广东地区健康人肠道菌群调查分析

用直接快速涂片和缩小视野时钟法,检查粪便细菌分布,并与大便菌群培养定量计数比较,观察分析８２例不同年龄广东健康人群。结果表明：老年组（６０岁－７８岁）和儿童组（３岁－１０岁）较婴儿组（３月－１２月）的Ｇ＾－ｂ有明显增加（Ｐ＜０．０５）。男女性别无显著差异：Ｇ＾＋ｂ和Ｇ＾－ｂ的数量,随着年龄的增加而减少;Ｇ＾＋ｃ则随着年龄增加而增加。通过本次调查我们认为缩小视野时钟法,快速准确简便,容易掌握,价格低     

在共培养体系中单克隆抗体AH6对小鼠胚泡着床的影响

以单克隆抗体ＡＨ６「特异结合Ｌｅ＾Ｙ寡糖,Ｆｕｃａ１－２Ｇａｌβ１－４（Ｆ痉－３）ＧｌｃＮＡｃ－」以及其它３种与Ｌｅ＾Ｙ寡糖结构近似的寡糖特异结构的单克隆抗体ａＬｅ＾ａ、ａＬｅ＾ｂ和ＦＥ－Ａ５为工具,采用小鼠体外着床模型。研究细胞表面Ｌｅ＾Ｙ寡糖抗原与着床的关系及其在着床过程中具体作用环节。     

内皮素和bFGF对MC的促增殖作用及胰岛素的协同效应

采用３Ｈ－ＴｄＲ参入法,测定碱性成纤维细胞生长因子（ｂＦＧＦ）、胰岛素和内皮素－１（ＥＴ－１）对体外培养的大鼠肾小球系膜细胞（ＭＣ）增殖的影响,以及胰岛素与ｂＦＧＦ或ＥＴ－１促ＭＣ增殖的协同作用。结果表明,不同浓度的ｂＦＧＦ（５－２００ｎｇ／ｍｌ）和胰岛素（０．１－２．４Ｕ／ｍｌ）均显著升高ＭＣ的３Ｈ－ＴｄＲ参入值（ｃｐｍ值）（Ｐ＜０．０１）。ＥＴ－１对ＭＣ的ｃｐｍ值的影响依剂量不同呈现两种不同的效应,在１０－９－１０－７ｍｏｌ／Ｌ时,随着浓度的升高,ＭＣ的ｃｐｍ值明显升高（Ｐ＜０．０１）,并以１０－８ｍｏｌ／Ｌ作用最强;当升高到１０－６ｍｏｌ／Ｌ时,ＭＣ的ｃｐｍ值出现降低趋势。胰岛素与ｂＦＧＦ或低浓度ＥＴ－１（≤１０－８ｍｏｌ／Ｌ）共同作用于ＭＣ时,ＭＣ的ｃｐｍ值明显高于二者单独作用之和（Ｐ＜０．０１）,与高浓度ＥＴ－１（＞１０－７ｍｏｌ／Ｌ）共同作用于ＭＣ时,ＭＣ的ｃｐｍ值小于二者单独作用之和（Ｐ＞０．０５）。上述结果说明,胰岛素、ｂＦＧＦ和ＥＴ－１均能显著促进ＭＣ增殖;胰岛素与ｂＦＧＦ或低浓度的ＥＴ－１促ＭＣ增殖具有正协同作用,与高浓度ＥＴ－１呈现负协同作用。     

碱性成纤维细胞生长因子对大鼠肾小球系膜细胞原癌基因c-fos和c-myc表达的影响

在建立大鼠肾小球系膜细胞（ＭＣ）体外培养方法的基础上,通过３Ｈ－ＴｄＲ参入实验,ＲＮＡ印迹分析和斑点杂交观察ｂＦＧＦ对ＭＣＤＮＡ合成及原癌基因ｃ－ｆｏｓ和ｃ－ｍｙｃ表达的影响．结果表明,ｂＦＧＦ作用于ＭＣ１８ｈ,ＭＣ的３Ｈ－ＴｄＲ参入率明显增加（Ｐ＜０．０５）,２４ｈ达到高峰（Ｐ＜０．０１）;ｂＦＧＦ显著诱导原癌基因ｃ－ｆｏｓ和ｃ－ｍｙｃ表达,其表达活性分别于３０ｍｉｎ和１ｈ达到高峰．提示ｂＦＧＦ是ＭＣ的强效丝裂原,其对ＭＣＤＮＡ合成的促进作用与诱导原癌基因ｃ－ｆｏｓ和ｃ－ｍｙｃ表达有关．     

肉桂立木材积表的编制及优化

根据１４３株样木资料，选择材积方程Ｖ－ａＤ＾ｂ和Ｖ－ａＤ＾ｂＨ＾ｃ编制肉桂人工林一元、二元立木材积表，并用改进单纯形法进行优化，材积表经过检验，证明在生产上有实用价值。     

干旱胁迫对黄瓜PSⅡ颗粒铁组分Mossbauer谱的影响

黄瓜（Ｇｃｕｍｉｓ ｓａｔｉｖｕｓ Ｌ）叶片ＰＳⅡ颗粒的Ｍｏｓｓｂａｕｅｒ谱呈现４套双峰,依它们的化学位移相四敬矩劈塑数值,分别属于氧化态Ｃｙｔ－ｂ５５９,还原态Ｃｙｌ－ｂ５５９、Ｆｅ＾３＋－Ｑ画物和Ｆｅ＾２＋－Ｑ复合物。干埋胁迫旱影响ＱＡ／ＱＢ中铁（Ｆｅ）参与电子传递的速率,使ＰＳⅡ颗粒的ｏｓｓｂａｕｅｔ谱中Ｆｅ＾２＋的吸收双峰消失,即还原态Ｇ７ｙｔ－Ｂ５５９转变为氧化态Ｃｙｔ－ｂ５５９Ｆｅ＾２     

碱性成纤维细胞生长因子对大鼠肾小球系膜细胞原癌基因c—fos和c—…

在建立大鼠肾小球系膜细胞体外培养方法的基础上,通过＾３Ｈ－ＴｄＲ参入实验,ＲＮＡ印迹分析和斑点杂交观察ｂＦＧＦ对ＭＣ ＤＮＡ合成及原癌基因ｃ－ｆｏｓ和ｃ－ｍｙｃ表达的影响。     

Watkinson时滞差分方程模型的全局渐近稳定性

考虑差分方程ｘｎ＋１＝λｘｎ／（１＋ａｘｎ－ｋ）＾ｐ＋ｂλｘｎ－ｍ,ｎ＝０,１,２,…,其中ａ,ｂ,ｐ＞０,λ＞１,ｋ,ｍ∈｛０,１,２,…｝,当ｋ＝ｍ＝０时,Ｗａｔｋｉｎｓｏｎ用此方程来描述热带地区季蜀黍属作物的生长规律,当Ｐ＝１时,此方程就是著名的含多个滞量的Ｌｏｇｉｓｔｉｃ微分方程的离散模拟,本文主要目的是研究该方程唯一正平衡解的全局渐近稳定性。     

bcl-2癌基因蛋白在鼻咽癌以及单克隆癌细胞裸鼠体内连续传代中的表达

用免疫组织化学Ｓ－Ｐ方法,检测了４０例低分化鼻咽癌、３０例鼻咽癌克隆细胞裸鼠移植瘤、１０例慢性鼻咽炎及８例人胚鼻咽上皮组织石蜡包埋切片中抗凋亡基因ｂｃｌ－２癌蛋白的表达;并进一步检测了鼻咽癌克隆株在裸鼠体内演进中ｂｃｌ－２癌蛋白的表达以及与淋巴结转移之间的关系。结果表明：鼻咽癌及其裸鼠移植瘤组织中ｂｃｌ－２癌蛋白的表达率分别为６２．５％和７０％,慢性鼻咽炎中ｂｃｌ－２癌蛋白的表达率为１０％,人胚鼻咽上皮组织中不表达ｂｃｌ－２癌蛋白,鼻咽癌及其裸鼠移植瘤组织中ｂｃｌ－２癌蛋白的表达率明显高于慢性鼻咽炎组织中（Ｐ＜０．０１）;鼻咽癌克隆株演进中癌细胞ｂｃｌ－２癌蛋白的表达不断升高,其淋巴结转移率亦不断升高。提示：抗凋亡基因ｂｃｌ－２癌蛋白的表达可能和鼻咽癌的发生、演进及转移能力密切相关     

侧脑室注射血管紧张素Ⅱ对大鼠血压和缰核内神经元电活动的影响

实验观察了侧脑室注射（ｉｃｖ）及缰核（ｈａｂｅｎｕｌａ ｎｕｃｌｅｕｓ）内微电泳血管紧张素Ⅱ（ＡⅡ）与「Ｓａｒ＾１,Ｔｈｒ＾３」－ＡⅡ（ＳＴ－ＡⅡ,ＡⅡ拮抗剂）对正常和诮激性高血压（ｓｔｒｅｓｓ－ｉｎｄｕｃｅｄ ｈｙｐｅｒｔｅｎｓｉｏｎ ＳＩＨ）大鼠血压及内外侧缰核（ＭＨｂ、ＬＨｂ）内心血管神经元入电活动的影响。结果如下：ｉｃｖ ＡⅡ或ＳＴ－ＡⅡ,正常鼠和ＳＩＨ大鼠血压均升高或降低,ＳＩＨ鼠较正常     

The ridge-counts of the interdigital a-b,b-c,and c-d areas in a normal sample and in cerebrally damaged patients of Thuringia,G.D.R. (East Germany)

We determined the ridge-counts and their correlations of interdigital area a-b, b-c, and c-d among 300 boys and 300 girls from Jena and 95 male and 79 female cerebrally damaged chidren from the Mental Hospital of Stadroda, both in Thuringia, G.D.R. (East Germany). The mean values of the normals and the patients differ significantly only on the right hand of males in the b-c area (p ≤ 5%). Bimanuar comparisons showed significant correlations in the three areas in both sexes in the normal and the abnormal children. Inter-area comparisons revealed no significant correlation between a-b and b-c areas; correlations were significant between a-b and c-d areas in both sexes in the normal group, but significant for the right hand only in cerebrally damaged children. Highly significant correlation was found between the b-c and c-d areas only in the right hand of female cerebrally damaged children. Comparative data from Bavaria and North-Rhine-Westfalia are also presented.     

Prevalence of oral colonization with Candida albicans and anti-C. albicans IgA in the saliva of normal children and children with acute lymphoblastic leukaemia

The prevalence of oral colonization with C. albicans was studied in 40 children with acute lymphoblastic leukaemia (ALL) and 40 matched normal children. Colonization was more common in the ALL group (25% colonized) than the normal group (7.5% colonized). Total saliva IgA levels were lower in the ALL group, but low levels did not correlate with oral colonization. The three normal children with oral colonization all had anti-C. albicans IgA in their saliva, as had seven of the ten colonized ALL patients.     

Effect of different growth hormone dosages on the growth velocity in children born small for gestational age

To assess whether short-term growth hormone (GH) treatment can improve the linear growth in children who were born small for gestational age (SGA), we started a randomized multicenter trial in 26 age- and sex-matched prepubertal children born SGA. During the 1st year of GH therapy, all children received GH 0.23 mg/kg/week, then during the 2nd year, 13 children received the same dose (group A), and in the other 13 children, the dose of GH was doubled, i.e., 0.46 mg/kg/week (group B). During the 1st year of therapy, the growth velocity significantly (p<0.0001) increased in all patients. During the 2nd year, group A showed a significant decrease of the growth velocity (p<0.015), whereas group B maintained the growth rate. The height in group A children significantly increased during the 1st and the 2nd year of GH therapy (p<0.000002 and p<0.000001, respectively), reaching the normal range in 8 out of 13 children at the end of 2 years of GH therapy. The height in group B children significantly increased during the 1st and the 2nd year of GH therapy (p<0.000001 and p<0.000001, respectively), reaching the normal range in all 11 children who completed the GH therapy. The height gain was similar in groups A and B treated with the same GH dosage during the 1st year of therapy. A greater increase in height gain was found in children of group B treated with the higher GH dosage during the 2nd year of therapy as compared with group A (p<0.02). Significant increases in insulin-like growth factor I (p<0.0001), acid-labile subunit (p<0.0002), and bone/chronological age ratio (p<0.0001) were found after the 1st year of GH therapy, but no significant changes were observed during the 2nd year, independently of the GH dose. In conclusion, the height velocity of children born SGA significantly increases during the 1st year of GH therapy, diminishes, but can decrease during the 2nd year, if the GH dosage is not raised.     

Growth hormone normalises height, prediction of final height and hand length in children with Prader-Willi syndrome after 4 years of therapy

BACKGROUND: Based on the reported favourable effects of growth hormone (GH) treatment on growth and body composition in Prader-Labhart-Willi syndrome, we studied age dependency and the long-term effects on growth dynamics to elucidate the assumed hypothalamic GH deficiency. METHODS: We examined 23 children treated with hGH (24 U/m(2)/week) during a median of 4 (range 1.5-5.5) years; group 1: 10 young underweight (age 0.3-4.1 years), group 2: 8 prepubertal overweight (age 3.7-9.5 years) and group 3: 5 pubertal overweight children (age 9.0-14.6 years). RESULTS: After 4 years of therapy, height gain amounted to 1.8 SD; height (0.0 SD) and hand length (-0.2 SD) were normalised in the 2 prepubertal groups; in children above 6 years, height prediction approached parental target height. Weight for height rose in group 1 (to 0.64 SD) and decreased in group 2 (to 0.71 SD) to normal levels. Bone maturation of the pubertal children was too advanced to show a clear growth response to GH (height gain 0.42 SD). Even in this group, weight for height was reduced, but remained supernormal. CONCLUSION: Under exogenous GH, growth and body proportions are normalised in prepubertal children. With early institution of treatment, final height prediction reaches the parental target height range after 3 years. Such a growth-promoting effect of exogenous GH has so far only been described in children with GH deficiency.     

Lead,copper, zinc,and magnesium levels in hair of children and young people with some disorders of the osteomuscular articular system

The lead, copper, zinc, and magnesium contents of scalp hair taken from 173 children aged 1–15 yr and young people (16–18 yr) with certain disorders of the osteomuscular articular system (osteomuscular pains of unknown origin, once described as “growing pains”) were measured, using the flame atomic absorption spectrometry method, and then compared with those of 108 normal, healthy children. The research showed increased average levels of lead (a statistically significant p<0.05 in both the overall group of children, and in those over 11 yr old), and zinc (increased in the total group, in a statistically significant way at p<0.10 only in adolescents over 15 yr old) and decreased levels of copper (although not significantly) in the hair of children suffering from “rheumatic” diseases, as compared with controls. The magnesium levels for the total group of ill children were admittedly enhanced, but in the youngest children, the levels were reduced. The values of the Mg/Pb and Mg/Zn ratios were lower (in the youngest children, 70% decrease of the Mg/Pb ratio) and Zn/Cu were higher in the group of children suffering from rheumatic diseases than in the healthy children. The difference of Mg/Pb ratio between the total controls and rheumatic subjects was statistically significant at p<0.05 and the Zn/Cu at p<0.10. The Mg/Zn ratio was not statistically significant.     

运动疗法联合作业疗法用于脑瘫患儿的疗效及对肢体运动功能作用

摘要 目的：探讨运动疗法联合作业疗法用于脑瘫患儿的疗效对肢体运动功能的作用。方法：选择2020年3月~2022年6月我院康复科收治的80例脑瘫患儿作为研究对象,采用随机数字表法分为两组各40例,对照组予以运动疗法干预,包括Bobath技术和核心稳定性训练,试验组在对照组基础上进行作业疗法干预,包括手功能、手眼协调能力、进食、如厕、更衣及游戏训练。干预前后采用粗大运动功能测量（GMFM）、精细运动功能评估表（FMFM）及Gesell发育量表对患儿进行评估,评价临床疗效,并采用SF-36健康调查简表（SF-36）对患者进行评估。结果：两组患儿干预后的GMFM评分、FMFM评分均明显提高,而试验组明显高于对照组（P<0.05）。两组患儿干预后的DQ评分均明显提高,而试验组明显高于对照组,试验组DQ评分增幅明显大于对照组（P<0.05）。试验组的总有效率（95.0%）明显高于对照组（77.5%）（P<0.05）。两组患儿干预后SF-36评分均明显提高,试验组在活力、社会功能和精神健康维度明显高于对照组（P<0.05）。结论：运动疗法联合作业疗法可有效促进脑瘫患儿的上肢与下肢运动功能改善,提高康复效果,改善患儿生存质量,应充分重视脑瘫的综合康复疗法。     

Plasma zinc and copper in Paris area preschool children with growth impairment

Plasma zinc, copper, and parameters of growth were measured in a group of 116 French preschool children, 2–5 yr-old from low-income households. Participants were selected on the basis of Z-scores of weight for height (WHZ) and height for age (HAZ). Zinc and copper concentrations of children with growth impairment (GI), defined by a WHZ and/or HAZ< −1 Z-score, were compared to those of age, sex, and ethnic origin matched controls (WHZ and HAZ >−1 Z-score). Mean (±SD) plasma zinc concentration was 12.58±1.84 μmol/L in the GI group, and 13.27±1.98 μmol/L in the controls. The difference of the means of paired samples was 0.69±2.34, and by pairedt-test the significance reachedp=0.028. This effect was primarily a result of the weight retarded group (WHZ <−1 Z-score,p<0.009) and to the girls (p<0.05). There were no significant differences in plasma copper concentrations between groups. These results suggest the presence of marginal zinc deficiency in French preschool children with low weight for height Z-scores.     

赫哲族掌指纹特征研究

本文对166名健康赫哲族人的掌指纹进行了研究,其中男性86名,女性80名。本文列出了58个赫哲族掌指纹参数,将其中52个掌指纹特征输入电子计算机,进行了相关及主成份分析。并且,将赫哲族的掌指纹特征与汉族的掌指纹特征进行了比较。 结果表明:赫哲族的掌指纹特征是与汉族相似的,并且有自己的特征;双手同名指的相关系数大于非同名指,单手相邻指的相关系数大于相隔指;在有关掌指纹特征的研究中,指纹的重要性大于掌纹。     

Elevated Glutamatergic Compounds in Pregenual Anterior Cingulate in Pediatric Autism Spectrum Disorder Demonstrated by( 1)H MRS and (1)H MRSI

Recent research in autism spectrum disorder (ASD) has aroused interest in anterior cingulate cortex and in the neurometabolite glutamate. We report two studies of pregenual anterior cingulate cortex (pACC) in pediatric ASD. First, we acquired in vivo single-voxel proton magnetic resonance spectroscopy ((1)H MRS) in 8 children with ASD and 10 typically developing controls who were well matched for age, but with fewer males and higher IQ. In the ASD group in midline pACC, we found mean 17.7% elevation of glutamate + glutamine (Glx) (p<0.05) and 21.2% (p<0.001) decrement in creatine + phosphocreatine (Cr). We then performed a larger (26 subjects with ASD, 16 controls) follow-up study in samples now matched for age, gender, and IQ using proton magnetic resonance spectroscopic imaging ((1)H MRSI). Higher spatial resolution enabled bilateral pACC acquisition. Significant effects were restricted to right pACC where Glx (9.5%, p<0.05), Cr (6.7%, p<0.05), and N-acetyl-aspartate + N-acetyl-aspartyl-glutamate (10.2%, p<0.01) in the ASD sample were elevated above control. These two independent studies suggest hyperglutamatergia and other neurometabolic abnormalities in pACC in ASD, with possible right-lateralization. The hyperglutamatergic state may reflect an imbalance of excitation over inhibition in the brain as proposed in recent neurodevelopmental models of ASD.     

对比分析间歇正压通气与无创高频振荡通气分别联合微创肺表面活性物质治疗新生儿呼吸窘迫综合征疗效及安全性

摘要 目的：对比经鼻间歇正压通气（NIPPV）与无创高频振荡通气（nHFOV）分别联合微创肺表面活性物质（PS）治疗新生儿呼吸窘迫综合征（RDS）的临床效果及安全性。方法：选择2019年1月至2021年12月我院新生儿科收治的100例RDS患儿作为研究对象,采用随机数字表法分为对照组和观察组,各50例。对照组新生儿采用NIPPV联合微创PS治疗,观察组新生儿采用nHFOV联合微创PS治疗。比较两组患儿治疗相关指标（机械通气时间、氧暴露时间、住院天数）、临床症状（吸气三凹征、进行性呼吸困难、气促）改善时间、血气指标[脉血氧分压（PaO₂）、动脉血二氧化碳分压（PaCO₂）、氧合指数（OI）]、呼吸暂停发生率、通气失败率以及并发症发生率。结果：（1）观察组患儿机械通气时间、氧暴露时间、住院时间均较对照组少（P<0.05）。（2）观察组患儿进行性呼吸困难、吸气三凹征、气促改善时间均显著少于对照组（P<0.05）。（3）观察组患儿治疗72 h时的PaO₂显著高于对照组,PaCO₂和OI显著低于对照组（P<0.05）。（4）观察组呼吸暂停发生率和通气失败率为16.00%和10.00%,与对照组并无显著差异（P＞0.05）。（5）观察组术后并发症总发生率为4.00 %显著低于对照组的26.00 %（P<0.05）。结论：与NIPPV联合无创PS比较,nHFOV联合微创PS更能有效改善NRDS患儿肺通气功能,缩短机械通气时间,减少并发症。