斑马鱼charon基因的克隆、抗体制备及分析

斑马鱼charon基因位于1号染色体上,其成熟肽编码区含有731 bp,编码243个氨基酸.charon基因编码Cerberus/Dan家族的一种分泌因子,它与心脏的左右不对称发育有关.为进一步研究charon在心脏左右不对称发育中的功能,以斑马鱼为动物模型,利用RT-PCR的方法成功克隆了斑马鱼charon基因片段,...     

卡龙游仆虫无性生殖期间的形态发生学研究：原生动物,纤毛虫

卡龙游仆虫为海洋中自由生纤毛虫,利用银染法对该种二分裂期间的形态发生学进行了初步的研究,其主要过程为：１．伴随大核改组带的出现和ＤＮＡ复制开始,口原基发生于老口围 方皮膜下一龛腔内,后由前至后组装成围口小膜而演化为后ＡＺＭ。老口围带及口侧膜在原位被被前仔虫继承;２．体棘毛场首先出现两组棘毛原基,其随后各自独立演化成９根前、后仔虫的额－腹－横棘毛;３．缘棘毛原基也为独立发生,初为单一,后断裂为二并分     

Geminin is required for left-right patterning through regulating Kupffer's vesicle formation and ciliogenesis in zebrafish

Geminin plays an important role in coordinating the cell cycle with anterior–posterior patterning during embryonic development. However, whether it is involved in the regulation of left–right (LR) patterning remains unknown. Here, we reported that geminin is required for setting up heart and visceral laterality during zebrafish development. Defective heart and visceral laterality was observed in geminin morphants. Further study demonstrated that the left-sided nodal/spaw in the lateral plate mesoderm (LPM) as well as the sideness of its downstream targets lefty2 and lefty1 was perturbed in geminin morphants. Upstream of the left-sided Nodal signal along the regulatory cascade of LR asymmetry, knock down of geminin resulted in defective Kupffer’s vesicle (KV) formation and ciliogenesis rather than middle line defects. Predominant distribution of an antisense morpholino against geminin in dorsal forerunner cells (DFCs) led to defective KV morphogenesis and perturbed LR asymmetry, similar to those of geminin morphants, indicating a cell-autonomous role of geminin in regulating KV formation and ciliogenesis. Our results demonstrate that geminin is required for proper KV formation and ciliogenesis, thus playing an important part in setting up LR asymmetry.