| 纤维素酶分子结构和功能研究进展 |
| |
| 引用本文： | 阎伯旭,齐飞,张颖舒,高培基.纤维素酶分子结构和功能研究进展[J].生物化学与生物物理进展,1999,26(3):233-237. |
| |
| 作者姓名： | 阎伯旭 齐飞 张颖舒 高培基 |
| |
| 作者单位： | 山东大学微生物技术国家重点实验室,济南,250100 |
| |
| 基金项目： | 国家教委博士点基金资助项目. |
| |
| 摘 要： | 概述了近10年来利用结构生物学和蛋白质工程技术在纤维素酶分子结构和功能方面研究的进展，包括：酶分子结构域的拆分、催化域和纤维素结合结构域的结构和功能的研究，纤维素酶的分子折叠.并展望了该领域的研究前景.
|
| 关 键 词： | 纤维素酶，催化结构域，纤维素结合域结构域，分子折叠，活性位点，双置换机制 |
| 收稿时间： | 1998/2/19 0:00:00 |
| 修稿时间： | : 1998-02- |
Progress in Structure-Function Studies of Cellulases |
| |
| YAN Bo-Xu,QI Fei,ZHANG Ying-Shu and GAO Pei-Ji.Progress in Structure-Function Studies of Cellulases[J].Progress In Biochemistry and Biophysics,1999,26(3):233-237. |
| |
| Authors: | YAN Bo-Xu QI Fei ZHANG Ying-Shu and GAO Pei-Ji |
| |
| Institution: | State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China;State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China;State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China;State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China |
| |
| Abstract: | The recent advance in structure-function studies of cellulases by structural biology and protein engineering is reviewed.The seperation of structural domains and the structure-function studies of catalytic domains and cellulose-binding domains are included.The research prospects of cellulases are forecasted.According to the sequence similarity of amino acid, cellulase and hemicellulase can be divided in to nine families, and in five families there are some enzymes that three dimension structure have been resolved.Here, three dimension structure of some cellulase and xylanase were compared, and the relationship between its molecular folding and catalytic mechanism were also analyzed. |
| |
| Keywords: | cellulase catalytic domains cellulose-binding domains molecular folding active site two-step substitution mechanism |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
