Power production in MFCs inoculated with Shewanella oneidensis MR‐1 or mixed cultures

Power densities and oxidation–reduction potentials (ORPs) of MFCs containing a pure culture of Shewanella oneidensis MR‐1 were compared to mixed cultures (wastewater inoculum) in cube shaped, 1‐, 2‐, and 3‐bottle batch‐fed MFC reactor configurations. The reactor architecture influenced the relative power produced by the different inocula, with the mixed culture generating 68–480% more power than MR‐1 in each MFC configuration. The mixed culture produced the maximum power density of 858 ± 9 mW m^?2 in the cubic MFC, while MR‐1 produced 148 ± 20 mW m^?2. The higher power by the mixed culture was primarily a result of lower internal resistances than those produced by the pure culture. Power was a direct function of ohmic resistance for the mixed culture, but not for strain MR‐1. ORP of the anode compartment varied with reactor configuration and inoculum, and it was always negative during maximum power production but it did not vary in proportion to power output. The ORP varied primarily at the end of the cycle when substrate was depleted, with a change from a reductive environment during maximum power production (approximately ?175 mV for mixed and approximately ?210 mV for MR‐1 in cubic MFCs), to an oxidative environment at the end of the batch cycle (～250 mV for mixed and ～300 mV for MR‐1). Mixed cultures produced more power than MR‐1 MFCs even though their redox potential was less negative. These results demonstrate that differences between power densities produced by pure and mixed cultures depend on the MFC architecture. Biotechnol. Bioeng. 2010; 105: 489–498. © 2009 Wiley Periodicals, Inc.     

Opportunities for renewable bioenergy using microorganisms

Global warming can be slowed, and perhaps reversed, only when society replaces fossil fuels with renewable, carbon-neutral alternatives. The best option is bioenergy: the sun's energy is captured in biomass and converted to energy forms useful to modern society. To make a dent in global warming, bioenergy must be generated at a very high rate, since the world today uses approximately 10 TW of fossil-fuel energy. And, it must do so without inflicting serious damage on the environment or disrupting our food supply. While most bioenergy options fail on both counts, several microorganism-based options have the potential to produce large amounts of renewable energy without disruptions. In one approach, microbial communities convert the energy value of various biomass residuals to socially useful energy. Biomass residuals come from agricultural, animal, and a variety of industrial operations, as well as from human wastes. Microorganisms can convert almost all of the energy in these wastes to methane, hydrogen, and electricity. In a second approach, photosynthetic microorganisms convert sunlight into biodiesel. Certain algae (eukaryotes) or cyanobacteria (prokaryotes) have high lipid contents. Under proper conditions, these photosynthetic microorganisms can produce lipids for biodiesel with yields per unit area 100 times or more than possible with any plant system. In addition, the non-lipid biomass can be converted to methane, hydrogen, or electricity. Photosynthetic microorganisms do not require arable land, an advantage because our arable land must be used to produce food. Algae or cyanobacteria may be the best option to produce bioenergy at rates high enough to replace a substantial fraction of our society's use of fossil fuels.     

Photosynthetic microbial fuel cells with positive light response

The current study introduces an aerobic single‐chamber photosynthetic microbial fuel cell (PMFC). Evaluation of PMFC performance using naturally growing fresh‐water photosynthetic biofilm revealed a weak positive light response, that is, an increase in cell voltage upon illumination. When the PMFC anodes were coated with electrically conductive polymers, the rate of voltage increased and the amplitude of the light response improved significantly. The rapid immediate positive response to light was consistent with a mechanism postulating that the photosynthetic electron‐transfer chain is the source of the electrons harvested on the anode surface. This mechanism is fundamentally different from the one exploited in previously designed anaerobic microbial fuel cells (MFCs), sediment MFCs, or anaerobic PMFCs, where the electrons are derived from the respiratory electron‐transfer chain. The power densities produced in PMFCs were substantially lower than those that are currently reported for conventional MFC (0.95 mW/m² for polyaniline‐coated and 1.3 mW/m² for polypyrrole‐coated anodes). However, the PMFC did not depend on an organic substrate as an energy source and was powered only by light energy. Its operation was CO₂‐neutral and did not require buffers or exogenous electron transfer shuttles. Biotechnol. Bioeng. 2009; 104: 939–946. © 2009 Wiley Periodicals, Inc.     

Comparative bioelectrochemical analysis of Pseudomonas aeruginosa and Escherichia coli with anaerobic consortia as anodic biocatalyst for biofuel cell application

Aims: To study the bioelectrochemical behaviour of Pseudomonas aeruginosa (MTCC 17702) and Escherichia coli (MTCC 10436) and to assess their potential to act as anodic biocatalyst with the function of anaerobic consortia for microbial (bio) fuel cell (BFC) application. Methods and Results: Three BFCs (single chamber; open‐air cathode; noncatalysed electrodes) were operated simultaneously in acidophilic microenvironments. Pseudomonas aeruginosa (BFC_P) showed higher current density (264 mA m^?2) followed by mixed culture (BFC_M; 166 mA m^?2) and E. coli (BFC_E; 147 mA m^?2). However, total operating period and substrate degradation were relatively found to be effective with mixed culture (58%; 72 h) followed by BFC_P (39%; 60 h) and BFC_E (31%; 48 h). Higher electron discharge (ED) was observed with Ps. aeruginosa while mixed culture showed the involvement of redox mediators in the ED process. Conclusions: Mixed culture showed to sustain biopotential for longer periods along with a stable ED. The presence of redox signals and high substrate degradation was also evidencing its performance compared to the pure strains studied. This supports the practical utility of mixed culture over the pure cultures for real‐field BFC applications especially while operating with wastewater. Significance and Impact of the Study: This study revealed the efficiency and viability of mixed consortia in comparison with pure strains for microbial (bio) fuel cell applications.     

豚鼠离体腹腔神经节神经元自发f-EPSP和AP

应用细胞内生物电记录技术观察豚鼠腹腔神经节(CG)神经元自发快兴奋性突触后电位(f-EPSP)和动作电位(AP)的特征,分析其形成的可能机制。发现在豚鼠离体CG上存在自发f-EPSP和AP,发放频率不同。自发f-EPSP的幅度(5.67±2.66)mV(n=26),明显低于刺激内脏大神经诱发f-EPSP的幅度(13.26±6.74)mV(n=34,P<0.01),而自发的AP后超极化幅度(AHPA)(13.86±4.24)mV(n=30),明显高于刺激内脏大神经诱发的AHPA(8.99±2.79)mV(n=54,P<0.01)。六烃季铵或低Ca2 /高Mg2 Krebs液能完全阻断自发的AP,但自发的f-EPSP则不被完全阻断。结果提示豚鼠离体CG神经元有自发性电活动,这除与突触前膜ACh的随机释放有关以外,可能还有对Ca2 不敏感的其他递质介导。     

Environmental impacts of future bioenergy pathways: the case of electricity from wheat straw bales and pellets

This study presents the life cycle assessment of electricity generation from straw bales and pellets. Straw is the most abundant biomass residue in Europe and its use for energy purposes is promoted on the premise of high greenhouse gas savings. This assumption has delayed the study of sustainability of straw‐fired systems on a broader sense and the literature on the topic is almost absent. This study uses data from specific literature and emissions inventories to model a number of straw pathways. The plant modeled is a medium‐scale straw‐fired power plant of 50 MWth capacity. The results show that electricity from straw‐fired power plants can indeed realize high greenhouse gas savings compared both with existing coal plants and with the European electricity mix. The savings are in the range 70–94%. The influence of the geographical origin of straw is analyzed by using datasets for the cultivation of wheat in five different European countries. The highest emissions are recorded for the case of straw from Spain due to the small yields, whereas cultivation processes in United Kingdom and the Netherlands show high environmental impacts due to the high level of fertilization. Other environmental impacts are evaluated, such as acidification potential, eutrophication, particulate matter emissions, and photochemical ozone formation. The bioenergy system scores worse than the current European electricity mix for all the categories. However, it is important to notice that in Spain and United Kingdom the straw system shows lower impacts compared with the local average coal electricity. Finally, the study investigates the ‘break‐even’ distance at which the higher emissions from the pellets production are paid off by the saved emissions in their transport compared with the bales. The results show that no reasonable break‐even distance exists for road transport, whereas advantages for pellets are evident in any configuration for transoceanic transport.     

Molecular bioelectricity in developmental biology: new tools and recent discoveries: control of cell behavior and pattern formation by transmembrane potential gradients

Significant progress in the molecular investigation of endogenous bioelectric signals during pattern formation in growing tissues has been enabled by recently developed techniques. Ion flows and voltage gradients produced by ion channels and pumps are key regulators of cell proliferation, migration, and differentiation. Now, instructive roles for bioelectrical gradients in embryogenesis, regeneration, and neoplasm are being revealed through the use of fluorescent voltage reporters and functional experiments using well-characterized channel mutants. Transmembrane voltage gradients (V(mem) ) determine anatomical polarity and function as master regulators during appendage regeneration and embryonic left-right patterning. A state-of-the-art recent study reveals that they can also serve as prepatterns for gene expression domains during craniofacial patterning. Continued development of novel tools and better ways to think about physical controls of cell-cell interactions will lead to mastery of the morphogenetic information stored in physiological networks. This will enable fundamental advances in basic understanding of growth and form, as well as transformative biomedical applications in regenerative medicine.     

Demand for biomass to meet renewable energy targets in the United States: implications for land use

Renewable energy policies in the electricity and transportation sectors in the United States are expected to create demand for biomass and food crops (corn) that could divert land from food crop production. We develop a dynamic, open‐economy, price‐endogenous multi‐market model of the US agricultural, electricity and transportation sectors to endogenously determine the quantity and mix of bioenergy likely to be required to meet the state Renewable Portfolio Standards (RPSs) and the federal Renewable Fuel Standard (RFS) if implemented independently or jointly (RFS & RPS) over the 2007–2030 period and their implications for the extent and spatial pattern of diversion of land from other uses for biomass feedstock production. We find that the demand for biomass ranges from 100 million metric tons (MMT) under the RPS alone to 310 MMT under the RFS & RPS; 70% of the biomass in the latter case can be met by crop and forest residues, while the rest can be met by devoting 3% of cropland to energy crop production with 80% of this being marginal land. Our findings show significant potential to meet current renewable energy goals by expanding high‐yielding energy crop production on marginal land and using residues without conflicting with food crop production.     

Agronomic and environmental implications of sugarcane straw removal: a major review

Large‐scale bioenergy demand has triggered new approaches to straw management in Brazilian sugarcane fields. With the progressive shift from a burned to a nonburned harvest system, most of the straw presently retained on the soil surface has become economically viable feedstock for bioenergy production. The trade‐offs between the need to preserve soil quality and produce more bioenergy have been the subject of intense discussion. This study presents a synthesis of available information on the magnitude of the main impacts of straw removal from sugarcane fields for bioenergy production and therefore represents an easily available resource to guide management decisions on the recommended amount of straw to be maintained on the field to take advantage of the agronomic, environmental, and industrial benefits. Crop residues remaining on sugarcane fields provide numerous ecosystem services including nutrient recycling, soil biodiversity, water storage, carbon accumulation, control of soil erosion, and weed infestation. Furthermore, several studies reported higher sugarcane production under straw retention on the field, while few suggest that straw may jeopardize biomass production in cold regions and under some specific soil conditions. Pest control is among the parameters favored by straw removal, while N₂O emissions are increased only if straw is associated with the application of N fertilizer and vinasse. An appropriate recommendation, which is clearly site specific, should be based on a minimum mass of straw on the field to provide those benefits. Overall, this review indicates that most of the agronomic and environmental benefits are achieved when at least 7 Mg ha^?1 of dry straw is maintained on the soil surface. However, modeling efforts are of paramount importance to assess the magnitude and rates of straw removal considering the several indicators involved in this complex equation, so that an accurate straw recovery rate could be provided to producers and industry toward greater sustainability.