滇重楼和毛重楼花粉活力和柱头可授性的研究

用TTC法测定了滇重楼和毛重楼花粉的活力,用联苯胺—过氧化氢法测定了其柱头的可授性。结果表明滇重楼的花粉活力在散粉后第1天活力较高以后则逐渐下降,在第2天和第19天花粉活力则急剧下降,但能维持20～23d,在整个花期中基本都具有活力。毛重楼的花粉寿命约为4d,第2天花粉活力达到最大值,第3天后花粉活力急剧下降,总体活力较低。滇重楼和毛重楼的柱头在整个花期中几乎都具可授性,但滇重楼在11～13d时柱头可授性最强,毛重楼在第5天可授性最强。两种重楼植物花粉活力较高的时期与柱头可授性较强的时期不一致,但柱头可授性较强的时间与分泌黏液较多的时间基本一致。

Pollen vitality and stigma receptivity of Paris polyphylla var.yunnanensis and P.mairei(Trilliaceae)

Pollen viability of Paris polyphylla var.yunnanensis and P.mairei was evaluated by TTC(2,3,5-triphenyl tetrazolium chloride)test,the stigma receptivity was estimated by benzidine-H2O2 method.The results were as follows:the pollen viability of P.polyphylla var.yunnanensis was the highest at the first day shedding pollens,then fell down gradually and lasted about 20 to 23 days.It could remain viable for nearly the entire flowering period but fell down suddenly in the second day and the 19th day.The pollen viability of P.mairei reached to a summit in the second day but fell down suddenly in the third day,the life-span was relatively short,only four days.The stigma of both species of Paris had receptivity during blooming period.It was the highest at 11 to 13 days after blooming in P.polyphylla var.yunnanensis but in P.mairei it was at the fifth day.There was a discrepancy in the peak time of pollen viability and stigma receptivity in both species of Paris.The summit of stigma receptivity and mucus secretion was nearly the same in two species.