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A cDNA of 417 bp encoding an S-RNase gene, named PA S3, was isolated from apricot, Prunus aremeniaca. Nine S-alleles, S1–S9, were recognized by S-allele-specific PCR and confirmed by Southern blot analysis using PA S3 as probe. The S-genotypes of the six cultivars were determined and the results of self- and cross-pollination tests among the six cultivars were consistent with the predicted S-haplotypes by PCR analysis. 相似文献
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Hoy Taek Kim Gen Hattori Yutaka Hirata Dae Ill Kim Jeong Hwan Hwang Yong Uk Shin Ill Sup Nou 《Journal of Plant Biology》2006,49(6):448-454
To prevent self-fertilization, apple has a gametophytic self-incompatibility mechanism, part of a widespread intraspecific
system, that is controlled by a multi-allelic locus. This attribute has been exploited in breeding programs for new cultivars.
Likewise, many apple orchards depend on artificial pollination. Therefore, molecular analysis and early identification of
the self-incompatibility (S) genotype could greatly improve breeding schemes and pollen donors selection. Here, we PCR-amplified
the S-RNase PCR fragments from a total of 14 cultivars and parents, using new primers (ASPF3+ASPR3) common to 23 S-alleles
in apple. The S-genotypes were determined for the following: ‘Hongro’ (S1S3), ‘Gamhong’ (S1S9), ‘Saenara’ (S1S3), ‘Chukwang’ (S3S9), ‘Hwahong’ (S3S9), ‘Seokwang’ (S3S3), ‘Hwarang’ (S1S9), ‘Sunhong’ (S3S9), ‘S.E.B.’ (S1S19), ‘S.G.D.’ (S2S3), and ‘Mollie’s Delicious’ (S3S7). We also confirmed the characteristics of the S-genotypes for eight Korean apple cultivars by PCR-Southern blot analysis,
using seven S-RNases as probes. 相似文献
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Apple exhibits gametophytic self-incompatibility (GSI) that is controlled by the multiallelic S-locus. This S-locus encodes
polymorphicS ribonuclease (S-RNase) for the pistil-part 5 determinant. Information aboutS-genotypes is important when selecting pollen donors for fruit production and breeding of new cultivars. We determined the
5-genotypes of ‘Charden’ (S2S3S4), ‘Winesap’ (S1S28), ‘York Imperial’ (S2S31), ‘Stark Earliblaze’ (S1S28), and ‘Burgundy’ (S20S32), byS-RNase sequencing and S-allele-specific PCR analysis. Two newS-RNases, S31 and S32, were also identified from ‘York Imperial’ and ‘Burgundy’, respectively. These newS-alleles contained the conserved eight cysteine residues and two histidine residues essential for RNase activity. Whereas
S31 showed high similarity to S20 (94%), S32 exhibited 58% (to S24) to 76% (to S25) similarity in the exon regions. We designed newS-allele-specific primers for amplifying S31- and S32-RNasc-specific fragments; these can serve as specific gene markers. We also rearranged the apple S-allele numbers containing
those newS-RNases. They should be useful, along with anS-RNase-based PCR system, in determining S-genotypes and analyzing new alleles from apple cultivars. 相似文献
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基于cDNA芯片的梨品种S基因型鉴定及新S-RNase基因进化分析 总被引:1,自引:0,他引:1
梨品种S基因型鉴定对梨栽培中授粉品种选择和遗传育种都具有重要意义。本研究利用梨S-RNase基因荧光标记的特异引物PCR扩增获得梨品种荧光标记的cDNA特异产物;进一步完善梨S-RNase基因cDNA芯片,以被检测梨品种cDNA特异序列与梨S-RNase基因cDNA芯片杂交检测不同梨品种S基因型,并发现新的S-RNase基因。结果表明:利用梨S-RNase基因cDNA芯片鉴定了泸定王皮梨、兴山24号、弥渡百合等35个未知S基因型梨品种,确定了各品种的S基因型。结合PCRRFLP及DNA克隆和测序等技术,发现了7个新的S-RNase基因资源,获得了新S-RNase基因序列。序列分析表明各新S-RNase基因均具有S-RNase基因特异区域序列的典型特征;进化分析显示7个新S-RNase基因主要属于蔷薇科苹果亚科S-RNase类群,且存在种间和属间比种内和属内进化关系更近的现象。7个新的S基因分别命名为:PpS_(53)(Pyrus pyrifolia S53)、PpS_(54)、PpS_(55)、PpS_(56)、PpS_(57)、PpS_(58)和PpS_(59),GenBank登录号分别为:KX581753、KX581754、KX581755、KX581756、KX581757、KX581751和KX581752。 相似文献
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梨是配子体型自交不亲和植物,确定不同品种的S基因型是科学杂交授粉及提高梨产量和品质的基础。本文根据砂梨S1-9等位基因一级结构特征,设计特异引物PF和PR,以白梨(Pyrus bretschneideri)种鹅梨(Pyrus bretschneideri‘Eli’)和砂梨(Pyrus pyrifolia)品种博多青(Pyrus pyrifolia‘Hakataao’)的叶片基因组DNA为模板,通过PCR·RFLP系统检测、克隆测序以及生物信息学分析,分离鉴定了它们的片段大小相似的2条S等位基因,从中获得1条新的S基因,命名为S34-RNase基因,并确定了这2个梨品种的S基因型,分别为鹅梨S13S34和博多青S22S34。 相似文献
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H. Yaegaki T. Shimada T. Moriguchi H. Hayama T. Haji M. Yamaguchi 《Sexual plant reproduction》2001,13(5):251-257
Three partial S-RNase genes, MSRN-1, MSRN-2, and MSRN-3, in the Japanese apricot (Prunus
mume Sieb. et Zucc.) were isolated from the three cultivars Nankou, Gyokuei, and Kairyouuchidaume, respectively. The structural
characteristics revealed that S-RNase genes from the Japanese apricot were in the T2/SRNase-type S-RNase family with five conserved regions (C1, C2, C3, RC4, and C5) and one variable region (RHV) as reported in the other
rosaceous plants. In the phylogenetic tree of T2/S SRNase-type RNases, three S-RNase genes of the Japanese apricot did not form a species-specific subgroup but the Prunus subfamily did. At least seven S-allelic genes were present in the Japanese apricot, and S-genotypes of six representative cultivars, including Nankou, Gyokuei, Kairyouuchidaume, Baigou, Kagajizou, and Oushuku were
first established in this study as S
1
S
7, S
2
S
6, S
3
S
4, S
3
S
6, S
3
S
6 and S
1
S
5, respectively. An extended elucidation of the S-genotype would contribute to a more efficient breeding program of the Japanese apricot.
Received: 5 September 2000 / Revision accepted: 22 December 2000 相似文献
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