| 小鼠动物实验方法系列专题(七)旷场实验在小鼠行为分析中的应用 |
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| 引用本文： | 王维刚,刘震泽,吴文婷,万颖寒,严惠敏.小鼠动物实验方法系列专题(七)旷场实验在小鼠行为分析中的应用[J].细胞生物学杂志,2011(11). |
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| 作者姓名： | 王维刚 刘震泽 吴文婷 万颖寒 严惠敏 |
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| 作者单位： | 同济大学化学系;同济大学生命科学与技术学院;上海南方模式生物研究中心; |
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| 基金项目： | 国家“863”计划(No.2008AA02Z126); 上海市科委科技创新行动计划(No.10140901400)资助项目~~ |
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| 摘 要： | 旷场实验是一个研究小鼠自发活动与探索行为的实验。该文以GAT1基因敲除小鼠为例介绍了旷场实验的原理和实验步骤。将小鼠置于箱型的旷场装置中,通过录像记录并分析小鼠在旷场中的活动。结果发现,GAT1基因敲除小鼠的自发活动与野生型小鼠无明显差异,而GAT1敲除小鼠的趋避性及焦虑水平较低。旷场实验简单、有效,是一项重要的行为学实验。
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| 关 键 词： | 旷场实验 行为学 γ-氨基丁酸转运蛋白1 自发活动 焦虑 |
The Use of Open Field Test in the Behavior Analysis of Mice |
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| Wang Weigang,Liu Zhenze,Wu Wenting,Wan Yinghan,Yan Huimin.The Use of Open Field Test in the Behavior Analysis of Mice[J].Chinese Journal of Cell Biology,2011(11). |
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| Authors: | Wang Weigang Liu Zhenze Wu Wenting Wan Yinghan Yan Huimin |
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| Institution: | Wang Weigang~(1,2),Liu Zhenze~2,Wu Wenting~3,Wan Yinghan~3,Yan Huimin~(3*) (1 Department of Chemistry,Tongji University,Shanghai 200092,China,2 School of Life Science and Technology,3 Shanghai Research Center for Model Organisms,Shanghai 201203,China) |
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| Abstract: | The open field test(OFT) is a simple and reliable behavior test for rodents.Here we used it for the behavior analysis of GAT1 mutant mice to illustrate its basic principle and protocol.Each mouse was put into the open field and its behaviors were recorded for further analysis.We found that GAT1 knockout mice do not show any abnormalities in their locomotive activities,but they manifest lower levels of depression and anxietyrelated behaviors compared to their wild type littermates.These results suggest that ... |
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| Keywords: | the open field test ethology GABA transporter 1 locomotion anxiety |
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