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排序方式: 共有233条查询结果,搜索用时 15 毫秒
1.
J M Mato 《Biochemical and biophysical research communications》1979,88(2):569-574
Human leucocyte cell cultures were stimulated to initiate DNA synthesis by phytohemagglutinin and mercuric chloride. Both mitogens enhanced the accumulation of β2-microglobulin in the medium, which was synthesized by lymphocytes. Mercuric chloride promoted the accumulation of this protein optimaly with a concentration (1 × 10?5M) to produce the maximum stimulation of DNA synthesis. Combined use of phytohemagglutinin (50 μg/ml) and mercuric chloride (1 × 10?5M) produced additive effect on both DNA synthesis and β2-microglobulin accumulation. These findings suggest that mercuric ion causes the proliferative response of lymphocytes by a mechanism different from that for the stimulation by phytohemagglutinin. 相似文献
2.
Pushpendra Singh Zoltan Beck Gary R. Matyas Carl R. Alving 《Journal of liposome research》2013,23(3):247-250
AbstractLiposomes containing cholesterol and monophosphoryl lipid A (such as ALFQ and AS01B) are vaccine adjuvants. During construction of the formulations, addition of QS21 to nano-size (50–100?nm) liposomes resulted in extremely large (up to ~30 µm) liposomes in ALFQ, but AS01B liposomes remained small nano-vesicles. Here, we show that saturation of phospholipid chains is essential for production of large liposomes by QS21. 相似文献
3.
In budding yeast, the meiosis-specific protein kinase Ime2 is required for normal meiotic progression.Current evidence suggests that Ime2 is functionally related to Cdc28, the major cyclin-dependent kinase in yeastthat is essential for both cell cycle and meiosis. We have previously reported that a natural target of Ime2 activityis replication protein A (RPA), the cellular single-stranded DNA-binding protein that performs critical functionsduring DNA replication, repair, and recombination. Ime2-dependent RPA phosphorylation first occursearly in meiosis and targets the middle subunit of the RPA heterotrimeric complex (Rfa2). We now demonstratethat Rfa2 serine 27 (S27) is required for Ime2-dependent Rfa2 phosphorylation in vivo. S27 is also required forRfa2 phosphorylation in vitro catalyzed by immunoprecipitated Ime2. In addition, Ime2 mediates in vitro phosphorylationof a short peptide containing Rfa2 amino acids 23 through 29, thereby providing evidence that S27itself is the phosphoacceptor. Phosphorylation site mapping supports this conclusion, as mass spectrometryanalysis has revealed that at least three residues within Rfa2 amino acids 2 through 35 become phosphorylatedspecifically during meiosis. Although S27 is embedded in a motif that is recognized by several protein kinases,this sequence is not a typical target of cyclin-dependent kinases. Therefore, the mechanism underlying Ime2substrate recognition could differ from that of Cdc28. 相似文献
4.
菌体的分泌蛋白质在宿主和菌体的相互作用之间起着重要的作用. 本研究采用双向凝胶电泳的方法建立了长双歧杆菌XY01分泌蛋白质图谱,通过MALDI-TOF/TOF质 谱鉴定和数据库搜索,对鉴定到的分泌蛋白进行了分析. 共检测到21个蛋白质点, 成功鉴定18个蛋白质点,分别代表14个不同的蛋白质,等电点分布在4.5~7.0之间 ,分子质量分布在20 ~65 kD之间;通过COGs分类和功能分析,信号肽和细胞定位及KEGG代谢通路分析. 结果表明,这些蛋白质对菌体细胞壁/膜的形成、生物信号传导和物质代谢等起着重要作用. 研究结果为长双歧杆菌蛋白质组学和基因组学的研究提供了参考. 相似文献
5.
为研究在饲料中添加凝结芽胞杆菌BC01对白羽肉鸡生产性能、免疫器官指数及肠道大肠埃希菌的影响,将240只白羽肉鸡随机分为4组,试验组1、试验组2、试验组3在饲料中添加凝结芽胞杆菌BC01的终浓度分别为1×10~8、2×10~8、3×10~8 cfu/kg,对照组日粮中不添加任何微生态制剂,饲养6周。结果显示,日粮中添加凝结芽胞杆菌BC01可显著提高肉鸡平均日增重(P0.05),显著降低料重比(P0.05),降低死淘率(P0.05),胸腺指数、法氏囊指数均显著高于对照组(P0.05),降低肠道中的大肠埃希菌数量。结果表明,日粮中添加凝结芽胞杆菌BC01,对肉鸡的生长性能有一定的促进作用,提高饲料转化率,提升肉鸡的免疫力,提高肉鸡饲养的经济价值。凝结芽胞杆菌BC01的最佳添加浓度为每公斤饲料添加2×10~8 cfu/kg。 相似文献
6.
Silambarasan Tamil Selvan Balasubramani Govindasamy Sanjivkumar Muthusamy 《International journal of phytoremediation》2019,21(13):1305-1322
AbstractThis study deals with the open pond (OP) pilot scale treatment of cassava effluent and enhancement of Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) enzyme through CO2 utilization by the microalga, Acutodesmus obliquus RDS01. The cassava effluent treatment (ET) revealed maximum reduction of ammonia (96.8%), calcium (94.6%), chloride (95.2%), chlorine (98.5%), inorganic phosphate (94.6%), magnesium (96.8%), nitrate (96.89%), organic carbon (95.9%), organic phosphorus (96.3%), potassium (97.9%), sodium (97.1%), and sulfate (95.4%) on 15th day using A. obliquus. The microalga produced highest RuBisCO enzyme activity (90%), CO2 utilization efficiency (95%), biomass (8.9 gL?1), lipid (176.65?mg mL?1), carbohydrate (96.78?mg mL?1), biodiesel (4.1?mL g?1), and bioethanol (3.7?mL g?1) during OP treatment. The isolated RuBisCO gene (rbcL) was used to construct the protein model by homology modeling. The microalgal-lipid content was analyzed through thin layer chromatography, the biodiesel produced was analyzed using Fourier-transform infrared spectroscopy and gas chromatography mass spectrometry (GCMS). The bioethanol production was confirmed by high performance liquid chromatography and GCMS analyses. A. obliquus produced of 98.75% biodiesel and 96.83% bioethanol in the OP pilot scale treatment A. obliquus. Overall, the microalga A. obliquus could act as an effective CO2 capturing and bioremediation agent in the cassava ET, and also for the biodiesel and bioethanol can be produced. 相似文献
7.
8.
Ci Su-Qin Chen Shan Dong-Bo Liu Hong-Mei Xia 《World journal of microbiology & biotechnology》2006,22(7):729-735
Summary
Penicillium sp. DS9713a-01 was obtained by ultraviolet (u.v.) light mutagenesis from the Penicillium sp. DS9713a which can degrade poly (3-hydroxybutyrate) (PHB). The enzymatic activity of DS9713a-01 was 97% higher than that
of the wild-type strain. The DS9713a-01 mutant could completely degrade PHB films in 5 days; however, the wild-type strain
achieved only 61% at the same time. The extracellular PHB depolymerase was purified from the culture medium containing PHB
as the sole carbon source by filtration, ammonium sulfate precipitation and chromatography on Sepharose CL-6B. The molecular
weight of the PHB depolymerase was about 15.1kDa determined by SDS-polyacrylamide gel electrophoresis. The optimum activity
of the PHB depolymerase was observed at pH 8.6 and 50 °C. The enzyme was stable at temperatures below 37 °C and in the pH
range from 8.0 to 9.2. The activity of PHB depolymerase could be activated or inhibited by some metal ions. The apparent K
m
value was 0.164 mg ml−1. Mass spectrometric analysis of the water-soluble products after enzymatic degradation revealed that the primary product
was the monomer, 3-hydroxybutyric acid. 相似文献
9.
FS2M01.pl,转换片段大小表为0/1矩阵的Perl脚本 总被引:1,自引:0,他引:1
用Perl语言编写了一个脚本———FS2M01.pl来实现片段大小表向0/1矩阵的转换,解决了由人工方法将遗传图谱的位点信息转换成0/1矩阵的问题。 相似文献
10.