桑粒肩天牛肠道纤维素分解细菌的分离和鉴定*

为研究天牛消化利用纤维素的机理，利用纤维素-刚果红琼脂培养基，从桑粒肩天牛(A.germari)幼虫中肠分离到一种兼性厌氧纤维素分解菌。菌落为白色圆形，边缘比较规则，周围溶纤维素透明圈直径一般可达10mm～20mm，细菌大小为0.5～0.8μm×1～3μm，革兰氏染色阳性，杆状，极生鞭毛，无芽孢。结合生化实验结果，初步鉴定该菌株为纤维单孢菌属(Cellulomonas)。

ISOLATION AND IDENTIFICATION OF THE CELLULOSE-UTILIZINGBACTERIA FROM THE GUT OF APRIONA GERMATI(HOPE)

In order to clarify the cellulose-utilizing mechanism of the Cerambycidae, the microorganism in the Apriona germari(Hope) larvae's gut was cultured and identified. A strain of facultative anaerobic cellulose-utilizing bacteria was isolated from the midgut fluid of A. germari larvae with the cellulose-congo red agar medium. The colonies of the bacteria were white and round with regular margin. Clear zones of cellulolysis surrounding the colonies might reach 10m～20mm. The size of the bacteria was 0.5μm～0. 8μm× l～3μm approximately. The cell was gram-positive rod with polar flagella. It hadn't spores. With the results of biochemical tests, the bacteria were tentatively identified as Cellulomonas.