排序方式: 共有18条查询结果,搜索用时 15 毫秒
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用DSS喂饲小鼠建立小鼠IBD模型,在建模的第0、8、13天分别用碘帕醇灌胃加腹腔注射的方法造影并micro-CT扫描降结肠壁厚度,结果第8天和第13天分别为0.4586±0.04 mm和0.40325±0.03 mm,显著(P<0.001)大于对照组((0.28±0.02 mm).同时检测小鼠体重变化、临床评分、结肠... 相似文献
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去甲斑蝥素与单克隆抗体偶联物治疗肝癌实验研究 总被引:3,自引:0,他引:3
用大鼠单克隆抗体3A5与去甲斑蝥素(NCTD)制备偶联物,ELISA检测结果表明:3A5-NCTD偶联物保持对人肝癌BEL-7402细胞和小鼠肝癌H22细胞的免疫反应性。克隆生成法测定结果显示:3A5-NCTD具有比NCTD更强的细胞毒性。小鼠腹腔内移植肿瘤,ip给药,3A5-NCTD和NCTD的ILS值分别为111%和37%。结果说明在腹腔肿瘤的治疗方面3A5-NCTD具有比游离NCTD更强的治 相似文献
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感觉运动门控功能异常与多种精神疾病相关,前脉冲抑制是常用的测量感觉运动门控的重要行为学参数。该文介绍了用震惊反射系统测定C57BL/6J品系小鼠前脉冲抑制的实验方法,并用N-甲基-D-天冬氨酸受体非竞争拮抗剂MK-801(Dizocilpine,地卓西平)成功构建了药物诱发前脉冲抑制缺失的小鼠模型。 相似文献
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It was documented that type IV collagenase with two subtypes of 72 ku/MMP-2 and 92 ku/MMP-9 plays an important role in tumor invasion and metastasis. The endoplasmic reticulum (ER)- retained, single chain Fv antibody fragment (scFv) was used to inhibit the function of type IV collagenase. For expression in mammalian cells, the assembled scFv M97 gene with ER retention signal encoding 6 additional amino acids (SEKDEL) was reamplified by PCR. The amplified fragments were cloned into the pcDNA3.1 vector. The resulting plasmid was sequenced and then introduced into PG cells, a highly metastatic human lung cancer cell line, by lipofectAMINE method. The result of intrabody gene therapy showed that type IV collegenase expression was down regulated significantly as measured by ELISA. The biological behavior of PG cell, such as the ability of in vitro invasion through Matrigel, colony formation on soft agar, was also inhibited by scFv M97 transfection. Animal experiments in a xenograft model of human lung cancer 相似文献
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