中国对虾抗WSSV及其它经济性状的QTL定位初步研究

以中国对虾抗WSSV选育群体第四代雌虾和野生中国对虾雄虾为亲本，采用人工精荚移植方式产生F1代家系，家系内个体姊妹交获得R家系材料，42尾R家系个体采用口饲法进行WSSV（White Spot Syndrome Virus）攻毒实验，获得个体抗WSSV及其它相关数据。构建了中国对虾的AFLP（Amphfied Fragment Length Polymorphism）分子标记遗传连锁图谱。利用MAPMAKER／QTL1．1软件进行了中国对虾体长、全长、体重及抗WSSV性状的QTL（Quantitative TraitsLoci）定位分析，首次实现了中国对虾重要经济性状的QTL定位。在LOD值大于2．0的条件下，共检测到和体长相关的QTL位点1个，与全长相关的QTL位点2个，与体重相关的QTL位点2个，与抗WSSV性状相关的位点2个，分别位于3个连锁群上，位点变异解释率从26．6％-66．9％不等。在其中的1个连锁群上检测到了体重、全长和抗WSSV性状相关的三个QTL位点，1个连锁群上检测到了体重和抗WSSV性状相关的两个QTL位点，1个连锁群上检测到了全长和体长相关的两个QTL位点。表明在中国对虾在此生长阶段，抗WSSV性状和个体大小存在一定程度的正相关关系动物学报54（6）：1075-1081，2008]。

QTL associated with WSSV-resistance and growth-related traits in the Chinese shrimp Fenneropenaeus chinensis

A virgin female Chinese shrimp Fenneropenaeus chinensis was selected from the fourth generation of a successively WSSV(White Spot Syndrome Virus)resistance population and mated with a wild male through artificial insemination to produce the F_1 and F_2 generation.These shrimp were challenged with WSSV and the F_2 generation were collected and scored for WSSV resistance,standard length,total body length and body weight.AFLP(Amplified Fragment Length Polymorphism)markers were used to construct a linkage map o...