共查询到19条相似文献,搜索用时 125 毫秒
1.
2.
表面增强拉曼散射(surface-enhanced Raman scattering,SERS)技术由于其灵敏度高、检测速度快、高特异性和无损等优点,在病原菌检测领域受到了广泛的关注。主要总结了近年来基于纳米信号标签的SERS方法在检测病原菌领域中的研究进展,并介绍了多功能SERS检测平台的构建及在病原菌检测中的应用。最后,对SERS这一技术作为实时、高效和可靠的病原菌检测工具的未来发展进行了展望。 相似文献
3.
4.
5.
6.
7.
董志伟 《中国生物工程杂志》1989,9(5):35-40
B淋巴细胞杂交瘤技术问世以来在肿瘤领域中已有广泛的应用。各种抗肿瘤单克隆抗体(MoAb)已用于肿瘤的血清学检测、组织病理分型、定位诊断及导向治疗。血清学检测的主要意义在于监视病情、判定预后及预报复发,在早诊方而迄今尚无突出进展。 相似文献
8.
9.
10.
11.
Robert E. Kuntz Betty June Myers Jerry A. Moore Tao-Cheng Huang 《International journal for parasitology》1975,5(1):21-26
Parasitological studies on 25 American opossums (Didelphis marsupialis) exposed to 1000 cercariae each of S. haematobium (Iran) have indicated a host-parasite situation and general parasitological conditions which show this marsupial to be a satisfactory host for experimental schistosomiasis. The return of schistosomes in relation to cercarial exposure was not excessively high, but there were egg deposits in the major visceral organs. Varied macroscopic pathology due to egg deposits in different organs was demonstrated as well as a potential for urinary bladder involvement, including tumor formation. Even though the precise nature of tumor pathology is not yet known, availability of the host, a good host-parasite compatability, and a low death rate in infected hosts favor this mammal as a model for experimental schistosomiasis haematobia. 相似文献
12.
In vivo tumor targeting and spectroscopic detection with surface-enhanced Raman nanoparticle tags 总被引:2,自引:0,他引:2
Qian X Peng XH Ansari DO Yin-Goen Q Chen GZ Shin DM Yang L Young AN Wang MD Nie S 《Nature biotechnology》2008,26(1):83-90
We describe biocompatible and nontoxic nanoparticles for in vivo tumor targeting and detection based on pegylated gold nanoparticles and surface-enhanced Raman scattering (SERS). Colloidal gold has been safely used to treat rheumatoid arthritis for 50 years, and has recently been found to amplify the efficiency of Raman scattering by 14-15 orders of magnitude. Here we show that large optical enhancements can be achieved under in vivo conditions for tumor detection in live animals. An important finding is that small-molecule Raman reporters such as organic dyes were not displaced but were stabilized by thiol-modified polyethylene glycols. These pegylated SERS nanoparticles were considerably brighter than semiconductor quantum dots with light emission in the near-infrared window. When conjugated to tumor-targeting ligands such as single-chain variable fragment (ScFv) antibodies, the conjugated nanoparticles were able to target tumor biomarkers such as epidermal growth factor receptors on human cancer cells and in xenograft tumor models. 相似文献
13.
Fluorescence is a mainstay of bioanalytical methods, offering sensitive and quantitative reporting, often in multiplexed or multiparameter assays. Perhaps the best example of the latter is flow cytometry, where instruments equipped with multiple lasers and detectors allow measurement of 15 or more different fluorophores simultaneously, but increases beyond this number are limited by the relatively broad emission spectra. Surface enhanced Raman scattering (SERS) from metal nanoparticles can produce signal intensities that rival fluorescence, but with narrower spectral features that allow a greater degree of multiplexing. We are developing nanoparticle SERS tags as well as Raman flow cytometers for multiparameter single cell analysis of suspension or adherent cells. SERS tags are based on plasmonically active nanoparticles (gold nanorods) whose plasmon resonance can be tuned to give optimal SERS signals at a desired excitation wavelength. Raman resonant compounds are adsorbed on the nanoparticles to confer a unique spectral fingerprint on each SERS tag, which are then encapsulated in a polymer coating for conjugation to antibodies or other targeting molecules. Raman flow cytometry employs a high resolution spectral flow cytometer capable of measuring the complete SERS spectra, as well as conventional flow cytometry measurements, from thousands of individual cells per minute. Automated spectral unmixing algorithms extract the contributions of each SERS tag from each cell to generate high content, multiparameter single cell population data. SERS-based cytometry is a powerful complement to conventional fluorescence-based cytometry. The narrow spectral features of the SERS signal enables more distinct probes to be measured in a smaller region of the optical spectrum with a single laser and detector, allowing for higher levels of multiplexing and multiparameter analysis. 相似文献
14.
Jie Zhang Simeng Chen Tianchen Gong Xiaolei Zhang Yong Zhu 《Plasmonics (Norwell, Mass.)》2016,11(3):743-751
A tapered optical fiber fabricated by a simple chemical etching method and modified with Ag nanoparticles (AgNPs) by chemical deposition was evaluated for surface-enhanced Raman scattering (SERS). The fiber probe was used for SERS measurements in both direct and remote scattering modes, yielding desired performance in both scattering configurations. The state of the obtained AgNPs made a significant contribution to the high sensitivity of SERS to Rhodamine 6G (R6G) molecules (down to a concentration of 10?7 M), and the substrate had an analyst enhancement factor (AEF) on the order of ~108. Meanwhile, the SERS intensity during the evaporation process was investigated, showing a good stability at the later stage of the evaporation process. The fiber SERS probes demonstrated good reproducibility with the average relative standard deviation (RSD) values being less than 0.2 for the major Raman peaks. 相似文献
15.
DNA methylation is a key diagnostic maker for genetic disease, cancer progression and pharmcogenomics. So far various techniques have been developed for DNA methylation assay, but most of them are laborious and time-consuming. Here we develop a simple and highly sensitive DNA methylation assay based on single base extension reaction and surface enhanced Raman spectroscopy (SERS). In the presence of methylated DNA, gold nanoparticle-modified capture probe can couple with a cyanine 5-deoxyribonucleoside triphosphate (cy5-dGTP) through single base extension reaction, and generates a high SERS signal after further addition of gold nanoparticles to increase the local electromagnetic field. While in the presence of unmethylated DNA, gold nanoparticle-modified capture probe cannot couple with cy5-dGTP due to the presence of a mismatch base, and no SERS signal is observed. This single base extension reaction-based SERS can determine methylated DNA with a detection limit of 3 pM, and can even distinguish as low as 1% methylation level in tumor suppressor gene CDKN2/p16/MTS1 (p16) from the mixtures. Notably, the sensitivity of this assay has improved by 5 orders of magnitude as compared to reported gold nanoparticle-based colorimetric assay, and by 2 orders of magnitude as compared to microarray-based methylation-sensitive single nucleotide primer extension assay (Ms-SNuPE). This method might be further applied to detect the methylation status in tumor-linked genes for cancer diagnosis. 相似文献
16.
Giuseppe Giallongo Roberto Pilot Christian Durante Gian Andrea Rizzi Raffaella Signorini Renato Bozio Armando Gennaro Gaetano Granozzi 《Plasmonics (Norwell, Mass.)》2011,6(4):725-733
Commercially available digital versatile discs (DVDs) contain a silver-coated spiral distribution of rectangular-shaped grooves
(AgDVD): for the first time, they have been used to produce surface-enhanced Raman scattering (SERS) substrates by electrochemical
deposition of silver nanoparticles (AgNPs@AgDVD). The overall procedure only requires cheap and widely available materials
and can be easily accomplished. Scanning electron microscopy images of AgNPs@AgDVD revealed that small AgNPs (average diameter
about 15 nm) are present within the valleys of AgDVD, whereas over the ridges, the AgNPs are bigger, more densely packed and
with a dendrite-like morphology somewhere. The SERS properties of these substrates have been studied in terms of the enhancement
factor (EF), point-to-point reproducibility and sample-to-sample repeatability. It turned out that high SERS EF and good reproducibility
requirements are both fulfilled. As for repeatability, remarkably better results than typical literature values have been
achieved. Such an easy&cheap preparation along with efficient SERS properties make DVD-derived SERS substrates very good candidates for the development
of convenient and disposable sensing platforms. 相似文献
17.
Ralph L. Brinster Howard Y. Chen Albee Messing Terry van Dyke Arnold J. Levine Richard D. Palmiter 《Cell》1984,37(2):367-379
A high percentage of transgenic mice developing from eggs microinjected with plasmids containing the SV40 early region genes and a metallothionein fusion gene develop tumors within the choroid plexus. A line of mice has been established in which nearly every affected animal succumbs to this brain tumor. Thymic hypertrophy and kidney pathology are also observed in some mice. SV40 T-antigen mRNA and protein are readily detected in affected tissues; however, SV40 T-antigen gene expression is barely detectable in unaffected tissues or in susceptible tissues prior to overt pathology, suggesting that tumorigenesis depends upon activation of the SV40 genes. Comparison of DNA from tumor tissue (or cell lines derived from tumors) with DNA from unaffected tissues reveals structural rearrangements as well as changes in DNA methylation of the foreign DNA. The SV40 genes are frequently amplified in tumor tissue, which further indicates that their expression is intimately involved in tumorigenesis in transgenic mice. 相似文献
18.
ChaoLing Du ChaoJun Du YuMeng You CongJun He Jian Luo DaNing Shi 《Plasmonics (Norwell, Mass.)》2012,7(3):475-478
We investigated the effect of optical thick metal films on the surface-enhanced Raman scattering (SERS) activity of individual Au nanoparticle (NP) monomers and dimers. The film presence is revealed to be positive for the SERS activity of individual NP monomers, while it is not always positive for the electromagnetic enhancement at hot spots for SERS of the dimer, which is explained well by our numerical simulations. The polarized SERS signals from the NP dimer are elucidated well in terms of the plasmon hybridization of the dimer. SERS contributions both from individual NP surfaces and the junction between the NP and its supporting substrate were discussed as well. 相似文献
19.
There is considerable interest in the development of novel platinum-based anticancer drugs that overcome the disadvantages associated with the widely used drug cisplatin, which are its inactivity against some types of tumors and its toxic side effects. In this study we show the suitability of normal Raman spectroscopy (NRS) and surface enhanced Raman spectroscopy (SERS), assisted by density functional theoretical (DFT) calculations, for the characterization of Pt complexes. The Pt complexes studied include the established drugs cisplatin and carboplatin, as well as five novel Pt complexes with anticancer activity. DFT calculations at the B3LYP/LanL2DZ level are a good prediction of the experimental NRS spectra of small and medium sized Pt complexes. The use of SERS allows the investigation of Pt complexes at physiological concentrations, and the binding strengths of the different ligands can be determined. The formation of positively charged hydrolysis products may be necessary for SERS activity. The exiting group in the hydrolysis reaction can be identified. 相似文献