| 斑马鱼charon基因的克隆、抗体制备及分析 |
| |
| 引用本文： | 吴坚,王玲,戴悦,吴秀山,王跃群.斑马鱼charon基因的克隆、抗体制备及分析[J].激光生物学报,2011,20(3):347-352. |
| |
| 作者姓名： | 吴坚 王玲 戴悦 吴秀山 王跃群 |
| |
| 作者单位： | 湖南师范大学蛋白质化学及鱼类发育生物学教育部重点实验室,心脏发育研究中心,湖南长沙410081 |
| |
| 基金项目： | 国家自然科学基金项目，国家重点基础研究发展计划资助项目 |
| |
| 摘 要： | 斑马鱼charon基因位于1号染色体上,其成熟肽编码区含有731 bp,编码243个氨基酸.charon基因编码Cerberus/Dan家族的一种分泌因子,它与心脏的左右不对称发育有关.为进一步研究charon在心脏左右不对称发育中的功能,以斑马鱼为动物模型,利用RT-PCR的方法成功克隆了斑马鱼charon基因片段,...
|
| 关 键 词： | charon 融合蛋白 斑马鱼 原核表达 多克隆抗体 |
Cloning and Polyclonal Antibody Preparation of charon Gene in Zebrafish |
| |
| WU Jian,WANG Ling,DAI Yue,WU Xiu-shan,WANG Yue-qun.Cloning and Polyclonal Antibody Preparation of charon Gene in Zebrafish[J].ACTA Laser Biology Sinica,2011,20(3):347-352. |
| |
| Authors: | WU Jian WANG Ling DAI Yue WU Xiu-shan WANG Yue-qun |
| |
| Institution: | WU Jian,WANG Ling,DAI Yue,WU Xiu-shan~*,WANG Yue-qun (The Center for Heart Development,Key Lab of MOE for Development Biology and Protein Chemistry,Hunan Normal University,Changsha 410081,Hunan,China) |
| |
| Abstract: | The charon gene in zebrafish is located on chromosome 1,which coding region has 731 bp and encodes 243 amino acids.Charon encodes a Cerberus/Dan family secreted protein,which is related in left/right asymmetric heart development.Zebrafish gene charon has homologous genes in humans,mice and rats.In order to study the function of charon in left/right asymmetric heart development,a fragment of zebrafish gene charon was cloned by RT-PCR technique,then induced to express charon fushion protein with prokaryotic e... |
| |
| Keywords: | charon |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
