Identification of Self-Incompatibility Genotypes of Apricot (<Emphasis Type="Italic">Prunus armeniaca</Emphasis> L.) by S-Allele-Specific PCR Analysis |
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| Authors: | Qi?Jie Gai?Shupeng Zhang?Jixiang Gu?Manru Email author" target="_blank">Shu?HuairuiEmail author |
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| Institution: | (1) College of Horticulture, Shandong Agriculture University, 271018 Taian, China;(2) College of Marine Life Science, Ocean University of China, 266003 Qingdao, China;(3) Laiyang Agriculture College, 265200 Laiyang, China |
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| Abstract: | A cDNA of 417 bp encoding an S-RNase gene, named PA S3, was isolated from apricot, Prunus aremeniaca. Nine S-alleles, S1–S9, were recognized by S-allele-specific PCR and confirmed by Southern blot analysis using PA S3 as probe. The S-genotypes of the six cultivars were determined and the results of self- and cross-pollination tests among the six cultivars were consistent with the predicted S-haplotypes by PCR analysis. |
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| Keywords: | apricot cDNA S-genotype self-incompatibility S-RNase |
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