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1.
Qiaojun Jia Jingjuan Zhang Sharon Westcott Xiao-Qi Zhang Mathew Bellgard Reg Lance Chengdao Li 《Functional & integrative genomics》2009,9(2):255-262
The barley sdw1/denso gene not only controls plant height but also yield and quality. The sdw1/denso gene was mapped to the long arm of chromosome 3H. Comparative genomic analysis revealed that the sdw1/denso gene was located in the syntenic region of the rice semidwarf gene sd1 on chromosome 1. The sd1 gene encodes a gibberellic acid (GA)-20 oxidase enzyme. The gene ortholog of rice sd1 was isolated from barley using polymerase chain reaction. The barley and rice genes showed a similar gene structure consisting
of three exons and two introns. Both genes share 88.3% genomic sequence similarity and 89% amino acid sequence identity. A
single nucleotide polymorphism was identified in intron 2 between barley varieties Baudin and AC Metcalfe with Baudin known
to contain the denso semidwarf gene. The single nucleotide polymorphism (SNP) marker was mapped to chromosome 3H in a doubled haploid population
of Baudin × AC Metcalfe with 178 DH lines. Quantitative trait locus analysis revealed that plant height cosegregated with
the SNP. The sdw1/denso gene in barley is the most likely ortholog of the sd1 in rice. The result will facilitate understanding of the molecular mechanism controlling semidwarf phenotype and provide
a diagnostic marker for selection of semidwarf gene in barley.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
2.
Guo Tai Yu Richard D. Horsley Bingxin Zhang Jerome D. Franckowiak 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(4):853-861
Semi-dwarfing genes have been widely used in spring barley (Hordeum vulgare L.) breeding programs in many parts of the world, but the success in developing barley cultivars with semi-dwarfing genes
has been limited in North America. Exploiting new semi-dwarfing genes may help in solving this dilemma. A recombinant inbred
line population was developed by crossing ZAU 7, a semi-dwarf cultivar from China, to ND16092, a tall breeding line from North
Dakota. To identify quantitative trait loci (QTL) controlling plant height, a linkage map comprised of 111 molecular markers
was constructed. Simple interval mapping was performed for each of the eight environments. A consistent QTL for plant height
was found on chromosome 7HL. This QTL is not associated with maturity and rachis internode length. We suggest the provisional
name Qph-7H for this QTL. Qph-7H from ZAU 7 reduced plant height to about 3/4 of normal; thus, Qph-7H is considered a semi-dwarfing gene. Other QTLs for plant height were found, but their expression was variable across the
eight environments tested. 相似文献
3.
Maria von Korff Huajun Wang Jens Léon Klaus Pillen 《Molecular breeding : new strategies in plant improvement》2008,21(1):81-93
Malting quality is genetically determined by the complex interaction of numerous traits which are expressed prior to and,
in particular, during the malting process. Here, we applied the advanced backcross quantitative trait locus (AB-QTL) strategy
(Tanksley and Nelson, Theor Appl Genet 92:191–203, 1996), to detect QTLs for malting quality traits and, in addition, to identify favourable exotic alleles for the improvement of
malting quality. For this, the BC2DH population S42 was generated from a cross between the spring barley cultivar Scarlett and the wild barley accession ISR42-8
(Hordeum vulgare ssp. spontaneum). A QTL analysis in S42 for seven malting parameters measured in two different environments yielded 48 QTLs. The exotic genotype
improved the trait performance at 18 (37.5%) of 48 QTLs. These favourable exotic alleles were detected, in particular, on
the chromosome arms 3HL, 4HS, 4HL and 6HL. The exotic allele on 4HL, for example, improved α-amylase activity by 16.3%, fermentability
by 0.8% and reduced raw protein by 2.4%. On chromosome 6HL, the exotic allele increased α-amylase by 16.0%, fermentability
by 1.3%, friability by 7.3% and reduced viscosity by 2.9%. Favourable transgressive segregation, i.e. S42 lines exhibiting
significantly better performance than the recurrent parent Scarlett, was recorded for four traits. For α-amylase, fermentability,
fine-grind extract and VZ45 20, 16, 2 and 26 S42 lines, respectively, surpassed the recurrent parent Scarlett. The present
study hence demonstrates that wild barley does harbour valuable alleles, which can enrich the genetic basis of cultivated
barley and improve malting quality traits. 相似文献
4.
Krzysztof Mikołajczak Anetta Kuczyńska Paweł Krajewski Aneta Sawikowska Maria Surma Piotr Ogrodowicz Tadeusz Adamski Karolina Krystkowiak Andrzej G. Górny Michał Kempa Iwona Szarejko Justyna Guzy-Wróbelska Kornelia Gudyś 《Journal of applied genetics》2017,58(1):23-35
High-yielding capacity of the modern barley varieties is mostly dependent on the sources of semi-dwarfness associated with the sdw1/denso locus. The objective of the study was to identify quantitative trait loci (QTLs) associated with the plant height and yield potential of barley recombinant inbred lines (RILs) grown under various soil moisture regimes. The plant material was developed from a hybrid between the Maresi (European cv.) and CamB (Syrian cv.). A total of 103 QTLs affecting analysed traits were detected and 36 of them showed stable effects over environments. In total, ten QTLs were found to be significant only under water shortage conditions. Nine QTLs affecting the length of main stem were detected on 2H-6H chromosomes. In four of the detected QTLs, alleles contributed by Maresi had negative effects on that trait, the most significant being the QLSt-3H.1-1 in the 3H.1 linkage group. The close linkage between QTLs identified around the sdw1/denso locus, with positive alleles contributed by Maresi, indicates that the semi-dwarf cv. Maresi could serve as a donor of favourable traits resulting in grain yield improvement, also under water scarcity. Molecular analyses revealed that the Syrian cv. also contributed alleles which increased the yield potential. Available barley resources of genomic annotations were employed to the biological interpretation of detected QTLs. This approach revealed 26 over-represented Gene Ontology terms. In the projected support intervals of QGWSl-5H.3-2 and QLSt-5H.3 on the chromosome 5H, four genes annotated to ‘response to stress’ were found. It suggests that these QTL-regions may be involved in a response of plant to a wide range of environmental disturbances. 相似文献
5.
Identification and verification of QTLs for agronomic traits using wild barley introgression lines 总被引:2,自引:1,他引:1
Schmalenbach I Léon J Pillen K 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(3):483-497
A set of 39 wild barley introgression lines (hereafter abbreviated with S42ILs) was subjected to a QTL study to verify genetic
effects for agronomic traits, previously detected in the BC2DH population S42 (von Korff et al. 2006 in Theor Appl Genet 112:1221–1231) and, in addition, to identify new QTLs and favorable wild barley alleles. Each line within
the S42IL set contains a single marker-defined chromosomal introgression from wild barley (Hordeum vulgare ssp. spontaneum), whereas the remaining part of the genome is exclusively derived from elite spring barley (H. vulgare ssp. vulgare). Agronomic field data of the S42ILs were collected for seven traits from three different environments during the 2007 growing
season. For detection of putative QTLs, a two-factorial mixed model ANOVA and, subsequently, a Dunnett test with the recurrent
parent as a control were conducted. The presence of a QTL effect on a wild barley introgression was accepted, if the trait
value of a particular S42IL was significantly (P < 0.05) different from the control, either across all environments and/or in a particular environment. A total of 47 QTLs
were localized in the S42IL set, among which 39 QTLs were significant across all tested environments. For 19 QTLs (40.4%),
the wild barley introgression was associated with a favorable effect on trait performance. Von Korff et al. (2006 in Theor Appl Genet 112:1221–1231) mapped altogether 44 QTLs for six agronomic traits to genomic regions, which are represented
by wild barley introgressions of the S42IL set. Here, 18 QTLs (40.9%) revealed a favorable wild barley effect on the trait
performance. By means of the S42ILs, 20 out of the 44 QTLs (45.5%) and ten out of the 18 favorable effects (55.6%) were verified.
Most QTL effects were confirmed for the traits days until heading and plant height. For the six corresponding traits, a total
of 17 new QTLs were identified, where at six QTLs (35.3%) the exotic introgression caused an improved trait performance. In
addition, eight QTLs for the newly studied trait grains per ear were detected. Here, no QTL from wild barley exhibited a favorable
effect. The introgression line S42IL-107, which carries an introgression on chromosome 2H, 17–42 cM is an example for S42ILs
carrying several QTL effects simultaneously. This line exhibited improved performance across all tested environments for the
traits days until heading, plant height and thousand grain weight. The line can be directly used to transfer valuable Hsp alleles into modern elite cultivars, and, thus, for breeding of improved varieties. 相似文献
6.
Quantitative trait loci (QTLs) for plant height in rice were mapped onto the molecular marker linkage map of a double-haploid (DH) population derived from a cross between IR64 and Azucena under low and high N levels, in both nutrient solution and soil culture experiments. Two QTLs, one on chromosome 1 and the other on chromosome 8, were detected at high N levels in soil and/or nutrient solution culture experiments. A total of 8 QTLs were identified at low N level in the soil and/or nutrient solution culture experiments, which located on chromosomes 1, 2, 3, 4, 5 and 6, respectively. The QTL flanked by the molecular markers RZ730 RZ801 on chromosome 1 was identified in all experimental conditions. This position corresponds to sd-1, a semi-dwarfing gene. The QTLs on chromosomes 2, 3, 4, 5 and 6 were only detected at low N level and the QTL on chromosome 8 was only detected at high N level in the nutrient solution culture experiment. Based on the differing responses to low N stress between two parents and the QTL×N-level interaction observed in this study, it is suggested that the expression of several QTLs associated with plant height could be induced by low N stress. 相似文献
7.
von Korff M Wang H Léon J Pillen K 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(7):1221-1231
The objective of the present study was to identify favourable exotic Quantitative Trait Locus (QTL) alleles for the improvement
of agronomic traits in the BC2DH population S42 derived from a cross between the spring barley cultivar Scarlett and the wild barley accession ISR42-8 (Hordeum vulgare ssp. spontaneum). QTLs were detected as a marker main effect and/or a marker × environment interaction effect (M × E) in a three-factorial ANOVA. Using field data of up to eight environments and genotype data of 98 SSR loci, we detected 86
QTLs for nine agronomic traits. At 60 QTLs the marker main effect, at five QTLs the M × E interaction effect, and at 21 QTLs both the effects were significant. The majority of the M × E interaction effects were due to changes in magnitude and are, therefore, still valuable for marker assisted selection across
environments. The exotic alleles improved performance in 31 (36.0%) of 86 QTLs detected for agronomic traits. The exotic alleles
had favourable effects on all analysed quantitative traits. These favourable exotic alleles were detected, in particular on
the short arm of chromosome 2H and the long arm of chromosome 4H. The exotic allele on 4HL, for example, improved yield by
7.1%. Furthermore, the presence of the exotic allele on 2HS increased the yield component traits ears per m2 and thousand grain weight by 16.4% and 3.2%, respectively. The present study, hence, demonstrated that wild barley does harbour
valuable alleles, which can enrich the genetic basis of cultivated barley and improve quantitative agronomic traits. 相似文献
8.
Canci Paulo C. Nduulu Lexingtons M. Muehlbauer Gary J. Dill-Macky Ruth Rasmusson Donald C. Smith Kevin P. 《Molecular breeding : new strategies in plant improvement》2004,14(2):91-104
Validation of quantitative trait loci (QTLs) is a prerequisite to marker assisted selection (MAS), however, only a fraction of QTLs identified for important plant traits have been independently tested for validation. Resistance to the diseases kernel discoloration (KD) and Fusarium head blight (FHB) in barley is complex and technically difficult to assess, and therefore QTLs for these traits are suitable targets for MAS. We selected two lines, from a QTL mapping population created using the resistant variety Chevron, and crossed them to susceptible parents to generate two validation populations. Genetic maps of both populations were developed for five chromosomes covering 15 selected regions containing QTLs for FHB severity, KD score and concentration of deoxynivalenol (DON), a mycotoxin produced by the FHB pathogen. QTL analyses using these validation populations confirmed that five of the possible 15 QTL regions were associated with at least one of the three traits. While some QTL were detected inconsistently across environments, QTL that could be subjected to validation in both populations were confirmed in both populations in seven out of eight instances. A QTL for KD score on chromosome 6(6H) was confirmed in both validation populations in eight of nine environments and was also associated with FHB in three of six environments. A QTL for FHB on chromosome 2(2H) was confirmed and was also associated with KD and heading date. Marker assisted selection at these two QTLs should enhance disease resistance, however, the QTL on chromosome 2(2H) will also delay heading date. 相似文献
9.
Identification of RAPD markers linked to genetic factors controlling the milling energy requirement of barley 总被引:2,自引:0,他引:2
K. J. Chalmers U. M. Barua C. A. Hackett W. T. B. Thomas R. Waugh W. Powell 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1993,87(3):314-320
Doubled haploid (DH) populations of barley have been used in combination with PCR-based polymorphic-assay procedures to identify molecular markers linked to genes controlling the milling energy requirement of the grain. Milling energy (ME) is a quantitative trait and locating individual quantitative trait loci (QTLs) involved the construction of bulks by combining DNA from DH families representing the extreme members of the distribution for ME. In addition, the individuals had alternative alleles at theRrn2 locus that has previously been shown to be linked to an ME QTL. The DNA bulks were screened with Randomly Amplified Polymorphic DNA (RAPD) markers and polymorphic amplification products tested for linkage to genes influencing the expression of ME in a DH population. Several markers were identified which are linked to a QTL controlling ME and the recombination fraction determined by maximum likelihood procedures. The results indicate that DHs in combination with RAPDs and bulked segregant analysis provide an efficient method for locating QTLs in barely. Furthermore, this approach is applicable to mapping other QTLs in a range of organisms from which DH or recombinant inbred lines can be extracted. 相似文献
10.
Phenotype/genotype associations for yield and salt tolerance in a barley mapping population segregating for two dwarfing genes 总被引:3,自引:0,他引:3
Ellis RP Forster BP Gordon DC Handley LL Keith RP Lawrence P Meyer R Powell W Robinson D Scrimgeour CM Young G Thomas WT 《Journal of experimental botany》2002,53(371):1163-1176
Barley traits related to salt tolerance are mapped in a population segregating for a dwarfing gene associated with salt tolerance. Twelve quantitative trait loci (QTLs) were detected for seven seedling traits in doubled haploids from the spring barley cross Derkado x B83-12/21/5 when given saline treatment in hydroponics. The location of QTLs for seedling growth stage (leaf appearance rate), stem weight prior to elongation, and tiller number are reported for the first time. In addition, four QTLs were found for the mature plant traits grain nitrogen and plot yield. In total, seven QTLs are co-located with the dwarfing genes sdw1, on chromosome 3H, and ari-e.GP, on chromosome 5H, including seedling leaf response (SGa) to gibberellic acid (GA(3)). QTLs controlling the growth of leaves (GS2) on chromosomes 2H and 3H and emergence of tillers (TN2) and grain yield were independent of the dwarfing genes. Field trials were grown in eastern Scotland and England to estimate yield and grain composition. A genetic map was used to compare the positions of QTLs for seedling traits with the location of QTLs for the mature plant traits. The results are discussed in relation to the study of barley physiology and the location of genes for dwarf habit and responses to GA. 相似文献
11.
12.
Identification of QTLs for resistance to Fusarium head blight, DON accumulation and associated traits in the winter wheat variety Arina 总被引:3,自引:0,他引:3
Draeger R Gosman N Steed A Chandler E Thomsett M Srinivasachary Schondelmaier J Buerstmayr H Lemmens M Schmolke M Mesterhazy A Nicholson P 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(5):617-625
Fusarium head blight (FHB) of wheat has become a serious threat to wheat crops in numerous countries. In addition to loss
of yield and quality, this disease is of primary importance because of the contamination of grain with mycotoxins such as
deoxynivalenol (DON). The Swiss winter cultivar Arina possesses significant resistance to FHB. The objective of this study
was to map quantitative trait loci (QTL) for resistance to FHB, DON accumulation and associated traits in grain in a double
haploid (DH) population from a cross between Arina and the FHB susceptible UK variety Riband. FHB resistance was assessed
in five trials across different years and locations. Ten QTL for resistance to FHB or associated traits were detected across
the trials, with QTL derived from both parents. Very few of the QTL detected in this study were coincident with those reported
by authors of two other studies of FHB resistance in Arina. It is concluded that the FHB resistance of Arina, like that of
the other European winter wheat varieties studied to date, is conferred by several genes of moderate effect making it difficult
to exploit in marker-assisted selection breeding programmes. The most significant and stable QTL for FHB resistance was on
chromosome 4D and co-localised with the Rht–D1 locus for height. This association appears to be due to linkage of deleterious genes to the Rht-D1b (Rht2) semi-dwarfing allele rather than differences in height per se. This association may compromise efforts to enhance FHB resistance
in breeding programmes using germplasm containing this allele. 相似文献
13.
Detection and verification of malting quality QTLs using wild barley introgression lines 总被引:1,自引:0,他引:1
Inga Schmalenbach Klaus Pillen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(8):1411-1427
A malting quality quantitative trait locus (QTL) study was conducted using a set of 39 wild barley introgression lines (hereafter
abbreviated with S42ILs). Each S42IL harbors a single marker-defined chromosomal segment from the wild barley accession ‘ISR
42-8’ (Hordeum vulgare ssp. spontaneum) within the genetic background of the elite spring barley cultivar ‘Scarlett’ (Hordeum vulgare ssp. vulgare). The aim of the study was (1) to verify genetic effects previously identified in the advanced backcross population S42,
(2) to detect new QTLs, and (3) to identify S42ILs exhibiting multiple QTL effects. For this, grain samples from field tests
in three different environments were subjected to micro malting. Subsequently, a line × phenotype association study was performed
with the S42ILs in order to localize putative QTL effects. A QTL was accepted if the trait value of a particular S42IL was
significantly (P < 0.05) different from the recurrent parent as a control, either across all tested environments or in a particular environment.
For eight malting quality traits, altogether 40 QTLs were localized, among which 35 QTLs (87.5%) were stable across all environments.
Six QTLs (15.0%) revealed a trait improving wild barley effect. Out of 36 QTLs detected in a previous advanced backcross QTL
study with the parent BC2DH population S42, 18 QTLs (50.0%) could be verified with the S42IL set. For the quality parameters α-amylase activity and
Hartong 45°C, all QTLs assessed in population S42 were verified by S42ILs. In addition, eight new QTL effects and 17 QTLs
affecting two newly investigated traits were localized. Two QTL clusters harboring simultaneous effects on eight and six traits,
respectively, were mapped to chromosomes 1H and 4H. In future, fine-mapping of these QTL regions will be conducted in order
to shed further light on the genetic basis of the most interesting QTLs. 相似文献
14.
Agricultural environments deteriorate due to excess nitrogen application.Breeding for low nitrogen responsive genotypes can reduce soil nitrogen input.Rice genotypes respond variably to soil available nitrogen.The present study attempted quantification of genotype x nitrogen level interaction and mapping of quantitative trait loci (QTLs) associated with nitrogen use efficiency (NUE) and other associated agronomic traits.Twelve parameters were observed across a set of 82 double haploid (DH) lines derived from IR64/Azucena.Three nitrogen regimes namely,native (0 kg/ha; no nitrogen applied),optimum (100 kg/ha) and high (200 kg/ha) replicated thrice were the environments.The parents and DH lines were significantly varying for all traits under different nitrogen regimes.All traits except plant height recorded significant genotype x environment interaction.Individual plant yield was positively correlated with nitrogen use efficiency and nitrogen uptake.Sixteen QTLs were detected by composite interval mapping.Eleven QTLs showed significant QTL x environment interactions.On chromosome 3,seven QTLs were detected associated with nitrogen use,plant yield and associated traits.A QTL region between markers RZ678,RZ574 and RZ284 was associated with nitrogen use and yield.This chromosomal region was enriched with expressed gene sequences of known key nitrogen assimilation genes. 相似文献
15.
M. Muñoz-Amatriaín A. M. Castillo X. W. Chen L. Cistué M. P. Vallés 《Molecular breeding : new strategies in plant improvement》2008,22(1):119-129
In cereals, albinism is a major obstacle to produce doubled haploids (DH) for breeding programs. In order to identify QTLs
for green plant percentage in barley anther culture, a specific population was developed. This population, consisting of 100
DH lines, was generated by crossing the model cultivar for anther culture “Igri” with an albino-producing DH line (DH46) selected
from Igri × Dobla, in search of a maximum segregation for the trait and minimum for the other anther culture variables. A
combination of bulked segregant analysis and AFLP methodology was used to identify markers linked to the trait. A linkage
map was constructed using these AFLPs, together with RAPD, STS and SSR markers. This study identified a new QTL for green
plant percentage on chromosome 3H and confirmed the previously reported one on chromosome 5H. Up to 65.2% of the phenotypic
variance for this trait was explained by the additive effects of these two QTLs. Thirty elite cultivars of barley from different
origin, row type, growth habit and end use, were selected to validate these QTLs. Since two of the markers linked to the QTLs
were AFLPs, we successfully converted them into simple PCR-based SCAR markers. Only the SSR HVM60, on chromosome 3H, was significantly
associated with the trait, explaining near 20% of the phenotypic variance. Among the allelic variants identified for this
marker, HVM60-120bp was associated with the highest values of green plant percentage. 相似文献
16.
Hori K Sato K Takeda K 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(6):869-876
Seed dormancy is one of the most important traits in germination process to control malting and pre-harvest sprouting in barley
(Hordeum vulgare L.). EST based linkage maps were constructed on seven recombinant inbred (RI) and one doubled haploid (DH) populations derived
from crosses including eleven cultivated and one wild barley strains showing the wide range of seed dormancy levels. Seed
dormancy of each RI and DH line was estimated from the germination percentage at 5 and 10 weeks post-harvest after-ripening
periods in 2003 and 2005. Quantitative trait loci (QTLs) controlling seed dormancy were detected by the composite interval
mapping procedure on the RI and DH populations. A total of 38 QTLs clustered around 11 regions were identified on the barley
chromosomes except 2H among the eight populations. Several QTL regions detected in the present study were reported on similar
positions in the previous QTL studies. The QTL on at the centromeric region of long arm of chromosome 5H was identified in
all the RI and DH populations with the different degrees of dormancy depth and period. The responsible gene of the QTL might
possess a large allelic variation among the cross combinations, or can be multiple genes located on the same region. The various
loci and their different effects in dormancy found in the barley germplasm in the present study enable us to control the practical
level of seed dormancy in barley breeding programs.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
17.
Moritani M Togawa K Yaguchi H Fujita Y Yamaguchi Y Inoue H Kamatani N Itakura M 《Genomics》2006,88(6):719-730
To identify the disease-susceptibility genes of type 2 diabetes, we performed quantitative trait loci (QTL) analysis in F(2) populations generated from a BKS.Cg-m+/+Lepr(db) and C3H/HeJ intercross, taking advantage of genetically determined obesity and diabetes traits associated with the db gene. A genome-wide scan in the F(2) populations divided by sex and db genotypes identified 14 QTLs in total and 3 major QTLs on chromosome (Chr) 3 (LOD 5.78) for fat pad weight, Chr 15 (LOD 6.64) for body weight, and Chr 16 (LOD 8.15) for blood glucose concentrations. A linear-model-based genome scan using interactive covariates allowed us to consider sex- or sex-by db-specific effects of each locus. For the most significant QTL on Chr 16, the high-resolution haplotype comparison between BKS and C3H strains reduced the critical QTL interval from 20 to 4.6 Mb by excluding shared haplotype regions and identified 11 nonsynonymous single-nucleotide polymorphisms in six candidate genes. 相似文献
18.
Steven E. Ullrich Janet A. Clancy Isabel A. del Blanco Hyejin Lee Vadim A. Jitkov Feng Han Andris Kleinhofs Kunihiko Matsui 《Molecular breeding : new strategies in plant improvement》2008,21(2):249-259
Preharvest sprouting (PHS) can be a problem in barley (Hordeum vulgare L.) especially malting barley, since rapid, uniform, and complete germination are critical. Information has been gained by
studying the genetics of dormancy (measured as germination percentage, GP). The objective of this study was to determine if
the quantitative trait loci (QTLs) discovered in previous research on dormancy are related to PHS. PHS was measured as sprout
score (SSc) based on visual sprouting in mist chamber-treated spikes and as alpha-amylase activity (AA) in kernels taken from
mist chamber-treated spikes that showed little or no visible sprouting. GP was also measured. All traits were measured at
0 and 14 days after physiological maturity. Evaluation of the spring six-row cross, Steptoe (dormant)/Morex (non-dormant)
doubled haploid mapping population grown in greenhouse and field environments revealed QTL regions for SSc, AA, and GP on
five, four, and six of the seven barley chromosomes, respectively. In total, seven and eight regions on five and six chromosomes
had effects ranging from 4 to 31% and 3 to 39% on PHS and dormancy, respectively. One chromosome 3H and three chromosome 5H
QTLs had the greatest effects. All PHS QTLs coincide with known dormancy QTLs, but some QTLs appear to be more important for
PHS than for dormancy. Key QTLs identified should benefit breeding of barley for a suitable balance between PHS and dormancy. 相似文献
19.
S. Srinivasan S. Michael Gomez S. Satheesh Kumar S. K. Ganesh K. R. Biji A. Senthil R. Chandra Babu 《Plant Growth Regulation》2008,56(3):245-256
Drought stress is the major constraint to rice (Oryza sativa L.) production and yield stability in rainfed ecosystems. Identifying genomic regions contributing to drought resistance
will help to develop rice cultivars suitable for rainfed regions through marker-assisted breeding. Quantitative trait loci
(QTLs) linked to leaf epicuticular wax, physio-morphological and plant production traits under water stress and irrigated
conditions were mapped in a doubled haploid (DH) line population from the cross CT9993-5-10-1-M/IR62266-42-6-2. The DH lines
were subjected to water stress during anthesis. The DH lines showed significant variation for epicuticular wax (EW), physio-morphological
and plant production traits under stress and irrigated conditions. A total of 19 QTLs were identified for the various traits
under drought stress and irrigated conditions in the field, which individually explained 9.6%–65.6% of the phenotypic variation.
A region EM15_10-ME8_4-R1394A-G2132 on chromosome 8 was identified for leaf EW and rate of water loss i.e., time taken to
reach 70% RWC from excised leaves in rice lines subjected to drought stress. A large effect QTL (65.6%) was detected on chromosome
2 for harvest index under stress. QTLs identified for EW, rate of water loss from excised leaves and harvest index under stress
in this study co-located with QTLs linked to shoot and root-related drought resistance traits in these rice lines and might
be useful for rainfed rice improvement. 相似文献
20.
Magdalena Wójcik-Jagła Marcin Rapacz Mirosław Tyrka Janusz Kościelniak Katarzyna Crissy Katarzyna Żmuda 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(12):3021-3034