文献
20.
Active excretion of ammonia across the gills of the shore crab Carcinus maenas and its relation to osmoregulatory ion uptake 总被引:1,自引:0,他引:1
D. Weihrauch W. Becker U. Postel S. Riestenpatt D. Siebers 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1998,168(5):364-376
The mechanism of transbranchial excretion of total ammonia of brackish-water acclimated shore crabs, Carcinus maenas was examined using isolated, perfused gills. Applying physiological gradients of NH4Cl (100–200 μmol · l−1) directed from the haemolymph space to the bath showed that the efflux of total ammonia consisted of two components. The
saturable component (excretion of NH4
+) greatly exceeded the linear component (diffusion of NH3). When an outwardly directed gradient (200 μmol · l−1) was applied, total ammonia in the perfusate was reduced by more than 50% during a single passage of saline through the gill.
Effluxes of ammonia along the gradient were sensitive to basolateral dinitrophenol, ouabain, and Cs+ and to apical amiloride. Acetazolamide (1 mmol · l−1 basolateral) or Cl−-free conditions had no substantial effects on ammonia flux, which was thus independent of both carbonic anhydrase mediated
pH regulation and osmoregulatory NaCl uptake. When an inwardly directed gradient (200 μmol · l−1) was employed, influx rates were about 10-fold smaller and unaffected by basolateral ouabain (5 mmol · l−1) or dinitrophenol (0.5 mmol · l−1). Under symmetrical conditions (100 μmol · l−1 NH4Cl on both sides) ammonia was actively excreted against the gradient of total ammonia, which increased strongly during the
experiment and against the gradient of the partial pressure of NH3. The active excretion rate was reduced to 7% of controls by basolateral dinitrophenol (0.5 mmol · l−1), to 44% by basolateral ouabain (5 mmol · l−1), to 46% by Na+-free conditions and to 42% by basolateral Cs+ (10 mmol · l−1), indicating basolateral membrane transport of NH4
+ via the Na+/K+-ATPase and K+-channels and a second active, apically located, Na+ independent transport mechanism of NH4
+. Anterior gills, which are less capable of active ion uptake than posterior gills, exhibited even increased rates of active
excretion of ammonia. We conclude that, under physiological conditions, branchial excretion of ammonia is a directed process
with a high degree of effectiveness. It even allows active extrusion against an inwardly directed gradient, if necessary.
Accepted: 11 March 1998 相似文献