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Schisandra sphenanthera (Schisandraceae) is a traditional Chinese medicinal plant. In this study, nine polymorphic microsatellite loci were developed
and characterized for the species. A genomic DNA enrichment protocol was used to isolate microsatellite loci and polymorphism
was explored using 36 individuals sampled from a natural population. The observed number of alleles ranged from 2 to 10 with
an average of 5.2. Expected and observed heterozygosity ranged from 0.30 to 0.82 and 0.33 to 0.97, respectively. These microsatellites
have been directly applied to both population and conservation genetic studies of S. sphenanthera. 相似文献
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Peiying Shi Qing He Yufeng Zhang Haibin Qu Yiyu Cheng 《Phytochemical analysis : PCA》2009,20(3):197-206
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不同产地华中五味子叶表皮结构和导管分子的解剖学特征及其与环境因子的关系 总被引:20,自引:2,他引:18
在光学显微镜下观察了不同产地华中五味子(Schisandra sphenanthera Rehd.et Wils.)叶表皮结构和导管分子特征,应用多元回归方法对不同产地华中五味子的叶表皮特征及导管分子特征与环境因子的关系进行了分析。观察的指标有叶表皮特征(气孔器密度、气孔器指数、气孔器长宽、气孔器面积、气孔极区角质加厚、气孔器类型、表皮细胞垂周壁形状、分泌细胞密度及叶表面角质条纹)和导管分子特征(导管分子长度、直径、长/直径比值及穿孔板类型)。观察结果:上表皮有极少量气孔器和分泌细胞分布。在下表皮,气孔器类型为平列型和侧列型,气孔器指数为15.05%-22.53%,气孔器长59.2—74.2μm,气孔器宽37.9—46.8μm,分泌细胞密度为1.45/mm^2-3.99/mm^2。导管分子以具缘纹孔导管为主,穿孔板包含单穿孔和梯状穿孔板。导管分子长957.8—1270.2μm,导管分子直径85.0—136.7μm。随着产地的不同,华中五味子叶下表皮结构和导管分子的解剖特征有明显的差异。分析结果显示:年均气温与气孔器面积和气孔器长度,年降水量与气孔器宽度以及相对湿度与分泌细胞密度之间关系密切。随着年平均气温的升高,气孔器面积、气孔器长度呈减少的趋势,R^2值分别为0.74、0.71。随着年降水量的增加,气孔器宽呈减小的趋势,R^2值为0.64;导管分子长/直径比值增加,R^2值为0.46。相对湿度与分泌细胞密度呈正相关,R^2为0.63。 相似文献
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Li-Jia Xu Feng Huang Si-Bao Chen Lian-Niang Li Shi-Lin Chen Pei-Gen Xiao 《植物学报(英文版)》2006,48(12):1493-1497
In the course of our study of bioactive natural products from Schisandra plants, we isolated a neolignan from an EtOAc extract of the stems of Schisandra propinqua (Wall.) Baill. The structure of the new com- pound was determined to be 4, 4-di (4-hydroxy-3-methoxyphenly)-2, 3-dimethylbutanol (compound 1) on the basis of 1H- and 13C-NMR spectra and 2D NMR methods. Eight known compounds, compounds 2-9, were also isolated and identified, of which compounds 3, 4, 6 and 9 were isolated for the first time from this plant. In addition, compounds 1-4 were evaluated for cytotoxicity by an 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H- tetrazolium bromide (MTT) assay. Compound 1 showed significant potential cytotoxic ability in the bioassay. 相似文献
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华中五味子抗氧化和细胞毒活性研究 总被引:6,自引:0,他引:6
我们研究了华中五味子的根、茎、叶、果实乙醇提取物对脂性自由基DPPH及超氧阴离子的清除作用,同时观察了四种不同提取物在体外对肿瘤细胞株的细胞毒作用。我们发现,华中五味子根的乙醇提取物清除自由基活性和对肿瘤细胞杀伤作用比茎、叶和果实的提取物都强,研究结果提示华中五味子的根可以作为抗氧化剂的新来源。 相似文献
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《Archives of animal nutrition》2013,67(1):66-74
The study was conducted to evaluate the effects of dietary supplementation with different levels of two extracts, an aqueous extract of Ligustrum lucidum (AELL), and an ethanol extract of Schisandra chinensis (EESC) on growth performance, parameters of antioxidative status and spleen lymphocyte proliferation of broilers, respectively. The results showed that neither AELL nor EESC had significant effects on growth performance of broilers. However, malondialdehyde concentration in heart and liver of the broilers were significantly decreased by feeding AELL or EESC. Superoxide dismutase activity in heart, liver, and kidney of broilers were improved by feeding different dosages of AELL or EESC. In contrast, glutathione reductase activity in serum, heart and kidney of broilers was not affected by experimental treatment. In addition, spleen lymphocyte proliferation of broilers was significantly enhanced by feeding different dosages of AELL or EESC. In conclusion, the results suggested that either AELL or EESC may improve antioxidant status and immune function of broilers. 相似文献
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华中五味子干燥材料DNA提取方法的研究 总被引:3,自引:2,他引:1
目的:建立一套适合从华中五味子硅胶干燥的幼芽中提取高质量DNA的方法,为进一步开展分子生物学研究奠定基础。方法:比较SDS法、SIX-CTAB法、SDS-CTAB法以及经过作者改进的CTAB法,运用紫外光谱和电泳分析方法测定所得DNA样品的纯度与完整性。结果:四种方法中改进CTAB法所得DNA纯度最高,完整性好,其A260/A280值为1.81,A260/A230值为2.13,经稳定性试验证明该方法稳定。结论:改进CTAB法是一套适合从华中五味子硅胶干燥的幼芽中提取高质量DNA的方法。 相似文献
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