| HCV 5′NCR转基因细胞HepG2.9706的特性分析 |
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| 引用本文： | 王小红,王升启,管伟,毛秉智.HCV 5′NCR转基因细胞HepG2.9706的特性分析[J].病毒学报,2002,18(1):74-76. |
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| 作者姓名： | 王小红 王升启 管伟 毛秉智 |
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| 作者单位： | 军事医学科学院,放射医学科学院,北京,100850 |
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| 基金项目： | 国家高技术研究发展计划(863计划);102-08-04-01; |
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| 摘 要： | 丙型肝炎是由丙型肝炎病毒(hepatitis C virus,HCV)引起的威胁人类健康的重要传染病,迄今尚无有效的疫苗及特异性抗病毒治疗药物.抗HCV药物的研究和开发面临的关键问题是缺乏合适的HCV感染细胞及动物评价模型.转基因细胞模型是研究人类疾病常用的有效手段,因此,在HCV分子生物学进展的基础上,我们以对HCV翻译与复制有明显调控功能的HCV 5′NCR及部分翻译起始区序列为靶标,构建了HCV 5′NCR及部分多聚蛋白起始区序列与荧光素酶基因的融合基因,插入真核表达载体,转染HepG2细胞,获得了HCV 5′NCR转基因细胞HepG2.9706细胞株1].本文对该细胞的某些特性进行了分析,以便更好地利用该细胞模型进行药物评价.
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| 关 键 词： | HepG2.9706细胞株 荧光定量PCR Southern杂交 拷贝数 荧光素 |
| 文章编号： | 1000-8721(2002)01-0074-03 |
The Biological Characteristics of HCV 5′NCR Transgenic Cell HepG2.9706 |
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| WANG Xiao hong,WANG Sheng qi,GUAN Wei,MAO Bing zhi.The Biological Characteristics of HCV 5′NCR Transgenic Cell HepG2.9706[J].Chinese Journal of Virology,2002,18(1):74-76. |
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| Authors: | WANG Xiao hong WANG Sheng qi GUAN Wei MAO Bing zhi |
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| Abstract: | In order to investigate the biological characteristics of the transgenic cellular model(HepG2.9706),the transgene copy numbers in HepG2.9706 were tested by complex probe fluorescent quantitative PCR and Southern hybridization.Its luciferase activity was assayed by luciferase assay system.The results showed that HepG2.9706,after consecutive passages for two years,keeps excellent luciferase activities and its transgene copy number is around 140 copies.This indicates that HepG2.9706 contains a high ratio of transgene integration and is very stable. |
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| Keywords: | HepG2 9706 fluorescent quantitative PCR Southern hybridization copy number luciferase |
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